Scholars@Duke
John Paul Middleton

John Paul Middleton

Professor of Medicine
Medicine, Nephrology
j.p.middleton@duke.edu
Duke Box 2747, Durham, NC 27705
2424 Erwin Road, Suite 605, Durham, NC 27705

Selected Publications

Randomized Trial of Patiromer on Efficacy to Reduce Episodic Hyperkalemia in Patients with ESKD Treated With Hemodialysis

Journal Article Kidney International Reports · November 1, 2024 Introduction: Individuals with end-stage kidney disease (ESKD) maintained on hemodialysis (HD) carry a high risk of cardiac arrhythmias. This risk is heightened by episodic hyperkalemia. The purpose of the study was to investigate whether patiromer adminis ... Full text Cite

Associations of Kidney Functional Magnetic Resonance Imaging Biomarkers with Markers of Inflammation in Individuals with CKD.

Journal Article Kidney360 · May 1, 2024 KEY POINTS: Lower baseline apparent diffusion coefficient, indicative of greater cortical fibrosis, correlated with higher baseline concentrations of serum markers of inflammation. No association between baseline cortical R2* and baseline serum markers of ... Full text Link to item Cite

Targeting Gut Microbiome With Prebiotic in Patients With CKD: The TarGut-CKD Study.

Journal Article Kidney Int Rep · March 2024 INTRODUCTION: Disruption of gut microbiota underpins some of the metabolic alterations observed in chronic kidney disease (CKD). METHODS: In a nonrandomized, open-label, 3-phase pilot trial, with repeated measures within each phase, we examined the efficac ... Full text Link to item Cite

Practical Considerations and Implementation of Sodium-Glucose Co-Transporter-2 Inhibitors in Chronic Kidney Disease: Who, When, and How? A Position Statement by Nephrologists.

Journal Article J Prim Care Community Health · 2024 INTRODUCTION: There remains an unmet need to reduce kidney and cardiovascular risk in patients with chronic kidney disease (CKD). This report is therefore intended to provide real-world clinical guidance to primary care providers on sodium-glucose co-trans ... Full text Link to item Cite

Kidney and Cardiovascular Effects of Canagliflozin According to Age and Sex: A Post Hoc Analysis of the CREDENCE Randomized Clinical Trial.

Journal Article Am J Kidney Dis · July 2023 RATIONALE & OBJECTIVE: It is unclear whether the effect of canagliflozin on adverse kidney and cardiovascular events in those with diabetic kidney disease varies by age and sex. We assessed the effects of canagliflozin among age group categories and betwee ... Full text Link to item Cite

Study protocol of a randomized controlled trial of fistula vs. graft arteriovenous vascular access in older adults with end-stage kidney disease on hemodialysis: the AV access trial.

Journal Article BMC Nephrol · February 24, 2023 BACKGROUND: Treatment of end-stage kidney disease (ESKD) with hemodialysis requires surgical creation of an arteriovenous (AV) vascular access-fistula (AVF) or graft (AVG)-to avoid (or limit) the use of a central venous catheter (CVC). AVFs have long been ... Full text Link to item Cite

Apixaban for Patients With Atrial Fibrillation on Hemodialysis: A Multicenter Randomized Controlled Trial.

Journal Article Circulation · December 6, 2022 BACKGROUND: There are no randomized data evaluating the safety or efficacy of apixaban for stroke prevention in patients with end-stage kidney disease on hemodialysis and with atrial fibrillation (AF). METHODS: The RENAL-AF trial (Renal Hemodialysis Patien ... Full text Link to item Cite

Abnormalities in Cardiac Structure and Function among Individuals with CKD: The COMBINE Trial.

Journal Article Kidney360 · February 24, 2022 BACKGROUND: Individuals with CKD have a high burden of cardiovascular disease (CVD). Abnormalities in cardiac structure and function represent subclinical CVD and can be assessed by cardiac magnetic resonance imaging (cMRI). METHODS: We investigated differ ... Full text Link to item Cite

Influence of peritoneal dialysis catheter type on complications and long-term outcomes: an updated systematic review and meta-analysis.

Journal Article J Nephrol · December 2021 BACKGROUND: There is currently no consensus regarding the optimal type of peritoneal dialysis catheter (PDC). We compared the outcomes of PDCs according to the number of cuffs, intercuff and intraperitoneal segment shape, and presence of a weighted tip. ME ... Full text Link to item Cite

Surgical versus percutaneous catheter placement for peritoneal dialysis: an updated systematic review and meta-analysis.

Journal Article J Nephrol · October 2021 BACKGROUND: No consensus currently exists regarding the optimal approach for peritoneal dialysis catheter placement. We aimed to compare the outcomes of percutaneous and surgical peritoneal dialysis catheter placement. METHODS: A systematic review of the l ... Full text Link to item Cite

Effect of SGLT2 Inhibitors on Stroke and Atrial Fibrillation in Diabetic Kidney Disease: Results From the CREDENCE Trial and Meta-Analysis.

