Scholars@Duke
Dawn Elizabeth Bowles

Dawn Elizabeth Bowles

Assistant Professor in Surgery
Surgery, Surgical Sciences
dawn.bowles@duke.edu
Box 2642 Med Ctr, Durham, NC 27710
421B MSRB, Durham, NC 27710

Selected Publications

Reconstitution of thymopoiesis via implantation of cryopreserved cultured thymus tissue into athymic recipients.

Journal Article Am J Transplant · November 13, 2025 Implantation of cultured allogeneic thymus tissue (CTTI) into athymic human recipients generates functional recipient-derived naïve T cells that are tolerant to the donor. Currently, CTTI is always performed with 12 to 21 days of thymus procurement to avoi ... Full text Link to item Cite

ANTXR1 blockade enhances cardiac function in preclinical models of heart failure.

Journal Article Nat Cardiovasc Res · November 2025 Heart disease, a leading cause of mortality worldwide, is in urgent need of improved therapies. Fibrosis, an accumulation of collagen-rich extracellular matrix in response to injury, is a hallmark of heart disease, but clinical agents that can interfere wi ... Full text Link to item Cite

Tumour initiated purinergic signalling promotes cardiomyocyte RBFOX1 degradation and cardiotoxicity from DNA damaging anticancer agents.

Journal Article Nat Commun · July 25, 2025 It is well established tumour cells secrete signalling factors affecting distant normal tissues. What remains unresolved is whether these factors initiate a signalling cascade rendering terminally differentiated cardiomyocytes susceptible to apoptosis, a f ... Full text Link to item Cite

A High-Fidelity Porcine Model of Orthotopic Heart Transplantation Following Donation after Circulatory Death.

Journal Article J Vis Exp · June 6, 2025 The number of advanced heart failure patients who can receive a heart transplant is limited by a shortage of suitable organ donors. In efforts to expand the donor pool, alternative donation and procurement methods have been developed, including heart trans ... Full text Link to item Cite

Current approaches to optimize the donor heart for transplantation.

Journal Article J Heart Lung Transplant · April 2025 Heart transplantation remains a critical therapy for patients with end-stage heart failure, offering incremental survival and improved quality of life. One of the key components behind the success of heart transplantation is the condition and preservation ... Full text Link to item Cite

A porcine model of acute rejection for cardiac transplantation

Conference Frontiers in Cardiovascular Medicine · January 1, 2025 Ex vivo machine perfusion has been growing in utility for preserving donor organs prior to transplantation. This modality has tremendous potential for bioengineering and conditioning organs prior to transplantation using small molecule or advanced therapeu ... Full text Cite

UBR1 Promotes Sex-Dependent ACE2 Ubiquitination in Hypertension.

Journal Article Hypertension · January 2025 BACKGROUND: Ang-II (angiotensin II) impairs the function of the antihypertensive enzyme ACE2 (angiotensin-converting enzyme 2) by promoting its internalization, ubiquitination, and degradation, thus contributing to hypertension. However, few ACE2 ubiquitin ... Full text Link to item Cite

Delivery of a Muscle-Targeted Adeno-Associated Vector Via Ex Vivo Normothermic Perfusion Is Efficient, Durable, and Safe in a Preclinical Porcine Heart Transplant Model.

Journal Article Transpl Int · 2025 Normothermic ex-vivo organ perfusion (EVP) systems not only provide a physiological environment that preserves donor organ function outside the body but may also serve as platforms for ex-vivo organ modification via gene therapy. In this study, we demonstr ... Full text Link to item Cite

Genomic variance in Yucatan pigs and detection of donor-derived cell-free DNA after heart transplantation.

Journal Article PLoS One · 2025 Acute rejection, one of the most devastating complications that can occur following organ transplantation, is caused by antigenic differences between the organ donor and the recipient. Following cardiac transplantation, an estimated 12% of patients will ex ... Full text Link to item Cite

TRIM35 Monoubiquitinates H2B in Cardiac Cells, Implications for Heart Failure.

Journal Article Circ Res · July 5, 2024 BACKGROUND: The tumor suppressor and proapoptotic transcription factor P53 is induced (and activated) in several forms of heart failure, including cardiotoxicity and dilated cardiomyopathy; however, the precise mechanism that coordinates its induction with ... Full text Link to item Cite

Comprehensive Flow Cytometric, Immunohistologic, and Molecular Assessment of Thymus Function in Rhesus Macaques.

