Scholars@Duke
Francis Joseph Miller Jr.

Francis Joseph Miller Jr.

Adjunct Professor in the Department of Medicine
Medicine, Cardiology
203 Research Drive, 397 MSRB1, Durham, NC 27705
508 Fulton St, Bldg 15, Room 306, Durham, NC 27705

Selected Publications

Local intraluminal delivery of a smooth muscle-targeted RNA ligand inhibits neointima growth in a porcine model of peripheral vascular disease.

Journal Article Mol Ther Nucleic Acids · September 13, 2022 Anti-proliferative agents have been the primary therapeutic drug of choice to inhibit restenosis after endovascular treatment. However, recent safety and efficacy concerns for patients who underwent peripheral artery disease revascularization have demonstr ... Full text Link to item Cite

Drebrin attenuates atherosclerosis by limiting smooth muscle cell transdifferentiation.

Journal Article Cardiovasc Res · February 21, 2022 AIMS: The F-actin-binding protein Drebrin inhibits smooth muscle cell (SMC) migration, proliferation, and pro-inflammatory signalling. Therefore, we tested the hypothesis that Drebrin constrains atherosclerosis. METHODS AND RESULTS: SM22-Cre+/Dbnflox/flox/ ... Full text Open Access Link to item Cite

Outside-in Signaling by Adventitial Fibroblasts.

Journal Article Arterioscler Thromb Vasc Biol · February 2021 Full text Link to item Cite

Light sheet fluorescence microscopy as a new method for unbiased three-dimensional analysis of vascular injury.

Journal Article Cardiovasc Res · January 21, 2021 AIMS: Assessment of preclinical models of vascular disease is paramount in the successful translation of novel treatments. The results of these models have traditionally relied on two-dimensional (2D) histological methodologies. Light sheet fluorescence mi ... Full text Link to item Cite

Light Sheet Fluorescence Microscopy as a New Method for Unbiased Three-Dimensional Analysis of Vascular Injury

Journal Article · January 2, 2020 Abstract Aims Assessment of preclinical models of vascular disease are paramount in the successful translation of novel treatments. The results of these models have traditionally relied on 2-D histological methodologies. Light sheet fluorescence microscopy ... Full text Cite

Redox-regulation of NADPH Oxidase 4 in Cardiovascular Disease

Conference Free Radical Biology and Medicine · December 2019 Full text Cite

Delivery of Cell-Specific Aptamers to the Arterial Wall with an Occlusion Perfusion Catheter.

Journal Article Mol Ther Nucleic Acids · June 7, 2019 Current strategies to prevent restenosis following endovascular treatment include the local delivery of anti-proliferative agents to inhibit vascular smooth muscle cell (VSMC) proliferation and migration. These agents, not specific to VSMCs, are deposited ... Full text Link to item Cite

Nox2 NADPH oxidase is dispensable for platelet activation or arterial thrombosis in mice.

Journal Article Blood Adv · April 23, 2019 Deficiency of the Nox2 (gp91phox) catalytic subunit of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is a genetic cause of X-linked chronic granulomatous disease, a condition in which patients are prone to infection resulting from the loss of ... Full text Link to item Cite

Redox Activation of Nox1 (NADPH Oxidase 1) Involves an Intermolecular Disulfide Bond Between Protein Disulfide Isomerase and p47phox in Vascular Smooth Muscle Cells.

Journal Article Arterioscler Thromb Vasc Biol · February 2019 Objective- PDI (protein disulfide isomerase A1) was reported to support Nox1 (NADPH oxidase) activation mediated by growth factors in vascular smooth muscle cells. Our aim was to investigate the molecular mechanism by which PDI activates Nox1 and the funct ... Full text Link to item Cite

RNA inhibitors of nuclear proteins responsible for multiple organ dysfunction syndrome.

Journal Article Nat Commun · January 10, 2019 Featured Publication The development of multiple organ dysfunction syndrome (MODS) following infection or tissue injury is associated with increased patient morbidity and mortality. Extensive cellular injury results in the release of nuclear proteins, of which histones are the ... Full text Link to item Cite

Drebrin regulates angiotensin II-induced aortic remodelling.

Journal Article Cardiovasc Res · November 1, 2018 AIMS: The actin-binding protein Drebrin is up-regulated in response to arterial injury and reduces smooth muscle cell (SMC) migration and proliferation through its interaction with the actin cytoskeleton. We, therefore, tested the hypothesis that SMC Drebr ... Full text Open Access Link to item Cite

The Regulation Of Nox In Vascular Disease

Conference Free Radical Biology and Medicine · November 2017 Full text Cite

Long Noncoding RNA MANTIS Facilitates Endothelial Angiogenic Function.

Journal Article Circulation · July 4, 2017 BACKGROUND: The angiogenic function of endothelial cells is regulated by numerous mechanisms, but the impact of long noncoding RNAs (lncRNAs) has hardly been studied. We set out to identify novel and functionally important endothelial lncRNAs. METHODS: Epi ... Full text Link to item Cite

Smooth Muscle Cell-targeted RNA Aptamer Inhibits Neointimal Formation.

