Ferdinand Kappes

Journal Article Frontiers in Drug Safety and Regulation · January 1, 2024 The use of cosmetic Botox (BoNT/A) has become increasingly prevalent among women, even during the post-pregnancy breastfeeding period. However, there is currently a limited understanding of the extent Botox enters breastmilk and its potential effect on the ... Full text Cite

DEK oncoprotein participates in heterochromatin replication via SUMO-dependent nuclear bodies.

Journal Article Journal of cell science · December 2023 The correct inheritance of chromatin structure is key for maintaining genome function and cell identity and preventing cellular transformation. DEK, a conserved non-histone chromatin protein, has recognized tumor-promoting properties, its overexpression be ... Full text Cite

DEK oncoprotein participates in heterochromatin replication via SUMO-dependent nuclear bodies

Preprint · 2023 Full text Cite

Bacterial Growth Inhibition Screen (BGIS): harnessing recombinant protein toxicity for rapid and unbiased interrogation of protein function.

Journal Article FEBS letters · May 2021 In two proof-of-concept studies, we established and validated the Bacterial Growth Inhibition Screen (BGIS), which explores recombinant protein toxicity in Escherichia coli as a largely overlooked and alternative means for basic characterization of functio ... Full text Cite

Bacterial Growth Inhibition Screen (BGIS) identifies a loss-of-function mutant of the DEK oncogene, indicating DNA modulating activities of DEK in chromatin.

Journal Article FEBS letters · May 2021 The DEK oncoprotein regulates cellular chromatin function via a number of protein-protein interactions. However, the biological relevance of its unique pseudo-SAP/SAP-box domain, which transmits DNA modulating activities in vitro, remains largely speculati ... Full text Cite

Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling.

Journal Article The Journal of clinical investigation · May 2019 The nuclear protein DEK is an endogenous DNA-binding chromatin factor regulating hematopoiesis. DEK is one of only 2 known secreted nuclear chromatin factors, but whether and how extracellular DEK regulates hematopoiesis is not known. We demonstrated that ... Full text Cite

The oncoprotein DEK affects the outcome of PARP1/2 inhibition during mild replication stress.

Journal Article PloS one · January 2019 DNA replication stress is a major source of genomic instability and is closely linked to tumor formation and progression. Poly(ADP-ribose)polymerases1/2 (PARP1/2) enzymes are activated in response to replication stress resulting in poly(ADP-ribose) (PAR) s ... Full text Open Access Cite

The oncoprotein DEK affects the outcome of PARP1/2 inhibition during replication stress

Preprint · 2019 Full text Cite

The unique DEK oncoprotein in women's health: A potential novel biomarker.

Journal Article Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · October 2018 Breast and cervical cancer are the first and fourth cancer types with the highest prevalence in women, respectively. The developmental profiles of cancer in women can vary by genetic markers and cellular events. In turn, age and lifestyle influence in the ... Full text Cite

Nucleolar-nucleoplasmic shuttling of TARG1 and its control by DNA damage-induced poly-ADP-ribosylation and by nucleolar transcription.

Journal Article Scientific reports · April 2018 Macrodomains are conserved protein folds associated with ADP-ribose binding and turnover. ADP-ribosylation is a posttranslational modification catalyzed primarily by ARTD (aka PARP) enzymes in cells. ARTDs transfer either single or multiple ADP-ribose unit ... Full text Cite

The nuclear DEK interactome supports multi-functionality.

Journal Article Proteins · January 2018 DEK is an oncoprotein that is overexpressed in many forms of cancer and participates in numerous cellular pathways. Of these different pathways, relevant interacting partners and functions of DEK are well described in regard to the regulation of chromatin ... Full text Cite

Systems analysis identifies melanoma-enriched pro-oncogenic networks controlled by the RNA binding protein CELF1.

Journal Article Nature communications · December 2017 Melanomas are well-known for their altered mRNA expression profiles. Yet, the specific contribution of mRNA binding proteins (mRBPs) to melanoma development remains unclear. Here we identify a cluster of melanoma-enriched genes under the control of CUGBP E ... Full text Cite

DEK is required for homologous recombination repair of DNA breaks.

Journal Article Scientific reports · March 2017 DEK is a highly conserved chromatin-bound protein whose upregulation across cancer types correlates with genotoxic therapy resistance. Loss of DEK induces genome instability and sensitizes cells to DNA double strand breaks (DSBs), suggesting defects in DNA ... Full text Open Access Cite

The human oncoprotein and chromatin architectural factor DEK counteracts DNA replication stress.

Journal Article Oncogene · August 2015 DNA replication stress is a major source of DNA strand breaks and genomic instability, and a hallmark of precancerous lesions. In these hyperproliferative tissues, activation of the DNA damage response results in apoptosis or senescence preventing or delay ... Full text Cite

DEK over-expression promotes mitotic defects and micronucleus formation.

