Bacterial flavohemoglobin: a molecular tool to probe mammalian nitric oxide biology.
A wide range of mammalian signaling and stress pathways are mediated by nitric oxide (NO), which is synthesized in vivo by the nitric oxide synthase (NOS) family of enzymes. Experimental manipulations of NO are frequently achieved by either inhibition or activation of endogenous NOS or via providing exogenous NO sources. On the contrary, many microbes consume NO via flavohemoglobin (FlavoHb), a highly efficient NO-dioxygenase that protects from nitrosative stress. Here we report a novel resource for studying NO in mammalian cells by heterologously expressing Escherichia coli FlavoHb within a lentiviral delivery system. This technique boosts endogenous cellular consumption of NO, thus providing a simple and efficacious approach to studying mammalian NO biology that can be employed as both a primary experimental and confirmatory tool.
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- Transfection
- Signal Transduction
- Oxidation-Reduction
- Nitric Oxide Synthase Type II
- Nitric Oxide
- NADH, NADPH Oxidoreductases
- Lentivirus
- Humans
- Hemeproteins
- HEK293 Cells
Citation
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Transfection
- Signal Transduction
- Oxidation-Reduction
- Nitric Oxide Synthase Type II
- Nitric Oxide
- NADH, NADPH Oxidoreductases
- Lentivirus
- Humans
- Hemeproteins
- HEK293 Cells