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Assessing the radiation response of lung cancer with different gene mutations using genetically engineered mice.

Publication ,  Journal Article
Perez, BA; Ghafoori, AP; Lee, C-L; Johnston, SM; Li, Y; Moroshek, JG; Ma, Y; Mukherjee, S; Kim, Y; Badea, CT; Kirsch, DG
Published in: Front Oncol
2013

PURPOSE: Non-small cell lung cancers (NSCLC) are a heterogeneous group of carcinomas harboring a variety of different gene mutations. We have utilized two distinct genetically engineered mouse models of human NSCLC (adenocarcinoma) to investigate how genetic factors within tumor parenchymal cells influence the in vivo tumor growth delay after one or two fractions of radiation therapy (RT). MATERIALS AND METHODS: Primary lung adenocarcinomas were generated in vivo in mice by intranasal delivery of an adenovirus expressing Cre-recombinase. Lung cancers expressed oncogenic Kras(G12D) and were also deficient in one of two tumor suppressor genes: p53 or Ink4a/ARF. Mice received no radiation treatment or whole lung irradiation in a single fraction (11.6 Gy) or in two 7.3 Gy fractions (14.6 Gy total) separated by 24 h. In each case, the biologically effective dose (BED) equaled 25 Gy10. Response to RT was assessed by micro-CT 2 weeks after treatment. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and immunohistochemical staining were performed to assess the integrity of the p53 pathway, the G1 cell-cycle checkpoint, and apoptosis. RESULTS: Tumor growth rates prior to RT were similar for the two genetic variants of lung adenocarcinoma. Lung cancers with wild-type (WT) p53 (LSL-Kras; Ink4a/ARF(FL/FL) mice) responded better to two daily fractions of 7.3 Gy compared to a single fraction of 11.6 Gy (P = 0.002). There was no statistically significant difference in the response of lung cancers deficient in p53 (LSL-Kras; p53(FL/FL) mice) to a single fraction (11.6 Gy) compared to 7.3 Gy × 2 (P = 0.23). Expression of the p53 target genes p21 and PUMA were higher and bromodeoxyuridine uptake was lower after RT in tumors with WT p53. CONCLUSION: Using an in vivo model of malignant lung cancer in mice, we demonstrate that the response of primary lung cancers to one or two fractions of RT can be influenced by specific gene mutations.

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Published In

Front Oncol

DOI

ISSN

2234-943X

Publication Date

2013

Volume

3

Start / End Page

72

Location

Switzerland

Related Subject Headings

  • 3211 Oncology and carcinogenesis
  • 3202 Clinical sciences
  • 1112 Oncology and Carcinogenesis
 

Citation

APA
Chicago
ICMJE
MLA
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Perez, B. A., Ghafoori, A. P., Lee, C.-L., Johnston, S. M., Li, Y., Moroshek, J. G., … Kirsch, D. G. (2013). Assessing the radiation response of lung cancer with different gene mutations using genetically engineered mice. Front Oncol, 3, 72. https://doi.org/10.3389/fonc.2013.00072
Perez, Bradford A., A Paiman Ghafoori, Chang-Lung Lee, Samuel M. Johnston, Yifan Li, Jacob G. Moroshek, Yan Ma, et al. “Assessing the radiation response of lung cancer with different gene mutations using genetically engineered mice.Front Oncol 3 (2013): 72. https://doi.org/10.3389/fonc.2013.00072.
Perez BA, Ghafoori AP, Lee C-L, Johnston SM, Li Y, Moroshek JG, et al. Assessing the radiation response of lung cancer with different gene mutations using genetically engineered mice. Front Oncol. 2013;3:72.
Perez, Bradford A., et al. “Assessing the radiation response of lung cancer with different gene mutations using genetically engineered mice.Front Oncol, vol. 3, 2013, p. 72. Pubmed, doi:10.3389/fonc.2013.00072.
Perez BA, Ghafoori AP, Lee C-L, Johnston SM, Li Y, Moroshek JG, Ma Y, Mukherjee S, Kim Y, Badea CT, Kirsch DG. Assessing the radiation response of lung cancer with different gene mutations using genetically engineered mice. Front Oncol. 2013;3:72.

Published In

Front Oncol

DOI

ISSN

2234-943X

Publication Date

2013

Volume

3

Start / End Page

72

Location

Switzerland

Related Subject Headings

  • 3211 Oncology and carcinogenesis
  • 3202 Clinical sciences
  • 1112 Oncology and Carcinogenesis