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The chorion layer of fetal membranes is prematurely destroyed in women with preterm premature rupture of the membranes.

Publication ,  Journal Article
Canzoneri, BJ; Feng, L; Grotegut, CA; Bentley, RC; Heine, RP; Murtha, AP
Published in: Reprod Sci
October 2013

Preterm premature rupture of the membranes (PPROM) is an important etiology of preterm birth and source of significant neonatal morbidity. We propose that PPROM occurs in the setting of long-standing altered tissue remodeling, which creates a vulnerable environment for the fetal membranes and pregnancy. We tested the hypothesis that PPROM is the result of tissue remodeling in the fetal membranes, specifically the chorion, and this weakening of the chorion compromises the protection provided to the amnion. The purpose of this study was to quantify thickness and apoptosis in the choriodecidua of fetal membranes in patients with PPROM, preterm labor (PTL), preterm no labor (PTNL), and women with term labor (TERM). We conducted a retrospective evaluation of fetal membrane samples from 86 placentas. Immunohistochemistry was performed using a cytokeratin antibody, and mean chorion cellular thickness was compared between each clinical group. To evaluate chorion apoptosis, fetal membranes from patients with PPROM, PTL, and TERM were stained with the M30 antibody, and the degree of cellular apoptosis was determined. Statistical analysis was performed using analysis of variance with corrections for multiple comparisons. The chorion cellular layer was thinner in patients with PPROM compared to patients with PTNL and TERM (62, 140, and 169 µm, respectively, P < .0001), though not significantly different from PTL (95 µm, P > .05). The percentage of apoptotic cells within the chorion among the patients with PPROM was greater compared to PTL and TERM (24.2%, 13.1%, and 8.4%, respectively, P < .001). The chorion cellular layer is thinner and demonstrates increased apoptosis in PPROM compared to patients with PTL, PTNL, and TERM, suggesting differential remodeling between clinical phenotypes.

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Published In

Reprod Sci

DOI

EISSN

1933-7205

Publication Date

October 2013

Volume

20

Issue

10

Start / End Page

1246 / 1254

Location

United States

Related Subject Headings

  • Young Adult
  • Retrospective Studies
  • Pregnancy
  • Obstetrics & Reproductive Medicine
  • Humans
  • Fetal Membranes, Premature Rupture
  • Female
  • Extraembryonic Membranes
  • Cohort Studies
  • Chorion
 

Citation

APA
Chicago
ICMJE
MLA
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Canzoneri, B. J., Feng, L., Grotegut, C. A., Bentley, R. C., Heine, R. P., & Murtha, A. P. (2013). The chorion layer of fetal membranes is prematurely destroyed in women with preterm premature rupture of the membranes. Reprod Sci, 20(10), 1246–1254. https://doi.org/10.1177/1933719113483009
Canzoneri, Bernard J., Liping Feng, Chad A. Grotegut, Rex C. Bentley, R Phillips Heine, and Amy P. Murtha. “The chorion layer of fetal membranes is prematurely destroyed in women with preterm premature rupture of the membranes.Reprod Sci 20, no. 10 (October 2013): 1246–54. https://doi.org/10.1177/1933719113483009.
Canzoneri BJ, Feng L, Grotegut CA, Bentley RC, Heine RP, Murtha AP. The chorion layer of fetal membranes is prematurely destroyed in women with preterm premature rupture of the membranes. Reprod Sci. 2013 Oct;20(10):1246–54.
Canzoneri, Bernard J., et al. “The chorion layer of fetal membranes is prematurely destroyed in women with preterm premature rupture of the membranes.Reprod Sci, vol. 20, no. 10, Oct. 2013, pp. 1246–54. Pubmed, doi:10.1177/1933719113483009.
Canzoneri BJ, Feng L, Grotegut CA, Bentley RC, Heine RP, Murtha AP. The chorion layer of fetal membranes is prematurely destroyed in women with preterm premature rupture of the membranes. Reprod Sci. 2013 Oct;20(10):1246–1254.
Journal cover image

Published In

Reprod Sci

DOI

EISSN

1933-7205

Publication Date

October 2013

Volume

20

Issue

10

Start / End Page

1246 / 1254

Location

United States

Related Subject Headings

  • Young Adult
  • Retrospective Studies
  • Pregnancy
  • Obstetrics & Reproductive Medicine
  • Humans
  • Fetal Membranes, Premature Rupture
  • Female
  • Extraembryonic Membranes
  • Cohort Studies
  • Chorion