Journal Article Mol Ther · April 2016 Inhibition of vascular smooth muscle cell (VSMC) proliferation by drug eluting stents has markedly reduced intimal hyperplasia and subsequent in-stent restenosis. However, the effects of antiproliferative drugs on endothelial cells (EC) contribute to delay ... Full text Link to item Cite

Nox1 in cardiovascular diseases: regulation and pathophysiology.

Journal Article Clin Sci (Lond) · February 2016 Since its discovery in 1999, a number of studies have evaluated the role of Nox1 NADPH oxidase in the cardiovascular system. Nox1 is activated in vascular cells in response to several different agonists, with its activity regulated at the transcriptional l ... Full text Link to item Cite

Chemiluminescence and the Nox1-Nox2-Nox4 Triple Knockout.

Journal Article Antioxid Redox Signal · November 20, 2015 Full text Link to item Cite

Cell-internalization SELEX: method for identifying cell-internalizing RNA aptamers for delivering siRNAs to target cells.

Journal Article Methods Mol Biol · 2015 After a decade of work to address cellular uptake, the principal obstacle to RNAi-based therapeutics, there is now well-deserved, renewed optimism about RNAi-based drugs. Phase I and II studies have shown safe, strong, and durable-gene knockdown (80-90%, l ... Full text Link to item Cite

Phosphorylation of Nox1 regulates association with NoxA1 activation domain.

Journal Article Circ Res · November 7, 2014 RATIONALE: Activation of Nox1 initiates redox-dependent signaling events crucial in the pathogenesis of vascular disease. Selective targeting of Nox1 is an attractive potential therapy, but requires a better understanding of the molecular modifications con ... Full text Link to item Cite

Role of NADPH oxidase and xanthine oxidase in mediating inducible VT/VF and triggered activity in a canine model of myocardial ischemia.

Journal Article Int J Mol Sci · November 4, 2014 BACKGROUND: Ventricular tachycardia or fibrillation (VT/VF) of focal origin due to triggered activity (TA) from delayed afterdepolarizations (DADs) is reproducibly inducible after anterior coronary artery occlusion. Both VT/VF and TA can be blocked by redu ... Full text Link to item Cite

Canonical Wnt signaling induces vascular endothelial dysfunction via p66Shc-regulated reactive oxygen species.

Journal Article Arterioscler Thromb Vasc Biol · October 2014 OBJECTIVE: Reactive oxygen species regulate canonical Wnt signaling. However, the role of the redox regulatory protein p66(Shc) in the canonical Wnt pathway is not known. We investigated whether p66(Shc) is essential for canonical Wnt signaling in the endo ... Full text Link to item Cite

Endothelial dysfunction in chronic inflammatory diseases.

Journal Article Int J Mol Sci · June 25, 2014 Chronic inflammatory diseases are associated with accelerated atherosclerosis and increased risk of cardiovascular diseases (CVD). As the pathogenesis of atherosclerosis is increasingly recognized as an inflammatory process, similarities between atheroscle ... Full text Link to item Cite

Oxidative stress in cardiovascular disease.

Journal Article Int J Mol Sci · April 9, 2014 In the special issue "Oxidative Stress in Cardiovascular Disease" authors were invited to submit papers that investigate key questions in the field of cardiovascular free radical biology. The original research articles included in this issue provide import ... Full text Link to item Cite

Nox1 NADPH oxidase is necessary for late but not early myocardial ischaemic preconditioning.

Journal Article Cardiovasc Res · April 1, 2014 AIMS: Ischaemic preconditioning (IPC) is an adaptive mechanism that renders the myocardium resistant to injury from subsequent hypoxia. Although reactive oxygen species (ROS) contribute to both the early and late phases of IPC, their enzymatic source and a ... Full text Link to item Cite

Nox4 NADPH oxidase contributes to smooth muscle cell phenotypes associated with unstable atherosclerotic plaques.

Journal Article Redox Biol · 2014 Plaque instability associated with acute coronary syndromes results in part from apoptosis and senescence of cells within the atherosclerotic (AS) lesion. Increased cellular oxidative stress has been proposed to contribute to plaque progression and changes ... Full text Link to item Cite

NOX4 mediates cytoprotective autophagy induced by the EGFR inhibitor erlotinib in head and neck cancer cells.

Journal Article Toxicol Appl Pharmacol · November 1, 2013 Most head and neck squamous cell carcinomas (HNSCCs) overexpress epidermal growth factor receptor (EGFR) and EGFR inhibitors are routinely used in the treatment of HNSCC. However, many HNSCC tumors do not respond or become refractory to EGFR inhibitors. Au ... Full text Link to item Cite

Nox1 Endocytosis and Activation Are Regulated by Intracellular Hydrophobic Motifs

Journal Article Free Radical Biology and Medicine · November 2013 Full text Cite

The epidermal growth factor receptor and its ligands in cardiovascular disease.

Journal Article Int J Mol Sci · October 15, 2013 The epidermal growth factor receptor (EGFR) family and its ligands serve as a switchboard for the regulation of multiple cellular processes. While it is clear that EGFR activity is essential for normal cardiac development, its function in the vasculature a ... Full text Link to item Cite

Inhibition of NADPH oxidase by apocynin attenuates progression of atherosclerosis.

