Optical clearing and multiphoton imaging of paraffin-embedded specimens
New labeling, imaging, or analysis tools could provide new retrospective insights when applied to archived, paraffinembedded samples. Deep-tissue multiphoton microscopy of paraffin-embedded specimens is achieved using optical clearing with mineral oil. We tested a variety of murine tissue specimens including skin, lung, spleen, kidney, and heart, acquiring multiphoton autofluorescence and second-harmonic generation, and pump-probe images This technique introduces the capability for non-destructive 3-dimensional microscopic imaging of existing archived pathology specimens, enabling retrospective studies. © 2013 SPIE.