Journal Article Immunohorizons · July 1, 2024 The critical importance of the thymus for generating new naive T cells that protect against novel infections and are tolerant to self-antigens has led to a recent revival of interest in monitoring thymic function in species other than humans and mice. Nonh ... Full text Link to item Cite

UBR1 Promotes Sex-Dependent ACE2 Ubiquitination in Hypertension.

Journal Article medRxiv · May 25, 2024 BACKGROUND: Angiotensin (Ang)-II impairs the function of the antihypertensive enzyme ACE2 by promoting its internalization, ubiquitination and degradation thus contributing to hypertension. However, few ACE2 ubiquitination partners have been identified and ... Full text Link to item Cite

Metabolomic profiling during ex situ normothermic perfusion before heart transplantation defines patterns of substrate utilization and correlates with markers of allograft injury.

Conference J Heart Lung Transplant · May 2024 BACKGROUND: Cardiac metabolism is altered in heart failure and ischemia-reperfusion injury states. We hypothesized that metabolomic profiling during ex situ normothermic perfusion before heart transplantation (HT) would lend insight into myocardial substra ... Full text Link to item Cite

Deep Learning Resolves Myovascular Dynamics in the Failing Human Heart.

Journal Article JACC Basic Transl Sci · May 2024 The adult mammalian heart harbors minute levels of cycling cardiomyocytes (CMs). Large numbers of images are needed to accurately quantify cycling events using microscopy-based methods. CardioCount is a new deep learning-based pipeline to rigorously score ... Full text Link to item Cite

Ex Vivo Gene Therapy in Organ Transplantation: Considerations and Clinical Translation.

Journal Article Hum Gene Ther · April 2024 Ex vivo machine perfusion (EVMP) is rapidly growing in utility during solid organ transplantation. This form of organ preservation is transforming how organs are allocated and expanding the definition of what is considered a suitable organ for transplantat ... Full text Link to item Cite

GRAF1 Acts as a Downstream Mediator of Parkin to Regulate Mitophagy in Cardiomyocytes.

Journal Article Cells · March 4, 2024 Cardiomyocytes rely on proper mitochondrial homeostasis to maintain contractility and achieve optimal cardiac performance. Mitochondrial homeostasis is controlled by mitochondrial fission, fusion, and mitochondrial autophagy (mitophagy). Mitophagy plays a ... Full text Link to item Cite

Proteomic and phosphoproteomic characterization of cardiovascular tissues after long term exposure to simulated space radiation.

Journal Article Front Physiol · 2024 Introduction: It may take decades to develop cardiovascular dysfunction following exposure to high doses of ionizing radiation from medical therapy or from nuclear accidents. Since astronauts may be exposed continually to a complex space radiation environm ... Full text Link to item Cite

Cardiac magnetic resonance imaging characterization of acute rejection in a porcine heterotopic heart transplantation model.

Journal Article PLoS One · 2024 Preclinical disease models are important for the advancement of therapeutics towards human clinical trials. One of the difficult tasks of developing a well-characterized model is having a reliable modality with which to trend the progression of disease. Ac ... Full text Link to item Cite

Editorial: Graft preservation.

Journal Article Front Cardiovasc Med · 2024 Full text Link to item Cite

Delivery of Therapeutics to Solid Organs Using Ex Vivo Machine Perfusion

Chapter · January 1, 2024 The use of ex vivo machine perfusion for organ preservation has significantly improved solid organ transplantation outcomes. This modality of organ preservation has increased the donor pool and extended criteria for donor organs that would have previously ... Full text Cite

GRAF1 integrates PINK1-Parkin signaling and actin dynamics to mediate cardiac mitochondrial homeostasis.

Journal Article Nat Commun · December 11, 2023 The serine/threonine kinase, PINK1, and the E3 ubiquitin ligase, Parkin, are known to facilitate LC3-dependent autophagosomal encasement and lysosomal clearance of dysfunctional mitochondria, and defects in this process contribute to a variety of cardiomet ... Full text Link to item Cite

Nedd4-2 up-regulation is associated with ACE2 ubiquitination in hypertension.

Journal Article Cardiovasc Res · September 5, 2023 AIMS: Angiotensin-converting enzyme 2 (ACE2) is a critical component of the compensatory renin-angiotensin system that is down-regulated during the development of hypertension, possibly via ubiquitination. However, little is known about the mechanisms invo ... Full text Link to item Cite

Conserved chamber-specific polyploidy maintains heart function in Drosophila.

