Scholars@Duke
Jadee Lee Neff

Jadee Lee Neff

Assistant Professor of Pathology
Pathology
jadee.neff@duke.edu
40 Duke Medicine Circle, DUMC 3712, Durham, NC 27710

Selected Publications

Distinct B/myeloid and T-lymphoblast populations at separate anatomic sites in mixed-phenotype acute leukemia with BCR::ABL1 fusion.

Journal Article J Hematop · September 22, 2025 The pluripotency of malignant blasts in acute leukemias is a growing area of scientific and clinical interest. Mixed-phenotype acute leukemias (MPALs) are defined by the presence of blasts showing evidence of differentiation along at least two lineages. Cu ... Full text Open Access Link to item Cite

Paired single-cell and spatial transcriptional profiling reveals a central osteopontin macrophage response mediating tuberculous granuloma formation.

Journal Article mBio · September 10, 2025 Granulomas are classic manifestations of tuberculosis pathogenesis. They result from an ensemble of immune responses to Mycobacterium tuberculosis infection, but the identities, arrangement, cellular interactions, and regulation of the cells that comprise ... Full text Link to item Cite

Cathepsin Z is a conserved susceptibility factor underlying tuberculosis severity.

Journal Article PLoS Biol · September 2025 Tuberculosis (TB) outcomes vary widely, from asymptomatic infection to mortality, yet most animal models do not recapitulate human phenotypic and genotypic variation. The genetically diverse Collaborative Cross mouse panel models distinct facets of TB dise ... Full text Link to item Cite

Clinicopathologic Features and the Spectrum of Myelokathexis in Warts, Hypogammaglobulinemia, Infections, Myelokathexis Syndrome.

Journal Article Lab Invest · August 2025 Warts, hypogammaglobulinemia, infections, myelokathexis (WHIM) syndrome is a rare primary immunodeficiency disorder predominantly caused by germline CXCR4 variants. Bone marrow (BM) evaluation showing myelokathexis helps to establish the diagnosis of WHIM ... Full text Link to item Cite

56Fe-ion Exposure Increases the Incidence of Lung and Brain Tumors at a Similar Rate in Male and Female Mice.

Journal Article Radiat Res · November 1, 2024 The main deterrent to long-term space travel is the risk of Radiation Exposure Induced Death (REID). The National Aeronautics and Space Administration (NASA) has adopted Permissible Exposure Levels (PELs) to limit the probability of REID to 3% for the risk ... Full text Link to item Cite

Breast cancer exploits neural signaling pathways for bone-to-meninges metastasis.

Journal Article Science · June 21, 2024 The molecular mechanisms that regulate breast cancer cell (BCC) metastasis and proliferation within the leptomeninges (LM) are poorly understood, which limits the development of effective therapies. In this work, we show that BCCs in mice can invade the LM ... Full text Link to item Cite

Tensorial tomographic Fourier ptychography with applications to muscle tissue imaging

Journal Article Advanced Photonics · March 1, 2024 We report tensorial tomographic Fourier ptychography (T2oFu), a nonscanning label-free tomographic microscopy method for simultaneous imaging of quantitative phase and anisotropic specimen information in 3D. Built upon Fourier ptychography, a qu ... Full text Cite

Flow cytometry quantification of tumor-infiltrating lymphocytes to predict the survival of patients with diffuse large B-cell lymphoma.

Journal Article Front Immunol · 2024 INTRODUCTION: Our previous studies have demonstrated that tumor-infiltrating lymphocytes (TILs), including normal B cells, T cells, and natural killer (NK) cells, in diffuse large B-cell lymphoma (DLBCL) have a significantly favorable impact on the clinica ... Full text Open Access Link to item Cite

Dasatinib-associated follicular lymphoma in a patient with B-cell acute lymphoblastic leukaemia.

Journal Article BMJ Case Rep · May 17, 2023 The tyrosine kinase inhibitor dasatinib is approved for the treatment of chronic myeloid leukaemia and Philadelphia chromosome-positive (Ph+) acute lymphoblastic leukaemia (ALL). Patients on dasatinib can rarely develop a form of benign reversible reactive ... Full text Link to item Cite

Acute Myeloid Leukemia, Version 3.2023, NCCN Clinical Practice Guidelines in Oncology.

