Scholars@Duke
Dongfeng Chen

Dongfeng Chen

Associate Professor in Pathology
Pathology
dongfeng.chen@duke.edu
Duke Box 3712, RM116, Res Park III, 14 Circuit Dr, Durham, NC 27710
14 Circuit Drive, Research Park Building 3, Durham, NC 27710

Selected Publications

Interrogating post-transplant donor HLA-specific antibody characteristics and effector functions using clinical bead assays.

Journal Article Hum Immunol · November 2024 Single antigen bead (SAB) assays are the most common and sensitive method used to detect and monitor post-transplant donor specific HLA antibodies (DSA). However, a direct comparison across traditional and modified SAB assays to improve routine DSA monitor ... Full text Link to item Cite

Allosensitisation in NHP results in cross-reactive anti-SLA antibodies not detected by a lymphocyte-based flow cytometry crossmatch.

Journal Article HLA · July 2024 Xenotransplantation is a potential option for individuals for whom an acceptable human allograft is unavailable. Individuals with broadly reactive HLA antibodies due to prior exposure to foreign HLA are potential candidates for a clinical xenotransplant tr ... Full text Link to item Cite

Development of New Donor-Specific and Human Leukocyte Antigen Antibodies After Transfusion in Adult Lung Transplantation.

Journal Article J Cardiothorac Vasc Anesth · September 2023 OBJECTIVES: The development of new human leukocyte antigens (HLAs) and donor-specific antibodies (DSAs) in patients are associated with worse outcomes following lung transplantation. The authors aimed to examine the relationship between blood product trans ... Full text Open Access Link to item Cite

Proteomic profiling identifies CLEC4C expression as a novel biomarker of primary graft dysfunction after heart transplantation.

Journal Article J Heart Lung Transplant · December 2021 PURPOSE: Clinical models to identify patients at high risk of primary graft dysfunction (PGD) after heart transplantation (HT) are limited, and the underlying pathophysiology of this common post-transplant complication remains poorly understood. We sought ... Full text Link to item Cite

Increased Calculated Panel Reactive Antigen Is Associated With Increased Waitlist Time and Mortality in Lung Transplantation.

Journal Article Ann Thorac Surg · August 2020 BACKGROUND: Sensitized candidates with unacceptable antigens are a group that demands special attention in organ transplantation. Calculated panel reactive antigen (cPRA) is not used to modify allocation priorities in lung transplantation. The impact of cP ... Full text Link to item Cite

The impact of belatacept on third-party HLA alloantibodies in highly sensitized kidney transplant recipients.

Journal Article Am J Transplant · February 2020 Recent evidence suggests that belatacept reduces the durability of preexisting antibodies to class I and class II human leukocyte antigens (HLAs). In this case series of 163 highly sensitized kidney transplant candidates whose calculated panel-reactive ant ... Full text Link to item Cite

Daratumumab in Sensitized Kidney Transplantation: Potentials and Limitations of Experimental and Clinical Use.

Journal Article J Am Soc Nephrol · July 2019 BACKGROUND: Donor-specific antibodies are associated with increased risk of antibody-mediated rejection and decreased allograft survival. Therefore, reducing the risk of these antibodies remains a clinical need in transplantation. Plasma cells are a logica ... Full text Link to item Cite

Psychosocial Assessment of Candidates for Transplantation (PACT) Score Identifies High Risk Patients in Pediatric Renal Transplantation.

Journal Article Front Pediatr · 2019 Background: Currently, there is no standardized approach for determining psychosocial readiness in pediatric transplantation. We examined the utility of the Psychosocial Assessment of Candidates for Transplantation (PACT) to identify pediatric kidney trans ... Full text Open Access Link to item Cite

De novo belatacept in clinical vascularized composite allotransplantation.

Journal Article Am J Transplant · July 2018 Most immunosuppressive regimens used in clinical vascularized composite allotransplantation (VCA) have been calcineurin inhibitor (CNI)-based. As such, most recipients have experienced CNI-related side effects. Costimulation blockade, specifically CD28/B7 ... Full text Link to item Cite

Human Leukocyte Antigen Sensitization in Solid Organ Transplantation: A Primer on Terminology, Testing, and Clinical Significance for the Apheresis Practitioner.

