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Eric Leo Eisenstein

Associate Professor Emeritus in Medicine
Medicine, Cardiology
Duke Box 3865, Durham, NC 27710
2400 Pratt St, Durham, NC 27705

Selected Publications


Defining methods to improve eSource site start-up practices

Journal Article Contemporary Clinical Trials Communications · December 1, 2024 Background: eSource software that transfers patient electronic health record data into a clinical trial electronic case report form holds promise for increasing data quality while reducing data collection, monitoring and source document verification costs. ... Full text Cite

2024 AHA/ACC/ACS/ASNC/HRS/SCA/SCCT/SCMR/SVM Guideline for Perioperative Cardiovascular Management for Noncardiac Surgery: A Report of the American College of Cardiology/American Heart Association Joint Committee on Clinical Practice Guidelines.

Journal Article Circulation · November 5, 2024 AIM: The "2024 AHA/ACC/ACS/ASNC/HRS/SCA/SCCT/SCMR/SVM Guideline for Perioperative Cardiovascular Management for Noncardiac Surgery" provides recommendations to guide clinicians in the perioperative cardiovascular evaluation and management of adult patients ... Full text Open Access Link to item Cite

Comparing the Accuracy of Traditional vs. FHIR®-Based Extraction of Electronic Health Record Data for Two Clinical Trials.

Journal Article Stud Health Technol Inform · August 22, 2024 While pilots and production use of software based on the Health Level Seven (HL7®) Fast Healthcare Interoperability Resources (FHIR®) standard are increasing in clinical research, we lack consistent evaluative data on important outcomes, such as data accur ... Full text Link to item Cite

On-treatment analysis of torsemide versus furosemide for patients hospitalized for heart failure: A post-hoc analysis of TRANSFORM-HF.

Journal Article Eur J Heart Fail · July 2024 AIM: The TRANSFORM-HF trial demonstrated no significant outcome differences between torsemide and furosemide following hospitalization for heart failure (HF), but may have been impacted by non-adherence to the randomized diuretic. The current study sought ... Full text Link to item Cite

Source Data Verification (SDV) quality in clinical research: A scoping review

Journal Article Journal of Clinical and Translational Science · May 21, 2024 Introduction: The value of Source Data Verification (SDV) has been a common theme in the applied Clinical Translational Science literature. Yet, few published assessments of SDV quality exist even though they are needed to design risk-based and reduced mon ... Full text Cite

Impact of baseline kidney dysfunction on oral diuretic efficacy following hospitalization for heart failure - insights from TRANSFORM-HF.

Journal Article Eur J Heart Fail · May 2024 AIM: Among patients discharged after hospitalization for heart failure (HF), a strategy of torsemide versus furosemide showed no difference in all-cause mortality or hospitalization. Clinicians have traditionally favoured torsemide in the setting of kidney ... Full text Link to item Cite

Evaluating registry-based trial economics: Results from the STRESS clinical trial.

Journal Article Contemp Clin Trials Commun · April 2024 BACKGROUND: Registry-based trials have the potential to reduce randomized clinical trial (RCT) costs. However, observed cost differences also may be achieved through pragmatic trial designs. A systematic comparison of trial costs across different designs h ... Full text Link to item Cite

Health Care Cost Reductions with Machine Learning-Directed Evaluations during Radiation Therapy - An Economic Analysis of a Randomized Controlled Study.

Journal Article NEJM AI · April 2024 BACKGROUND: Machine learning (ML) may cost-effectively direct health care by identifying patients most likely to benefit from preventative interventions to avoid negative and expensive outcomes. System for High-Intensity Evaluation During Radiation Therapy ... Full text Link to item Cite

Torsemide Versus Furosemide After Discharge in Patients Hospitalized With Heart Failure Across the Spectrum of Ejection Fraction: Findings From TRANSFORM-HF.

Journal Article Circ Heart Fail · March 2024 BACKGROUND: The TRANSFORM-HF trial (Torsemide Comparison With Furosemide for Management of Heart Failure) found no significant difference in all-cause mortality or hospitalization among patients randomized to a strategy of torsemide versus furosemide follo ... Full text Link to item Cite

Torsemide vs Furosemide Among Patients With New-Onset vs Worsening Chronic Heart Failure: A Substudy of the TRANSFORM-HF Randomized Clinical Trial.

Journal Article JAMA Cardiol · February 1, 2024 IMPORTANCE: Differences in clinical profiles, outcomes, and diuretic treatment effects may exist between patients with de novo heart failure (HF) and worsening chronic HF (WHF). OBJECTIVES: To compare clinical characteristics and treatment outcomes of tors ... Full text Link to item Cite

Abstract 14826: Effect of Torsemide vs Furosemide After Discharge for Acute Heart Failure Across the Spectrum of Left Ventricular Ejection Fraction: Observations From Transform-HF

Conference Circulation · November 7, 2023 Introduction: TRANSFORM-HF trial showed no significant difference in mortality or hospitalization outcomes in patients randomized to torsemide (T) versus furosemide (F) following a heart failure (HF) ... Full text Cite

Abstract 14698: Effect of Torsemide vs. Furosemide Among Patients With New Onset and Worsening Heart Failure: The Transform-HF Randomized Trial

Conference Circulation · November 7, 2023 Introduction: In the TRANSFORM HF trial, among patients discharged after hospitalization for heart failure (HF), torsemide compared with furosemide did not result in a significant difference in all-ca ... Full text Cite

Effect of Torsemide Versus Furosemide on Symptoms and Quality of Life Among Patients Hospitalized for Heart Failure: The TRANSFORM-HF Randomized Clinical Trial.

Journal Article Circulation · July 11, 2023 BACKGROUND: Loop diuretics are a primary therapy for the symptomatic treatment of heart failure (HF), but whether torsemide improves patient symptoms and quality of life better than furosemide remains unknown. As prespecified secondary end points, the TRAN ... Full text Link to item Cite

Assessing clinical site readiness for electronic health record (EHR)-to-electronic data capture (EDC) automated data collection.

Journal Article Contemporary clinical trials · May 2023 BackgroundeSource software is used to automatically copy a patient's electronic health record data into a clinical study's electronic case report form. However, there is little evidence to assist sponsors in identifying the best sites for multi-ce ... Full text Cite

Effect of Torsemide vs Furosemide After Discharge on All-Cause Mortality in Patients Hospitalized With Heart Failure: The TRANSFORM-HF Randomized Clinical Trial.

Journal Article JAMA · January 17, 2023 IMPORTANCE: Although furosemide is the most commonly used loop diuretic in patients with heart failure, some studies suggest a potential benefit for torsemide. OBJECTIVE: To determine whether torsemide results in decreased mortality compared with furosemid ... Full text Link to item Cite

Collaborative Program to Evaluate Real World Data for Use in Clinical Studies and Regulatory Decision Making.

Journal Article AMIA Jt Summits Transl Sci Proc · 2023 The 21st Century Cures Act allows the US Food and Drug Administration to consider real world data (RWD) for new indications or post approval study requirements. However, there is limited guidance as to the relative quality of different RWD types. The ACE-R ... Link to item Cite

Moving from a training project to a real international project: an experience with an on-line mentorship model

Journal Article Heart, Vessels and Transplantation · January 1, 2023 The International Research Interdisciplinary School (IRIS) program is a training for young researchers with a biomedical background who are interested in acquiring the methodological knowledge and experience in preparing a study protocol for a project. The ... Full text Cite

Economic analysis of a single institutional review board data exchange standard in multisite clinical studies.

Journal Article Contemp Clin Trials · November 2022 BACKGROUND: Single Institutional Review Boards (sIRB) are not achieving the benefits envisioned by the National Institutes of Health. The recently published Health Level Seven (HL7®) Fast Healthcare Interoperability Resources (FHIR®) data exchange standard ... Full text Link to item Cite

Ascertaining Death Events in a Pragmatic Clinical Trial: Insights From the TRANSFORM-HF Trial.

Journal Article J Card Fail · October 2022 BACKGROUND: Death ascertainment can be challenging for pragmatic clinical trials that limit site follow-up activities to usual clinical care. METHODS AND RESULTS: We used blinded aggregate data from the ongoing ToRsemide comparison with furoSemide FOR Mana ... Full text Link to item Cite

Ascertaining Nonfatal Endpoints in Clinical Trials: Central Adjudication Versus Patient Insurance Claims.

Journal Article Ther Innov Regul Sci · November 2021 BACKGROUND: The 21st Century Cures Act allows the US Food and Drug Administration (FDA) to utilize real-world data (RWD) to create real-world evidence (RWE) for new indications or post approval study requirements. We compared central adjudication with two ... Full text Link to item Cite

Reply: Pragmatic Clinical Trials: The Big Picture.

Journal Article JACC Heart Fail · August 2021 Full text Link to item Cite

Evaluating Site-Level Implementations of the HL7 FHIR Standard to Support eSource Data Exchange in Clinical Research.

Journal Article Stud Health Technol Inform · May 27, 2021 Direct extraction and use of electronic health record (EHR) data is a long-term and multifaceted endeavor that includes design, development, implementation and evaluation of methods and tools for semi-automating tasks in the research data collection proces ... Full text Link to item Cite

Pragmatic Design of Randomized Clinical Trials for Heart Failure: Rationale and Design of the TRANSFORM-HF Trial.

Journal Article JACC Heart Fail · May 2021 Randomized clinical trials are the foundation of evidence-based medicine and central to practice guidelines and patient care decisions. Nonetheless, randomized trials in heart failure (HF) populations have become increasingly difficult to conduct and are f ... Full text Link to item Cite

Index and follow-up costs of endovascular abdominal aortic aneurysm repair from the Endurant Stent Graft System Post Approval Study (ENGAGE PAS).

Journal Article J Vasc Surg · September 2020 OBJECTIVE: Trials for endovascular aneurysm repair (EVAR) report lower perioperative mortality and morbidity, but also higher costs compared with open repair. However, few studies have examined the subsequent cost of follow-up evaluations and interventions ... Full text Link to item Cite

eSource-Enabled vs. Traditional Clinical Trial Data Collection Methods: A Site-Level Economic Analysis.