Journal Article J Natl Compr Canc Netw · May 2023 Acute myeloid leukemia (AML) is a heterogeneous hematologic malignancy characterized by the clonal expansion of myeloid blasts in the peripheral blood, bone marrow, and/or other tissues. It is the most common form of acute leukemia among adults and account ... Full text Link to item Cite

A multiple instance learning approach for detecting COVID-19 in peripheral blood smears.

Journal Article PLOS Digit Health · August 2022 A wide variety of diseases are commonly diagnosed via the visual examination of cell morphology within a peripheral blood smear. For certain diseases, such as COVID-19, morphological impact across the multitude of blood cell types is still poorly understoo ... Full text Open Access Link to item Cite

Sequential Development of JAK2V617F Mutation and BCR-ABL1 Fusion in Individual Patients With Myeloproliferative Neoplasms.

Journal Article Arch Pathol Lab Med · June 1, 2022 CONTEXT.—: Concomitant BCR-ABL1 and JAK2V617F in myeloproliferative neoplasms (MPNs) is rare, and its pathogenesis and clinical significance are unclear. OBJECTIVE.—: To investigate the clonal relationship between the 2 genomic alterations, as well as the ... Full text Link to item Cite

Recommendation on Advanced Molecular Testing in Hematolymphoid Malignancies in the Veterans Population

Conference Blood · November 5, 2021 AbstractThe Veterans Health Administration is the largest integrated provider of cancer services in the nation, with approximately 50,000 new cancer cases diagnosed each year. The goal of the VA's National O ... Full text Cite

Plasma cells are essentially absent in the luminal gastrointestinal tract of patients with "complete" 22q11.2 deletion syndrome (DiGeorge syndrome).

Journal Article Hum Pathol · November 2021 Gastrointestinal symptoms are commonly reported in patients with 22q11.2 deletion syndrome or DiGeorge syndrome (DGS) in addition to the dominant cardiac manifestations and immunodeficiency. But literature providing specific morphologic details of the gast ... Full text Link to item Cite

A Cautionary Tale: Florid Splenic γδ T-cell Proliferation and False-Positive T-cell Clonality by PCR Leads to a Grave Misdiagnosis.

Journal Article Clin Lymphoma Myeloma Leuk · October 2021 The discrimination of benign from malignant lymphoproliferative disorders is sometimes difficult because there can be overlap in their histological and immunophenotypic features. In such situations, molecularly based clonality testing is often used to disc ... Full text Link to item Cite

NCCN Guidelines Insights: Acute Myeloid Leukemia, Version 2.2021.

Journal Article J Natl Compr Canc Netw · January 6, 2021 The NCCN Guidelines for Acute Myeloid Leukemia (AML) provide recommendations for the diagnosis and treatment of adults with AML based on clinical trials that have led to significant improvements in treatment, or have yielded new information regarding facto ... Full text Link to item Cite

Morphologic Leukemia-Free State in Acute Myeloid Leukemia Is Sufficient for Successful Allogeneic Hematopoietic Stem Cell Transplant

Conference Blood · November 5, 2020 Allogeneic hematopoietic cell transplant (HCT) improves survival in patients with relapsed or high risk acute myeloid leukemia (AML). Complete remission (CR) is typically a pre-requisite for transplantation, though many do not achieve a formal CR. ... Full text Cite

Correlation of novel ALKATI with ALK immunohistochemistry and clinical outcomes in metastatic melanoma.

Journal Article Histopathology · October 2020 AIMS: Recently, a novel isoform of anaplastic lymphoma kinase, with alternative transcription initiation (ALKATI ), has been described in melanoma and is susceptible to targeted ALK-inhibitor therapy. Clinical outcomes of patients with ALKATI mutated melan ... Full text Link to item Cite

Histopathologic assessment of cultured human thymus.

