Scholars@Duke
Christine K. Payne

Christine K. Payne

Yoh Family Professor of Mechanical Engineering and Materials Science
Thomas Lord Department of Mechanical Engineering and Materials Science
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Selected Publications

Cellular and In Vivo Response to Industrial, Food Grade, and Photocatalytic TiO2 Nanoparticles.

Journal Article The journal of physical chemistry. B · September 2024 We encounter titanium dioxide nanoparticles (TiO2 NPs) throughout our daily lives in the form of food coloring, cosmetics, and industrial materials. They are used on a massive industrial scale, with over 1 million metric tons in the global market. For the ... Full text Cite

DNA corona on nanoparticles leads to an enhanced immunostimulatory effect with implications for autoimmune diseases.

Journal Article Proc Natl Acad Sci U S A · March 12, 2024 Autoimmune and inflammatory diseases are highly complex, limiting treatment and the development of new therapies. Recent work has shown that cell-free DNA bound to biological microparticles is linked to systemic lupus erythematosus, a prototypic autoimmune ... Full text Link to item Cite

House Dust Mite Extract Forms a Der p 2 Corona on Multi-Walled Carbon Nanotubes: Implications for Allergic Airway Disease.

Journal Article Environ Sci Nano · January 1, 2024 Multi-walled carbons nanotubes (MWCNTs) are used in materials for the construction, automotive, and aerospace industries. Workers and consumers are exposed to these materials via inhalation. Existing recommended exposure limits are based on MWCNT exposures ... Full text Link to item Cite

Interaction of TiO2 nanoparticles with lung fluid proteins and the resulting macrophage inflammatory response.

Journal Article Environ Sci Nano · September 1, 2023 Inhalation is a major exposure route to nanoparticles. Following inhalation, nanoparticles first interact with the lung lining fluid, a complex mixture of proteins, lipids, and mucins. We measure the concentration and composition of lung fluid proteins ads ... Full text Link to item Cite

Gold Ions Hyperpolarize Bacteria

Journal Article Bioelectricity · June 1, 2023 Background: The study of bacterial electrophysiology is important for understanding antibacterial resistance, biofilm formation, and fundamental questions of cell growth and division. These experiments require new tools to modulate the resting membrane pot ... Full text Cite

Palmitate-mediated disruption of the endoplasmic reticulum decreases intracellular vesicle motility.

Journal Article Biophysical journal · April 2023 Essential cellular processes such as metabolism, protein synthesis, and autophagy require the intracellular transport of membrane-bound vesicles. The importance of the cytoskeleton and associated molecular motors for transport is well documented. Recent re ... Full text Cite

Concentration and composition of the protein corona as a function of incubation time and serum concentration: an automated approach to the protein corona.

Journal Article Analytical and bioanalytical chemistry · October 2022 Nanoparticles in contact with proteins form a "corona" of proteins adsorbed on the nanoparticle surface. Subsequent biological responses are then mediated by the adsorbed proteins rather than the bare nanoparticles. The use of nanoparticles as nanomedicine ... Full text Cite

Transport of lysosomes decreases in the perinuclear region: Insights from changepoint analysis.

Journal Article Biophysical journal · April 2022 Lysosomes are membrane-bound organelles that serve as the endpoint for endocytosis, phagocytosis, and autophagy, degrading the molecules, pathogens, and organelles localized within them. These cellular functions require intracellular transport. We use fluo ... Full text Cite

Effect of Thioflavin T on the Elongation Rate of Bacteria

Journal Article Bioelectricity · March 1, 2022 Background: The growing field of bacterial electrophysiology examines the relationship between bacterial membrane potential and cell division, growth, sporulation, and biofilm formation. These experiments require Nernstian fluorescent dyes to monitor membr ... Full text Cite

Food-Grade TiO2 Particles Generate Intracellular Superoxide and Alter Epigenetic Modifiers in Human Lung Cells.

Journal Article Chemical research in toxicology · November 2020 Titanium dioxide (TiO2) particles are a common ingredient in food, providing the bright white color for many candies, gums, and frostings. While ingestion of these materials has been examined previously, few studies have examined the effect of t ... Full text Cite

DNA-nanoparticle interactions: Formation of a DNA corona and its effects on a protein corona.

