Skip to main content
Journal cover image

Surface-enhanced Raman scattering molecular sentinel nanoprobes for viral infection diagnostics.

Publication ,  Journal Article
Wang, H-N; Fales, AM; Zaas, AK; Woods, CW; Burke, T; Ginsburg, GS; Vo-Dinh, T
Published in: Anal Chim Acta
July 5, 2013

In this paper, we describe a surface-enhanced Raman scattering (SERS)-based detection approach, referred to as "molecular sentinel" (MS) plasmonic nanoprobes, to detect an RNA target related to viral infection. The MS method is essentially a label-free technique incorporating the SERS effect modulation scheme associated with silver nanoparticles and Raman dye-labeled DNA hairpin probes. Hybridization with target sequences opens the hairpin and spatially separates the Raman label from the silver surface thus reducing the SERS signal of the label. Herein, we have developed a MS nanoprobe to detect the human radical S-adenosyl methionine domain containing 2 (RSAD2) RNA target as a model system for method demonstration. The human RSAD2 gene has recently emerged as a novel host-response biomarker for diagnosis of respiratory infections. Our results showed that the RSAD2 MS nanoprobes exhibits high specificity and can detect as low as 1 nM target sequences. With the use of a portable Raman spectrometer and total RNA samples, we have also demonstrated for the first time the potential of the MS nanoprobe technology for detection of host-response RNA biomarkers for infectious disease diagnostics.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Anal Chim Acta

DOI

EISSN

1873-4324

Publication Date

July 5, 2013

Volume

786

Start / End Page

153 / 158

Location

Netherlands

Related Subject Headings

  • Spectrum Analysis, Raman
  • Silver Nitrate
  • Respiratory Tract Infections
  • Proteins
  • Oxidoreductases Acting on CH-CH Group Donors
  • Nanotechnology
  • Metal Nanoparticles
  • Humans
  • DNA Probes
  • Analytical Chemistry
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wang, H.-N., Fales, A. M., Zaas, A. K., Woods, C. W., Burke, T., Ginsburg, G. S., & Vo-Dinh, T. (2013). Surface-enhanced Raman scattering molecular sentinel nanoprobes for viral infection diagnostics. Anal Chim Acta, 786, 153–158. https://doi.org/10.1016/j.aca.2013.05.017
Wang, Hsin-Neng, Andrew M. Fales, Aimee K. Zaas, Christopher W. Woods, Thomas Burke, Geoffrey S. Ginsburg, and Tuan Vo-Dinh. “Surface-enhanced Raman scattering molecular sentinel nanoprobes for viral infection diagnostics.Anal Chim Acta 786 (July 5, 2013): 153–58. https://doi.org/10.1016/j.aca.2013.05.017.
Wang H-N, Fales AM, Zaas AK, Woods CW, Burke T, Ginsburg GS, et al. Surface-enhanced Raman scattering molecular sentinel nanoprobes for viral infection diagnostics. Anal Chim Acta. 2013 Jul 5;786:153–8.
Wang, Hsin-Neng, et al. “Surface-enhanced Raman scattering molecular sentinel nanoprobes for viral infection diagnostics.Anal Chim Acta, vol. 786, July 2013, pp. 153–58. Pubmed, doi:10.1016/j.aca.2013.05.017.
Wang H-N, Fales AM, Zaas AK, Woods CW, Burke T, Ginsburg GS, Vo-Dinh T. Surface-enhanced Raman scattering molecular sentinel nanoprobes for viral infection diagnostics. Anal Chim Acta. 2013 Jul 5;786:153–158.
Journal cover image

Published In

Anal Chim Acta

DOI

EISSN

1873-4324

Publication Date

July 5, 2013

Volume

786

Start / End Page

153 / 158

Location

Netherlands

Related Subject Headings

  • Spectrum Analysis, Raman
  • Silver Nitrate
  • Respiratory Tract Infections
  • Proteins
  • Oxidoreductases Acting on CH-CH Group Donors
  • Nanotechnology
  • Metal Nanoparticles
  • Humans
  • DNA Probes
  • Analytical Chemistry