Journal Article Int J Mol Sci · August 19, 2013 Of the multiple sources of reactive oxygen species (ROS) in the blood vessel, NADPH oxidases are the primary source. Whereas several studies have implicated NADPH oxidases in the initiation of atherosclerosis, their roles in disease progression are incompl ... Full text Link to item Cite

CaMKII is essential for the proasthmatic effects of oxidation.

Journal Article Sci Transl Med · July 24, 2013 Increased reactive oxygen species (ROS) contribute to asthma, but little is known about the molecular mechanisms connecting increased ROS with characteristic features of asthma. We show that enhanced oxidative activation of the Ca(2+)/calmodulin-dependent ... Full text Link to item Cite

Opportunity nox: the future of NADPH oxidases as therapeutic targets in cardiovascular disease.

Journal Article Cardiovasc Ther · June 2013 Over 40 years ago, NADPH (nicotinamide adenine dinucleotide phosphate) oxidase 2 (Nox2) was discovered in phagocytes and found to be essential in innate immunity. More than 20 years passed before additional Nox isoforms were discovered; and since then, stu ... Full text Link to item Cite

Hydrogen peroxide promotes aging-related platelet hyperactivation and thrombosis.

Journal Article Circulation · March 26, 2013 BACKGROUND: The incidence of thrombotic events increases during aging, but the mechanisms are not well understood. To investigate the prothrombotic role of oxidative stress during aging, we tested the hypothesis that aged mice overexpressing the antioxidan ... Full text Link to item Cite

Extracellular but not cytosolic superoxide dismutase protects against oxidant-mediated endothelial dysfunction.

Journal Article Redox Biol · 2013 Superoxide (O2 (•-)) contributes to the development of cardiovascular disease. Generation of O2 (•-) occurs in both the intracellular and extracellular compartments. We hypothesized that the gene transfer of cytosolic superoxide dismutase (SOD1) or extrace ... Full text Link to item Cite

The multifunctional Ca²⁺/calmodulin-dependent kinase IIδ (CaMKIIδ) regulates arteriogenesis in a mouse model of flow-mediated remodeling.

Journal Article PLoS One · 2013 OBJECTIVE: Sustained hemodynamic stress mediated by high blood flow promotes arteriogenesis, the outward remodeling of existing arteries. Here, we examined whether Ca²⁺/calmodulin-dependent kinase II (CaMKII) regulates arteriogenesis. METHODS AND RESULTS: ... Full text Link to item Cite

NOX2 protects against prolonged inflammation, lung injury, and mortality following systemic insults.

Journal Article J Innate Immun · 2013 The systemic inflammatory response syndrome (SIRS) is a clinical condition occurring in intensive care unit patients as a consequence of both infectious and noninfectious insults. The mechanisms underlying resolution of SIRS are not well characterized. NOX ... Full text Link to item Cite

Nox1 Phosphorylation in Cardiovascular Disease

Conference Free Radical Biology and Medicine · November 2012 Full text Cite

Increased epidermal growth factor-like ligands are associated with elevated vascular nicotinamide adenine dinucleotide phosphate oxidase in a primate model of atherosclerosis.

Journal Article Arterioscler Thromb Vasc Biol · October 2012 OBJECTIVE: To characterize the relationship between the expression of epidermal growth factor (EGF)-like ligands and vascular nicotinamide adenine dinucleotide phosphate (NADPH) oxidase expression and activity in a primate model of atherosclerosis. METHODS ... Full text Link to item Cite

β-Adrenergic receptor antagonists ameliorate myocyte T-tubule remodeling following myocardial infarction.

Journal Article FASEB J · June 2012 β-Adrenergic receptor (AR) blockers provide substantial clinical benefits, including improving overall survival and left ventricular (LV) function following myocardial infarction (MI), though the mechanisms remain incompletely defined. The transverse-tubul ... Full text Link to item Cite

Nox1 transactivation of epidermal growth factor receptor promotes N-cadherin shedding and smooth muscle cell migration.

Journal Article Cardiovasc Res · March 1, 2012 AIMS: In atherosclerosis and restenosis, vascular smooth muscle cells (SMCs) migrate into the subendothelial space and proliferate, contributing to neointimal formation. The goal of this study was to define the signalling pathway by which Nox1 NAPDH oxidas ... Full text Link to item Cite

Rapid identification of cell-specific, internalizing RNA aptamers with bioinformatics analyses of a cell-based aptamer selection.

Journal Article PLoS One · 2012 BACKGROUND: The broad applicability of RNA aptamers as cell-specific delivery tools for therapeutic reagents depends on the ability to identify aptamer sequences that selectively access the cytoplasm of distinct cell types. Towards this end, we have develo ... Full text Link to item Cite

Increased expression of Nox1 in neointimal smooth muscle cells promotes activation of matrix metalloproteinase-9.