Journal Article Stroke · May 2021 BACKGROUND AND PURPOSE: Chronic kidney disease with reduced estimated glomerular filtration rate or elevated albuminuria increases risk for ischemic and hemorrhagic stroke. This study assessed the effects of sodium glucose cotransporter 2 inhibitors (SGLT2 ... Full text Open Access Link to item Cite

Kidney Functional Magnetic Resonance Imaging and Change in eGFR in Individuals with CKD.

Journal Article Clin J Am Soc Nephrol · June 8, 2020 BACKGROUND AND OBJECTIVES: Kidney functional magnetic resonance imaging (MRI) requires further investigation to enhance the noninvasive identification of patients at high risk of CKD progression. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: In this explo ... Full text Link to item Cite

A Randomized Trial Comparing the Safety, Adherence, and Pharmacodynamics Profiles of Two Doses of Sodium Bicarbonate in CKD: the BASE Pilot Trial.

Journal Article J Am Soc Nephrol · January 2020 BACKGROUND: Oral sodium bicarbonate (NaHCO3) may preserve kidney function in CKD, even if initiated when serum bicarbonate concentration is normal. Adequately powered trials testing this hypothesis have not been conducted, partly because the best dose for ... Full text Link to item Cite

Effects of Nicotinamide and Lanthanum Carbonate on Serum Phosphate and Fibroblast Growth Factor-23 in CKD: The COMBINE Trial.

Journal Article J Am Soc Nephrol · June 2019 BACKGROUND: Higher serum phosphate and fibroblast growth factor-23 (FGF23) levels may be modifiable to prevent cardiovascular disease in CKD. Short-term studies have reported modest efficacy in phosphate and FGF23 reduction with intestinal phosphate binder ... Full text Link to item Cite

Working Toward an Improved Understanding of Chronic Cardiorenal Syndrome Type 4.

Journal Article Adv Chronic Kidney Dis · September 2018 Chronic diseases of the heart and of the kidneys commonly coexist in individuals. Certainly combined and persistent heart and kidney failure can arise from a common pathologic insult, for example, as a consequence of poorly controlled hypertension or of se ... Full text Link to item Cite

Dialysate Potassium, Dialysate Magnesium, and Hemodialysis Risk.

Journal Article Journal of the American Society of Nephrology : JASN · December 2017 One of the fundamental goals of the hemodialysis prescription is to maintain serum potassium levels within a narrow normal range during both the intradialytic and interdialytic intervals. Considering the extraordinarily high rate of cardiovascular mortalit ... Full text Cite

Serum Potassium Levels and Risk of Sudden Cardiac Death Among Patients With Chronic Kidney Disease and Significant Coronary Artery Disease.

Journal Article Kidney Int Rep · November 2017 INTRODUCTION: Chronic kidney disease (CKD) patients have increased risks of sudden cardiac arrest and sudden cardiac death (SCA/SCD) that are not explained by traditional risk factors. We examined associations between serum potassium and SCA/SCD in a large ... Full text Link to item Cite

Cinacalcet, dialysate calcium concentration, and cardiovascular events in the EVOLVE trial.

Journal Article Hemodial Int · July 2016 Among patients receiving hemodialysis, abnormalities in calcium regulation have been linked to an increased risk of cardiovascular events. Cinacalcet lowers serum calcium concentrations through its effect on parathyroid hormone secretion and has been hypot ... Full text Link to item Cite

Clinical Outcomes in Dialysis Patients: Prospects for Improvement with Aldosterone Receptor Antagonists.

Journal Article Semin Dial · 2016 Patients with advanced chronic kidney disease (CKD), particularly those who have end-stage renal disease (ESRD) and require therapy with dialysis, suffer disproportionately from cardiovascular illnesses. Therapy with mineralocorticoid receptor antagonists ... Full text Link to item Cite

The effect of location and configuration on forearm and upper arm hemodialysis arteriovenous grafts.

Conference J Vasc Surg · November 2015 OBJECTIVE: The arteriovenous graft (AVG) is most often used in hemodialysis patients when an autogenous fistula is not feasible. The optimal location (forearm or upper arm) and configuration (loop or straight) of AVGs are not known. To evaluate relationshi ... Full text Link to item Cite

The Phosphate Binder Ferric Citrate and Mineral Metabolism and Inflammatory Markers in Maintenance Dialysis Patients: Results From Prespecified Analyses of a Randomized Clinical Trial.

Journal Article Am J Kidney Dis · September 2015 BACKGROUND: Phosphate binders are the cornerstone of hyperphosphatemia management in dialysis patients. Ferric citrate is an iron-based oral phosphate binder that effectively lowers serum phosphorus levels. STUDY DESIGN: 52-week, open-label, phase 3, rando ... Full text Link to item Cite

Cinacalcet, Fibroblast Growth Factor-23, and Cardiovascular Disease in Hemodialysis: The Evaluation of Cinacalcet HCl Therapy to Lower Cardiovascular Events (EVOLVE) Trial.