Journal Article PLoS One · 2020 The maintenance and propagation of complex mixtures of cells in vitro in the form of native organs or engineered organoids has contributed to understanding mechanisms of cell and organ development and function which can be translated into therapeutic benef ... Full text Open Access Link to item Cite

Myeloid neoplasms in the setting of sickle cell disease: an intrinsic association with the underlying condition rather than a coincidence; report of 4 cases and review of the literature.

Journal Article Mod Pathol · December 2019 Myeloid neoplasms occasionally occur in patients with sickle cell disease, and the underlying connection between the two diseases is unclear. Herein, we retrospectively analyzed four cases of sickle cell disease patients who developed myeloid neoplasm. Age ... Full text Link to item Cite

Sequential development of human herpes virus 8-positive diffuse large B-cell lymphoma and chronic myelomonocytic leukemia in a 59 year old female patient with hemoglobin SC disease.

Journal Article Pathol Res Pract · December 2019 Hematolymphoid neoplasms, including lymphoma and myeloid neoplasms, can occur in patients with sickle cell disease (SCD) or equivalent hemoglobinopathy, but an underlying connection between the two conditions has yet to be fully determined. Herein, we repo ... Full text Link to item Cite

Whole Exome and Transcriptome Sequencing in 1042 Cases Reveals Distinct Clinically Relevant Genetic Subgroups of Follicular Lymphoma.

Journal Article Blood · November 13, 2019 DISCLOSURES: Koff: Burroughs Wellcome Fund: Research Funding; V Foundation: Research Funding; Lymphoma Research Foundation: Research Funding; American Association for Cancer Research: Research Funding. Leppä:Roche: Honoraria, Research Funding; Takeda: Hono ... Full text Link to item Cite

Waldenstrom Macroglobulinemia/Lymphoplasmacytic Lymphoma Concomitant With Non-IgM Plasma Cell Neoplasm: Report of 5 Cases With Laboratory Evidence of Biclonal B-Cell Neoplasms in Single Individuals

Conference American Journal of Clinical Pathology · September 11, 2019 AbstractIntroductionPlasma cell neoplasm (PCN) is rarely associated with Waldenstrom macroglobulinemia (WM)/lymphoplasmacytic lymphoma ... Full text Cite

Mixed-phenotype large granular lymphocytic leukemia: a rare subtype in the large granular lymphocytic leukemia spectrum.

Journal Article Hum Pathol · November 2018 Large granular lymphocytic leukemia (LGLL) is a chronic proliferation of cytotoxic lymphocytes in which more than 70% of patients develop cytopenia(s) requiring therapy. LGLL includes T-cell LGLL and chronic lymphoproliferative disorder of natural killer ( ... Full text Link to item Cite

Development and Verification of an RNA Sequencing (RNA-Seq) Assay for the Detection of Gene Fusions in Tumors.

Journal Article J Mol Diagn · July 2018 We assessed the performance characteristics of an RNA sequencing (RNA-Seq) assay designed to detect gene fusions in 571 genes to help manage patients with cancer. Polyadenylated RNA was converted to cDNA, which was then used to prepare next-generation sequ ... Full text Link to item Cite

Hemophagocytic Lymphohistiocytosis: Overlooked in Critically Ill Adults?

Journal Article Hematological Diseases and Therapies · March 29, 2018 Cite

An Unexpected Finding in SUDEP Involving a Child: Focal Myocardial Infarct Adjacent to Bundle of His.

Journal Article J Forensic Sci · November 2017 Sudden unexpected death in epilepsy (SUDEP) is a nontraumatic, nondrowning death of an individual with epilepsy in which an autopsy with appropriate ancillary studies does not identify a cause of death. The mechanism of death in SUDEP is unknown, but is th ... Full text Link to item Cite

Pediatric Philadelphia-positive B lymphoblastic leukemia with CD56 expression and L2 morphology: Case report and review of the literature

Journal Article Human Pathology Case Reports · June 1, 2017 We present a unique case of pediatric B lymphoblastic leukemia with Philadelphia chromosome, CD56 expression, and predominantly L2 morphology. A review of the current literature demonstrates that this distinct combination of features is rare and may porten ... Full text Cite

Distinguishing T-cell large granular lymphocytic leukemia from reactive conditions. Laboratory tools and challenges in their use.