Journal Article Development · August 15, 2023 Developmentally programmed polyploidy (whole-genome duplication) of cardiomyocytes is common across evolution. Functions of such polyploidy are essentially unknown. Here, in both Drosophila larvae and human organ donors, we reveal distinct polyploidy level ... Full text Link to item Cite

Transvenous Endomyocardial Biopsy Technique for Intra-abdominal Heterotopic Cardiac Grafts.

Journal Article J Cardiovasc Transl Res · June 2023 The porcine intra-abdominal heterotopic heart transplantation model allows for the assessment of immunologic effects on cardiac transplantation without relying on the allograft to maintain hemodynamic support for the animal. Historically, allograft functio ... Full text Link to item Cite

Ex Vivo Gene Delivery to Porcine Cardiac Allografts Using a Myocardial-Enhanced Adeno-Associated Viral Vector.

Journal Article Hum Gene Ther · April 2023 Transplantation, the gold standard intervention for organ failure, is a clinical field that is ripe for applications of gene therapy. One of the major challenges in applying gene therapy to this field is the need for a method that achieves consistent and r ... Full text Link to item Cite

Conserved Chamber-Specific Polyploidy Maintains Heart Function in Drosophila.

Journal Article bioRxiv · February 11, 2023 Developmentally programmed polyploidy (whole-genome-duplication) of cardiomyocytes is common across evolution. Functions of such polyploidy are essentially unknown. Here, we reveal roles for precise polyploidy levels in cardiac tissue. We highlight a conse ... Full text Link to item Cite

An enhancer-based gene-therapy strategy for spatiotemporal control of cargoes during tissue repair.

Journal Article Cell Stem Cell · January 5, 2023 The efficacy and safety of gene-therapy strategies for indications like tissue damage hinge on precision; yet, current methods afford little spatial or temporal control of payload delivery. Here, we find that tissue-regeneration enhancer elements (TREEs) i ... Full text Link to item Cite

Video analysis of ex vivo beating hearts during preservation on the TransMedics® organ care system.

Journal Article Front Cardiovasc Med · 2023 BACKGROUND: Reliable biomarkers for assessing the viability of the donor hearts undergoing ex vivo perfusion remain elusive. A unique feature of normothermic ex vivo perfusion on the TransMedics® Organ Care System (OCS™) is that the donor heart is maintain ... Full text Link to item Cite

Gene therapy during ex situ heart perfusion: a new frontier in cardiac regenerative medicine?

Journal Article Front Cardiovasc Med · 2023 Ex situ organ preservation by machine perfusion can improve preservation of organs for transplantation. Furthermore, machine perfusion opens up the possibilities for selective immunomodulation, creation of tolerance to ischemia-reperfusion injury and/or co ... Full text Link to item Cite

TRIM35-mediated degradation of nuclear PKM2 destabilizes GATA4/6 and induces P53 in cardiomyocytes to promote heart failure.

Journal Article Sci Transl Med · November 2, 2022 Pyruvate kinase M2 (PKM2) is a glycolytic enzyme that translocates to the nucleus to regulate transcription factors in different tissues or pathologic states. Although studied extensively in cancer, its biological role in the heart remains unresolved. PKM1 ... Full text Link to item Cite

Looking on the horizon; potential and unique approaches to developing radiation countermeasures for deep space travel.

Journal Article Life Sci Space Res (Amst) · November 2022 Future lunar missions and beyond will require new and innovative approaches to radiation countermeasures. The Translational Research Institute for Space Health (TRISH) is focused on identifying and supporting unique approaches to reduce risks to human heal ... Full text Link to item Cite

Mitochondrial oxidative stress contributes to diastolic dysfunction through impaired mitochondrial dynamics.

Journal Article Redox Biol · November 2022 Diastolic dysfunction (DD) underlies heart failure with preserved ejection fraction (HFpEF), a clinical syndrome associated with aging that is becoming more prevalent. Despite extensive clinical studies, no effective treatment exists for HFpEF. Recent find ... Full text Link to item Cite

Dual Targeting of Soluble Oligomeric and Aggregated Transthyretin with a Monoclonal Antibody Ameliorates Experimental Neuropathy.