Journal Article Circulation · July 7, 2015 BACKGROUND: Patients with kidney disease have disordered bone and mineral metabolism, including elevated serum concentrations of fibroblast growth factor-23 (FGF23). These elevated concentrations are associated with cardiovascular and all-cause mortality. ... Full text Link to item Cite

Primary prevention implantable cardioverter defibrillators in end-stage kidney disease patients on dialysis: a matched cohort study.

Journal Article Nephrol Dial Transplant · May 2015 BACKGROUND: Sudden cardiac death is the leading cause of death among end-stage kidney disease patients (ESKD) on dialysis, but the benefit of primary prevention implantable cardioverter defibrillators (ICDs) in this population is uncertain. We conducted th ... Full text Link to item Cite

NephSAP: End-Stage Renal Disease and Dialysis

Other NephSAP · November 1, 2014 Cite

Prescriptions for dietary sodium in patients with chronic kidney disease: how will this shake out?

Journal Article Kidney Int · September 2014 Patients with chronic kidney disease (CKD) are at risk of exhibiting expanded extracellular volume, and low-sodium diets are often prescribed to limit clinical complications from this condition. Fan et al. performed a post hoc study from the database of th ... Full text Link to item Cite

Sudden cardiac arrest in ESRD patients.

Journal Article Clin Nephrol · February 2014 End-stage renal disease (ESRD) carries a significant risk for sudden cardiac arrest (SCA), hospitalization and mortality. We present a case of a vintage hemodialysis patient who had a catastrophic event during his hemodialysis treatment - a sudden cardiac ... Full text Link to item Cite

Bardoxolone methyl in type 2 diabetes and stage 4 chronic kidney disease.

Journal Article N Engl J Med · December 26, 2013 BACKGROUND: Although inhibitors of the renin-angiotensin-aldosterone system can slow the progression of diabetic kidney disease, the residual risk is high. Whether nuclear 1 factor (erythroid-derived 2)-related factor 2 activators further reduce this risk ... Full text Link to item Cite

Dialysate calcium concentration and the risk of sudden cardiac arrest in hemodialysis patients.

Journal Article Clin J Am Soc Nephrol · May 2013 BACKGROUND AND OBJECTIVES: The optimal dialysate calcium concentration to maintain normal mineralization and reduce risk of cardiovascular events in hemodialysis patients is debated. Guidelines suggest that dialysate Ca concentration should be lowered to a ... Full text Link to item Cite

IgG4-related tubulointerstitial nephritis associated with chronic lymphocytic leukemia.

Journal Article J Nephrol · 2013 BACKGROUND: Tubulointerstitial nephritis (TIN) is typically seen in association with drug exposure and infection or in autoimmune diseases such as Sjogren's syndrome or systemic lupus erythematosis. The recently described IgG4-related systemic diseases can ... Full text Link to item Cite

Analysis of early hypertension and clinical outcome with bevacizumab: results from seven phase III studies.

Journal Article Oncologist · 2013 BACKGROUND: Hypertension is associated with antivascular endothelial growth factor treatment, but the clinical implications of hypertension are uncertain. To assess the prognostic and predictive value of bevacizumab-related hypertension, a comprehensive an ... Full text Link to item Cite

Prehypertension and chronic kidney disease: the ox or the plow?

Journal Article Kidney Int · February 2012 Nearly ten years ago, practice recommendations supported use of the clinical classification of 'prehypertension' for people with systolic blood pressure of 120-139 mm Hg or diastolic pressure of 80-89 mm Hg. This recommendation was based on observations th ... Full text Link to item Cite

Improving ascertainment of sudden cardiac death in patients with end stage renal disease.

Journal Article Clin J Am Soc Nephrol · January 2012 Featured Publication BACKGROUND AND OBJECTIVES: Data collected by the US Renal Data System (USRDS) identify sudden cardiac death (SCD) as the leading cause of death among hemodialysis patients. However, evidence suggests that clinical events captured on the USRDS death notific ... Full text Link to item Cite

Sudden cardiac death in hemodialysis patients: a comprehensive care approach to reduce risk.

Journal Article Blood Purif · 2012 Sudden cardiac death is a major problem in hemodialysis patients, and our understanding of this disease is underdeveloped. The lack of a precise definition tailored for use in the hemodialysis population limits the reliability of epidemiologic reports. Eff ... Full text Link to item Cite

Clinical trial of focal segmental glomerulosclerosis in children and young adults.

