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Three-in-one chromatography-free purification, tag removal, and site-specific modification of recombinant fusion proteins using sortase A and elastin-like polypeptides.

Publication ,  Journal Article
Bellucci, JJ; Amiram, M; Bhattacharyya, J; McCafferty, D; Chilkoti, A
Published in: Angewandte Chemie (International ed. in English)
March 2013

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Published In

Angewandte Chemie (International ed. in English)

DOI

EISSN

1521-3773

ISSN

1433-7851

Publication Date

March 2013

Volume

52

Issue

13

Start / End Page

3703 / 3708

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • TNF-Related Apoptosis-Inducing Ligand
  • Staphylococcus aureus
  • Recombinant Fusion Proteins
  • Organic Chemistry
  • Mutagenesis, Site-Directed
  • Mice
  • Humans
  • Escherichia coli
  • Elastin
 

Citation

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Bellucci, J. J., Amiram, M., Bhattacharyya, J., McCafferty, D., & Chilkoti, A. (2013). Three-in-one chromatography-free purification, tag removal, and site-specific modification of recombinant fusion proteins using sortase A and elastin-like polypeptides. Angewandte Chemie (International Ed. in English), 52(13), 3703–3708. https://doi.org/10.1002/anie.201208292
Bellucci, Joseph J., Miriam Amiram, Jayanta Bhattacharyya, Dewey McCafferty, and Ashutosh Chilkoti. “Three-in-one chromatography-free purification, tag removal, and site-specific modification of recombinant fusion proteins using sortase A and elastin-like polypeptides.Angewandte Chemie (International Ed. in English) 52, no. 13 (March 2013): 3703–8. https://doi.org/10.1002/anie.201208292.
Bellucci JJ, Amiram M, Bhattacharyya J, McCafferty D, Chilkoti A. Three-in-one chromatography-free purification, tag removal, and site-specific modification of recombinant fusion proteins using sortase A and elastin-like polypeptides. Angewandte Chemie (International ed in English). 2013 Mar;52(13):3703–8.
Bellucci, Joseph J., et al. “Three-in-one chromatography-free purification, tag removal, and site-specific modification of recombinant fusion proteins using sortase A and elastin-like polypeptides.Angewandte Chemie (International Ed. in English), vol. 52, no. 13, Mar. 2013, pp. 3703–08. Epmc, doi:10.1002/anie.201208292.
Bellucci JJ, Amiram M, Bhattacharyya J, McCafferty D, Chilkoti A. Three-in-one chromatography-free purification, tag removal, and site-specific modification of recombinant fusion proteins using sortase A and elastin-like polypeptides. Angewandte Chemie (International ed in English). 2013 Mar;52(13):3703–3708.
Journal cover image

Published In

Angewandte Chemie (International ed. in English)

DOI

EISSN

1521-3773

ISSN

1433-7851

Publication Date

March 2013

Volume

52

Issue

13

Start / End Page

3703 / 3708

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • TNF-Related Apoptosis-Inducing Ligand
  • Staphylococcus aureus
  • Recombinant Fusion Proteins
  • Organic Chemistry
  • Mutagenesis, Site-Directed
  • Mice
  • Humans
  • Escherichia coli
  • Elastin