Journal Article J Vasc Res · 2012 OBJECTIVE: Vascular injury causes neointimal hypertrophy, which is characterized by redox-mediated matrix degradation and smooth muscle cell (SMC) migration and proliferation. We hypothesized that, as compared to the adjacent medial SMCs, neointimal SMCs p ... Full text Link to item Cite

Reactive oxygen species: from health to disease.

Journal Article Swiss Med Wkly · 2012 Upon reaction with electrons, oxygen is transformed into reactive oxygen species (ROS). It has long been known that ROS can destroy bacteria and destroy human cells, but research in recent decades has highlighted new roles for ROS in health and disease. In ... Full text Link to item Cite

Statin therapy reduces growth of abdominal aortic aneurysms.

Journal Article J Investig Med · December 2011 BACKGROUND: The aim of this study was to evaluate the influence of statins on the growth of small abdominal aortic aneurysms (AAAs). METHODS: We retrospectively examined AAA diameter in 211 patients who had undergone serial imaging surveillance. RESULTS: P ... Full text Link to item Cite

Activation of NADPH oxidase 1 increases intracellular calcium and migration of smooth muscle cells.

Journal Article Hypertension · September 2011 Redox-dependent migration and proliferation of vascular smooth muscle cells (SMCs) are central events in the development of vascular proliferative diseases; however, the underlying intracellular signaling mechanisms are not fully understood. We tested the ... Full text Link to item Cite

Role for Nox1 NADPH oxidase in atherosclerosis.

Journal Article Atherosclerosis · June 2011 OBJECTIVE: Examine the contribution of Nox1 NADPH oxidase to atherogenesis. METHODS AND RESULTS: Male apolipoprotein E deficient mice (ApoE(-/-)) and male mice deficient in both apolipoprotein E and Nox1 (ApoE(-/-) Nox1(-/y)) received an atherogenic diet f ... Full text Link to item Cite

Erlotinib-mediated inhibition of EGFR signaling induces metabolic oxidative stress through NOX4.

Journal Article Cancer Res · June 1, 2011 Redox regulation of epidermal growth factor receptor (EGFR) signaling helps protect cells against oxidative stress. In this study, we investigated whether the cytotoxicity of an EGFR tyrosine kinase inhibitor, erlotinib (ERL), was mediated by induction of ... Full text Link to item Cite

Any questions? A concise guide to navigating the Q&A session after a presentation.

Journal Article EMBO Rep · March 2011 Talks and presentations are an integral part of a scientist's life; conducting an engaging and mutually beneficial Q&A session after a talk is as important as the talk itself. ... Full text Link to item Cite

A critical role for chloride channel-3 (CIC-3) in smooth muscle cell activation and neointima formation.

Journal Article Arterioscler Thromb Vasc Biol · February 2011 OBJECTIVE: We have shown that the chloride-proton antiporter chloride channel-3 (ClC-3) is required for endosome-dependent signaling by the Nox1 NADPH oxidase in SMCs. In this study, we tested the hypothesis that ClC-3 is necessary for proliferation of smo ... Full text Link to item Cite

Glutathione peroxidase-deficient smooth muscle cells cause paracrine activation of normal smooth muscle cells via cyclophilin A.

Journal Article Vascul Pharmacol · 2011 BACKGROUND/AIMS: Reduced activity of the antioxidant glutathione peroxidase-1 (GPx1) correlates with increased risk of cardiovascular events in patients with coronary artery disease. However, it remains unclear whether this imbalance in antioxidant capacit ... Full text Link to item Cite

An oxidized extracellular oxidation-reduction state increases Nox1 expression and proliferation in vascular smooth muscle cells via epidermal growth factor receptor activation.

Journal Article Arterioscler Thromb Vasc Biol · November 2010 OBJECTIVE: To examine the effect of an oxidized extracellular oxidation-reduction (redox) state (E(h)) on the expression of NADPH oxidases in vascular cells. METHODS AND RESULTS: The generation of reactive oxygen species by NADPH oxidase (Nox)-based NADPH ... Full text Link to item Cite

Activation of swelling-activated chloride current by tumor necrosis factor-alpha requires ClC-3-dependent endosomal reactive oxygen production.

Journal Article J Biol Chem · July 23, 2010 ClC-3 is a Cl(-)/H(+) antiporter required for cytokine-induced intraendosomal reactive oxygen species (ROS) generation by Nox1. ClC-3 current is distinct from the swelling-activated chloride current (ICl(swell)), but overexpression of ClC-3 can activate cu ... Full text Link to item Cite

A differential role for endocytosis in receptor-mediated activation of Nox1.

Journal Article Antioxid Redox Signal · March 1, 2010 Internalization of activated receptors to a compartment enriched with NAPDH oxidase and associated signaling molecules is expected to facilitate regulation of redox-mediated signal transduction. The aim of this study was to test the hypothesis that endocyt ... Full text Link to item Cite

Nox4 NADPH Oxidase Modulates the Intracellular Redox State

Conference Free Radical Biology and Medicine · January 2010 Full text Cite

Electrophysiology of reactive oxygen production in signaling endosomes.