Journal Article Kidney Int · October 2011 Featured Publication This NIH-funded multicenter randomized study of focal segmental glomerulosclerosis (FSGS) treatment compared the efficacy of a 12-month course of cyclosporine to a combination of oral pulse dexamethasone and mycophenolate mofetil in children and adults wit ... Full text Link to item Cite

Remobilization of residual non-aqueous phase liquid in porous media by freeze-thaw cycles.

Journal Article Environ Sci Technol · April 15, 2011 The pore-scale behavior of a nonaqueous phase liquid (NAPL) trapped as residual contamination in a porous medium, subject to freeze-thaw cycles, was investigated by X-ray microcomputed tomography. It is shown that freeze-thaw cycles cause significant NAPL ... Full text Link to item Cite

Clinical trials treating focal segmental glomerulosclerosis should measure patient quality of life.

Journal Article Kidney Int · March 2011 Featured Publication Optimal therapy of patients with steroid-resistant primary focal segmental glomerulosclerosis (FSGS) remains controversial. This report describes the initial study design, baseline characteristics, and quality of life of patients enrolled in the FSGS Clini ... Full text Link to item Cite

Modifiable risk factors associated with sudden cardiac arrest within hemodialysis clinics.

Journal Article Kidney Int · January 2011 Featured Publication Sudden cardiac arrest is the most common cause of death among patients with end-stage kidney disease (ESKD) maintained on hemodialysis. Here we sought to identify dialysis-related factors associated with this increased risk in a case-control study encompas ... Full text Link to item Cite

The authors reply:

Journal Article Kidney International · January 1, 2011 Full text Cite

Study of Heart and Renal Protection (SHARP): randomized trial to assess the effects of lowering low-density lipoprotein cholesterol among 9,438 patients with chronic kidney disease.

Journal Article Am Heart J · November 2010 BACKGROUND: Lowering low-density lipoprotein (LDL) cholesterol with statin therapy has been shown to reduce the incidence of atherosclerotic events in many types of patient, but it remains uncertain whether it is of net benefit among people with chronic ki ... Full text Link to item Cite

Hypertension, chronic kidney disease, and the development of cardiovascular risk: a joint primacy.

Journal Article Kidney Int · May 2010 Featured Publication Patients with chronic kidney disease (CKD) have an inordinate risk of cardiovascular disease. In addition to the metabolic state of uremia, the CKD-associated risk is partly explainable by unique cofactors such as dyslipidemia, systemic inflammation, and e ... Full text Link to item Cite

CHRONIC KIDNEY DISEASE, RISK OF CARDIAC ARREST, AND MYOCARDIAL FIBROSIS

Conference JOURNAL OF INVESTIGATIVE MEDICINE · February 1, 2010 Link to item Cite

CYP3A4 and CYP3A5 polymorphisms and blood pressure response to amlodipine among African-American men and women with early hypertensive renal disease.

Journal Article Am J Nephrol · 2010 Featured Publication PURPOSE: To explore the association between CYP3A4 and CYP3A5 gene polymorphisms and blood pressure response to amlodipine among participants from the African-American Study of Kidney Disease and Hypertension Trial randomized to amlodipine (n = 164). METHO ... Full text Link to item Cite

The authors reply

Journal Article Kidney International · January 1, 2010 Full text Cite

Chronic kidney disease is associated with increased risk of sudden cardiac death among patients with coronary artery disease.

Journal Article Kidney Int · September 2009 Featured Publication Sudden cardiac death is the most common cause of mortality among patients with end-stage kidney disease maintained on hemodialysis. To examine whether this increased risk is also seen with less advanced kidney disease, we studied the relationship between g ... Full text Link to item Cite

G-protein-coupled receptor kinase 4 polymorphisms and blood pressure response to metoprolol among African Americans: sex-specificity and interactions.

Journal Article Am J Hypertens · March 2009 Featured Publication BACKGROUND: African Americans have a disproportionate burden of hypertension and comorbid disease. Pharmacogenetic markers of blood pressure response have yet to be defined clearly. This study explores the association between G-protein-coupled receptor kin ... Full text Link to item Cite

Outcomes of Staphylococcus aureus infection in hemodialysis-dependent patients.

Journal Article Clin J Am Soc Nephrol · February 2009 BACKGROUND AND OBJECTIVES: Staphylococcus aureus is a leading cause of infection in patients with ESRD. Clinical and economic outcomes associated with S. aureus bacteremia and other S. aureus infections in patients with ESRD were examined. DESIGN, SETTING, ... Full text Link to item Cite

A randomized trial of a 6-week course of celecoxib on proteinuria in diabetic kidney disease.

Journal Article Am J Kidney Dis · December 2007 BACKGROUND: Preclinical data suggest that cyclooxygenase 2 inhibitors decrease proteinuria and preserve glomerular structure in animal models of diabetic nephropathy. The objective of this study is to compare the efficacy and safety of celecoxib with place ... Full text Link to item Cite

Angiotensin-converting enzyme gene polymorphism predicts the time-course of blood pressure response to angiotensin converting enzyme inhibition in the AASK trial.