Journal Article Cell cycle (Georgetown, Tex.) · January 2015 The DEK gene encodes a nuclear protein that binds chromatin and is involved in various fundamental nuclear processes including transcription, RNA splicing, DNA replication and DNA repair. Several cancer types characteristically over-express DEK at the earl ... Full text Cite

DEK oncogene expression during normal hematopoiesis and in Acute Myeloid Leukemia (AML).

Journal Article Blood cells, molecules & diseases · January 2015 DEK is important in regulating cellular processes including proliferation, differentiation and maintenance of stem cell phenotype. The translocation t(6;9) in Acute Myeloid Leukemia (AML), which fuses DEK with NUP214, confers a poor prognosis and a higher ... Full text Cite

Caspase-8-mediated PAR-4 cleavage is required for TNFα-induced apoptosis.

Journal Article Oncotarget · May 2014 The tumor suppressor protein prostate apoptosis response-4 (PAR-4) is silenced in a subset of human cancers and its down-regulation serves as a mechanism for cancer cell survival following chemotherapy. PAR-4 re-expression selectively causes apoptosis in c ... Full text Cite

HIV infection reveals widespread expansion of novel centromeric human endogenous retroviruses.

Journal Article Genome research · September 2013 Human endogenous retroviruses (HERVs) make up 8% of the human genome. The HERV-K (HML-2) family is the most recent group of these viruses to have inserted into the genome, and we have detected the activation of HERV-K (HML-2) proviruses in the blood of pat ... Full text Cite

Concise review: role of DEK in stem/progenitor cell biology.

Journal Article Stem cells (Dayton, Ohio) · August 2013 Understanding the factors that regulate hematopoiesis opens up the possibility of modifying these factors and their actions for clinical benefit. DEK, a non-histone nuclear phosphoprotein initially identified as a putative proto-oncogene, has recently been ... Full text Cite

Intercellular trafficking of the nuclear oncoprotein DEK.

Journal Article Proceedings of the National Academy of Sciences of the United States of America · April 2013 DEK is a biochemically distinct, conserved nonhistone protein that is vital to global heterochromatin integrity. In addition, DEK can be secreted and function as a chemotactic, proinflammatory factor. Here we show that exogenous DEK can penetrate cells, tr ... Full text Cite

Stacking the DEK: from chromatin topology to cancer stem cells.

Journal Article Cell cycle (Georgetown, Tex.) · January 2013 Stem cells are essential for development and tissue maintenance and display molecular markers and functions distinct from those of differentiated cell types in a given tissue. Malignant cells that exhibit stem cell-like activities have been detected in man ... Full text Cite

DEK expression in Merkel cell carcinoma and small cell carcinoma.

Journal Article Journal of cutaneous pathology · August 2012 BackgroundThe chromatin architectural factor DEK maps to chromosome 6p and is frequently overexpressed in several neoplasms, including small cell lung carcinoma, where it is associated with poor prognosis, tumor initiation activity and chemoresist ... Full text Cite

DEK regulates hematopoietic stem engraftment and progenitor cell proliferation.

Journal Article Stem cells and development · June 2012 DEK is a biochemically distinct protein that is generally found in the nucleus, where it is vital to global heterochromatin integrity. However, DEK is also secreted by cells (eg, macrophages) and influences other adjacent cells (eg, acts as a chemoattracta ... Full text Cite

DEK expression in melanocytic lesions.

Journal Article Human pathology · July 2011 The diagnosis of malignant melanoma presents a clinical challenge and relies principally on histopathological evaluation. Previous studies have indicated that increased expression of the DEK oncogene, a chromatin-bound factor, could contribute to the devel ... Full text Cite

The DEK oncoprotein is a Su(var) that is essential to heterochromatin integrity.

Journal Article Genes & development · April 2011 Heterochromatin integrity is crucial for genome stability and regulation of gene expression, but the factors involved in mammalian heterochromatin biology are only incompletely understood. Here we identify the oncoprotein DEK, an abundant nuclear protein w ... Full text Cite

DEK in the synovium of patients with juvenile idiopathic arthritis: characterization of DEK antibodies and posttranslational modification of the DEK autoantigen.

Journal Article Arthritis and rheumatism · February 2011 ObjectiveDEK is a nuclear phosphoprotein and autoantigen in a subset of children with juvenile idiopathic arthritis (JIA). Autoantibodies to DEK are also found in a broad spectrum of disorders associated with abnormal immune activation. We previou ... Full text Cite

High-affinity interaction of poly(ADP-ribose) and the human DEK oncoprotein depends upon chain length.