Journal Article Biology (Basel) · October 15, 2022 ATTR amyloidosis comprises a spectrum of multiple clinical presentations, including, predominantly, neuropathy and cardiomyopathy. The common triggering pathogenic protein is misfolded transthyretin, a carrier protein that destabilizes misfolds and assembl ... Full text Link to item Cite

Transcriptional and Immune Landscape of Cardiac Sarcoidosis.

Journal Article Circ Res · September 30, 2022 BACKGROUND: Cardiac involvement is an important determinant of mortality among sarcoidosis patients. Although granulomatous inflammation is a hallmark finding in cardiac sarcoidosis, the precise immune cell populations that comprise the granuloma remain un ... Full text Link to item Cite

Late onset cardiovascular dysfunction in adult mice resulting from galactic cosmic ray exposure.

Journal Article iScience · April 15, 2022 The complex and inaccessible space radiation environment poses an unresolved risk to astronaut cardiovascular health during long-term space exploration missions. To model this risk, healthy male c57BL/6 mice aged six months (corresponding to an astronaut o ... Full text Link to item Cite

A Porcine Heterotopic Heart Transplantation Protocol for Delivery of Therapeutics to a Cardiac Allograft.

Journal Article J Vis Exp · February 14, 2022 Cardiac transplantation is the gold standard treatment for end-stage heart failure. However, it remains limited by the number of available donor hearts and complications such as primary graft dysfunction and graft rejection. The recent clinical use of an e ... Full text Link to item Cite

Targeted Delivery for Cardiac Regeneration: Comparison of Intra-coronary Infusion and Intra-myocardial Injection in Porcine Hearts.

Journal Article Front Cardiovasc Med · 2022 BACKGROUND: The optimal delivery route to enhance effectiveness of regenerative therapeutics to the human heart is poorly understood. Direct intra-myocardial (IM) injection is the gold standard, however, it is relatively invasive. We thus compared targeted ... Full text Link to item Cite

Ex Vivo Delivery of Viral Vectors by Organ Perfusion for Cardiac Transplantation Gene Therapy.

Journal Article Methods Mol Biol · 2022 Recent advances in ex vivo perfusion have enabled an extended preservation time for solid organs prior to transplantation allowing for possible resuscitation of the donor organ during the preservation period. Opportunities to provide viral vector-mediated ... Full text Link to item Cite

Proteomic profiling identifies CLEC4C expression as a novel biomarker of primary graft dysfunction after heart transplantation.

Journal Article J Heart Lung Transplant · December 2021 PURPOSE: Clinical models to identify patients at high risk of primary graft dysfunction (PGD) after heart transplantation (HT) are limited, and the underlying pathophysiology of this common post-transplant complication remains poorly understood. We sought ... Full text Link to item Cite

Fortilin inhibits p53, halts cardiomyocyte apoptosis, and protects the heart against heart failure.

Journal Article Cell Death Discov · October 23, 2021 Heart failure (HF) has reached epidemic proportions in developed countries, affecting over 20 million people worldwide. Despite modern medical and device therapies, 60-70% of HF patients still die within 5 years of diagnosis as it relentlessly progresses t ... Full text Link to item Cite

Evolution of bacteria in the human gut in response to changing environments: An invisible player in the game of health.

Journal Article Comput Struct Biotechnol J · 2021 Several factors in Western society, including widespread use of antibiotics, chronic inflammation, and loss of complex eukaryotic symbionts such as helminths, have a dramatic impact on the ecosystem of the gut, affecting the microbiota hosted there. In add ... Full text Link to item Cite

PD-1 and PD-L1 expression in cardiac transplantation.

Journal Article Cardiovasc Pathol · 2021 Programmed death-ligand 1 (PD-L1), a transmembrane protein and member of the CD28 T cell family is associated with lymphocyte activation.1 PD-L1 expression is upregulated on activated antigen presenting cells such as monocytes, myeloid and dendritic cells. ... Full text Link to item Cite

Consequences of space radiation on the brain and cardiovascular system.

Journal Article J Environ Sci Health C Toxicol Carcinog · 2021 Staying longer in outer space will inevitably increase the health risks of astronauts due to the exposures to galactic cosmic rays and solar particle events. Exposure may pose a significant hazard to space flight crews not only during the mission but also ... Full text Link to item Cite

The use of an inexpensive processing aid device (the Mouse PAD) to facilitate rodent tissue banking.