Journal Article Stud Health Technol Inform · June 16, 2020 Directly extracting data from site electronic health records for updating clinical trial databases (eSource) can reduce site data collection times and errors. We conducted a study to determine clinical trial characteristics that make eSource vs. traditiona ... Full text Link to item Cite

Evaluating the Coverage of the HL7 ® FHIR ® Standard to Support eSource Data Exchange Implementations for use in Multi-Site Clinical Research Studies.

Journal Article AMIA Annu Symp Proc · 2020 The direct use of EHR data in research, often referred to as 'eSource', has long-been a goal for researchers because of anticipated increases in data quality and reductions in site burden. eSource solutions should rely on data exchange standards for consis ... Link to item Cite

A review of measurement practice in studies of clinical decision support systems 1998-2017.

Journal Article J Am Med Inform Assoc · October 1, 2019 OBJECTIVE: To assess measurement practice in clinical decision support evaluation studies. MATERIALS AND METHODS: We identified empirical studies evaluating clinical decision support systems published from 1998 to 2017. We reviewed titles, abstracts, and f ... Full text Link to item Cite

International research interdisciplinary schools 2019 (IRIS 2019)

Journal Article Heart, Vessels and Transplantation · September 2, 2019 Full text Cite

Death: The Simple Clinical Trial Endpoint.

Journal Article Stud Health Technol Inform · 2019 Death, as a biological phenomenon, is well understood and a commonly employed endpoint for clinical trials. However, death identification and adjudication may be difficult for pragmatic clinical trials (PCT) that rely upon electronic health record and pati ... Link to item Cite

Evaluative Outcomes in Direct Extraction and Use of EHR Data in Clinical Trials.

Journal Article Stud Health Technol Inform · 2019 Use of electronic health record (EHR) data in clinical trials has long been a goal for researchers. However, few demonstrations and fewer evaluative studies have been published. The variability in outcome choice and measurement hinders synthesis of the ext ... Link to item Cite

Estimating Clinical Trial Bleeding Events Using Electronic Health Record Data.

Journal Article Stud Health Technol Inform · 2019 Clinical trials conducted for regulatory approval may include outcomes that are informative but not routinely collected in clinical practice. This situation can be problematic when pragmatic clinical trials (PCT) seek to use electronic health record (EHR) ... Link to item Cite

eSource for Standardized Health Information Exchange in Clinical Research: A Systematic Review.

Journal Article Stud Health Technol Inform · 2019 The availability of research and outcomes data is the primary limitation to evidence-based practice. Today, only a fraction of clinical decisions are based upon evidence derived from randomized control trials (RCTs), the gold-standard of knowledge discover ... Link to item Cite

Perspectives on Electronic Informed Consent From Patients Underrepresented in Research in the United States: A Focus Group Study.

Journal Article J Empir Res Hum Res Ethics · October 2018 Digital informed consent may better inform individuals about health research and increase participation. In the United States and elsewhere, minorities and rural populations are underrepresented in health research and may benefit from well-designed electro ... Full text Link to item Cite

Assessing the Financial Value of Patient Engagement: A Quantitative Approach from CTTI's Patient Groups and Clinical Trials Project.

Journal Article Ther Innov Regul Sci · March 2018 BACKGROUND: While patient groups, regulators, and sponsors are increasingly considering engaging with patients in the design and conduct of clinical development programs, sponsors are often reluctant to go beyond pilot programs because of uncertainty in th ... Full text Link to item Cite

A comparative effectiveness study of eSource used for data capture for a clinical research registry.

Journal Article Int J Med Inform · July 2017 OBJECTIVE: This pilot study compared eSource-enabled versus traditional manual data transcription (non-eSource methods) for the collection of clinical registry information. The primary study objective was to compare the time spent completing registry forms ... Full text Link to item Cite

Randomized Trial of Population-Based Clinical Decision Support to Facilitate Care Transitions.

Journal Article Stud Health Technol Inform · 2017 Medicaid beneficiaries in 6 North Carolina counties were randomly assigned to 1 of 3 clinical decision support (CDS) care transition strategies: (1) usual care (Control), (2) CDS messaging to patients and their medical homes (Reports), or (3) CDS messaging ... Link to item Cite

Using Medical Informatics to Improve Clinical Trial Operations.

Journal Article Stud Health Technol Inform · 2017 The continued escalation of clinical trial costs is becoming a public health concern. During the past decade, medical research funding peaked and there is growing concern that there may be insufficient resources to test many promising medical products. Rec ... Link to item Cite

How to Teach Health IT Evaluation: Recommendations for Health IT Evaluation Courses.

Conference Stud Health Technol Inform · 2017 Systematic health IT evaluation studies are needed to ensure system quality and safety and to provide the basis for evidence-based health informatics. Well-trained health informatics specialists are required to guarantee that health IT evaluation studies a ... Link to item Cite

Reply.

Journal Article Clin Gastroenterol Hepatol · March 2016 Full text Link to item Cite

The Effects of Two Workplace Weight Management Programs and Weight Loss on Health Care Utilization and Costs.

Journal Article J Occup Environ Med · February 2016 OBJECTIVES: The aim of this study was to compare the impact of two worksite weight management (WM [education] and WM+ [education plus counseling]) programs, on health care utilization and costs. Secondarily, compare the intervention groups to an observatio ... Full text Link to item Cite

Economic Outcomes of Extracorporeal Membrane Oxygenation With and Without Ambulation as a Bridge to Lung Transplantation.

Journal Article Respir Care · January 2016 BACKGROUND: An increasing number of centers are using active rehabilitation and ambulation for critically ill patients on extracorporeal membrane oxygenation (ECMO) as a bridge to lung transplantation. This investigation assessed the economic impact at a s ... Full text Link to item Cite

The Idiopathic Pulmonary Fibrosis Clinical Research Network (IPFnet): diagnostic and adjudication processes.

Journal Article Chest · October 2015 BACKGROUND: The National Heart, Lung, and Blood Institute-sponsored IPF Clinical Research Network (IPFnet) studies enrolled subjects with idiopathic pulmonary fibrosis (IPF) to evaluate drug therapies in treatment trials. An adjudication committee (AC) pro ... Full text Link to item Cite

Torsemide versus furosemide in heart failure patients: insights from Duke University Hospital.

Journal Article J Cardiovasc Pharmacol · May 2015 Furosemide has historically been the primary loop diuretic in heart failure patients despite data suggesting potential advantages with torsemide. We used the Duke Echocardiography Lab Database to investigate patients admitted with heart failure to Duke Hos ... Full text Open Access Link to item Cite

A reappraisal of loop diuretic choice in heart failure patients.

Journal Article Am Heart J · March 2015 The health and economic burden of heart failure is significant and continues to grow each year. Loop diuretics are an integral part of symptom management in heart failure. Furosemide is used disproportionately compared with other loop diuretics, and there ... Full text Link to item Cite

Steps to Health employee weight management randomized control trial: short-term follow-up results.

Journal Article J Occup Environ Med · February 2015 OBJECTIVE: To present the short-term follow-up findings of the Steps to Health study, a randomized trial to evaluate the effectiveness of two employee weight management programs offered within Duke University and the Health System. METHODS: A total of 550 ... Full text Open Access Link to item Cite

Predictors of Long-term Clinical Endpoints in Patients With Refractory Angina

Journal Article JOURNAL OF THE AMERICAN HEART ASSOCIATION · February 2015 Full text Link to item Cite

Predictors of long-term clinical endpoints in patients with refractory angina.

Journal Article J Am Heart Assoc · January 30, 2015 BACKGROUND: Clinical outcomes in patients with refractory angina (RA) are poorly characterized and variably described. Using the Duke Database for Cardiovascular Disease (DDCD), we explored characteristics that drive clinical endpoints in patients with cla ... Full text Link to item Cite

Health informatics-enabled workflow redesign and evaluation.

Journal Article Stud Health Technol Inform · 2015 Although health information technologies frequently serve as critical workflow components, we currently lack validated methods for identifying how heath informatics can support workflow redesign and for evaluating redesign results. In this study, we descri ... Link to item Cite

Examination of the treatment selection process in a multicenter observational study.

Journal Article Circ Cardiovasc Qual Outcomes · September 2014 BACKGROUND: Many multicenter clinical trials use permuted-block randomization to create balanced treatment allocations within clinical centers. Unlike randomized trials, observational studies do not control treatment allocation, and statistical models are ... Full text Link to item Cite

Impact of pediatric exclusivity on drug labeling and demonstrations of efficacy.

Journal Article Pediatrics · August 2014 BACKGROUND: Besides vaccines and otitis media medicines, most products prescribed for children have not been studied in the pediatric population. To remedy this, Congress enacted legislation in 1997, known as pediatric exclusivity (PE), which provides 6 mo ... Full text Link to item Cite

Reduced length of hospital stay in colorectal surgery after implementation of an enhanced recovery protocol.

Journal Article Anesth Analg · May 2014 BACKGROUND: Enhanced recovery after surgery (ERAS) is a multimodal approach to perioperative care that combines a range of interventions to enable early mobilization and feeding after surgery. We investigated the feasibility, clinical effectiveness, and co ... Full text Open Access Link to item Cite

Is overweight and class I obesity associated with increased health claims costs?

Journal Article Obesity (Silver Spring) · April 2014 OBJECTIVES: Evaluate the relationship between body mass index (BMI) and health claims costs over the last decade, assess the strength and nature of the relationship between BMI and costs, and identify comorbidities that may drive any increased costs. METHO ... Full text Link to item Cite

Efficacy and safety of nighttime dosing of antihypertensives: review of the literature and design of a pragmatic clinical trial.

Journal Article J Clin Hypertens (Greenwich) · February 2014 Blood pressure exhibits circadian variability, and nighttime blood pressure is one of the best predictors of cardiovascular (CV) events. Adults with hypertension who lack a nighttime dipping pattern are at particularly high risk. Several studies have found ... Full text Link to item Cite

Assessing exertional dyspnea in patients with idiopathic pulmonary fibrosis.