Journal Article Antioxid Redox Signal · June 2009 Endosome trafficking and function require acidification by the vacuolar ATPase (V-ATPase). Electrogenic proton (H+) transport reduces the pH and creates a net positive charge in the endosomal lumen. Concomitant chloride (Cl-) influx has been proposed to oc ... Full text Link to item Cite

Free radical scavenger specifically prevents ischemic focal ventricular tachycardia.

Journal Article Heart Rhythm · April 2009 BACKGROUND: Focal ventricular tachycardia (VT) in acute myocardial ischemia is closely related to triggered activity (TA), which may be blocked by scavenging reactive oxygen species (ROS). OBJECTIVE: This study analyzed effects of acutely administered ROS ... Full text Link to item Cite

Inhibition of apoptotic signaling and neointimal hyperplasia by tempol and nitric oxide synthase following vascular injury.

Journal Article J Vasc Res · 2009 OBJECTIVES: We hypothesized that redox-mediated apoptosis of medial smooth muscle cells (SMC) during the acute phase of vascular injury contributes to the pathophysiology of vascular disease. METHODS: Apoptosis of medial SMC (1-14 days following balloon in ... Full text Link to item Cite

Prophylactic Beta Blockade To Prevent Peri-Extubation Cardiac Ischemia and Congestive Heart Failure

Journal Article AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · January 1, 2009 Link to item Cite

Intracellular protein aggregation is a proximal trigger of cardiomyocyte autophagy.

Journal Article Circulation · June 17, 2008 BACKGROUND: Recent reports demonstrate that multiple forms of cardiovascular stress, including pressure overload, chronic ischemia, and infarction-reperfusion injury, provoke an increase in autophagic activity in cardiomyocytes. However, nothing is known r ... Full text Link to item Cite

Coronary constriction to angiotensin II is enhanced by endothelial superoxide production in sheep programmed by dexamethasone.

Journal Article Pediatr Res · April 2008 Early gestation dexamethasone (dex) administration is an ovine model of fetal programming associated with increased coronary reactivity to angiotensin II (Ang II). NADPH oxidase-dependent superoxide production plays an important role in both Ang II signali ... Full text Link to item Cite

Endothelial superoxide production is altered in sheep programmed by early gestation dexamethasone exposure.

Journal Article Neonatology · 2008 BACKGROUND: Animal models have demonstrated that maternal undernutrition or early gestation glucocorticoid exposure induces endothelial dysfunction in the offspring. OBJECTIVES: We sought to determine whether early gestation dexamethasone (DEX) exposure is ... Full text Link to item Cite

PATHOLOGICAL ROLE OF Nox PROTEINS

Conference FREE RADICAL BIOLOGY AND MEDICINE · 2008 Cite

Role for Nox1 NADPH oxidase in atherosclerosis

Journal Article CIRCULATION · October 16, 2007 Link to item Cite

Cytokine activation of nuclear factor kappa B in vascular smooth muscle cells requires signaling endosomes containing Nox1 and ClC-3.

Journal Article Circ Res · September 28, 2007 Reactive oxygen species (ROS) are mediators of intracellular signals for a myriad of normal and pathologic cellular events, including differentiation, hypertrophy, proliferation, and apoptosis. NADPH oxidases are important sources of ROS that are present i ... Full text Link to item Cite

Increased plasma oxidized phospholipid:apolipoprotein B-100 ratio with concomitant depletion of oxidized phospholipids from atherosclerotic lesions after dietary lipid-lowering: a potential biomarker of early atherosclerosis regression.

Journal Article Arterioscler Thromb Vasc Biol · January 2007 BACKGROUND: Oxidized phospholipids (OxPL) are pro-inflammatory. We evaluated whether changes in plasma levels of OxPL associated with apolipoprotein B-100 (apoB-100) reflect changes in OxPL content in atherosclerotic plaques during dietary-induced atherosc ... Full text Link to item Cite

Deletion of p47phox attenuates angiotensin II-induced abdominal aortic aneurysm formation in apolipoprotein E-deficient mice.

Journal Article Circulation · August 1, 2006 BACKGROUND: Angiotensin II (Ang II) contributes to vascular pathology in part by stimulating NADPH oxidase activity, leading to increased formation of superoxide (O2-). We reported that O2- levels, NADPH oxidase activity, and expression of the p47phox subu ... Full text Link to item Cite

Tempol therapy attenuates medial smooth muscle cell apoptosis and neointima formation after balloon catheter injury in carotid artery of diabetic rats.

Journal Article Am J Physiol Heart Circ Physiol · September 2005 Accumulating data support the hypothesis that reactive oxygen species (ROS) play a critical role in the vascular complications observed in diabetes. However, the mechanisms of ROS-mediated vascular complications in diabetes are not clear. We tested the hyp ... Full text Link to item Cite

Vitamin E inhibits abdominal aortic aneurysm formation in angiotensin II-infused apolipoprotein E-deficient mice.

Journal Article Arterioscler Thromb Vasc Biol · August 2005 BACKGROUND: Abdominal aortic aneurysms (AAAs) in humans are associated with locally increased oxidative stress and activity of NADPH oxidase. We investigated the hypothesis that vitamin E, an antioxidant with documented efficacy in mice, can attenuate AAA ... Full text Link to item Cite

Early gestation dexamethasone programs enhanced postnatal ovine coronary artery vascular reactivity.