Journal Article Biointerphases · October 1, 2020 There has been much recent interest in the protein "corona," the nonspecific adsorption of proteins on the surface of nanoparticles used in biological applications. This research investigates an analogous DNA corona. We find that particles (200 nm and 1 μm ... Full text Link to item Cite

Automation and low-cost proteomics for characterization of the protein corona: experimental methods for big data.

Journal Article Analytical and bioanalytical chemistry · September 2020 Nanoparticles used in biological settings are exposed to proteins that adsorb on the surface forming a protein corona. These adsorbed proteins dictate the subsequent cellular response. A major challenge has been predicting what proteins will adsorb on a gi ... Full text Cite

Electrical Control of Escherichia coli Growth Measured with Simultaneous Modulation and Imaging.

Journal Article Bioelectricity · September 2020 Background: The use of electricity to mediate bacterial growth is unique in providing spatial control, but requires a more detailed understanding. Methods: We use two gold wires on a glass coverslip with an overlayer of agar to ... Full text Cite

Intracellular Generation of Superoxide by TiO2 Nanoparticles Decreases Histone Deacetylase 9 (HDAC9), an Epigenetic Modifier.

Journal Article Bioconjugate chemistry · May 2020 Titanium dioxide (TiO2) nanoparticles are used on a massive scale in commercial and industrial products. Of specific concern is how the inhalation of these nanoparticles in a manufacturing setting may affect human health. We examine the cellular ... Full text Cite

A protein corona primer for physical chemists.

Journal Article The Journal of chemical physics · October 2019 Nanoparticles present in any biological environment are exposed to extracellular proteins. These proteins adsorb on the surface of the nanoparticle forming a "protein corona." These proteins control the interaction of nanoparticles with cells. The interact ... Full text Cite

Microscopy and Cell Biology: New Methods and New Questions.

Journal Article Annual review of physical chemistry · June 2019 Understanding the cellular basis of human health and disease requires the spatial resolution of microscopy and the molecular-level details provided by spectroscopy. This review highlights imaging methods at the intersection of microscopy and spectroscopy w ... Full text Cite

Advances in materials for cellular applications (Review).

Journal Article Biointerphases · February 2019 The goal of this review is to highlight materials that show exciting promise for either entirely new cellular-level applications or new approaches to long-standing biological challenges. The authors start with two more established materials, graphene and c ... Full text Cite

TiO2 nanoparticles generate superoxide and alter gene expression in human lung cells.

Journal Article RSC advances · January 2019 TiO2 nanoparticles are widely used in consumer products and industrial applications, yet little is understood regarding how the inhalation of these nanoparticles impacts long-term health. This is especially important for the occupational safety ... Full text Cite

Protein Corona in Response to Flow: Effect on Protein Concentration and Structure.

Journal Article Biophysical journal · July 2018 Nanoparticles used in cellular applications encounter free serum proteins that adsorb onto the surface of the nanoparticle, forming a protein corona. This protein layer controls the interaction of nanoparticles with cells. For nanomedicine applications, it ... Full text Cite

Cellular and In Vivo Response to Industrial, Food Grade, and Photocatalytic TiO2 Nanoparticles.

Journal Article The journal of physical chemistry. B · September 2024 We encounter titanium dioxide nanoparticles (TiO2 NPs) throughout our daily lives in the form of food coloring, cosmetics, and industrial materials. They are used on a massive industrial scale, with over 1 million metric tons in the global market. For the ... Full text Cite

DNA corona on nanoparticles leads to an enhanced immunostimulatory effect with implications for autoimmune diseases.

Journal Article Proc Natl Acad Sci U S A · March 12, 2024 Autoimmune and inflammatory diseases are highly complex, limiting treatment and the development of new therapies. Recent work has shown that cell-free DNA bound to biological microparticles is linked to systemic lupus erythematosus, a prototypic autoimmune ... Full text Link to item Cite

House Dust Mite Extract Forms a Der p 2 Corona on Multi-Walled Carbon Nanotubes: Implications for Allergic Airway Disease.