Journal Article Respir Med · January 2014 BACKGROUND: Dyspnea is a hallmark symptom of idiopathic pulmonary fibrosis (IPF), and dyspnea induced physical activity limitation is a prominent driver of quality of life impairment among IPF patients. METHODS: We examined response data for the 21 physica ... Full text Link to item Cite

Economic analysis of endovascular repair versus surveillance for patients with small abdominal aortic aneurysms.

Journal Article J Vasc Surg · August 2013 BACKGROUND: The Positive Impact of EndoVascular Options for Treating Aneurysms Early (PIVOTAL) trial enrolled individuals with small (4.0- to 5.0-cm diameter) abdominal aortic aneurysms (AAA) and reported no difference in rupture or aneurysm-related death ... Full text Link to item Cite

Suspected acute exacerbation of idiopathic pulmonary fibrosis as an outcome measure in clinical trials.

Journal Article Respir Res · July 13, 2013 BACKGROUND: Acute exacerbation of idiopathic pulmonary fibrosis has become an important outcome measure in clinical trials. This study aimed to explore the concept of suspected acute exacerbation as an outcome measure. METHODS: Three investigators retrospe ... Full text Link to item Cite

The steps to health employee weight management randomized control trial: rationale, design and baseline characteristics.

Journal Article Contemp Clin Trials · July 2013 BACKGROUND: The workplace can be an important setting for addressing obesity. An increasing number of employers offer weight management programs. PURPOSE: Present the design, rationale and baseline characteristics of the Steps to Health study (STH), a rand ... Full text Link to item Cite

A randomized trial of population-based clinical decision support to manage health and resource use for Medicaid beneficiaries.

Journal Article J Med Syst · February 2013 To determine whether a clinical decision support system can favorably impact the delivery of emergency department and hospital services. Randomized clinical trial of three clinical decision support delivery modalities: email messages to care managers (emai ... Full text Link to item Cite

Decision support for evidence-based pharmacotherapy detects adherence problems but does not impact medication use.

Journal Article Stud Health Technol Inform · 2013 Although evidence-based pharmacotherapies are a principal component of patient care, 30-50% of patients do not take their medications as prescribed. We conducted a randomized trial of two clinical decision support (CDS) interventions in 2219 patients: pati ... Link to item Cite

Off Dutch presses

Journal Article TLS-THE TIMES LITERARY SUPPLEMENT · 2013 Cite

Long-term outcomes of older diabetic patients after percutaneous coronary stenting in the United States: a report from the National Cardiovascular Data Registry, 2004 to 2008.

Journal Article J Am Coll Cardiol · December 4, 2012 OBJECTIVES: The purpose of this study was to characterize long-term outcomes of percutaneous coronary intervention (PCI) in elderly diabetic patients in routine practice. BACKGROUND: Although drug-eluting stent (DES) implantation in diabetic patients is co ... Full text Link to item Cite

Evaluating the impact of public health notification: Duke clopidogrel experience.

Journal Article Circ Cardiovasc Qual Outcomes · November 2012 BACKGROUND: Provider and public health interventions in the late 2006 sought to change the duration of clopidogrel use after drug-eluting stent (DES) implantation. We evaluated whether public health interventions were associated with changes in patient-rep ... Full text Link to item Cite

The UCSD shortness of breath questionnaire has longitudinal construct validity in idiopathic pulmonary fibrosis.

Journal Article Respir Med · October 2012 BACKGROUND: Idiopathic pulmonary fibrosis (IPF) is a progressive interstitial lung disease that often causes disabling dyspnea. In IPF and other lung diseases, patient-reported outcomes (PROs)-questionnaires designed to gather information from the patient' ... Full text Link to item Cite

Characteristics and long-term outcomes of percutaneous revascularization of unprotected left main coronary artery stenosis in the United States: a report from the National Cardiovascular Data Registry, 2004 to 2008.

Journal Article J Am Coll Cardiol · February 14, 2012 OBJECTIVES: This study sought to assess percutaneous coronary intervention (PCI) for unprotected left main coronary artery (ULMCA) stenosis in routine U.S. clinical practice. BACKGROUND: Percutaneous coronary intervention for ULMCA stenosis is controversia ... Full text Link to item Cite

Nonfatal myocardial infarction and long-term outcomes in coronary artery disease.

Journal Article Am Heart J · January 2012 BACKGROUND: Therapies may reduce short-term rates of nonfatal myocardial infarction (MI) without a detectable effect on mortality. We sought to estimate the long-term clinical implications of nonfatal MI occurring within the first 3 and 6 months after init ... Full text Link to item Cite

Linking the National Cardiovascular Data Registry CathPCI Registry with Medicare claims data: validation of a longitudinal cohort of elderly patients undergoing cardiac catheterization.

Journal Article Circ Cardiovasc Qual Outcomes · January 2012 BACKGROUND: The National Cardiovascular Data Registry CathPCI Registry was recently linked with longitudinal Centers for Medicare & Medicaid (CMS) claims data. The degree to which this linked cohort is representative of the overall CathPCI Registry and CMS ... Full text Link to item Cite

Population-based clinical decision support: a clinical and economic evaluation.

Journal Article Stud Health Technol Inform · 2012 Governments are investing in health information technologies (HIT) to improve care quality and reduce medical costs. However, evidence of these benefits is limited. We conducted a randomized trial of three clinical decision support (CDS) interventions in 2 ... Link to item Cite

Impact of the Patient-Reported Outcomes Management Information System (PROMIS) upon the design and operation of multi-center clinical trials: a qualitative research study.

Journal Article J Med Syst · December 2011 New technologies may be required to integrate the National Institutes of Health's Patient Reported Outcome Management Information System (PROMIS) into multi-center clinical trials. To better understand this need, we identified likely PROMIS reporting forma ... Full text Link to item Cite

Safety and efficacy of drug-eluting stents in older patients with chronic kidney disease: a report from the linked CathPCI Registry-CMS claims database.

Journal Article J Am Coll Cardiol · October 25, 2011 OBJECTIVES: The purpose of this study was to determine the safety and efficacy of drug-eluting stents (DES) compared with bare-metal stents (BMS) in older patients with chronic kidney disease (CKD). BACKGROUND: DES may be associated with late death and myo ... Full text Link to item Cite

Clinical and economic results from a randomized trial of clinical decision support in a rural health network.

Journal Article Stud Health Technol Inform · 2011 BACKGROUND: Replication studies evaluate technologies in usual use settings. METHODS: We conducted a clinical trial to determine whether reductions in clinical and economic results observed in a previous study could be replicated in a larger setting. Subje ... Link to item Cite

Economic analysis of centralized vs. decentralized electronic data capture in multi-center clinical studies.

Journal Article Stud Health Technol Inform · 2011 BACKGROUND: New data management models are emerging in multi-center clinical studies. We evaluated the incremental costs associated with decentralized vs. centralized models. METHODS: We developed clinical research network economic models to evaluate three ... Link to item Cite

Defining a framework for health information technology evaluation.

Journal Article Stud Health Technol Inform · 2011 Governments and providers are investing in health information technologies with little evidence as to their ultimate value. We present a conceptual framework that can be used by hospitals, clinics, and health care systems to evaluate their health informati ... Link to item Cite

Improving Medication Adherence in a Regional Healthcare Information Exchange using a Scalable, Claims-Driven, and Service-Oriented Approach.

Journal Article AMIA Annu Symp Proc · November 13, 2010 Evidence-based pharmacotherapy is a central aspect of optimal patient care for many chronic conditions. However, medication non-adherence frequently inhibits the attainment of optimal pharmacotherapy regimens. In this study, we designed, developed, and imp ... Link to item Cite

A controlled trial of sildenafil in advanced idiopathic pulmonary fibrosis.

Journal Article N Engl J Med · August 12, 2010 BACKGROUND: Sildenafil, a phosphodiesterase-5 inhibitor, may preferentially improve blood flow to well-ventilated regions of the lung in patients with advanced idiopathic pulmonary fibrosis, which could result in improvements in gas exchange. We tested the ... Full text Link to item Cite

Economic assessment of thrombocytopenia: CATCH Registry.

Journal Article J Med Syst · June 2010 Thrombocytopenia is associated with increased patient risk. However, the costs of this complication are not well defined. This study assessed the impact of thrombocytopenia on in-hospital costs using results from CATCH, an observational study that examined ... Full text Link to item Cite

Clopidogrel use and clinical events after drug-eluting stent implantation: findings from the HealthCore Integrated Research Database.

Journal Article Am Heart J · March 2010 BACKGROUND: Relationships between long-term use and level of dual antiplatelet therapy and outcomes after drug-eluting stent implantation are not well established. METHODS: This is a retrospective cohort study of 9,256 patients receiving drug-eluting stent ... Full text Link to item Cite

Certificate course in outcomes research.

Journal Article Med Teach · January 2010 Link to item Cite

Relationship between anemia and health care costs in heart failure.

Journal Article J Card Fail · December 2009 BACKGROUND: Anemia is associated with higher morbidity and mortality in patients with heart failure (HF), but its implications for heath care costs are not well described. METHODS AND RESULTS: We analyzed data on 1056 patients with symptomatic HF seen at D ... Full text Link to item Cite

Late safety, efficacy, and cost-effectiveness of a zotarolimus-eluting stent compared with a paclitaxel-eluting stent in patients with de novo coronary lesions: 2-year follow-up from the ENDEAVOR IV trial (Randomized, Controlled Trial of the Medtronic Endeavor Drug [ABT-578] Eluting Coronary Stent System Versus the Taxus Paclitaxel-Eluting Coronary Stent System in De Novo Native Coronary Artery Lesions).

Journal Article JACC Cardiovasc Interv · December 2009 Featured Publication OBJECTIVES: The aim of this study was to assess, after 2 years of follow-up, the safety, efficacy, and cost-effectiveness of a zotarolimus-eluting stent (ZES) compared with a paclitaxel-eluting stent (PES) in patients with native coronary lesions. BACKGROU ... Full text Link to item Cite

Long-term clinical and economic analysis of the Endeavor drug-eluting stent versus the Driver bare-metal stent: 4-year results from the ENDEAVOR II trial (Randomized Controlled Trial to Evaluate the Safety and Efficacy of the Medtronic AVE ABT-578 Eluting Driver Coronary Stent in De Novo Native Coronary Artery Lesions).