Journal Article Am J Physiol Regul Integr Comp Physiol · January 2005 Excessive exposure of the fetus to maternally derived corticosteroids has been linked to the development of adult-onset diseases. To determine if early gestation corticosteroid exposure alters subsequent coronary artery reactivity, we administered dexameth ... Full text Link to item Cite

Regulation of endotoxin-induced proinflammatory activation in human coronary artery cells: expression of functional membrane-bound CD14 by human coronary artery smooth muscle cells.

Journal Article J Immunol · July 15, 2004 Low-level endotoxemia has been identified as a powerful risk factor for atherosclerosis. However, little is known about the mechanisms that regulate endotoxin responsiveness in vascular cells. We conducted experiments to compare the relative responses of h ... Full text Link to item Cite

The nitric oxide synthase inhibitor N(G)-nitro-L-arginine decreases defibrillation-induced free radical generation.

Journal Article Resuscitation · March 2004 OBJECTIVES: to demonstrate that nitric oxide (NO) contributes to free radical generation after epicardial shocks and to determinethe effect of a nitric oxide synthase (NOS) inhibitor, N(G)-nitro-L-arginine (L-NNA), on free radical generation. BACKGROUND: F ... Full text Link to item Cite

ROLE OF THE GP91PHOX HOMOLOGUES NOX1 AND NOX4 IN ATHEROSCLEROSIS.

Conference Journal of Investigative Medicine · March 2004 Full text Cite

THE EFFECT OF SUPEROXIDE AND HYDROGEN PEROXIDE IN ATHEROSCLEROSIS.

Conference Journal of Investigative Medicine · March 2004 Full text Cite

Reactive oxygen species mediate arachidonic acid-induced dilation in porcine coronary microvessels.

Journal Article Am J Physiol Heart Circ Physiol · December 2003 Reactive oxygen species (ROS) have been proposed to mediate vasodilation in the microcirculation. We investigated the role of ROS in arachidonic acid (AA)-induced coronary microvascular dilation. Porcine epicardial coronary arterioles (110 +/- 4 microm dia ... Full text Link to item Cite

The nitric oxide donor S-nitroso-N-acetylpenicillamine (SNAP) increases free radical generation and degrades left ventricular function after myocardial ischemia-reperfusion.

Journal Article Resuscitation · December 2003 BACKGROUND: During reperfusion of ischemic myocardium nitric oxide (NO) reacts with superoxide radicals to form cardiotoxic peroxynitrite, which causes lipid peroxidation. Our hypothesis was that infusion of a NO donor S-nitroso-N-acetylpenicillamine (SNAP ... Full text Link to item Cite

Gp91phox contributes to NADPH oxidase activity in aortic fibroblasts but not smooth muscle cells.

Journal Article Am J Physiol Heart Circ Physiol · December 2003 Reactive oxygen species (ROS) derived from vascular NADPH oxidase are important in normal and pathological regulation of vessel growth and function. Cell-specific differences in expression and function of the catalytic subunit of NADPH oxidase may contribu ... Full text Link to item Cite

Low-level endotoxin induces potent inflammatory activation of human blood vessels: inhibition by statins.

Journal Article Arterioscler Thromb Vasc Biol · September 1, 2003 BACKGROUND: Low-level endotoxemia (ie, >or=50 pg/mL) in apparently healthy subjects was recently identified as a powerful, independent risk factor for atherosclerosis. METHODS AND RESULTS: We treated human saphenous veins (HSVs) with low levels of endotoxi ... Full text Link to item Cite

The nitric oxide synthase inhibitor N(G)-nitro-L-arginine decreases defibrillation-induced free radical generation.

Journal Article Resuscitation · April 2003 OBJECTIVES: To demonstrate that nitric oxide (NO) contributes to free radical generation after epicardial shocks and to determine the effect of a nitric oxide synthase (NOS) inhibitor, N(G)-nitro-L-arginine (L-NNA), on free radical generation. BACKGROUND: ... Full text Link to item Cite

Activation of NAD(P)H oxidase by lipid hydroperoxides: mechanism of oxidant-mediated smooth muscle cytotoxicity.

Journal Article Free Radic Biol Med · April 1, 2003 Oxidized lipids, such as 13-hydroperoxyoctadecadienoic acid (13-HPODE), have been implicated in the pathogenesis of atherosclerosis. 13-HPODE, a constituent of oxidized low-density lipoproteins, can induce cytotoxicity of vascular smooth muscle cells (SMC) ... Full text Link to item Cite

Apocynin inhibits atherosclerosis in mice

Conference FASEB JOURNAL · March 14, 2003 Link to item Cite

The role of antioxidant-1 in extracellular superoxide dismutase function

Conference FREE RADICAL BIOLOGY AND MEDICINE · January 1, 2003 Link to item Cite

Aortic aneurysms: It's all about the stress.

Journal Article Arterioscler Thromb Vasc Biol · December 1, 2002 Full text Link to item Cite

Anticoagulant responses to thrombin are enhanced during regression of atherosclerosis in monkeys.

