Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

Sortase-catalyzed initiator attachment enables high yield growth of a stealth polymer from the C terminus of a protein.

Publication ,  Journal Article
Qi, Y; Amiram, M; Gao, W; McCafferty, DG; Chilkoti, A
Published in: Macromolecular rapid communications
August 2013

Conventional methods for synthesizing protein/peptide-polymer conjugates, as a means to improve the pharmacological properties of therapeutic biomolecules, typically have drawbacks including low yield, non-trivial separation of conjugates from reactants, and lack of site- specificity, which results in heterogeneous products with significantly compromised bioactivity. To address these limitations, the use of sortase A from Staphylococcus aureus is demonstrated to site-specifically attach an initiator solely at the C-terminus of green fluorescent protein (GFP), followed by in situ growth of a stealth polymer, poly(oligo(ethylene glycol) methyl ether methacrylate) by atom transfer radical polymerization (ATRP). Sortase-catalyzed initiator attachment proceeds with high specificity and near-complete (≈95%) product conversion. Subsequent in situ ATRP in aqueous buffer produces 1:1 stoichiometric conjugates with >90% yield, low dispersity, and no denaturation of the protein. This approach introduces a simple and useful method for high yield synthesis of protein/peptide-polymer conjugates.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Macromolecular rapid communications

DOI

EISSN

1521-3927

ISSN

1022-1336

Publication Date

August 2013

Volume

34

Issue

15

Start / End Page

1256 / 1260

Related Subject Headings

  • Staphylococcus aureus
  • Polymers
  • Polyethylene Glycols
  • Green Fluorescent Proteins
  • Cysteine Endopeptidases
  • Bacterial Proteins
  • Aminoacyltransferases
  • 40 Engineering
  • 34 Chemical sciences
  • 09 Engineering
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Qi, Y., Amiram, M., Gao, W., McCafferty, D. G., & Chilkoti, A. (2013). Sortase-catalyzed initiator attachment enables high yield growth of a stealth polymer from the C terminus of a protein. Macromolecular Rapid Communications, 34(15), 1256–1260. https://doi.org/10.1002/marc.201300460
Qi, Yizhi, Miriam Amiram, Weiping Gao, Dewey G. McCafferty, and Ashutosh Chilkoti. “Sortase-catalyzed initiator attachment enables high yield growth of a stealth polymer from the C terminus of a protein.Macromolecular Rapid Communications 34, no. 15 (August 2013): 1256–60. https://doi.org/10.1002/marc.201300460.
Qi Y, Amiram M, Gao W, McCafferty DG, Chilkoti A. Sortase-catalyzed initiator attachment enables high yield growth of a stealth polymer from the C terminus of a protein. Macromolecular rapid communications. 2013 Aug;34(15):1256–60.
Qi, Yizhi, et al. “Sortase-catalyzed initiator attachment enables high yield growth of a stealth polymer from the C terminus of a protein.Macromolecular Rapid Communications, vol. 34, no. 15, Aug. 2013, pp. 1256–60. Epmc, doi:10.1002/marc.201300460.
Qi Y, Amiram M, Gao W, McCafferty DG, Chilkoti A. Sortase-catalyzed initiator attachment enables high yield growth of a stealth polymer from the C terminus of a protein. Macromolecular rapid communications. 2013 Aug;34(15):1256–1260.
Journal cover image

Published In

Macromolecular rapid communications

DOI

EISSN

1521-3927

ISSN

1022-1336

Publication Date

August 2013

Volume

34

Issue

15

Start / End Page

1256 / 1260

Related Subject Headings

  • Staphylococcus aureus
  • Polymers
  • Polyethylene Glycols
  • Green Fluorescent Proteins
  • Cysteine Endopeptidases
  • Bacterial Proteins
  • Aminoacyltransferases
  • 40 Engineering
  • 34 Chemical sciences
  • 09 Engineering