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Characterization of lens fiber cell triton insoluble fraction reveals ERM (ezrin, radixin, moesin) proteins as major cytoskeletal-associated proteins.

Publication ,  Journal Article
Rao, PV; Ho, T; Skiba, NP; Maddala, R
Published in: Biochem Biophys Res Commun
April 11, 2008

To understand lens fiber cell elongation- and differentiation-associated cytoskeletal remodeling, here we identified and characterized the major protein components of lens fiber cell Triton X-100 insoluble fraction by mass spectrometry and immunoblot analysis. This analysis identified spectrin, filensin, vimentin, tubulin, phakinin, and beta-actin as major cytoskeletal proteins in the lens fibers. Importantly, ezrin, radixin, and moesin (ERM), heat-shock cognate protein 70, and beta/gamma-crystallins were identified as major cytoskeletal-associated proteins. ERM proteins were confirmed to exist as active phosphorylated forms that exhibited intense distribution in the organelle free-zone fibers. Furthermore, ERM protein phosphorylation was found to be dramatically reduced in Rho GTPase-targeted transgenic mouse lenses. These data identify the ERM proteins, which cross-link the plasma membrane and actin, as major and stable cytoskeletal-associated proteins in lens fibers, and indicate a potential role(s) for the ERMs in fiber cell actin cytoskeletal and membrane organization.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

EISSN

1090-2104

Publication Date

April 11, 2008

Volume

368

Issue

3

Start / End Page

508 / 514

Location

United States

Related Subject Headings

  • Solubility
  • Octoxynol
  • Microfilament Proteins
  • Mice
  • Membrane Proteins
  • Lens, Crystalline
  • Cytoskeleton
  • Cytoskeletal Proteins
  • Cells, Cultured
  • Cell-Free System
 

Citation

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MLA
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Rao, P. V., Ho, T., Skiba, N. P., & Maddala, R. (2008). Characterization of lens fiber cell triton insoluble fraction reveals ERM (ezrin, radixin, moesin) proteins as major cytoskeletal-associated proteins. Biochem Biophys Res Commun, 368(3), 508–514. https://doi.org/10.1016/j.bbrc.2008.01.128
Rao, P Vasantha, Tammy Ho, Nikolai P. Skiba, and Rupalatha Maddala. “Characterization of lens fiber cell triton insoluble fraction reveals ERM (ezrin, radixin, moesin) proteins as major cytoskeletal-associated proteins.Biochem Biophys Res Commun 368, no. 3 (April 11, 2008): 508–14. https://doi.org/10.1016/j.bbrc.2008.01.128.
Rao, P. Vasantha, et al. “Characterization of lens fiber cell triton insoluble fraction reveals ERM (ezrin, radixin, moesin) proteins as major cytoskeletal-associated proteins.Biochem Biophys Res Commun, vol. 368, no. 3, Apr. 2008, pp. 508–14. Pubmed, doi:10.1016/j.bbrc.2008.01.128.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

EISSN

1090-2104

Publication Date

April 11, 2008

Volume

368

Issue

3

Start / End Page

508 / 514

Location

United States

Related Subject Headings

  • Solubility
  • Octoxynol
  • Microfilament Proteins
  • Mice
  • Membrane Proteins
  • Lens, Crystalline
  • Cytoskeleton
  • Cytoskeletal Proteins
  • Cells, Cultured
  • Cell-Free System