Journal Article Ther Apher Dial · October 2017 The human leukocyte antigen (HLA) system is an important immunologic barrier that must be considered for successful solid organ transplantation. Formation of donor-specific HLA antibodies in solid organ transplantation is an important cause of allograft in ... Full text Open Access Link to item Cite

Impact of Repeated Mismatch Human Leukocyte Antigen on the Outcome of Lung Retransplantation

Conference The Journal of Heart and Lung Transplantation · April 2015 Full text Cite

P116

Conference Human Immunology · October 2014 Full text Cite

P101

Conference Human Immunology · October 2014 Full text Cite

Antibody desensitization therapy in highly sensitized lung transplant candidates.

Journal Article Am J Transplant · April 2014 As HLAs antibody detection technology has evolved, there is now detailed HLA antibody information available on prospective transplant recipients. Determining single antigen antibody specificity allows for a calculated panel reactive antibodies (cPRA) value ... Full text Link to item Cite

Implications for human leukocyte antigen antibodies after lung transplantation: a 10-year experience in 441 patients.

Journal Article Chest · July 2013 BACKGROUND: Long-term survival after lung transplant is limited by the development of chronic and progressive airflow obstruction, a condition known as bronchiolitis obliterans syndrome (BOS). While prior studies strongly implicate cellular rejection as a ... Full text Link to item Cite

Differential impact of inhibitory and activating Killer Ig-Like Receptors (KIR) on high-risk patients with myeloid and lymphoid malignancies undergoing reduced intensity transplantation from haploidentical related donors.

Journal Article Bone Marrow Transplant · June 2012 The impact of activating KIR (aKIR) and inhibitory KIR (iKIR) on OS, relapse-related mortality (RRM) and acute GVHD (aGVHD) was prospectively studied in 84 adults with high-risk hematologic malignancies receiving reduced intensity conditioning (RIC) T-cell ... Full text Link to item Cite

Acute allograft rejection: cellular and humoral processes.

Journal Article Clin Chest Med · June 2011 Acute cellular rejection affects greater than one-third of lung transplant recipients. Alloreactive T-lymphocytes constitute the basis of lung allograft rejection. Recent evidence supports a more complex immune response to the allograft. Interaction betwee ... Full text Link to item Cite

42 The Importance of Low-Titer Anti-HLA Antibodies in Cardiac Transplantation

Conference The Journal of Heart and Lung Transplantation · April 2011 Full text Cite

Mechanisms of tolerance to parental parathyroid tissue when combined with human allogeneic thymus transplantation.

Journal Article J Allergy Clin Immunol · October 2010 BACKGROUND: The induction of tolerance toward third-party solid organ grafts with allogeneic thymus tissue transplantation has not been previously demonstrated in human subjects. OBJECTIVE: Infants with complete DiGeorge anomaly (having neither thymus nor ... Full text Link to item Cite

Natural killer cell-enriched donor lymphocyte infusions from A 3-6/6 HLA matched family member following nonmyeloablative allogeneic stem cell transplantation.

Journal Article Biol Blood Marrow Transplant · August 2010 Infusing natural killer (NK) cells following transplantation may allow less infections and relapse with little risk of acute graft-versus-host disease (aGVHD). We delivered 51 total NK cell-enriched donor lymphocyte infusions (DLIs) to 30 patients followin ... Full text Link to item Cite

Rituximab For The Treatment Of Humoral Rejection After Human Lung Transplant

Journal Article AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · January 1, 2010 Link to item Cite

Acute rejection and humoral sensitization in lung transplant recipients.

Journal Article Proc Am Thorac Soc · January 15, 2009 Despite the recent introduction of many improved immunosuppressive agents for use in transplantation, acute rejection affects up to 55% of lung transplant recipients within the first year after transplant. Acute lung allograft rejection is defined as periv ... Full text Link to item Cite

Unusual clinical and immunologic manifestations of transplacentally acquired maternal T cells in severe combined immunodeficiency.

Journal Article J Allergy Clin Immunol · August 2007 The persistence of transplacentally transferred maternal T cells is common in infants with severe combined immunodeficiency (SCID), occurring in more than half of patients with SCID undergoing transplantation at our institution. These T cells respond poorl ... Full text Link to item Cite

The effect of natural killer cell killer Ig-like receptor alloreactivity on the outcome of bone marrow stem cell transplantation for severe combined immunodeficiency (SCID).

Journal Article J Clin Immunol · January 2007 Natural killer (NK) cell alloreactions against recipient cells in the setting of bone marrow transplantation have been associated with decreased rates of graft-versus-host disease (GVHD) and improved survival in transplant recipients with myeloid leukemia. ... Full text Link to item Cite