Mutations in the gene that encodes the F-actin binding protein anillin cause FSGS.

Published

Journal Article

FSGS is characterized by segmental scarring of the glomerulus and is a leading cause of kidney failure. Identification of genes causing FSGS has improved our understanding of disease mechanisms and points to defects in the glomerular epithelial cell, the podocyte, as a major factor in disease pathogenesis. Using a combination of genome-wide linkage studies and whole-exome sequencing in a kindred with familial FSGS, we identified a missense mutation R431C in anillin (ANLN), an F-actin binding cell cycle gene, as a cause of FSGS. We screened 250 additional families with FSGS and found another variant, G618C, that segregates with disease in a second family with FSGS. We demonstrate upregulation of anillin in podocytes in kidney biopsy specimens from individuals with FSGS and kidney samples from a murine model of HIV-1-associated nephropathy. Overexpression of R431C mutant ANLN in immortalized human podocytes results in enhanced podocyte motility. The mutant anillin displays reduced binding to the slit diaphragm-associated scaffold protein CD2AP. Knockdown of the ANLN gene in zebrafish morphants caused a loss of glomerular filtration barrier integrity, podocyte foot process effacement, and an edematous phenotype. Collectively, these findings suggest that anillin is important in maintaining the integrity of the podocyte actin cytoskeleton.

Full Text

Duke Authors

Cited Authors

  • Gbadegesin, RA; Hall, G; Adeyemo, A; Hanke, N; Tossidou, I; Burchette, J; Wu, G; Homstad, A; Sparks, MA; Gomez, J; Jiang, R; Alonso, A; Lavin, P; Conlon, P; Korstanje, R; Stander, MC; Shamsan, G; Barua, M; Spurney, R; Singhal, PC; Kopp, JB; Haller, H; Howell, D; Pollak, MR; Shaw, AS; Schiffer, M; Winn, MP

Published Date

  • September 2014

Published In

Volume / Issue

  • 25 / 9

Start / End Page

  • 1991 - 2002

PubMed ID

  • 24676636

Pubmed Central ID

  • 24676636

Electronic International Standard Serial Number (EISSN)

  • 1533-3450

International Standard Serial Number (ISSN)

  • 1046-6673

Digital Object Identifier (DOI)

  • 10.1681/ASN.2013090976

Language

  • eng