Journal Article J Hypertens · October 2007 OBJECTIVE: It has yet to be determined whether genotyping at the angiotensin-converting enzyme (ACE) locus is predictive of blood pressure response to an ACE inhibitor. METHODS: Participants from the African American Study of Kidney Disease and Hypertensio ... Full text Link to item Cite

Predictors of survival after cardiac arrest in outpatient hemodialysis clinics.

Journal Article Clin J Am Soc Nephrol · May 2007 Featured Publication Cardiac arrest (CA) is the most common cause of death in hemodialysis patients, and factors that improve survival after arrest are unknown. This study sought to identify modifiable factors that are associated with survival after CA in hemodialysis clinics. ... Full text Link to item Cite

Automated external defibrillators and survival from cardiac arrest in the outpatient hemodialysis clinic.

Journal Article J Am Soc Nephrol · January 2007 Featured Publication Automated external defibrillators (AED) have been recommended for use in outpatient dialysis clinics to improve outcomes from cardiac arrest, the most common cause of death in patients with ESRD. The effectiveness of this policy is unknown. The study cohor ... Full text Link to item Cite

Cardiovascular outcomes in the African American Study of Kidney Disease and Hypertension (AASK) Trial.

Journal Article Am J Kidney Dis · November 2006 BACKGROUND: Patients with chronic kidney disease are at increased risk for cardiovascular (CV) events. METHODS: We randomly assigned 1,094 African Americans with hypertensive nephrosclerosis (glomerular filtration rate [GFR], 20 to 65 mL/min/1.73 m(2) [0.3 ... Full text Link to item Cite

Lambda light chain deposition disease in a renal allograft.

Journal Article Transplant Proc · December 2005 Light chain deposition disease (LCDD) of the kidney is characterized by deposition of monoclonal light chains predominantly in glomeruli and in tubular basement membranes. The disease is frequently associated with a lymphoproliferative disorder, and the ma ... Full text Link to item Cite

The relationship between magnitude of proteinuria reduction and risk of end-stage renal disease: results of the African American study of kidney disease and hypertension.

Journal Article Arch Intern Med · April 25, 2005 BACKGROUND: The magnitude of proteinuria is associated with a graded increase in the risk of progression to end-stage renal disease and cardiovascular events. The objective of this study was to relate baseline and early changes in proteinuria and glomerula ... Full text Link to item Cite

Design of the Dialysis Access Consortium (DAC) Clopidogrel Prevention of Early AV Fistula Thrombosis Trial.

Journal Article Clin Trials · 2005 BACKGROUND: The Dialysis Access Consortium (DAC) was developed to investigate interventions to improve hemodialysis vascular access outcomes. The autogenous arteriovenous fistula created by direct connection of native artery to vein is the recommended vasc ... Full text Link to item Cite

Design of the Dialysis Access Consortium (DAC) Aggrenox Prevention Of Access Stenosis Trial.

Journal Article Clin Trials · 2005 BACKGROUND: Surgically created arteriovenous (AV) grafts are the most common type of hemodialysis vascular access in the United States, but fail frequently due to the development of venous stenosis. The Dialysis Access Consortium (DAC) Aggrenox Prevention ... Full text Link to item Cite

The rationale and design of the AASK cohort study.

Journal Article J Am Soc Nephrol · July 2003 Hypertensive kidney disease commonly progresses. The primary objective of the AASK (African American Study of Kidney Disease and Hypertension) Cohort Study is to determine prospectively the course of kidney function and risk factors for kidney disease prog ... Full text Link to item Cite

Preventing renal dysfunction in patients with hypertension: clinical implications from the early AASK Trial results.

Journal Article Am J Cardiovasc Drugs · 2003 Strategies to delay progression of established renal disease have primarily been directed at examining the class of antihypertensive therapy and/or the level of blood pressure control. In diabetic renal disease many trials have noted a protective role of A ... Full text Link to item Cite

Effect of blood pressure lowering and antihypertensive drug class on progression of hypertensive kidney disease: results from the AASK trial.

Journal Article JAMA · November 20, 2002 Featured Publication CONTEXT: Hypertension is a leading cause of end-stage renal disease (ESRD) in the United States, with no known treatment to prevent progressive declines leading to ESRD. OBJECTIVE: To compare the effects of 2 levels of blood pressure (BP) control and 3 ant ... Full text Link to item Cite

Improving survival of octogenarian patients selected for haemodialysis.

Journal Article Nephrol Dial Transplant · November 2001 BACKGROUND: The incidence of end-stage renal disease (ESRD) among patients over the age of 80 has nearly tripled in the last decade, making the 'old-old' the fastest growing ESRD demographic group. Despite this, very little information is available on the ... Full text Link to item Cite

Comparison of cross-sectional renal function measurements in African Americans with hypertensive nephrosclerosis and of primary formulas to estimate glomerular filtration rate.