Journal Article Biochemistry · August 2010 Poly(ADP-ribose) polymerase-1 (PARP-1) is a molecular DNA damage sensor that catalyzes the synthesis of the complex biopolymer poly(ADP-ribose) (PAR) under consumption of NAD(+). PAR engages in fundamental cellular processes such as DNA metabolism and tran ... Full text Cite

Melanoma proliferation and chemoresistance controlled by the DEK oncogene.

Journal Article Cancer research · August 2009 Gain of chromosome 6p is a consistent feature of advanced melanomas. However, the identity of putative oncogene(s) associated with this amplification has remained elusive. The chromatin remodeling factor DEK is an attractive candidate as it maps to 6p (wit ... Full text Cite

DEK is a poly(ADP-ribose) acceptor in apoptosis and mediates resistance to genotoxic stress.

Journal Article Molecular and cellular biology · May 2008 DEK is a nuclear phosphoprotein implicated in oncogenesis and autoimmunity and a major component of metazoan chromatin. The intracellular cues that control the binding of DEK to DNA and its pleiotropic functions in DNA- and RNA-dependent processes have rem ... Full text Cite

Solution NMR structure of the N-terminal domain of the human DEK protein.

Journal Article Protein science : a publication of the Protein Society · February 2008 The human DEK protein has a long-standing association with carcinogenesis since the DEK gene was originally identified in the t(6:9) chromosomal translocation in a subtype of patients with acute myelogenous leukemia (AML). Recent studies have partly unveil ... Full text Cite

The DEK nuclear autoantigen is a secreted chemotactic factor.

Journal Article Molecular and cellular biology · December 2006 The nuclear DNA-binding protein DEK is an autoantigen that has been implicated in the regulation of transcription, chromatin architecture, and mRNA processing. We demonstrate here that DEK is actively secreted by macrophages and is also found in synovial f ... Full text Cite

Hypophosphorylation of the architectural chromatin protein DEK in death-receptor-induced apoptosis revealed by the isotope coded protein label proteomic platform.

Journal Article Proteomics · November 2006 During apoptosis nuclear morphology changes dramatically due to alterations of chromatin architecture and cleavage of structural nuclear proteins. To characterize early events in apoptotic nuclear dismantling we have performed a proteomic study of apoptoti ... Full text Cite

Gains and overexpression identify DEK and E2F3 as targets of chromosome 6p gains in retinoblastoma.

Journal Article Oncogene · September 2005 The paediatric eye tumour retinoblastoma is initiated by inactivation of RB1, a tumour suppressor on chromosome 13q. In addition to RB1 loss, many retinoblastomas show other genetic alterations including gains on chromosomes 6p21-pter and 1q31-q32. Recentl ... Full text Cite

The SAF-box domain of chromatin protein DEK.

Journal Article Nucleic acids research · January 2005 DEK is an abundant chromatin protein in metazoans reaching copy numbers of several millions/nucleus. Previous work has shown that human DEK, a protein of 375 amino acids, has two functional DNA-binding domains, of which one resides in a central part of the ... Full text Cite

The DEK protein--an abundant and ubiquitous constituent of mammalian chromatin.

Journal Article Gene · December 2004 The protein DEK is an abundant and ubiquitous chromatin protein in multicellular organisms (not in yeast). It is expressed in more than a million copies/nucleus of rapidly proliferating mammalian cells. DEK has two DNA binding modules of which one includes ... Full text Cite

Functional domains of the ubiquitous chromatin protein DEK.

Journal Article Molecular and cellular biology · July 2004 DEK was originally described as a proto-oncogene protein and is now known to be a major component of metazoan chromatin. DEK is able to modify the structure of DNA by introducing supercoils. In order to find interaction partners and functional domains of D ... Full text Cite

Phosphorylation by protein kinase CK2 changes the DNA binding properties of the human chromatin protein DEK.

Journal Article Molecular and cellular biology · July 2004 We have examined the posttranslational modification of the human chromatin protein DEK and found that DEK is phosphorylated by the protein kinase CK2 in vitro and in vivo. Phosphorylation sites were mapped by quadrupole ion trap mass spectrometry and found ... Full text Cite

Protein kinase CK2 phosphorylates the cell cycle regulatory protein Geminin.

Journal Article Biochemical and biophysical research communications · March 2004 Geminin contributes to cell cycle regulation by a timely inhibition of Cdt1p, the loading factor required for the assembly of pre-replication complexes. Geminin is expressed during S and G2 phase of the HeLa cell cycle and phosphorylated soon after its syn ... Full text Cite

Subcellular localization of the human proto-oncogene protein DEK.

Journal Article The Journal of biological chemistry · July 2001 Recent data revealed that DEK associates with splicing complexes through interactions mediated by serine/arginine-repeat proteins. However, the DEK protein has also been shown to change the topology of DNA in chromatin in vitro. This could indicate that th ... Full text Cite