Journal Article Circulation · August 13, 2002 BACKGROUND: Diet-induced atherosclerosis in monkeys produces abnormal anticoagulant responses to thrombin, including decreased generation of activated protein C (APC). We tested the hypothesis that anticoagulant responses to thrombin increase toward normal ... Full text Link to item Cite

The vascular NADPH oxidase subunit p47phox is involved in redox-mediated gene expression.

Journal Article Free Radic Biol Med · June 1, 2002 An NADPH oxidase is thought to be a main source of vascular superoxide (O(2)(-)) production. The functional role of this oxidase, however, and the contribution of the different subunits of the enzyme to cellular signaling are still incompletely understood. ... Full text Link to item Cite

Oxidative stress in human abdominal aortic aneurysms: a potential mediator of aneurysmal remodeling.

Journal Article Arterioscler Thromb Vasc Biol · April 1, 2002 Abdominal aortic aneurysm (AAA) is an inflammatory disorder characterized by localized connective tissue degradation and smooth muscle cell (SMC) apoptosis, leading to aortic dilatation and rupture. Reactive oxygen species are abundantly produced during in ... Full text Link to item Cite

Regression of atherosclerosis in monkeys reduces vascular superoxide levels.

Journal Article Circ Res · February 22, 2002 Superoxide (O2*-) in arteries may contribute to atherosclerosis in part by inactivation of nitric oxide. We hypothesized that regression of atherosclerosis in nonhuman primates is associated with a decrease in vascular NAD(P)H oxidase, decreased O2*- level ... Full text Link to item Cite

H(2)O(2)-induced O(2) production by a non-phagocytic NAD(P)H oxidase causes oxidant injury.

Journal Article J Biol Chem · August 3, 2001 Non-phagocytic NAD(P)H oxidases have been implicated as major sources of reactive oxygen species in blood vessels. These oxidases can be activated by cytokines, thereby generating O(2), which is subsequently converted to H(2)O(2) and other oxidant species. ... Full text Link to item Cite

Nitric oxide synthase inhibitors decrease coronary sinus-free radical concentration and ameliorate myocardial stunning in an ischemia-reperfusion model.

Journal Article J Am Coll Cardiol · August 2001 OBJECTIVES: Our objective was to determine the effect of a nitric oxide synthase inhibitor, NG-nitro-L-arginine (L-NNA) on free radical generation and myocardial contractility after ischemia-reperfusion. BACKGROUND: Cardiotoxic free radicals are generated ... Full text Link to item Cite

Calcium-activated potassium channels mask vascular dysfunction associated with oxidized LDL exposure in rabbit aorta.

Journal Article Jpn Heart J · May 2001 Endothelium-dependent vasodilation is impaired in atherosclerosis. Oxidized low density lipoprotein (ox-LDL) plays an important role, possibly through alterations in G-protein activation. We examined the effect of acute exposure to ox-LDL on the dilator re ... Full text Link to item Cite

Adventitial fibroblasts: backstage journeymen.

Journal Article Arterioscler Thromb Vasc Biol · May 2001 Full text Link to item Cite

Percutaneous sclerotherapy for congenital venous malformations in the extremities.

Journal Article Orthopedics · January 2001 Between January 1991 and January 1998, a total of 15 patients underwent direct injection sclerotherapy for painful peripheral venous malformations. Duplex ultrasonography or venography was used in all cases for the detection and localization of tortuous ve ... Full text Link to item Cite

Quinacrine inhibits development of atherosclerosis in mice.

Journal Article JOURNAL OF INVESTIGATIVE MEDICINE · September 1, 2000 Link to item Cite

Clinical manifestations in a large hereditary hemorrhagic telangiectasia (HHT) type 2 kindred.

Journal Article Am J Med Genet · August 14, 2000 HHT type 2 (HHT 2) is a multi-system vascular dysplasia caused by a mutation in the ALK-1 gene, but the phenotype has not been well defined. We report on 51 members of an HHT 2 kindred with an ALK-1 gene mutation shown to be associated with the disorder. T ... Full text Link to item Cite

Enhanced H(2)O(2)-induced cytotoxicity in "epithelioid" smooth muscle cells: implications for neointimal regression.

Journal Article Arterioscler Thromb Vasc Biol · June 2000 Vascular smooth muscle cells (SMCs) are phenotypically diverse. Although most medial SMCs can be classified as "fusiform," others are of the "epithelioid" phenotype. Proliferation and apoptosis of epithelioid SMCs may contribute importantly to neointimal f ... Full text Link to item Cite

Gene transfer of endothelial nitric oxide synthase improves relaxation of carotid arteries from diabetic rabbits.

Journal Article Circulation · March 7, 2000 BACKGROUND: Diabetes mellitus is associated with impairment of NO-mediated vascular relaxation. The purpose of this study was to determine whether adenovirus-mediated gene transfer of endothelial NO synthase (eNOS) or Cu/Zn superoxide dismutase (SOD1) impr ... Full text Link to item Cite

Gene transfer of endothelial nitric oxide synthase reduces angiotensin II-induced endothelial dysfunction.

