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Variation in chromosome copy number influences the virulence of Cryptococcus neoformans and occurs in isolates from AIDS patients.

Publication ,  Journal Article
Hu, G; Wang, J; Choi, J; Jung, WH; Liu, I; Litvintseva, AP; Bicanic, T; Aurora, R; Mitchell, TG; Perfect, JR; Kronstad, JW
Published in: BMC Genomics
October 27, 2011

BACKGROUND: The adaptation of pathogenic fungi to the host environment via large-scale genomic changes is a poorly characterized phenomenon. Cryptococcus neoformans is the leading cause of fungal meningoencephalitis in HIV/AIDS patients, and we recently discovered clinical strains of the fungus that are disomic for chromosome 13. Here, we examined the genome plasticity and phenotypes of monosomic and disomic strains, and compared their virulence in a mouse model of cryptococcosis RESULTS: In an initial set of strains, melanin production was correlated with monosomy at chromosome 13, and disomic variants were less melanized and attenuated for virulence in mice. After growth in culture or passage through mice, subsequent strains were identified that varied in melanin formation and exhibited copy number changes for other chromosomes. The correlation between melanin and disomy at chromosome 13 was observed for some but not all strains. A survey of environmental and clinical isolates maintained in culture revealed few occurrences of disomic chromosomes. However, an examination of isolates that were freshly collected from the cerebrospinal fluid of AIDS patients and minimally cultured provided evidence for infections with multiple strains and copy number variation. CONCLUSIONS: Overall, these results suggest that the genome of C. neoformans exhibits a greater degree of plasticity than previously appreciated. Furthermore, the expression of an essential virulence factor and the severity of disease are associated with genome variation. The occurrence of chromosomal variation in isolates from AIDS patients, combined with the observed influence of disomy on virulence, indicates that genome plasticity may have clinical relevance.

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Published In

BMC Genomics

DOI

EISSN

1471-2164

Publication Date

October 27, 2011

Volume

12

Start / End Page

526

Location

England

Related Subject Headings

  • Virulence Factors
  • Virulence
  • Mice, Inbred BALB C
  • Mice
  • Melanins
  • Humans
  • Female
  • DNA Copy Number Variations
  • Cryptococcus neoformans
  • Cryptococcosis
 

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Hu, G., Wang, J., Choi, J., Jung, W. H., Liu, I., Litvintseva, A. P., … Kronstad, J. W. (2011). Variation in chromosome copy number influences the virulence of Cryptococcus neoformans and occurs in isolates from AIDS patients. BMC Genomics, 12, 526. https://doi.org/10.1186/1471-2164-12-526
Hu, Guanggan, Joyce Wang, Jaehyuk Choi, Won Hee Jung, Iris Liu, Anastasia P. Litvintseva, Tihana Bicanic, et al. “Variation in chromosome copy number influences the virulence of Cryptococcus neoformans and occurs in isolates from AIDS patients.BMC Genomics 12 (October 27, 2011): 526. https://doi.org/10.1186/1471-2164-12-526.
Hu G, Wang J, Choi J, Jung WH, Liu I, Litvintseva AP, et al. Variation in chromosome copy number influences the virulence of Cryptococcus neoformans and occurs in isolates from AIDS patients. BMC Genomics. 2011 Oct 27;12:526.
Hu, Guanggan, et al. “Variation in chromosome copy number influences the virulence of Cryptococcus neoformans and occurs in isolates from AIDS patients.BMC Genomics, vol. 12, Oct. 2011, p. 526. Pubmed, doi:10.1186/1471-2164-12-526.
Hu G, Wang J, Choi J, Jung WH, Liu I, Litvintseva AP, Bicanic T, Aurora R, Mitchell TG, Perfect JR, Kronstad JW. Variation in chromosome copy number influences the virulence of Cryptococcus neoformans and occurs in isolates from AIDS patients. BMC Genomics. 2011 Oct 27;12:526.
Journal cover image

Published In

BMC Genomics

DOI

EISSN

1471-2164

Publication Date

October 27, 2011

Volume

12

Start / End Page

526

Location

England

Related Subject Headings

  • Virulence Factors
  • Virulence
  • Mice, Inbred BALB C
  • Mice
  • Melanins
  • Humans
  • Female
  • DNA Copy Number Variations
  • Cryptococcus neoformans
  • Cryptococcosis