Journal Article Biotechniques · July 2020 The generation of an experimental animal model often requires considerable investment of both time and money. Typically, investigators are interested in specific organs and when experimental animals are euthanized, tissues that are not the focus of the res ... Full text Link to item Cite

Successful Gene Delivery to Cardiac Allograft with Adeno-Associated Viral Vector Using Ex Vivo Storage Perfusion Platform

Conference The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation · April 1, 2020 PURPOSE: Cardiac gene therapy remains limited by inefficient transgene delivery with currently available methods (intravenous infusion, transcatheter coronary artery infusion, direct myocardial injection). We previously demonstrated in a porcine heterotopi ... Full text Cite

NADPH Oxidase 4 Regulates Inflammation in Ischemic Heart Failure: Role of Soluble Epoxide Hydrolase.

Journal Article Antioxid Redox Signal · July 1, 2019 Aims: Oxidative stress is implicated in cardiomyocyte cell death and cardiac remodeling in the failing heart. The role of NADPH oxidase 4 (NOX4) in cardiac adaptation to pressure overload is controversial, but its function in myocardial ischemic stress has ... Full text Link to item Cite

A normothermic ex vivo organ perfusion delivery method for cardiac transplantation gene therapy.

Journal Article Sci Rep · May 29, 2019 Clinically, both percutaneous and surgical approaches to deliver viral vectors to the heart either have resulted in therapeutically inadequate levels of transgene expression or have raised safety concerns associated with extra-cardiac delivery. Recent deve ... Full text Link to item Cite

A mole rat's gut microbiota suggests selective influence of diet on microbial niche space and evolution.

Journal Article Exp Biol Med (Maywood) · April 2019 The composition of the microbiota is of critical importance for health and disease, and is receiving increased scientific and medical scrutiny. Of particular interest is the role of changing diets as a function of agriculture and, perhaps to an even greate ... Full text Link to item Cite

The nuclear receptor RORα protects against angiotensin II-induced cardiac hypertrophy and heart failure.

Journal Article Am J Physiol Heart Circ Physiol · January 1, 2019 The nuclear receptor retinoic acid-related orphan receptor-α (RORα) regulates numerous critical biological processes, including central nervous system development, lymphocyte differentiation, and lipid metabolism. RORα has been recently identified in the h ... Full text Link to item Cite

Risk factors for stroke on left ventricular assist devices.

Journal Article J Card Surg · June 2018 BACKGROUND: Patients with end-stage heart failure on left ventricular assist devices (LVADs) are predisposed to a high incidence of stroke. Preoperative factors associated with increased stroke risk are poorly understood. METHODS: We performed a single-cen ... Full text Link to item Cite

Use of Two Intracorporeal Ventricular Assist Devices As a Total Artificial Heart.

Journal Article J Vis Exp · May 11, 2018 Mechanical circulatory support (MCS) has been introduced as a viable alternative to heart transplantation primarily through the use of intracorporeal ventricular assist devices (VADs) for support of the left ventricle. However, certain clinical scenarios w ... Full text Link to item Cite

S-Nitrosylation of β-Arrestins Biases Receptor Signaling and Confers Ligand Independence.

Journal Article Mol Cell · May 3, 2018 Most G protein-coupled receptors (GPCRs) signal through both heterotrimeric G proteins and β-arrestins (βarr1 and βarr2). Although synthetic ligands can elicit biased signaling by G protein- vis-à-vis βarr-mediated transduction, endogenous mechanisms for b ... Full text Link to item Cite

Mdm2 regulates cardiac contractility by inhibiting GRK2-mediated desensitization of β-adrenergic receptor signaling.

Journal Article JCI Insight · September 7, 2017 The oncoprotein Mdm2 is a RING domain-containing E3 ubiquitin ligase that ubiquitinates G protein-coupled receptor kinase 2 (GRK2) and β-arrestin2, thereby regulating β-adrenergic receptor (βAR) signaling and endocytosis. Previous studies showed that cardi ... Full text Open Access Link to item Cite

Hypoxia decreases creatine uptake in cardiomyocytes, while creatine supplementation enhances HIF activation.

Journal Article Physiol Rep · August 2017 Creatine (Cr), phosphocreatine (PCr), and creatine kinases (CK) comprise an energy shuttle linking ATP production in mitochondria with cellular consumption sites. Myocytes cannot synthesize Cr: these cells depend on uptake across the cell membrane by a spe ... Full text Link to item Cite

Upregulation of autophagy genes and the unfolded protein response in human heart failure.