Journal Article Am J Kidney Dis · October 2001 Renal function measurements were obtained in 1,703 African Americans with presumed hypertensive nephrosclerosis who were screened for entry into the African-American Study of Hypertension and Kidney Disease (AASK). We examined the effect of race on relatio ... Full text Link to item Cite

Effect of ramipril vs amlodipine on renal outcomes in hypertensive nephrosclerosis: a randomized controlled trial.

Journal Article JAMA · June 6, 2001 CONTEXT: Incidence of end-stage renal disease due to hypertension has increased in recent decades, but the optimal strategy for treatment of hypertension to prevent renal failure is unknown, especially among African Americans. OBJECTIVE: To compare the eff ... Full text Link to item Cite

Renal pathology patterns in type II diabetes mellitus: relationship with retinopathy. The Collaborative Study Group.

Journal Article Nephrol Dial Transplant · October 1998 BACKGROUND: The glomerular and retinal vessels are both affected in patients with type I and type II diabetes mellitus. However, the prevalence of the nodular form of diabetic glomerular sclerosis (Kimmelstiel Wilson lesion) and other forms of glomerular p ... Full text Link to item Cite

Ischemic disease of the kidney: how and why to consider revascularization.

Journal Article J Nephrol · 1998 With increasingly accurate non-invasive tests, the clinician frequently discovers obstruction of the renal arteries by atherosclerosis. The decision to reverse this obstruction is not straightforward, particularly when blood pressure can be easily controll ... Link to item Cite

Surgical thrombectomy and revision improves survival of hemodialysis (HD) vascular access

Conference JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY · September 1, 1996 Link to item Cite

Direct regulation of the Na,K pump by signal transduction mechanisms.

Journal Article Miner Electrolyte Metab · 1996 There is clear precedent for a role of signal transduction pathways in modification of the renal sodium pump. Agonists regulate the pump either by changing abundance of subunits, usually chronic processes, or by altering existing transporters. Despite stro ... Link to item Cite

Modulation of thromboxane receptor activation in rat glomerular mesangial cells.

Journal Article Am J Physiol · September 1994 Rat glomerular mesangial cells were used to investigate mechanisms of thromboxane A2 (TxA2) receptor regulation in the kidney. Exposure of mesangial cells to the TxA2 agonist U-46619 for 10 min reduced subsequent TxA2-induced increases in inositol phosphat ... Full text Link to item Cite

Aluminum-induced DNA synthesis in osteoblasts: mediation by a G-protein coupled cation sensing mechanism.

Journal Article J Cell Biochem · September 1994 Aluminum (Al3+) stimulates de novo bone formation in dogs and is a potent stimulus for DNA synthesis in non-transformed osteoblasts in vitro. The recent identification of a G-protein coupled cation-sensing receptor (BoPCaR), which is activated by polyvalen ... Full text Link to item Cite

MODULATION OF THROMBOXANE RECEPTOR ACTIVATION IN RAT GLOMERULAR MESANGIAL CELLS

Journal Article AMERICAN JOURNAL OF PHYSIOLOGY · September 1, 1994 Link to item Cite

DIFFERENTIAL SHORT-TERM REGULATION OF NA/K ATPASE POPULATIONS IN RENAL EPITHELIAL-CELLS

Conference JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY · September 1, 1994 Link to item Cite

Adenosine triphosphate activates ion permeabilities in biliary epithelial cells.

Journal Article Gastroenterology · July 1994 BACKGROUND/AIMS: The biliary epithelium contributes to bile formation through absorption and secretion of fluid and electrolytes. The effects of extracellular nucleotides on membrane ion transport were assessed in isolated bile duct cells from rats and Mz- ... Full text Link to item Cite

Regulation of cation channels in liver cells by intracellular calcium and protein kinase C

Journal Article American Journal of Physiology - Gastrointestinal and Liver Physiology · May 24, 1994 The regulation of Ca2+-permeant cation channels in HTC hepatoma cells was investigated using patch clamp and fluorescence techniques. In intact cells, exposure to nucleotide analogues ATP, uridine 5'-triphosphate (UTP), and adenosine 5'-O-(3-thiotriphospha ... Cite

Regulation of cation channels in liver cells by intracellular calcium and protein kinase C.

Journal Article Am J Physiol · April 1994 The regulation of Ca(2+)-permeant cation channels in HTC hepatoma cells was investigated using patch clamp and fluorescence techniques. In intact cells, exposure to nucleotide analogues ATP, uridine 5'-triphosphate (UTP), and adenosine 5'-O-(3-thiotriphosp ... Full text Link to item Cite

Heterogeneity of protein kinase C-mediated rapid regulation of Na/K-ATPase in kidney epithelial cells.