Journal Article Environ Sci Nano · January 1, 2024 Multi-walled carbons nanotubes (MWCNTs) are used in materials for the construction, automotive, and aerospace industries. Workers and consumers are exposed to these materials via inhalation. Existing recommended exposure limits are based on MWCNT exposures ... Full text Link to item Cite

Interaction of TiO2 nanoparticles with lung fluid proteins and the resulting macrophage inflammatory response.

Journal Article Environ Sci Nano · September 1, 2023 Inhalation is a major exposure route to nanoparticles. Following inhalation, nanoparticles first interact with the lung lining fluid, a complex mixture of proteins, lipids, and mucins. We measure the concentration and composition of lung fluid proteins ads ... Full text Link to item Cite

Gold Ions Hyperpolarize Bacteria

Journal Article Bioelectricity · June 1, 2023 Background: The study of bacterial electrophysiology is important for understanding antibacterial resistance, biofilm formation, and fundamental questions of cell growth and division. These experiments require new tools to modulate the resting membrane pot ... Full text Cite

Palmitate-mediated disruption of the endoplasmic reticulum decreases intracellular vesicle motility.

Journal Article Biophysical journal · April 2023 Essential cellular processes such as metabolism, protein synthesis, and autophagy require the intracellular transport of membrane-bound vesicles. The importance of the cytoskeleton and associated molecular motors for transport is well documented. Recent re ... Full text Cite

Concentration and composition of the protein corona as a function of incubation time and serum concentration: an automated approach to the protein corona.

Journal Article Analytical and bioanalytical chemistry · October 2022 Nanoparticles in contact with proteins form a "corona" of proteins adsorbed on the nanoparticle surface. Subsequent biological responses are then mediated by the adsorbed proteins rather than the bare nanoparticles. The use of nanoparticles as nanomedicine ... Full text Cite

Transport of lysosomes decreases in the perinuclear region: Insights from changepoint analysis.

Journal Article Biophysical journal · April 2022 Lysosomes are membrane-bound organelles that serve as the endpoint for endocytosis, phagocytosis, and autophagy, degrading the molecules, pathogens, and organelles localized within them. These cellular functions require intracellular transport. We use fluo ... Full text Cite

Effect of Thioflavin T on the Elongation Rate of Bacteria

Journal Article Bioelectricity · March 1, 2022 Background: The growing field of bacterial electrophysiology examines the relationship between bacterial membrane potential and cell division, growth, sporulation, and biofilm formation. These experiments require Nernstian fluorescent dyes to monitor membr ... Full text Cite

Food-Grade TiO2 Particles Generate Intracellular Superoxide and Alter Epigenetic Modifiers in Human Lung Cells.

Journal Article Chemical research in toxicology · November 2020 Titanium dioxide (TiO2) particles are a common ingredient in food, providing the bright white color for many candies, gums, and frostings. While ingestion of these materials has been examined previously, few studies have examined the effect of t ... Full text Cite

DNA-nanoparticle interactions: Formation of a DNA corona and its effects on a protein corona.

Journal Article Biointerphases · October 1, 2020 There has been much recent interest in the protein "corona," the nonspecific adsorption of proteins on the surface of nanoparticles used in biological applications. This research investigates an analogous DNA corona. We find that particles (200 nm and 1 μm ... Full text Link to item Cite

Automation and low-cost proteomics for characterization of the protein corona: experimental methods for big data.

Journal Article Analytical and bioanalytical chemistry · September 2020 Nanoparticles used in biological settings are exposed to proteins that adsorb on the surface forming a protein corona. These adsorbed proteins dictate the subsequent cellular response. A major challenge has been predicting what proteins will adsorb on a gi ... Full text Cite

Electrical Control of Escherichia coli Growth Measured with Simultaneous Modulation and Imaging.

Journal Article Bioelectricity · September 2020 Background: The use of electricity to mediate bacterial growth is unique in providing spatial control, but requires a more detailed understanding. Methods: We use two gold wires on a glass coverslip with an overlayer of agar to ... Full text Cite

Intracellular Generation of Superoxide by TiO2 Nanoparticles Decreases Histone Deacetylase 9 (HDAC9), an Epigenetic Modifier.

Journal Article Bioconjugate chemistry · May 2020 Titanium dioxide (TiO2) nanoparticles are used on a massive scale in commercial and industrial products. Of specific concern is how the inhalation of these nanoparticles in a manufacturing setting may affect human health. We examine the cellular ... Full text Cite

A protein corona primer for physical chemists.