Journal Article Int J Clin Exp Med · 2017 The cellular environment of the mammalian heart constantly is challenged with environmental and intrinsic pathological insults, which affect the proper folding of proteins in heart failure. The effects of damaged or misfolded proteins on the cell can be pr ... Link to item Cite

Plasma Levels of MicroRNA-155 Are Upregulated with Long-Term Left Ventricular Assist Device Support.

Journal Article ASAIO J · 2017 Left ventricular assist device (LVAD) therapy unloads the failing heart but exposes the human body to unique pathophysiologic demands such as continuous blood flow and complete univentricular support, which are associated with increased risk of adverse cli ... Full text Link to item Cite

Microgravity induces proteomics changes involved in endoplasmic reticulum stress and mitochondrial protection.

Journal Article Sci Rep · September 27, 2016 On Earth, biological systems have evolved in response to environmental stressors, interactions dictated by physical forces that include gravity. The absence of gravity is an extreme stressor and the impact of its absence on biological systems is ill-define ... Full text Open Access Link to item Cite

Incidence and Outcomes After Stroke on Rotary Flow Ventricular Assist Device Support

Conference The Journal of Heart and Lung Transplantation · April 2016 Full text Cite

Elevated Cardiac Troponin I in Preservation Solution Is Associated With Primary Graft Dysfunction.

Journal Article J Card Fail · February 2016 BACKGROUND: Although primary graft dysfunction (PGD) is a leading cause of mortality and morbidity early post-heart transplant, relatively little is known regarding mechanisms involved in PGD development. METHODS AND RESULTS: We examined the relationship b ... Full text Link to item Cite

Prognostic Implications of Long-Chain Acylcarnitines in Heart Failure and Reversibility With Mechanical Circulatory Support.

Journal Article J Am Coll Cardiol · January 26, 2016 BACKGROUND: Heart failure (HF) is characterized by perturbations in energy homeostasis and metabolism. The reversibility and prognostic value of circulating markers associated with these changes remain unclear. OBJECTIVES: This study sought to describe the ... Full text Link to item Cite

Chronic loss of noradrenergic tone produces β-arrestin2-mediated cocaine hypersensitivity and alters cellular D2 responses in the nucleus accumbens.

Journal Article Addict Biol · January 2016 Cocaine blocks plasma membrane monoamine transporters and increases extracellular levels of dopamine (DA), norepinephrine (NE) and serotonin (5-HT). The addictive properties of cocaine are mediated primarily by DA, while NE and 5-HT play modulatory roles. ... Full text Link to item Cite

RNA-Mediated Reprogramming of Primary Adult Human Dermal Fibroblasts into c-kit(+) Cardiac Progenitor Cells.

Journal Article Stem Cells Dev · November 15, 2015 Cardiovascular disease is the leading cause of death in the United States. Heart failure is a common, costly, and potentially fatal condition that is inadequately managed by pharmaceuticals. Cardiac repair therapies are promising alternative options. A pot ... Full text Link to item Cite

An isolated working heart system for large animal models.

Journal Article J Vis Exp · June 11, 2014 Since its introduction in the late 19(th) century, the Langendorff isolated heart perfusion apparatus, and the subsequent development of the working heart model, have been invaluable tools for studying cardiovascular function and disease(1-15). Although th ... Full text Open Access Link to item Cite

Phosphoproteomic profiling of human myocardial tissues distinguishes ischemic from non-ischemic end stage heart failure.

Journal Article PLoS One · 2014 The molecular differences between ischemic (IF) and non-ischemic (NIF) heart failure are poorly defined. A better understanding of the molecular differences between these two heart failure etiologies may lead to the development of more effective heart fail ... Full text Open Access Link to item Cite

Gene therapy for the prevention of vein graft disease.

Journal Article Transl Res · April 2013 Ischemic cardiovascular disease remains the leading cause of death worldwide. Despite advances in the medical management of atherosclerosis over the past several decades, many patients require arterial revascularization to reduce mortality and alleviate is ... Full text Link to item Cite

Adeno-associated viral vectors based on serotype 3b use components of the fibroblast growth factor receptor signaling complex for efficient transduction.