Journal Article JACC Cardiovasc Interv · December 2009 Featured Publication OBJECTIVES: This study was designed to evaluate long-term clinical and economic outcomes for subjects receiving Endeavor drug-eluting versus Driver bare-metal stents (both Medtronic CardioVascular, Santa Rosa, California). BACKGROUND: Early studies found t ... Full text Link to item Cite

Long-term clinical and economic analysis of the Endeavor zotarolimus-eluting stent versus the cypher sirolimus-eluting stent: 3-year results from the ENDEAVOR III trial (Randomized Controlled Trial of the Medtronic Endeavor Drug [ABT-578] Eluting Coronary Stent System Versus the Cypher Sirolimus-Eluting Coronary Stent System in De Novo Native Coronary Artery Lesions).

Journal Article JACC Cardiovasc Interv · December 2009 Featured Publication OBJECTIVES: The aim of this study was to evaluate clinical and economic outcomes for subjects receiving zotarolimus-eluting (ZES) (n = 323) versus sirolimus-eluting stents (SES) (n = 113) in the ENDEAVOR III (Randomized Controlled Trial of the Medtronic En ... Full text Link to item Cite

Assessing the economic attractiveness of coronary artery revascularization in chronic kidney disease patients.

Journal Article J Med Syst · August 2009 Chronic kidney disease (CKD) is associated with increased morbidity and mortality in coronary artery disease (CAD) patients. We compared the economic attractiveness of CAD revascularization procedures in patients with and without CKD. Our population includ ... Full text Link to item Cite

Clinical effectiveness of coronary stents in elderly persons: results from 262,700 Medicare patients in the American College of Cardiology-National Cardiovascular Data Registry.

Journal Article J Am Coll Cardiol · May 5, 2009 OBJECTIVES: The aim of this study was to compare outcomes in older individuals receiving drug-eluting stents (DES) and bare-metal stents (BMS). BACKGROUND: Comparative effectiveness of DES relative to BMS remains unclear. METHODS: Outcomes were evaluated i ... Full text Link to item Cite

Relationship Between Anemia Status and Health Care Costs in Patients With Symptomatic Heart Failure

Journal Article JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY · March 10, 2009 Link to item Cite

Do income level and race influence survival in patients receiving hemodialysis?

Journal Article Am J Med · February 2009 Featured Publication BACKGROUND: Residence in a lower-income area has been associated with higher mortality among patients receiving dialysis. We sought to determine whether these differences persist and whether the effect of income-area on mortality is different for African A ... Full text Link to item Cite

A randomized clinical trial of clinical decision support in a rural community health network serving lower income individuals: study design and baseline characteristics.

Journal Article Stud Health Technol Inform · 2009 Featured Publication Lower income individuals in the US frequently experience difficulties in obtaining access to needed health care services. We describe a randomized clinical trial that seeks to improve the quality of, and access to healthcare services for medically underser ... Link to item Cite

Health information technology economic evaluation

Journal Article · December 1, 2008 This chapter describes a framework for conducting economic analyses of health information technologies (HIT). It explains the basic principles of healthcare economic analyses and the relationships between the costs and effectiveness of a health interventio ... Full text Cite

DRIVE it home: making the case for prospective economic data collection in randomized clinical trials.

Journal Article Kidney Int · December 2008 Although the results of Pizzi et al. point to the potential economic attractiveness of a ferric gluconate treatment strategy, they may not be sufficient to change public policy and reimbursement practices. What is required is a large, simple trial that wil ... Full text Link to item Cite

Re-evaluating the volume-outcome relationship in hemodialysis patients.

Journal Article Health Policy · December 2008 Featured Publication OBJECTIVES: We sought to determine whether dialysis patient mortality rates are associated with differences in dialysis facility size, and whether this relationship differs among higher risk diabetic and lower-risk non-diabetic patients. METHODS: Using 186 ... Full text Link to item Cite

Coupling direct collection of health risk information from patients through kiosks with decision support for proactive care management.

Journal Article AMIA Annu Symp Proc · November 6, 2008 Data collection from patients for use in clinical decision making is foundational for medical practice. Increasingly, kiosks are being used to facilitate direct data collection from patients. However, kiosk-collected data are generally not integrated into ... Link to item Cite

The economic returns of pediatric clinical trials of antihypertensive drugs.

Journal Article Am Heart J · October 2008 BACKGROUND: Congress has authorized the United States Food and Drug Administration (FDA) to provide industry sponsors with a 6-month extension of drug marketing rights under the Pediatric Exclusivity Provision if FDA-requested pediatric drug trials are con ... Full text Link to item Cite

Incorporating Economics Evidence

Journal Article · September 22, 2008 Full text Cite

Long-term clinical outcomes following coronary stenting.

Journal Article Arch Intern Med · August 11, 2008 Featured Publication BACKGROUND: Clinical trials of drug-eluting stents (DES) vs bare metal stents (BMS) report a reduced need for target lesion revascularization with no difference in death or myocardial infarction. However, these trials selectively enrolled patients with low ... Full text Link to item Cite

Late adverse events after drug-eluting stent implantation.

Journal Article Curr Cardiol Rep · July 2008 Stents that elute antiproliferative drugs prevent restenosis after percutaneous coronary artery revascularization, reducing the need for repeat procedures. Randomized trials in low-risk patients supported initial regulatory approval for drug-eluting stents ... Full text Link to item Cite

Outcomes of second revascularization procedures after stent implantation.

Journal Article J Med Syst · April 2008 Drug-eluting stents (DES) reduce subsequent revascularization procedures. Although randomized trials have compared DES to brachytherapy and balloon angioplasty (PTCA) for in-stent restenosis, few long-term comparisons have been made to bare metal stents (B ... Full text Link to item Cite

Association between bleeding, blood transfusion, and costs among patients with non-ST-segment elevation acute coronary syndromes.

Journal Article Am Heart J · February 2008 BACKGROUND: Bleeding and blood transfusion are associated with increased morbidity and mortality among patients with non-ST-segment elevation acute coronary syndromes (NSTE ACS); however, the economic consequences of these complications are not well define ... Full text Link to item Cite

Incidence and prognostic significance of thrombocytopenia in patients treated with prolonged heparin therapy.

Journal Article Arch Intern Med · January 14, 2008 BACKGROUND: Despite widespread heparin use in clinical practice, the associated development of thrombocytopenia is an underrecognized and undertreated complication. METHODS: We analyzed data from consecutive hospitalized patients treated with heparin (unfr ... Full text Link to item Cite

Sensible approaches for reducing clinical trial costs.

Journal Article Clin Trials · 2008 Featured Publication BACKGROUND: Over the past decade, annual funding for biomedical research has more than doubled while new molecular entity approvals have declined by one third. OBJECTIVE: To assess the value of practices commonly employed in the conduct of large-scale clin ... Full text Link to item Cite

Development and evaluation of an improved methodology for assessing adherence to evidence-based drug therapy guidelines using claims data.

Journal Article AMIA Annu Symp Proc · October 11, 2007 Non-adherence to evidence-based pharmacotherapy is associated with increased morbidity and mortality. Claims data can be used to detect and intervene on such non-adherence, but existing claims-based approaches for measuring adherence to pharmacotherapy gui ... Link to item Cite

Evaluating implementation fidelity in health information technology interventions.

Journal Article AMIA Annu Symp Proc · October 11, 2007 Featured Publication Health information technology evaluators need to distinguish between intervention efficacy as assessed in the ideal circumstances of clinical trials and intervention effectiveness as assessed in the real world circumstances of actual practice. Because curr ... Link to item Cite

Proactive population health management in the context of a regional health information exchange using standards-based decision support.

Journal Article AMIA Annu Symp Proc · October 11, 2007 The clinic-based healthcare model does not deliver high quality, cost-effective care to populations of patients. Despite public perception that aggressive investment in information technology will lead to improvements in the safety and quality of healthcar ... Link to item Cite

Implementation fidelity: a framework for evaluation in the health information technology field.

Journal Article AMIA Annu Symp Proc · October 11, 2007 Health information technology evaluators need to distinguish between intervention efficacy as assessed in the ideal circumstances of clinical trials and intervention effectiveness as assessed in the real world circumstances of actual practice. We have deve ... Link to item Cite

Using inverse probability-weighted estimators in comparative effectiveness analyses with observational databases.

Journal Article Med Care · October 2007 Inverse probability-weighted estimation is a powerful tool for use with observational data. In this article, we describe how this propensity score-based method can be used to compare the effectiveness of 2 or more treatments. First, we discuss the inherent ... Full text Link to item Cite

Effect of medical management on recurrent stone formation following percutaneous nephrolithotomy.

Journal Article J Urol · May 2007 PURPOSE: Percutaneous nephrolithotomy is a commonly used procedure for treatment of large or complex renal calculi. In some instances postoperative residual stone fragments are an unavoidable result. Yet to our knowledge no study has examined the impact of ... Full text Link to item Cite

Economic return of clinical trials performed under the pediatric exclusivity program.

Journal Article JAMA · February 7, 2007 Featured Publication CONTEXT: In 1997, Congress authorized the US Food and Drug Administration (FDA) to grant 6-month extensions of marketing rights through the Pediatric Exclusivity Program if industry sponsors complete FDA-requested pediatric trials. The program has been pra ... Full text Open Access Link to item Cite

Clopidogrel use and long-term clinical outcomes after drug-eluting stent implantation.

Journal Article JAMA · January 10, 2007 Featured Publication CONTEXT: Recent studies of drug-eluting intracoronary stents suggest that current antiplatelet regimens may not be sufficient to prevent late stent thrombosis. OBJECTIVE: To assess the association between clopidogrel use and long-term clinical outcomes of ... Full text Link to item Cite

Producing systematic reviews of interventions in speech–language pathology: A framework for sustainability

Journal Article Evidence-Based Communication Assessment and Intervention · January 1, 2007 Systematic reviews in the health and social sciences are increasingly recognized as a valuable scientific tool to support decision making by clinicians, managers, administrators, and policy makers. Numerous organizations have arisen to fulfill growing dema ... Full text Cite

Economics methods in Cochrane systematic reviews of health promotion and public health related interventions.