Journal Article Hypertension · February 2000 Angiotensin II stimulates vascular NADPH oxidase to produce superoxide, which can react with nitric oxide and impair vasomotor function. We tested the hypothesis that the overexpression of endothelial nitric oxide synthase (eNOS) or superoxide dismutase (S ... Full text Link to item Cite

Overexpression of human catalase inhibits proliferation and promotes apoptosis in vascular smooth muscle cells.

Journal Article Circ Res · September 17, 1999 The role of reactive oxygen species, such as superoxide anions (O(2). (-)) and hydrogen peroxide (H(2)O(2)), in modulating vascular smooth muscle cell proliferation and viability is controversial. To investigate the role of endogenously produced H(2)O(2), ... Full text Link to item Cite

Nucleo-cytoplasmic interactions that control nuclear envelope breakdown and entry into mitosis in the sea urchin zygote.

Journal Article J Cell Sci · April 1999 In sea urchin zygotes and mammalian cells nuclear envelope breakdown (NEB) is not driven simply by a rise in cytoplasmic cyclin dependent kinase 1-cyclin B (Cdk1-B) activity; the checkpoint monitoring DNA synthesis can prevent NEB in the face of mitotic le ... Full text Link to item Cite

Smooth muscle cell production of superoxide is increased in atherosclerosis.

Journal Article JOURNAL OF INVESTIGATIVE MEDICINE · September 1, 1998 Link to item Cite

Superoxide production in vascular smooth muscle contributes to oxidative stress and impaired relaxation in atherosclerosis.

Journal Article Circ Res · June 29, 1998 The endothelium is a source of reactive oxygen species in short-term models of hypercholesterolemia and atherosclerosis. We examined a chronic model of atherosclerosis for increased vascular production of superoxide (O2-.) and determined whether endothelia ... Full text Link to item Cite

Pharmacologic activation of the human coronary microcirculation in vitro: endothelium-dependent dilation and differential responses to acetylcholine.

Journal Article Cardiovasc Res · June 1998 OBJECTIVES: In vivo studies of the human coronary resistance circulation cannot control for indirect effects of myocardial metabolism, compression, and neurohumoral influences. This study directly examined the vasodilator responses of the human coronary mi ... Full text Link to item Cite

Gene transfer of superoxide dismutase protects against free radical mediated impairment of vasodilation

Journal Article FASEB Journal · March 20, 1998 Reactive oxygen species play an important role in mediating vascular dysfunction in disease. Gene transfer of antioxidants to blood vessels has the potential to improve vascular function. We hypothesized that gene transfer of superoxide dismutase (SOD) to ... Cite

Myogenic constriction of human coronary arterioles.

Journal Article Am J Physiol · July 1997 Myogenic constriction is an important mechanism of blood flow regulation; however, it has never been demonstrated in the human coronary circulation. We examined responses of human coronary resistance vessels in vitro to changes in intraluminal pressure and ... Full text Link to item Cite

Myogenic constriction of human coronary arterioles

Journal Article AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY · July 1, 1997 Link to item Cite

DIFFERENTIAL EFFECT OF ACETYLCHOLINE ON ATRIAL AND VENTRICULAR RESISTANCE VESSELS ISOLATED FROM HUMAN HEARTS

Journal Article JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY · February 1, 1994 Link to item Cite

ISOLATED HUMAN CORONARY ARTERIOLES EXHIBIT MYOGENIC TONE

Journal Article CIRCULATION · October 1, 1993 Link to item Cite

Effect of brief myocardial ischemia on sympathetic coronary vasoconstriction.

Journal Article Circ Res · October 1992 The purpose of the present study was to determine whether sympathetic coronary vasoconstrictor responses are altered after brief ischemia and reperfusion. Adult mongrel dogs were anesthetized and instrumented for measurements of heart rate, arterial pressu ... Full text Link to item Cite

Activation in the region of parabrachial nucleus elicits neurogenically mediated coronary vasoconstriction.

Journal Article Am J Physiol · November 1991 A role for parabrachial nucleus in cardiovascular regulation is suggested by evidence that electrical stimulation in this region elicits increase in heart rate and arterial pressure. We hypothesized that parabrachial nucleus may also be involved in control ... Full text Link to item Cite

Activation in the region of parabrachial nucleus elicits neurogenically mediated coronary vasoconstriction

Journal Article American Journal of Physiology - Heart and Circulatory Physiology · January 1, 1991 A role for parabrachial nucleus in cardiovascular regulation is suggested by evidence that electrical stimulation in this region elicits increases in heart rate and arterial pressure. We hypothesized that parabrachial nucleus may also be involved in contro ... Full text Cite

CEREBELLAR REGULATION OF CORONARY BLOOD-FLOW

Journal Article CLINICAL RESEARCH · October 1, 1987 Link to item Cite

Uncontrollable postpartum bleeding: a new approach to hemostasis through angiographic arterial embolization.

Journal Article Obstet Gynecol · September 1979 A case of severe postpartum hemorrhage is reported. Three separate surgical procedures failed to reveal the source of bleeding, and standard surgical techniques, including bilateral ligation of the hypogastric arteries, were unsuccessful in producing hemos ... Link to item Cite