Skip to main content

Cytokine profiles in seronegative volunteers immunized with a recombinant canarypox and gp120 prime-boost HIV-1 vaccine. NIAID AIDS Vaccine Evaluation Group.

Publication ,  Other
Sabbaj, S; Mulligan, MJ; Hsieh, RH; Belshe, RB; McGhee, JR
Published in: AIDS
July 7, 2000

OBJECTIVES: To study memory T cell proliferative responses and cytokine profiles induced in HIV-1 seronegative volunteers immunized with a live recombinant canarypox vector expressing HIV-1 antigens (ALVAC-HIV) and boosted with a recombinant gp120 subunit vaccine. DESIGN: HIV-specific T cell proliferative responses and cytokines were measured 2 weeks after vaccination. Cytokines secreted by T helper 1 cells (Th1) [interleukin (IL)-2 and interferon-gamma (IFN-gamma)] and T helper 2 (Th2) cells (IL-4, IL-5, IL-6, and IL-10) were assessed both at the mRNA and the protein level. METHODS: Peripheral blood mononuclear cells (PBMC) were stimulated in vitro with HIV antigens. Subsequently, T cell proliferation was measured in a standard lymphoproliferation assay; secreted cytokines were measured using an enzyme-linked immunosorbent assay and upregulation of cytokine mRNA was measured using reverse transcriptase polymerase chain reaction. RESULTS: All individuals who had received ALVAC-HIV followed by the protein vaccine exhibited HIV-1-specific T cell proliferative responses. Moreover, the PBMC of all prime-boost vaccinated individuals produced detectable IFN-gamma and IL-10 in response to stimulation with HIV-1 envelope glycoprotein antigens; 83% also had detectable levels of IL-2 and IL-6, 71% had detectable levels of IL-4, and 86% had detectable levels of IL-5. CONCLUSIONS: These data indicate that this vaccination regimen was inducing both Th1- and Th2-type responses to HIV-1 envelope antigens. This prime-boost vaccination approach elicited T cell help for the generation of cytotoxic T lymphocyte responses as well as help for antibody production and so promises to generate a broad HIV-1-specific immune response.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

AIDS

DOI

ISSN

0269-9370

Publication Date

July 7, 2000

Volume

14

Issue

10

Start / End Page

1365 / 1374

Location

England

Related Subject Headings

  • Virology
  • Vaccines, Synthetic
  • T-Lymphocytes, Cytotoxic
  • T-Lymphocytes
  • RNA, Messenger
  • Neutralization Tests
  • Middle Aged
  • Lymphocyte Activation
  • In Vitro Techniques
  • Immunologic Memory
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Sabbaj, S., Mulligan, M. J., Hsieh, R. H., Belshe, R. B., & McGhee, J. R. (2000). Cytokine profiles in seronegative volunteers immunized with a recombinant canarypox and gp120 prime-boost HIV-1 vaccine. NIAID AIDS Vaccine Evaluation Group. AIDS. England. https://doi.org/10.1097/00002030-200007070-00009
Sabbaj, S., M. J. Mulligan, R. H. Hsieh, R. B. Belshe, and J. R. McGhee. “Cytokine profiles in seronegative volunteers immunized with a recombinant canarypox and gp120 prime-boost HIV-1 vaccine. NIAID AIDS Vaccine Evaluation Group.AIDS, July 7, 2000. https://doi.org/10.1097/00002030-200007070-00009.
Sabbaj, S., et al. “Cytokine profiles in seronegative volunteers immunized with a recombinant canarypox and gp120 prime-boost HIV-1 vaccine. NIAID AIDS Vaccine Evaluation Group.AIDS, vol. 14, no. 10, 7 July 2000, pp. 1365–74. Pubmed, doi:10.1097/00002030-200007070-00009.

Published In

AIDS

DOI

ISSN

0269-9370

Publication Date

July 7, 2000

Volume

14

Issue

10

Start / End Page

1365 / 1374

Location

England

Related Subject Headings

  • Virology
  • Vaccines, Synthetic
  • T-Lymphocytes, Cytotoxic
  • T-Lymphocytes
  • RNA, Messenger
  • Neutralization Tests
  • Middle Aged
  • Lymphocyte Activation
  • In Vitro Techniques
  • Immunologic Memory