Journal Article BMC Med Res Methodol · November 15, 2006 BACKGROUND: Provision of evidence on costs alongside evidence on the effects of interventions can enhance the relevance of systematic reviews to decision-making. However, patterns of use of economics methods alongside systematic review remain unclear. Revi ... Full text Link to item Cite

What is the economic value of digoxin therapy in congestive heart failure patients? Results from the DIG trial.

Journal Article J Card Fail · June 2006 Featured Publication BACKGROUND: The Digitalis Investigation Group (DIG) clinical train randomized 6800 congestive heart failure patients (ejection fraction > or =45%) to a daily regimen of either digoxin or placebo. At 37 months average follow-up, patients in both groups had ... Full text Link to item Cite

Conducting an economic analysis to assess the electrocardiogram's value.

Journal Article J Electrocardiol · April 2006 Featured Publication BACKGROUND AND PURPOSE: Health economic analyses seek to assess the relative value (cost vs health benefit trade-offs) of medical technologies. However, these methods have been underused in studies of the electrocardiogram (ECG). METHODS: We develop a fram ... Full text Link to item Cite

Problem-based learning of research skills.

Journal Article J Electrocardiol · January 2006 PURPOSE: To determine whether a short-term, problem-based educational intervention leads to increased research activity among health care practitioners. SUBJECTS AND METHODS: Participant's success was evaluated as a composite of 2 outcomes. These were (1) ... Full text Link to item Cite

Assessing the quality of medical information technology economic evaluations: room for improvement.

Journal Article AMIA Annu Symp Proc · 2006 Featured Publication Medical information systems are being recognized for their ability to improve patient outcomes. While standards for the economic evaluation of medical technologies were instituted in the mid-1990s, little is known about their application in medical informa ... Link to item Cite

Economic evaluation in medical information technology: why the numbers don't add up.

Journal Article AMIA Annu Symp Proc · 2006 Standards for the economic evaluation of medical technologies were instituted in the mid-1990s, yet little is known about their application in medical information technology studies. In a review of evaluation studies published between 1982 and 2002, we fou ... Link to item Cite

Local cost structures and the economics of robot assisted radical prostatectomy.

Journal Article J Urol · December 2005 PURPOSE: Robot assisted prostatectomy (RAP) is more costly than traditional radical retropubic prostatectomy (RRP) under the cost structures at certain hospitals. However, this finding may not be the case in all care settings. We investigated the sensitivi ... Full text Link to item Cite

Elevated body mass index and intermediate-term clinical outcomes after acute coronary syndromes.

Journal Article Am J Med · September 2005 Featured Publication PURPOSE: Obesity is a coronary disease risk factor, but its independent effect on clinical outcomes following acute coronary syndromes has not been quantified. We evaluated the relationship between elevated body mass index (BMI) and 30-day, 90-day, and 1-y ... Full text Link to item Cite

Decision models for assessing the cost effectiveness of drug-eluting stents.

Journal Article Expert Opin Pharmacother · June 2005 Percutaneous coronary intervention is an increasingly common treatment for many people with coronary disease. Randomised trials using antiproliferative, drug-eluting stents (DES) have shown important reductions in the need for repeat procedures after initi ... Full text Link to item Cite

Clinical and economic implications of the Multicenter Automatic Defibrillator Implantation Trial-II.

Journal Article Ann Intern Med · April 19, 2005 Featured Publication BACKGROUND: The Multicenter Automatic Defibrillator Implantation Trial (MADIT)-II demonstrated that implantable cardioverter defibrillators (ICDs) save lives when used in patients with a history of myocardial infarction (MI) and an ejection fraction of 0.3 ... Full text Link to item Cite

Economic analysis of the Assessment of the Safety and Efficacy of a New Thrombolytic Regimen (ASSENT-3) study: costs of reperfusion strategies in acute myocardial infarction.

Journal Article Am Heart J · April 2005 BACKGROUND: The ASSENT-3 study examined the safety and efficacy of 3 alternative regimens for ST-elevation acute myocardial infarction: full-dose tenecteplase (TNK-tPA) plus enoxaparin; half-dose TNK-tPA plus unfractionated heparin plus abciximab; and full ... Full text Link to item Cite

Surgeons' economic profiles: can we get the "right" answers?

Journal Article J Med Syst · April 2005 Hospitals and payers use economic profiling to evaluate physician and surgeon performance. However, there is significant variation in the data sources and analytic methods that are used. We used information from a hospital's cardiac surgery and cost accoun ... Full text Link to item Cite

Reducing the costs of phase III cardiovascular clinical trials.

Journal Article Am Heart J · March 2005 Featured Publication BACKGROUND: The pharmaceutical industry spends approximately 26.4 billion dollars annually for research and development (4.1 billion dollars in cardiovascular products). We compared pharmaceutical companies' planned resource use and costs in Phase III card ... Full text Link to item Cite

Assessing the potential economic value of health information technology interventions in a community-based health network.

Journal Article AMIA Annu Symp Proc · 2005 Featured Publication Health information professionals recognize the need to demonstrate that the benefits of health information technological (HIT) interventions outweigh their costs. However, such cost-benefit analyses are rarely conducted for HIT interventions, due in part t ... Link to item Cite

Developing a framework for conducting economic evaluations of community-based health information technology interventions.

Journal Article AMIA Annu Symp Proc · 2005 This study describes a framework for conducting economic analyses for health information technology (HIT) interventions, in the context of three interventions that are currently being implemented in a community-based health network caring for 17,779 Medica ... Link to item Cite

Economic impact of drug-eluting stents on hospital systems: a disease-state model.

Journal Article Am Heart J · March 2004 BACKGROUND: Drug-eluting intracoronary stents decrease restenosis and later revascularization. The US Department of Health and Human Services (HHS), recognizing the financial and clinical impact of this technology, recently proposed accelerated reimburseme ... Full text Link to item Cite

Cost-Effectiveness of New Diagnostic Tools and Therapies for Acute Coronary Syndromes

Chapter · January 1, 2004 INTRODUCTION Three 20th century trends have converged to create today’s technology-driven health care system [1]. First, increasing life expectancy (49.2 years in 1900 vs. 77.2 years in 2001 for the United States) means that people live long enough to deve ... Full text Cite

A problem-based approach to teaching outcomes research in family medicine residency.

Journal Article Fam Med · 2004 OBJECTIVE: This study's objective was to introduce research concepts and skills in family medicine residency through a problem-based course. METHODS: Eight third-year residents per year in 3 academic years participated. Groups of two-three were presented w ... Link to item Cite

The ABRF-MIRG'02 study: assembly state, thermodynamic, and kinetic analysis of an enzyme/inhibitor interaction.

Journal Article Journal of biomolecular techniques : JBT · December 2003 Fully characterizing the interactions involving biomolecules requires information on the assembly state, affinity, kinetics, and thermodynamics associated with complex formation. The analytical technologies often used to measure biomolecular interactions i ... Cite

Crystal structure of the YchF protein reveals binding sites for GTP and nucleic acid.

Journal Article Journal of bacteriology · July 2003 The bacterial protein encoded by the gene ychF is 1 of 11 universally conserved GTPases and the only one whose function is unknown. The crystal structure determination of YchF was sought to help with the functional assignment of the protein. The YchF prote ... Cite

Structure and mechanism of Pseudomonas aeruginosa PhzD, an isochorismatase from the phenazine biosynthetic pathway.

Journal Article Biochemistry · May 2003 PhzD from Pseudomonas aeruginosa is an isochorismatase involved in phenazine biosynthesis. Phenazines are antimicrobial compounds that provide Pseudomonas with a competitive advantage in certain environments and may be partly responsible for the persistenc ... Cite

A catalytic mechanism for D-Tyr-tRNATyr deacylase based on the crystal structure of Hemophilus influenzae HI0670.

Journal Article The Journal of biological chemistry · April 2003 D-Tyr-tRNA(Tyr) deacylase is an editing enzyme that removes d-tyrosine and other d-amino acids from charged tRNAs, thereby preventing incorrect incorporation of d-amino acids into proteins. A model for the catalytic mechanism of this enzyme is proposed bas ... Cite

Structure of the YibK methyltransferase from Haemophilus influenzae (HI0766): a cofactor bound at a site formed by a knot.

Journal Article Proteins · April 2003 The crystal structures of YibK from Haemophilus influenzae (HI0766) have been determined with and without bound cofactor product S-adenosylhomocysteine (AdoHcy) at 1.7 and 2.0 A resolution, respectively. The molecule adopts an alpha/beta fold, with a topol ... Cite

Interaction of GroEL and GroEL/GroES complexes with a nonnative subtilisin variant: a small-angle neutron scattering study.

Journal Article Journal of structural biology · March 2003 Small-angle neutron scattering and contrast variation were used to study the solution structure of GroEL and GroEL/GroES chaperonins complexed with a nonnative variant of the polypeptide substrate, subtilisin (PJ9). The subtilisin was 86% deuterated (dPJ9) ... Cite

Solution structure and functional ligand screening of HI0719, a highly conserved protein from bacteria to humans in the YjgF/YER057c/UK114 family.

Journal Article Biochemistry · January 2003 HI0719 belongs to a large family of highly conserved proteins with no definitive molecular function and is found in organisms ranging from bacteria to humans. We describe the NMR structure of HI0719, the first solution structure for a member of this family ... Cite

Results from a practice-based lipid clinic model in achieving low density lipoprotein cholesterol goals.

Journal Article N C Med J · 2003 OBJECTIVE: Lipid clinic models that combine diet and drug therapy have shown significantly better hyperlipidemia management than usual care. The objective is to demonstrate that such a model can be established and utilized in a primary care practice, repli ... Link to item Cite

Assisting functional assignment for hypothetical Heamophilus influenzae gene products through structural genomics.