Journal Article J Biol Chem · July 25, 1993 Na/K-ATPase in renal epithelium is expressed at the basolateral surface and thus is critical for vectorial solute transport. One potential mode of regulation of Na/K-ATPase involves the intracellular effector protein kinase C (PKC). In kidney cell lines, a ... Link to item Cite

Elevation of cAMP is required for down-regulation, but not agonist-induced desensitization, of endogenous dopamine D1 receptors in opossum kidney cells. Studies in cells that stably express a rat cAMP phosphodiesterase (rPDE3) cDNA.

Journal Article J Biol Chem · July 15, 1993 D1 dopamine receptors stimulate cAMP accumulation in opossum kidney (OK) cells, but this response is attenuated by pretreatment with dopamine. Dopamine pretreatment also causes a reduction in D1 dopamine receptor number. We transfected OK cells with a rat ... Link to item Cite

Cl- and K+ transport in human biliary cell lines.

Journal Article Gastroenterology · June 1993 BACKGROUND: The cellular mechanisms which contribute to billing secretion and absorption are not fully defined. The purpose of these studies was to evaluate the membrane ion transport properties of Mz-ChA-1 and Sk-ChA-1 cell lines derived from human biliar ... Full text Link to item Cite

Calcium-dependent regulation of cholecystokinin secretion and potassium currents in STC-1 cells.

Journal Article Am J Physiol · June 1993 Secretory and electrophysiological properties of STC-1 cells, a cholecystokinin-secreting cell line, were examined with a radioimmunoassay and patch-clamp recording techniques. Stimulation of cholecystokinin secretion was seen after exposure to agents anti ... Full text Link to item Cite

Nucleotide receptors regulate membrane ion transport in renal epithelial cells.

Journal Article Am J Physiol · May 1993 Regulation of plasma membrane ion transport by endogenous purinergic receptors was assessed in a distal renal (A6) cell line. Nucleotide analogues stimulated Na-K-Cl cotransport activity with relative potencies of ATP > UTP > ATP gamma S > 2-methylthio-ATP ... Full text Link to item Cite

NUCLEOTIDE RECEPTORS REGULATE MEMBRANE ION-TRANSPORT IN RENAL EPITHELIAL-CELLS

Journal Article AMERICAN JOURNAL OF PHYSIOLOGY · May 1, 1993 Link to item Cite

Prostaglandin F2 alpha-induced mitogenesis in MC3T3-E1 osteoblasts: role of protein kinase-C-mediated tyrosine phosphorylation.

Journal Article Endocrinology · April 1993 Prostaglandin F2 alpha (PGF2 alpha) stimulates DNA synthesis in osteoblasts through phospholipase-C-dependent increases in intracellular calcium and protein kinase-C (PKC) activity. We present evidence that stimulation of protein tyrosine phosphorylation b ... Full text Link to item Cite

Cl- and K+ transport in human biliary cell lines

Journal Article Gastroenterology · 1993 Background: The cellular mechanisms which contribute to billing secretion and absorption are not fully defined. The purpose of these studies was to evaluate the membrane ion transport properties of Mz-ChA-1 and Sk-ChA-1 cell lines derived from human biliar ... Cite

8-hydroxy-2-(di-n-propylamino)tetralin-responsive 5-hydroxytryptamine4-like receptor expressed in bovine pulmonary artery smooth muscle cells.

Journal Article Mol Pharmacol · November 1992 Bovine pulmonary artery smooth muscle (SM) cells express a novel 5-hydroxytryptamine (5-HT) (5-HT4-like) receptor coupled to cAMP accumulation. cAMP radioimmunoassay established the agonist and antagonist profiles of this receptor. 5-HT (EC50 = 91 +/- 33 n ... Link to item Cite

Identification of a regulated Na/K/Cl cotransport system in a distal nephron cell line.

Journal Article Biochim Biophys Acta · October 19, 1992 Lack of an adequate cell model has limited investigation of Na/K/Cl cotransporter regulation in the kidney. Using A6 cells, an amphibian distal renal cell line, we observed that 63% of rubidium uptake in confluent A6 monolayers was ouabain-insensitive. Oua ... Full text Link to item Cite

Functional expression of human 5-HT1A receptors and differential coupling to second messengers in CHO cells.

Journal Article Naunyn Schmiedebergs Arch Pharmacol · August 1992 The signal transduction linkages of the cloned human 5-HT1A receptor as expressed stably in CHO cells were studied. A transfected clonal cell line which expresses 900 +/- 36 fmol 5-HT1A receptor/mg protein (designated CHO-5-HT1A/WT-27) responded to 5-HT an ... Full text Link to item Cite

Fistula dysfunction: effect on rapid hemodialysis.

Journal Article Kidney Int · May 1992 Rapid hemodialysis (Qb 400 to 500 ml/min) places considerable demands on hemodialysis vascular access. This six-month prospective study enrolled 52 patients and evaluated urea recirculation as a means of detecting fistula dysfunction. It evaluated the effe ... Full text Link to item Cite

The alpha 1C-adrenergic receptor: characterization of signal transduction pathways and mammalian tissue heterogeneity.

