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WE-FG-BRA-01: Cancer Treatment Utilizing Photo-Activation of Psoralen with KV X-Rays.

Publication ,  Journal Article
Oldham, M; Yoon, S; Meng, B; Fathi, Z; Beyer, W; Adamson, J; Alcorta, D; Osada, T; Lyerly, K; Dewhirst, M; Fecci, P; Walder, H; Spector, N
Published in: Med Phys
June 2016

PURPOSE: This work investigates X-PACT (X-ray Psoralen Activated Cancer Therapy): a new approach for the treatment of cancer. X-PACT utilizes psoralen, a potent anti-cancer therapeutic with immunogenic anti-cancer potential. Psoralen therapies have been limited due to the requirement for psoralen activation by UVA light. X-PACT solves this challenge by activating psoralen with UV light emitted from novel non-tethered phosphors (co-incubated with psoralen) that absorb x-rays and reradiate (phosphoresce) at UV wavelengths. METHODS: The efficacy of X-PACT was evaluated in both in-vitro and in-vivo settings. In-vitro studies utilized breast (4T1), glioma (CT2A) and sarcoma (KP-B) cell lines. Cells were exposed to X-PACT treatments where the concentrations of drug (psoralen and phosphor) and radiation parameters (energy, dose, and dose rate) were varied. Efficacy was evaluated primarily using flow cell cytometry to investigate treatment induced apoptosis. Methylene blue staining, and WST assays were also used. X-PACT was then evaluated in an in-vivo pilot study on BALBc mice with syngeneic 4T1 tumors, including control arms for X-PACT components. Analysis focused on tumor growth delay. RESULTS: A multivariable regression analysis of 36 independent in-vitro irradiation experiments demonstrated that X-PACT induces significant tumor cell apoptosis and cytotoxicity on all three tumor cell lines in-vitro (p<0.0001). Neither psoralen nor phosphor alone had a strongly significant effect. The in-vivo studies show a pronounced tumor growth delay when compared to controls (42% reduction at 25 days, p=0.0002). CONCLUSIONS: These studies demonstrate for the first time a therapeutic effect for X-PACT, and provide a foundation and rationale for future studies. X-PACT represents a novel treatment approach in which well-tolerated low doses of x-ray radiation generate UVA light in-situ (including deep seated lesions) which in-turn photo-activates powerful anticancer therapeutics which may lead to short and long term therapeutic effect. This work was supported by Immunolight Llc.

Duke Scholars

Published In

Med Phys

DOI

EISSN

2473-4209

Publication Date

June 2016

Volume

43

Issue

6

Start / End Page

3823

Location

United States

Related Subject Headings

  • Nuclear Medicine & Medical Imaging
  • 5105 Medical and biological physics
  • 4003 Biomedical engineering
  • 1112 Oncology and Carcinogenesis
  • 0903 Biomedical Engineering
  • 0299 Other Physical Sciences
 

Citation

APA
Chicago
ICMJE
MLA
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Oldham, M., Yoon, S., Meng, B., Fathi, Z., Beyer, W., Adamson, J., … Spector, N. (2016). WE-FG-BRA-01: Cancer Treatment Utilizing Photo-Activation of Psoralen with KV X-Rays. Med Phys, 43(6), 3823. https://doi.org/10.1118/1.4957901
Oldham, M., S. Yoon, B. Meng, Z. Fathi, W. Beyer, J. Adamson, D. Alcorta, et al. “WE-FG-BRA-01: Cancer Treatment Utilizing Photo-Activation of Psoralen with KV X-Rays.Med Phys 43, no. 6 (June 2016): 3823. https://doi.org/10.1118/1.4957901.
Oldham M, Yoon S, Meng B, Fathi Z, Beyer W, Adamson J, et al. WE-FG-BRA-01: Cancer Treatment Utilizing Photo-Activation of Psoralen with KV X-Rays. Med Phys. 2016 Jun;43(6):3823.
Oldham, M., et al. “WE-FG-BRA-01: Cancer Treatment Utilizing Photo-Activation of Psoralen with KV X-Rays.Med Phys, vol. 43, no. 6, June 2016, p. 3823. Pubmed, doi:10.1118/1.4957901.
Oldham M, Yoon S, Meng B, Fathi Z, Beyer W, Adamson J, Alcorta D, Osada T, Lyerly K, Dewhirst M, Fecci P, Walder H, Spector N. WE-FG-BRA-01: Cancer Treatment Utilizing Photo-Activation of Psoralen with KV X-Rays. Med Phys. 2016 Jun;43(6):3823.

Published In

Med Phys

DOI

EISSN

2473-4209

Publication Date

June 2016

Volume

43

Issue

6

Start / End Page

3823

Location

United States

Related Subject Headings

  • Nuclear Medicine & Medical Imaging
  • 5105 Medical and biological physics
  • 4003 Biomedical engineering
  • 1112 Oncology and Carcinogenesis
  • 0903 Biomedical Engineering
  • 0299 Other Physical Sciences