Journal Article Current drug targets. Infectious disorders · December 2002 The three-dimensional structures of Haemophilus influenzae proteins whose biological functions are unknown are being determined as part of a structural genomics project to ask whether structural information can assist in assigning the functions of proteins ... Cite

The right test for the right price

Conference CIRCULATION · November 5, 2002 Link to item Cite

Crystal structure of the YjeE protein from Haemophilus influenzae: a putative Atpase involved in cell wall synthesis.

Journal Article Proteins · August 2002 A hypothetical protein encoded by the gene YjeE of Haemophilus influenzae was selected as part of a structural genomics project for X-ray analysis to assist with the functional assignment. The protein is considered essential to bacteria because the gene is ... Cite

Percutaneous transluminal septal reduction for hypertrophic obstructive cardiomyopathy: report from an international pilot study.

Journal Article J Med Syst · August 2002 Assessing the effectiveness of newer treatments for rare diseases can be challenging because of the small number of patients treated at individual centers. We enrolled patients undergoing percutaneous transluminal septal myocardial ablation (PTSMA) for hyp ... Full text Link to item Cite

Vasopeptidase inhibitor reduces inhospital costs for patients with congestive heart failure: results from the IMPRESS trial. Inhibition of Metallo Protease by BMS-186716 in a Randomized Exercise and Symptoms Study in Subjects With Heart Failure.

Journal Article Am Heart J · June 2002 Featured Publication BACKGROUND: The Inhibition of Metallo Protease by BMS-186716 in a Randomized Exercise and Symptoms Study in Subjects With Heart Failure (IMPRESS) clinical trial randomized patients with congestive heart failure to a daily regimen of either omapatrilat or l ... Full text Link to item Cite

Thermodynamics of reactions catalyzed by PABA synthase.

Journal Article Biophys Chem · April 10, 2002 Microcalorimetry and high-performance liquid chromatography (HPLC) have been used to conduct a thermodynamic investigation of reactions catalyzed by PABA synthase, the enzyme located at the first step in the shikimic acid metabolic pathway leading from cho ... Full text Link to item Cite

From structure to function: YrbI from Haemophilus influenzae (HI1679) is a phosphatase.

Journal Article Proteins · March 2002 The crystal structure of the YrbI protein from Haemophilus influenzae (HI1679) was determined at a 1.67-A resolution. The function of the protein had not been assigned previously, and it is annotated as hypothetical in sequence databases. The protein exhib ... Cite

Structure of Escherichia coli aminodeoxychorismate synthase: architectural conservation and diversity in chorismate-utilizing enzymes.

Journal Article Biochemistry · February 2002 Aminodeoxychorismate synthase is part of a heterodimeric complex that catalyzes the two-step biosynthesis of 4-amino-4-deoxychorismate, a precursor of p-aminobenzoate and folate in microorganisms. In the first step, a glutamine amidotransferase encoded by ... Cite

Obesity and long-term clinical and economic outcomes in coronary artery disease patients.

Journal Article Obes Res · February 2002 OBJECTIVE: Obesity is an important risk factor for coronary artery disease (CAD); however, its effect on acute coronary syndrome (ACS) patients' long-term clinical and economic outcomes has not been quantified. We assessed the impact of increasing body mas ... Full text Link to item Cite

Usefulness of psychosocial treatment of mental stress-induced myocardial ischemia in men.

Journal Article Am J Cardiol · January 15, 2002 This study examined the effects of exercise and stress management training on clinical outcomes and medical expenditures over a 5-year follow-up period in 94 male patients with established coronary artery disease (CAD) and evidence of ambulatory or mental ... Full text Link to item Cite

Dominant thermodynamic role of the third independent receptor binding site in the receptor-associated protein RAP.

Journal Article Biochemistry · December 2001 The 39 kDa receptor-associated protein (RAP) is a three-domain escort protein in the secretory pathway for several members of the low-density lipoprotein receptor (LDLR) family of endocytic receptors, including the LDLR-related protein (LRP). The minimal f ... Cite

Crystal structure of YecO from Haemophilus influenzae (HI0319) reveals a methyltransferase fold and a bound S-adenosylhomocysteine.

Journal Article Proteins · December 2001 The crystal structure of YecO from Haemophilus influenzae (HI0319), a protein annotated in the sequence databases as hypothetical, and that has not been assigned a function, has been determined at 2.2-A resolution. The structure reveals a fold typical of S ... Cite

Crystal structure of dephospho-coenzyme A kinase from Haemophilus influenzae.

Journal Article Journal of structural biology · November 2001 Dephospho-coenzyme A kinase catalyzes the final step in CoA biosynthesis, the phosphorylation of the 3'-hydroxyl group of ribose using ATP as a phosphate donor. The protein from Haemophilus influenzae was cloned and expressed, and its crystal structure was ... Cite

Advanced coronary artery disease: Appropriate end points for trials of novel therapies.

Journal Article Am Heart J · November 2001 BACKGROUND: The segment of patients with advanced coronary artery disease, or disease that is not amenable to conventional revascularization therapies, continues to grow. Because the natural history of these patients is less defined, the appropriate end po ... Full text Link to item Cite

Adolescent health in a globalised world: a picture of health inequalities.

Journal Article Adolescent medicine (Philadelphia, Pa.) · October 2001 More than half of the world's population of 6 billion people is under age 25 years. Of the estimated 1.2 billion adolescents worldwide (1 in every 5 people is an adolescent), about 85% live in developing countries and the remainder in the industrialised wo ... Cite

Solution structure of HI0257, a bacterial ribosome binding protein.

Journal Article Biochemistry · September 2001 A novel bacterial ribosome binding protein, protein Y (also known as YfiA), was recently shown to reside at the 30S/50S subunit interface and to stabilize the ribosomal 70S complex against dissociation at low magnesium ion concentrations. We report here th ... Cite

Assessing the clinical and economic burden of coronary artery disease: 1986-1998.

Journal Article Med Care · August 2001 Featured Publication BACKGROUND: The acute phase of coronary artery disease (CAD) is dramatic and receives much attention because of its high mortality and associated treatment cost. However, the acute phase typically resolves within 30 days whereas CAD is a chronic disease, w ... Full text Link to item Cite

Stability and global fold of the mouse prohormone convertase 1 pro-domain.

Journal Article Biochemistry · May 2001 We have purified the mouse prohormone convertase 1 (PC1) pro-domain expressed in Escherichia coli cells and demonstrated, using a number of biophysical methods, that this domain is an independent folding unit with a T(m) of 39 degrees C at a protein concen ... Cite

A method for assembling a collaborative research team from multiple disciplines and academic centers to study the relationships between ECG estimation and MRI measurement of myocardial infarct size.

Journal Article J Electrocardiol · 2001 A method has been developed for establishing a "University Without Walls" for the purpose of studying the relationship between electrocardiographic estimation and magnetic resonance imaging measurements of myocardial infarct size. The research team include ... Full text Link to item Cite

[Nutrition in adolescence]

Journal Article Jornal de pediatria. · November 2000 OBJECTIVE: To emphasize the importance of nutrition for healthy growth and development during adolescence. METHODS: 1 - National and international bibliographic review of the main anthropometric data to be used during puberty. 2 - Description of practical ... Cite

Crystal structure of YbaK protein from Haemophilus influenzae (HI1434) at 1.8 A resolution: functional implications.

Journal Article Proteins · July 2000 Structural genomics of proteins of unknown function most straightforwardly assists with assignment of biochemical activity when the new structure resembles that of proteins whose functions are known. When a new fold is revealed, the universe of known folds ... Cite

Cost effectiveness of early discharge after uncomplicated acute myocardial infarction.

Journal Article N Engl J Med · March 16, 2000 Featured Publication BACKGROUND: Reducing the length of hospitalizations can reduce short-term costs, but there are few data on the long-term clinical and economic consequences of early discharge. METHODS: Using data from the Global Utilization of Streptokinase and Tissue Plas ... Full text Link to item Cite

Evaluating the potential 'economic attractiveness' of new therapies in patients with non-ST elevation acute coronary syndrome.

Journal Article Pharmacoeconomics · March 2000 OBJECTIVE: To evaluate the relationship between how much a new cardiovascular therapy improves clinical outcomes over current therapies and how much more it can cost while still remaining 'economically attractive'. DESIGN: We developed a decision model to ... Full text Link to item Cite

Biological function made crystal clear - annotation of hypothetical proteins via structural genomics.

Journal Article Current opinion in biotechnology · February 2000 Many of the gene products of completely sequenced organisms are 'hypothetical' - they cannot be related to any previously characterized proteins - and so are of completely unknown function. Structural studies provide one means of obtaining functional infor ... Cite

Quality of life with end-stage coronary artery disease

Journal Article JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY · February 1, 2000 Link to item Cite

An economic analysis of an aggressive diagnostic strategy with single photon emission computed tomography myocardial perfusion imaging and early exercise stress testing in emergency department patients who present with chest pain but nondiagnostic electrocardiograms: Results from a randomized trial.

Journal Article Ann Emerg Med · January 2000 Featured Publication STUDY OBJECTIVE: Conventional emergency department testing strategies for patients with chest pain often do not provide unequivocal diagnosis of acute coronary syndromes. This study was conducted to determine whether the routine use of single photon emissi ... Full text Link to item Cite

Interaction of nonnative polypeptide substrates with the Escherichia coli chaperonin GroEL.

Journal Article Methods in molecular biology (Clifton, N.J.) · 2000 Cite

The correlation between presenting ST-segment depression and the final size of acute myocardial infarcts in patients with acute coronary syndromes.

Journal Article J Electrocardiol · 2000 The use of reperfusion therapy in patients with ST elevation acute coronary syndromes had been established. However, reperfusion therapy is usually considered contra-indicated in those with ST depression, despite the knowledge that regional posterior infar ... Full text Link to item Cite

The use of patient mix-adjusted control charts to compare in-hospital costs of care.

Journal Article Health Care Manag Sci · December 1999 We introduce a technique for patient mix-adjusting x charts and compared differences between unadjusted and patient mix-adjusted results. Our data came from coronary artery bypass graft (CABG) surgery patients at Baptist Medical Center, Oklahoma City, Okla ... Full text Link to item Cite

Dimerization of the Escherichia coli biotin repressor: corepressor function in protein assembly.