Journal Article Mol Pharmacol · November 1991 We recently reported the cloning of a novel alpha 1-adrenergic receptor (AR), the alpha 1CAR. By transient and stable expression of the alpha 1CAR and the previously cloned alpha 1BAR in COS-7 and HeLa cells, respectively, we have now compared their abilit ... Link to item Cite

5-HT1A and histamine H1 receptors in HeLa cells stimulate phosphoinositide hydrolysis and phosphate uptake via distinct G protein pools.

Journal Article J Biol Chem · January 5, 1991 Regulation of phosphate uptake was studied in a HeLa cell line after transfection with DNA encoding the human 5-HT1A receptor. In these cells, 5-HT stimulates sodium-dependent phosphate uptake via protein kinase C activation. Endogenous histamine H1 recept ... Link to item Cite

The α1C-adrenergic receptor: Characterization of signal transduction pathways and mammalian tissue heterogeneity

Journal Article Molecular Pharmacology · 1991 We recently reported the cloning of a novel α1-adrenergic receptor (AR), the α1CAR. By transient and stable expression of the α1CAR and the previously cloned α1BAR in COS-7 and HeLa cells, respectively, we have now compared their ability to interact with m ... Cite

The α1C-adrenergic receptor: Characterization of signal transduction pathways and mammalian tissue heterogeneity

Journal Article Molecular Pharmacology · January 1, 1991 We recently reported the cloning of a novel α1-adrenergic receptor (AR), the α1CAR. By transient and stable expression of the α1CAR and the previously cloned α1BAR in COS-7 and HeLa cells, respectively, we have now compared their ability to interact with m ... Cite

Short-term regulation of Na+/K+ adenosine triphosphatase by recombinant human serotonin 5-HT1A receptor expressed in HeLa cells.

Journal Article J Clin Invest · December 1990 Agonist occupancy of the cloned human serotonin (5-HT)1A receptor expressed in HeLa cells stimulates Na+/K+ ATPase activity as assessed by rubidium uptake. The purpose of the study was to determine which of the receptor-associated signaling mechanisms was ... Full text Link to item Cite

Thapsigargin demonstrates calcium-dependent regulation of phosphate uptake in HeLa cells.

Journal Article Am J Physiol · October 1990 We used thapsigargin, a sesquiterpene lactone that mobilizes intracellular Ca without increases in inositol phosphates or major activation of protein kinase C (PKC), to test the specific effects of increasing cytosolic Ca on Na-dependent phosphate uptake i ... Full text Link to item Cite

HeLa cells express cAMP-inhibitable sodium-dependent phosphate uptake.

Journal Article Am J Physiol · February 1990 Receptor-mediated regulation of the sodium-phosphate symporter, and hence sodium-dependent phosphate uptake, typically relates to epithelial cells of renal origin. In this study we have characterized sodium-dependent phosphate uptake and aspects of its rec ... Full text Link to item Cite

HELA-CELLS EXPRESS CAMP-INHIBITABLE SODIUM-DEPENDENT PHOSPHATE-UPTAKE

Journal Article AMERICAN JOURNAL OF PHYSIOLOGY · February 1, 1990 Link to item Cite

The human 5-HT1A receptor expressed in HeLa cells stimulates sodium-dependent phosphate uptake via protein kinase C.

Journal Article J Biol Chem · December 25, 1989 Regulation of phosphate uptake was studied in HeLa cell lines after transfection with DNA encoding the human 5-HT1A receptor. Phosphate uptake was saturable and greater than 90% sodium-dependent, with Vmax approximately 30-35% without changing Km. Treatmen ... Link to item Cite

Protein kinase A, cytosolic calcium, and phosphate uptake in human proximal renal cells.

Journal Article Am J Physiol · October 1989 Phosphate uptake by proximal renal cells derived from the human kidney occurs by a saturable process that is approximately 85% dependent on the presence of sodium. Kinetic analysis is consistent with two distinct transport events with Km of 0.08 and 0.63 m ... Full text Link to item Cite

Hemodialysis-associated subclavian vein stenosis.

Journal Article Kidney Int · June 1988 This study was undertaken to evaluate hemodialysis-associated subclavian vein stenosis (SVS) and to clarify treatment of this condition. Forty-seven patients underwent upper arm venography to evaluate fistula dysfunction. Subclavian vein stenosis was docum ... Full text Link to item Cite

Hepatic biloma complicating sickle cell disease. A case report and a review of the literature.

Journal Article Gastroenterology · April 1984 Previously reported hepatobiliary complications of sickle cell disease include cholelithiasis, intrahepatic cholestasis, cirrhosis, hepatic crisis, abscess, and infarction. We present a case of right upper quadrant pain, fever, and jaundice in a patient wi ... Link to item Cite