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Optical clearing and multiphoton imaging of paraffin-embedded specimens

Publication ,  Conference
Wilson, JW; Degan, S; Fischer, MC; Warren, WS
Published in: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
June 12, 2013

New labeling, imaging, or analysis tools could provide new retrospective insights when applied to archived, paraffinembedded samples. Deep-tissue multiphoton microscopy of paraffin-embedded specimens is achieved using optical clearing with mineral oil. We tested a variety of murine tissue specimens including skin, lung, spleen, kidney, and heart, acquiring multiphoton autofluorescence and second-harmonic generation, and pump-probe images This technique introduces the capability for non-destructive 3-dimensional microscopic imaging of existing archived pathology specimens, enabling retrospective studies. © 2013 SPIE.

Duke Scholars

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

Publication Date

June 12, 2013

Volume

8588
 

Citation

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Wilson, J. W., Degan, S., Fischer, M. C., & Warren, W. S. (2013). Optical clearing and multiphoton imaging of paraffin-embedded specimens. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8588). https://doi.org/10.1117/12.2003155
Wilson, J. W., S. Degan, M. C. Fischer, and W. S. Warren. “Optical clearing and multiphoton imaging of paraffin-embedded specimens.” In Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 8588, 2013. https://doi.org/10.1117/12.2003155.
Wilson JW, Degan S, Fischer MC, Warren WS. Optical clearing and multiphoton imaging of paraffin-embedded specimens. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2013.
Wilson, J. W., et al. “Optical clearing and multiphoton imaging of paraffin-embedded specimens.” Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8588, 2013. Scopus, doi:10.1117/12.2003155.
Wilson JW, Degan S, Fischer MC, Warren WS. Optical clearing and multiphoton imaging of paraffin-embedded specimens. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2013.

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

Publication Date

June 12, 2013

Volume

8588