Journal Article Biochemistry · October 1999 The repressor of biotin biosynthesis binds to the biotin operator sequence to repress transcription initiation at the biotin biosynthetic operon. Site-specific binding of BirA to the biotin operator is allosterically regulated by binding of the small molec ... Cite

Nucleoside diphosphate kinase from bovine retina: purification, subcellular localization, molecular cloning, and three-dimensional structure.

Journal Article Biochemistry · October 1998 The biochemical and structural properties of bovine retinal nucleoside diphosphate kinase were investigated. The enzyme showed two polypeptides of approximately 17.5 and 18.5 kDa on SDS-PAGE, while isoelectric focusing revealed seven to eight proteins with ... Cite

Amino acid substitutions in the C-terminal regulatory domain disrupt allosteric effector binding to biosynthetic threonine deaminase from Escherichia coli.

Journal Article The Journal of biological chemistry · September 1998 Shifts in the sigmoidal kinetics of allosteric threonine deaminase promoted by isoleucine and valine binding control branched chain amino acid biosynthesis in Escherichia coli. A highly conserved alpha-helix in the C-terminal regulatory domain of the tetra ... Cite

Polymorphous crystallization and diffraction of threonine deaminase from Escherichia coli.

Journal Article Acta crystallographica. Section D, Biological crystallography · May 1998 The biosynthetic threonine deaminase from Escherichia coli, an allosteric tetramer with key regulatory functions, has been crystallized in several crystal forms. Two distinct forms, both belonging to either space group P3121 or P3221, with different sized ... Cite

Structure and control of pyridoxal phosphate dependent allosteric threonine deaminase.

Journal Article Structure · April 1998 BACKGROUND: Feedback inhibition of biosynthetic threonine deaminase (TD) from Escherichia coli provided one of the earliest examples of protein-based metabolic regulation. Isoleucine, the pathway end-product, and valine, the product of a parallel pathway, ... Cite

A comparison of neural network models for the prediction of the cost of care for acute coronary syndrome patients.

Journal Article Proc AMIA Symp · 1998 Acute coronary syndromes have remained the focus of many clinical economic studies due to the increasing prevalence of the disease and the tightening of cost controls. An accurate descriptive cost model for this population would be a valuable tool for clin ... Link to item Cite

Cost effectiveness of inpatient initiation of antiarrhythmic therapy for supraventricular tachycardias.

Journal Article Am J Cardiol · December 15, 1997 This study assessed the cost effectiveness of inpatient antiarrhythmic therapy initiation for supraventricular tachycardias using a metaanalysis of proarrhythmic risk and a decision analysis that compared inpatient to outpatient therapy initiation. A MEDLI ... Full text Link to item Cite

Cost implications of selective preoperative risk screening in the care of candidates for peripheral vascular operations.

Journal Article Am J Manag Care · December 1997 The preoperative identification that patients are at high risk for adverse postoperative outcomes is the first step toward preventing costly in-hospital complications. The economic implications of noninvasive screening strategies in the care of patients un ... Link to item Cite

Stable expression and rapid purification of Escherichia coli GroEL and GroES chaperonins.

Journal Article Protein expression and purification · October 1997 An Escherichia coli expression vector pRE (P. Reddy, A. Peterkofsky, and K. McKenney, 1989, Nucleic Acids Res. 17, 10473-10488), originally developed for the cloning and expression of lethal genes, was used for cloning and hyperexpression of GroEL and GroE ... Cite

Engineering the independent folding of the subtilisin BPN' prodomain: analysis of two-state folding versus protein stability.

Journal Article Biochemistry · August 1997 In complex with subtilisin BPN', the 77 amino acid prodomain folds into a stable compact structure comprising a four-stranded antiparallel beta-sheet and two three-turn alpha-helices. When isolated from subtilisin, the prodomain is 97% unfolded even under ... Cite

Chronic undernutrition during adolescence.

Journal Article Annals of the New York Academy of Sciences · May 1997 Cite

Cost-effectiveness of inpatient initiation of antiarrhythmic therapy for supraventricular tachycardias

Journal Article JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY · February 1, 1997 Link to item Cite

Assessing the value of newer pharmacologic agents in non-ST elevation patients: a decision support system application.

Journal Article Proc AMIA Annu Fall Symp · 1997 Newer pharmacologic agents have demonstrated significant clinical and economic benefit in high-risk percutaneous transluminal coronary angioplasty (PTCA) patients. However, the higher costs of these agents may prohibit their use in lower-risk coronary arte ... Link to item Cite

Assessing the Value of Newer Pharmacologic Agents in Non-ST Elevation Patients: A Decision Support System Application

Journal Article Journal of the American Medical Informatics Association · January 1, 1997 Newer pharmacologic agents have demonstrated significant clinical and economic benefit in high-risk percutaneous transluminal coronary angioplasty (PTCA) patients. However, the higher costs of these agents may prohibit their use in lower-risk coronary arte ... Cite

Redesigning the quaternary structure of R67 dihydrofolate reductase. Creation of an active monomer from a tetrameric protein by quadruplication of the gene.

Journal Article The Journal of biological chemistry · November 1996 R67 dihydrofolate reductase (DHFR) provides resistance to the antibacterial drug trimethoprim. This R-plasmid-encoded enzyme does not share any homology with chromosomal DHFR. A recent crystal structure of active, homotetrameric R67 DHFR (Narayana, N., Mat ... Cite

A mutational analysis of the binding of two different proteins to the same antibody.

Journal Article Biochemistry · July 1996 The crystal structures of the complexes between the anti-hen egg white lysozyme (HEL) antibody D1.3 and HEL and between D1.3 and the anti-D1.3 antibody E5.2 have shown that D1.3 contacts these two proteins through essentially the same set of combining site ... Cite

Nucleotide binding-promoted conformational changes release a nonnative polypeptide from the Escherichia coli chaperonin GroEL.

Journal Article Proceedings of the National Academy of Sciences of the United States of America · March 1996 The Escherichia coli chaperonins GroEL and GroES facilitate the refolding of polypeptide chains in an ATP hydrolysis-dependent reaction. The elementary steps in the binding and release of polypeptide substrates to GroEL were investigated in surface plasmon ... Cite

The effect of water activity on the association constant and the enthalpy of reaction between lysozyme and the specific antibodies D1.3 and D44.1.

Journal Article Journal of molecular recognition : JMR · 1996 The reactions of lysozyme with the specific monoclonal antibody D1.3, its Fv fragment and a mutant of the Fv, were studied under conditions of reduced water activity through the addition of the cosolutes glycerol, ethanol, dioxane and methanol. Titration c ... Cite

A primer of biostatistic and economic methods for diagnostic and prognostic modeling in nuclear cardiology: Part I.

Journal Article J Nucl Cardiol · 1996 This review presents a brief overview of existing diagnostic and prognostic methodologies to be used for the evaluation of patients undergoing noninvasive testing. In part I of this review, we will present methods for use of logistic and Cox regression ana ... Full text Link to item Cite

A comparison of three techniques for rapid model development: an application in patient risk-stratification.

Journal Article Proc AMIA Annu Fall Symp · 1996 Accurately risk-stratifying patients is a key component of health care outcomes assessment. And, many health care organizations increasingly are relying upon automated means for assistance in making patient risk-stratification decisions. Unfortunately, the ... Link to item Cite

Risk-adjusted control charts for health care assessment

Journal Article Annals of Operations Research · January 1, 1996 The recent Joint Commission on Accreditation of Healthcare Organization (JCAHO) requirement that hospital accreditation be based upon a Total Quality Management (TQM) approach has focused the attention of health care administrations on the use of technique ... Full text Cite

Superantigen binding to a T cell receptor beta chain of known three-dimensional structure.

Journal Article The Journal of experimental medicine · December 1995 The three-dimensional structure of an unglycosylated T cell antigen receptor (TCR) beta chain has recently been determined to 1.7 A resolution. To investigate whether this soluble beta chain (murine V beta 8.2J beta 2.1C beta 1) retains superantigen (SAG)- ... Cite

A monomeric variant of GroEL binds nucleotides but is inactive as a molecular chaperone.

Journal Article The Journal of biological chemistry · September 1995 The heat shock protein GroEL from Escherichia coli is a tetradecameric oligomer that facilitates the refolding of nonnative polypeptides in an ATP-hydrolysis dependent reaction. A mutant in GroEL was prepared in which lysine 3 was substituted with glutamat ... Cite

Analytical study of time series generation by feed-forward networks.

Journal Article Phys Rev Lett · September 1995 Cite

Monomer-heptamer equilibrium of the Escherichia coli chaperonin GroES.

Journal Article Biochemistry · August 1995 In an effort to clarify the role of GroES in chaperonin-facilitated protein folding, a plasmid-encoding expression system for GroES incorporating a histidine-tagged, thrombin-cleavable, N-terminal sequence was constructed. This approach facilitated the rap ... Cite

An expanded two-state model accounts for homotropic cooperativity in biosynthetic threonine deaminase from Escherichia coli.

Journal Article Biochemistry · July 1995 The linkage between substrate and regulatory effector binding to separate sites on allosteric enzymes results in shifts in their sigmoidal kinetics to regulate metabolism. Control of branched chain amino acid biosynthesis in Escherichia coli occurs in part ... Cite

Protein motion and lock and key complementarity in antigen-antibody reactions.

Journal Article Pharmaceutica acta Helvetiae · March 1995 Antibodies possess a highly complementary combining site structure to that of their specific antigens. In many instances their reactions are driven by enthalpic factors including, at least in the case of the reaction of monoclonal antibody D1.3 with lysozy ... Cite

Allosteric regulation of biosynthetic threonine deaminase from Escherichia coli: effects of isoleucine and valine on active-site ligand binding and catalysis.

Journal Article Archives of biochemistry and biophysics · January 1995 The sigmoidal steady-state kinetics of biosynthetic threonine deaminase from Escherichia coli is allosterically controlled by isoleucine and valine, the end-products of branched-chain amino acid biosynthesis. A basis for the regulation of threonine deamina ... Cite

Generation and prediction of time series by a neural network.