Journal Article Hum Gene Ther · October 2012 Adeno-associated virus type 3b (AAV3b) has been largely ignored by gene therapists because of the inability of vectors based on this serotype to transduce target tissues efficiently. Here we describe a phenomenon unique to AAV3b in that vectors based on th ... Full text Link to item Cite

Single amino acid modification of adeno-associated virus capsid changes transduction and humoral immune profiles.

Journal Article J Virol · August 2012 Adeno-associated virus (AAV) vectors have the potential to promote long-term gene expression. Unfortunately, humoral immunity restricts patient treatment and in addition provides an obstacle to the potential option of vector readministration. In this study ... Full text Link to item Cite

X-linked inhibitor of apoptosis protein-mediated attenuation of apoptosis, using a novel cardiac-enhanced adeno-associated viral vector.

Journal Article Hum Gene Ther · June 2012 Successful amelioration of cardiac dysfunction and heart failure through gene therapy approaches will require a transgene effective at attenuating myocardial injury, and subsequent remodeling, using an efficient and safe delivery vehicle. Our laboratory ha ... Full text Link to item Cite

Phase 1 gene therapy for Duchenne muscular dystrophy using a translational optimized AAV vector.

Journal Article Mol Ther · February 2012 Efficient and widespread gene transfer is required for successful treatment of Duchenne muscular dystrophy (DMD). Here, we performed the first clinical trial using a chimeric adeno-associated virus (AAV) capsid variant (designated AAV2.5) derived from a ra ... Full text Link to item Cite

Oxygen-coupled redox regulation of the skeletal muscle ryanodine receptor-Ca2+ release channel by NADPH oxidase 4.

Journal Article Proc Natl Acad Sci U S A · September 20, 2011 Physiological sensing of O(2) tension (partial O(2) pressure, pO(2)) plays an important role in some mammalian cellular systems, but striated muscle generally is not considered to be among them. Here we describe a molecular mechanism in skeletal muscle tha ... Full text Link to item Cite

AMPK and substrate availability regulate creatine transport in cultured cardiomyocytes.

Journal Article Am J Physiol Endocrinol Metab · May 2011 Profound alterations in myocellular creatine and phosphocreatine levels are observed during human heart failure. To maintain its intracellular creatine stores, cardiomyocytes depend upon a cell membrane creatine transporter whose regulation is not clearly ... Full text Link to item Cite

Quantitative Proteomics Of The Cytokine-Modulated Nos2 Interactome In Airway Epithelium

Conference AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · January 1, 2011 Link to item Cite

Reproducibility of left atrial ablation with high-intensity focused ultrasound energy in a calf model.

Journal Article J Thorac Cardiovasc Surg · December 2010 OBJECTIVE: Achieving transmural tissue ablation might be necessary for successful treatment of atrial fibrillation. The purpose of this study was to evaluate the reproducibility of transmural left atrial ablation using a high-intensity focused ultrasound e ... Full text Link to item Cite

Osteopontin and protein kinase C regulate PDLIM2 activation and STAT1 ubiquitination in LPS-treated murine macrophages.

Journal Article J Biol Chem · November 26, 2010 The molecular pathways regulating signal transducer and activator of transcription 1 (STAT1) levels in states of inflammation are incompletely understood. The suppressor of cytokine signaling, protein inhibitor of STAT, and SHP-1/2 tyrosine phosphatases ul ... Full text Link to item Cite

Dystrophin immunity in Duchenne's muscular dystrophy.

Journal Article N Engl J Med · October 7, 2010 We report on delivery of a functional dystrophin transgene to skeletal muscle in six patients with Duchenne's muscular dystrophy. Dystrophin-specific T cells were detected after treatment, providing evidence of transgene expression even when the functional ... Full text Link to item Cite

Acquired von Willebrand syndrome in continuous-flow ventricular assist device recipients.

Journal Article Ann Thorac Surg · October 2010 BACKGROUND: Bleeding is a major cause of morbidity in recipients of continuous-flow left ventricular assist devices (CF-LVAD). A better understanding of the impact of CF-LVAD support on the hemostatic profile is necessary to establish better strategies for ... Full text Link to item Cite

Short-lived alpha-helical intermediates in the folding of beta-sheet proteins.

Journal Article Biochemistry · July 6, 2010 Several lines of evidence point strongly toward the importance of highly alpha-helical intermediates in the folding of all globular proteins, regardless of their native structure. However, experimental refolding studies demonstrate no observable alpha-heli ... Full text Open Access Link to item Cite

Adaptation in a mouse colony monoassociated with Escherichia coli K-12 for more than 1,000 days.