Journal Article The Journal of chemical physics · October 2019 Nanoparticles present in any biological environment are exposed to extracellular proteins. These proteins adsorb on the surface of the nanoparticle forming a "protein corona." These proteins control the interaction of nanoparticles with cells. The interact ... Full text Cite

Microscopy and Cell Biology: New Methods and New Questions.

Journal Article Annual review of physical chemistry · June 2019 Understanding the cellular basis of human health and disease requires the spatial resolution of microscopy and the molecular-level details provided by spectroscopy. This review highlights imaging methods at the intersection of microscopy and spectroscopy w ... Full text Cite

Advances in materials for cellular applications (Review).

Journal Article Biointerphases · February 2019 The goal of this review is to highlight materials that show exciting promise for either entirely new cellular-level applications or new approaches to long-standing biological challenges. The authors start with two more established materials, graphene and c ... Full text Cite

TiO2 nanoparticles generate superoxide and alter gene expression in human lung cells.

Journal Article RSC advances · January 2019 TiO2 nanoparticles are widely used in consumer products and industrial applications, yet little is understood regarding how the inhalation of these nanoparticles impacts long-term health. This is especially important for the occupational safety ... Full text Cite

Protein Corona in Response to Flow: Effect on Protein Concentration and Structure.

Journal Article Biophysical journal · July 2018 Nanoparticles used in cellular applications encounter free serum proteins that adsorb onto the surface of the nanoparticle, forming a protein corona. This protein layer controls the interaction of nanoparticles with cells. For nanomedicine applications, it ... Full text Cite

Nanoparticle-Cell Interactions: Relevance for Public Health.

Journal Article The journal of physical chemistry. B · January 2018 Nanoparticles, especially metal oxide nanoparticles, are used in a wide range of commercial and industrial applications that result in direct human contact, such as titanium dioxide nanoparticles in paints, food colorings, and cosmetics, or indirectly thro ... Full text Cite

Modulation of action potentials using PEDOT:PSS conducting polymer microwires

Journal Article Scientific Reports · September 4, 2017 AbstractWe describe the use of PEDOT:PSS conducting polymer microwires to modulate action potentials in single cells. PEDOT:PSS conducting polymer microwires are electrochemically synthesized with diameters ranging from 860 ... Full text Open Access Cite

TiO2 Nanoparticle-Induced Oxidation of the Plasma Membrane: Importance of the Protein Corona.

Journal Article The journal of physical chemistry. B · September 2017 Titanium dioxide (TiO2) nanoparticles, used as pigments and photocatalysts, are widely present in modern society. Inhalation or ingestion of these nanoparticles can lead to cellular-level interactions. We examined the very first step in this cel ... Full text Cite

Conducting polymer nanowires for control of local protein concentration in solution

Journal Article Journal of Physics D: Applied Physics · May 4, 2017 Full text Cite

TiO2 nanoparticles alter the expression of peroxiredoxin antioxidant genes

Journal Article Journal of Physical Chemistry C · 2016 Cite

Tuning PEDOT:PSS conductivity with iron oxidants

Journal Article Organic Electronics · 2014 Cite

PEGylated nanoparticles: protein corona and secondary structure

Journal Article Proc. SPIE, Physical Chemistry of Interfaces and Nanomaterials XIII · 2014 Cite

Conditioned Media Downregulates Nuclear Expression of Nrf2

Journal Article Cellular and Molecular Bioengineering · 2013 Full text Cite

Internalization and Trafficking of Cell Surface Proteoglycans and Proteoglycan‐Binding Ligands

Journal Article Traffic · April 2007 Using multicolor live cell imaging in combination with biochemical assays, we have investigated an endocytic pathway mediated by cell surface proteoglycans, primary receptors for many cationic ligands. We have characterized this pathway ... Full text Cite

Cellular binding, motion, and internalization of synthetic gene delivery polymers

Journal Article Biochimica Et Biophysica Acta-Molecular Cell Research · 2007 Cite

Nanophotonic light sources for fluorescence spectroscopy and cellular imaging

Journal Article Angewandte Chemie-International Edition · 2005 Full text Cite