Journal Article Phys Rev Lett · January 1995 Cite

The hydrophobic nature of GroEL-substrate binding.

Journal Article The Journal of biological chemistry · January 1995 The molecular chaperone GroEl from Escherichia coli is a member of the highly conserved Hsp60 family of proteins that facilitates protein folding. A central question regarding the mechanism of GroEL-assisted refolding of proteins concerns its broad substra ... Cite

A clinical economics workstation for risk-adjusted health care cost management.

Journal Article Proc Annu Symp Comput Appl Med Care · 1995 This paper describes a healthcare cost accounting system which is under development at Duke University Medical Center. Our approach differs from current practice in that this system will dynamically adjust its resource usage estimates to compensate for var ... Link to item Cite

Energetics of cooperative ligand binding to the active sites of biosynthetic threonine deaminase from Escherichia coli.

Journal Article The Journal of biological chemistry · November 1994 The sigmoidal kinetics of alpha-ketobutyrate production catalyzed by threonine deaminase are shifted in the presence of the feedback inhibitor isoleucine and the activator valine to control carbon flow through branched-chain amino acid biosynthesis in Esch ... Cite

Interpolating between ferromagnets and spin glasses.

Journal Article Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics · November 1994 Cite

Cooperative binding of the feedback modifiers isoleucine and valine to biosynthetic threonine deaminase from Escherichia coli.

Journal Article The Journal of biological chemistry · November 1994 Control of the regulatory enzyme threonine deaminase from Escherichia coli is achieved by isoleucine inhibition and valine activation. The mechanism by which these heterotropic effectors regulate the enzyme was investigated by measuring the binding of isol ... Cite

Aromatic amine dehydrogenase, a second tryptophan tryptophylquinone enzyme.

Journal Article Journal of bacteriology · May 1994 Aromatic amine dehydrogenase (AADH) catalyzes the oxidative deamination of aromatic amines including tyramine and dopamine. AADH is structurally similar to methylamine dehydrogenase (MADH) and possesses the same tryptophan tryptophylquinone (TTQ) prostheti ... Cite

Equilibrium folding studies of tetrameric R67 dihydrofolate reductase.

Journal Article Biochemistry · April 1994 R67 dihydrofolate reductase (DHFR) is an R-plasmid encoded enzyme that confers resistance to the antibacterial drug trimethoprim. This enzyme is not homologous in sequence or structure to chromosomal DHFRs. Equilibrium folding of tetrameric R67 DHFR was st ... Cite

Structural and physicochemical analysis of the reaction between the anti-lysozyme antibody D1.3 and the anti-idiotopic antibodies E225 and E5.2.

Journal Article Journal of molecular recognition : JMR · March 1994 The reaction between the mouse (BALB/c) anti-idiotopic monoclonal antibodies E225 and E5.2 and idiotopes on the (BALB/c) anti-lysozyme monoclonal antibody D1.3 has been characterized by titration calorimetry, by equilibrium sedimentation and by the determi ... Cite

[Anthropometry and pediatrics]

Journal Article Jornal de pediatria · 1994 Cite

An evaluation of factors influencing Bayesian learning systems.

Journal Article J Am Med Inform Assoc · 1994 Featured Publication OBJECTIVES: To examine the influences of situational and model factors on the accuracy of Bayesian learning systems. DESIGN: This study examines the impacts of variations in two situational factors, training sample size and number of attributes, and in two ... Full text Link to item Cite

Automated monitoring of outcomes: application to treatment of drug abuse.

Journal Article Med Decis Making · 1994 This paper suggests a new approach for lowering follow-up costs, improving the delivery of health care, and monitoring treatment outcomes. An automated telephone follow-up system that calls, identifies, and interviews clients is an alternative method for m ... Full text Link to item Cite

An efficient approach to identify ilvA mutations reveals an amino-terminal catalytic domain in biosynthetic threonine deaminase from Escherichia coli.

Journal Article Journal of bacteriology · October 1993 High-level expression of the regulatory enzyme threonine deaminase in Escherichia coli strains grown on minimal medium that are deficient in the activities of enzymes needed for branched-chain amino acid biosynthesis result in growth inhibition, possibly b ... Cite

Generalization performance of complex adaptive tasks.

Journal Article Phys Rev Lett · June 1993 Cite

NIH conference. New insights into common variable immunodeficiency.

Journal Article Annals of internal medicine · May 1993 Common variable immunodeficiency (CVI) is a heterogenous immunodeficiency syndrome characterized by hypogammaglobulinemia, recurrent bacterial infections, and various immunologic abnormalities. In addition to recurrent infections, patients with this syndro ... Cite

Titration of histidine 62 in R67 dihydrofolate reductase is linked to a tetramertwo-dimer equilibrium.

Journal Article Biochemistry · February 1993 R67 dihydrofolate reductase (DHFR) is an R-plasmid encoded protein that confers clinical resistance to the antibacterial drug trimethoprim. To determine whether an acidic titration in kinetic pH profiles is related to titration of histidines 62, 162, 262, ... Cite

T-cell abnormalities in common variable immunodeficiency.

Journal Article Pediatric research · January 1993 Common variable immunodeficiency (CVI) is a heterogeneous condition marked by a number of different immunologic defects. One group of patients, perhaps 60% of the CVI group as a whole, is characterized by T cells that produce reduced amounts of IL-2 (mRNA ... Cite

Street youth: social imbalance and health risks.

Journal Article Journal of paediatrics and child health · 1993 Street youth are children and adolescents who express, through their bodies and lives, misery and social abandonment. Paradoxically, they are also the embodiment of resistance and survival strategies in a very unfavourable environment. The current deterior ... Cite

An evaluation of factors influencing Bayesian learning systems.

Journal Article Proc Annu Symp Comput Appl Med Care · 1993 This paper examines the influences of situational and model factors upon the accuracy of Bayesian learning systems. In particular, it is concerned with the impact of variations in training sample size, number of attributes, choice of Bayesian model, and cr ... Link to item Cite

Negative complementation in aspartate transcarbamylase. Analysis of hybrid enzyme molecules containing different arrangements of polypeptide chains from wild-type and inactive mutant catalytic subunits.

Journal Article The Journal of biological chemistry · November 1992 A comprehensive set of hybrid molecules of aspartate transcarbamylase (ATCase) from Escherichia coli has been constructed of wild-type and mutationally altered catalytic chains. The mutant enzymes that were virtually devoid of activity contained a replacem ... Cite

Cloning, expression, purification, and characterization of biosynthetic threonine deaminase from Escherichia coli.

Journal Article The Journal of biological chemistry · March 1991 Feedback inhibition of the regulatory enzyme threonine deaminase by isoleucine provides an important level of enzymic control over branched chain amino acid biosynthesis in Escherichia coli. Cloning ilvA, the structural gene for threonine deaminase, under ... Cite

Neural networks and the solution of nonlinear equations.

Journal Article Phys Rev Lett · July 1990 Cite

Heterotropic effectors promote a global conformational change in aspartate transcarbamoylase.

Journal Article Biochemistry · April 1990 The sigmoidal dependence of activity on substrate concentration exhibited by the regulatory enzyme aspartate transcarbamoylase (ATCase) of Escherichia coli is generally attributed to a ligand-promoted change in the quaternary structure of the enzyme. Altho ... Cite

The purification and characterization of arginase from Saccharomyces cerevisiae.

Journal Article The Journal of biological chemistry · January 1990 In Saccharomyces cerevisiae, ornithine transcarbamoylase and arginase form a regulatory multienzyme complex (Hensley, P. (1988) Curr. Top. Cell. Regul. 29, 35-75). In this complex, arginase acts as a negative allosteric effector for ornithine transcarbamoy ... Cite

Changes in stability and allosteric properties of aspartate transcarbamoylase resulting from amino acid substitutions in the zinc-binding domain of the regulatory chains.

Journal Article Proceedings of the National Academy of Sciences of the United States of America · May 1989 Changes in subunit interaction energies linked to the allosteric transition of the regulatory enzyme aspartate transcarbamoylase (ATCase; EC 2.1.3.2) from Escherichia coli are localized in part at interfaces between the six catalytic (c) and six regulatory ... Cite

Regulation of arginine metabolism in Saccharomyces cerevisiae. Association of arginase and ornithine transcarbamoylase.

Journal Article The Journal of biological chemistry · September 1986 Association of arginase and ornithine transcarbamoylase (OTCase) has been proposed to play an essential role in the regulation of arginine metabolism in Saccharomyces cerevisiae (Wiame, J.-M. (1971) Curr. Top. Cell. Reg. 4, 1-39). In this report multienzym ... Cite

The quaternary structure of ornithine transcarbamoylase and arginase from Saccharomyces cerevisiae.

Journal Article The Journal of biological chemistry · September 1986 Electron microscopic studies employing negative staining, rotary shadowing, and unidirectional shadowing have revealed the subunit architecture of ornithine transcarbamoylase and arginase from Saccharomyces cerevisiae. These techniques have confirmed the q ... Cite

Ligand binding-promoted conformational changes in yeast ornithine transcarbamoylase.

Journal Article The Journal of biological chemistry · May 1986 It has been proposed that regulatory multienzyme complex formation between yeast ornithine transcarbamoylase (OTCase) and arginase is triggered by a conformational change promoted by the binding of ornithine to a regulatory site in OTCase (Wiame, J.-M. (19 ... Cite

Purification and characterization of ornithine transcarbamoylase from Saccharomyces cerevisiae.

Journal Article The Journal of biological chemistry · April 1984 Ornithine transcarbamoylase (OTCase) has been purified in 100-mg quantities from a plasmid-containing, enzyme-overproducing strain of Saccharomyces cerevisiae. The specific activity of the homogeneous enzyme is 2.5-fold above that previously reported. The ... Cite

Evaluation of the growth hormone exercise test in normal and growth hormone-deficient children.

Journal Article Pediatrics · October 1978 A group of 168 short but otherwise normal children (group A) and 25 children deficient in growth hormone (GH) (group B) wwere studied with an exercise stimulation test to determine the expected error of this method. In group A, 125 (74.4%) had maximum GH r ... Cite