Journal Article Appl Environ Microbiol · July 2010 Although mice associated with a single bacterial species have been used to provide a simple model for analysis of host-bacteria relationships, bacteria have been shown to display adaptability when grown in a variety of novel environments. In this study, ch ... Full text Link to item Cite

Comparative analysis of von Willebrand factor profiles in pulsatile and continuous left ventricular assist device recipients.

Journal Article ASAIO J · 2010 A higher rate of nonsurgical bleeding has been observed in nonpulsatile left ventricular assist device (LVAD) recipients. von Willebrand factor (vWF) profiles were compared for nonpulsatile and pulsatile LVAD recipients to explore mechanisms that may contr ... Full text Link to item Cite

Endogenous S-nitrosothiols protect against myocardial injury.

Journal Article Proc Natl Acad Sci U S A · April 14, 2009 Despite substantial evidence that nitric oxide (NO) and/or endogenous S-nitrosothiols (SNOs) exert protective effects in a variety of cardiovascular diseases, the molecular details are largely unknown. Here we show that following left coronary artery ligat ... Full text Link to item Cite

Adeno-associated virus vectors: potential applications for cancer gene therapy.

Journal Article Cancer Gene Ther · December 2005 Augmenting cancer treatment by protein and gene delivery continues to gain momentum based on success in animal models. The primary hurdle of fully exploiting the arsenal of molecular targets and therapeutic transgenes continues to be efficient delivery. Ve ... Full text Link to item Cite

The genus dependovirus

Chapter · November 25, 2005 Cite

The transcapsidation of AAV serotypes

Conference MOLECULAR THERAPY · May 1, 2004 Link to item Cite

Role of Vp2 in AAV packaging

Conference MOLECULAR THERAPY · May 1, 2004 Link to item Cite

Cross-dressing the virion: the transcapsidation of adeno-associated virus serotypes functionally defines subgroups.

Journal Article J Virol · May 2004 For all adeno-associated virus (AAV) serotypes, 60 monomers of the Vp1, Vp2, and Vp3 structural proteins assemble via an unknown mechanism to form an intact capsid. In an effort to better understand the properties of the capsid monomers and their role in v ... Full text Link to item Cite

Marker rescue of adeno-associated virus (AAV) capsid mutants: a novel approach for chimeric AAV production.

Journal Article J Virol · January 2003 Marker rescue, the restoration of gene function by replacement of a defective gene with a normal one by recombination, has been utilized to produce novel adeno-associated virus (AAV) vectors. AAV serotype 2 (AAV2) clones containing wild-type terminal repea ... Full text Link to item Cite

Characterization of the trans-activation properties of equine herpesvirus 1 EICP0 protein.

Journal Article J Virol · February 2000 The EICP0 protein of equine herpesvirus 1 (EHV-1) is an early, viral regulatory protein that independently trans-activates EHV-1 immediate-early (IE), early, gamma1 late, and gamma2 late promoters. To assess whether this powerful trans-activator functions ... Full text Link to item Cite

The gamma2 late glycoprotein K promoter of equine herpesvirus 1 is differentially regulated by the IE and EICP0 proteins.

Journal Article Virology · April 10, 1999 The equine herpesvirus 1 immediate-early (IE) phosphoprotein is essential for the activation of transcription from viral early and late promoters and trans-represses its own promoter. Transient-transfection assays showed that the IE protein trans-represses ... Full text Link to item Cite

The ICP0 protein of equine herpesvirus 1 is an early protein that independently transactivates expression of all classes of viral promoters.

Journal Article J Virol · July 1997 To assess the role of the equine herpesvirus type 1 (EHV-1) ICP0 protein (EICP0) in gene regulation, a variety of molecular studies on the EICP0 gene and gene products of both the attenuated cell culture-adapted Kentucky A (KyA) strain and the Ab4p strain ... Full text Link to item Cite

Evaluation of the peak frequency ratio (PFR) measurement in the detection of internal carotid artery stenosis

Journal Article Journal of Clinical Ultrasound · March 1982 AbstractThe peak frequency ratio (PFR) between the internal carotid and common carotid artery Fast Fourier Transform Spectral Analysis patterns has been used to identify patients with internal carotid artery stenosis. To ev ... Full text Cite