Journal Article Surgical Pathology Clinics · December 1, 2013 This article focuses on the challenges of diagnosing T-cell large granular leukemia and distinguishing it from benign reactive conditions, as well as more aggressive neoplasms ofcytotoxic lymphocytes. No single laboratory method is sufficient to make the d ... Full text Cite

Helicobacter canis bacteremia in a patient with fever of unknown origin.

Journal Article J Clin Microbiol · March 2013 A 57-year-old woman with common variable immune deficiency and liver failure of unknown etiology presented with recurrent fevers over a 5-month period. She was found to have Helicobacter canis bacteremia. Immunocompromised hosts with exposure to cats or do ... Full text Link to item Cite

2B4 utilizes ITAM-containing receptor complexes to initiate intracellular signaling and cytolysis.

Journal Article Mol Immunol · May 2011 2B4 is a member of the SLAM receptor family capable of activating NK cell cytotoxicity in the context of EBV infection. SAP (SLAM Associated Protein) deficiency causes defective signaling downstream of SLAM family receptors and high susceptibility to EBV. ... Full text Link to item Cite

TREM-2 mediated signaling induces antigen uptake and retention in mature myeloid dendritic cells.

Journal Article J Immunol · December 1, 2008 Myeloid dendritic cells (mDC) activated with a B7-DC-specific cross-linking IgM Ab (B7-DC XAb) take up and retain Ag and interact with T cell compartments to affect a number of biologic changes that together cause strong antitumor responses and blockade of ... Full text Link to item Cite

NKG2D-mediated activation of cytotoxic lymphocytes: unique signaling pathways and distinct functional outcomes.

Journal Article Semin Immunol · June 2006 NKG2D is an important immunosurveillance receptor that triggers a unique signal transduction pathway resulting in a variety of functional outcomes. NKG2D couples to the non-ITAM-containing DAP10 and initiates at least two signaling branches that are both r ... Full text Link to item Cite

NKG2D-mediated signaling requires a DAP10-bound Grb2-Vav1 intermediate and phosphatidylinositol-3-kinase in human natural killer cells.

Journal Article Nat Immunol · May 2006 NKG2D is an important immunosurveillance receptor that responds to stress-induced ligand expression on tumors and virus-infected cells. Human natural killer cells express NKG2D and require the transmembrane adaptor DAP10 to initiate their full cytotoxic ac ... Full text Link to item Cite

The isoforms of phospholipase C-gamma are differentially used by distinct human NK activating receptors.

Journal Article J Immunol · July 1, 2005 The two isoforms of phospholipase C (PLC)-gamma couple immune recognition receptors to important calcium- and protein kinase C-dependent cellular functions. It has been assumed that PLC-gamma1 and PLC-gamma2 have redundant functions and that the receptors ... Full text Link to item Cite

NKG2D-DAP10 triggers human NK cell-mediated killing via a Syk-independent regulatory pathway.

Journal Article Nat Immunol · June 2003 The immune recognition receptor complex NKG2D-DAP10 on natural killer cells is stimulated by specific ligands carried on virus-infected and malignant cells. Because DAP10 does not have an immunoreceptor tyrosine-based activation motif (ITAM) in its cytopla ... Full text Link to item Cite

Preservation of motor function by inhibition of CD8+ virus peptide-specific T cells in Theiler's virus infection.

Journal Article FASEB J · December 2001 Central nervous system-infiltrating CD8+ T cells are potential mediators of neuropathology in models of multiple sclerosis induced by Theiler's murine encephalomyelitis virus (TMEV) infection. C57BL/6 mice mount a vigorous cytotoxic T lymphocyte (CTL) resp ... Full text Link to item Cite

Interconversion of different crystal forms of Fabs from human IgM cryoglobulins

Journal Article Journal of Crystal Growth · November 1, 2001 In attempts to produce diffraction-quality crystals of Fabs from two human IgM cryoglobulins (Pot and Yvo), we observed unexpected interconversions in crystal morphologies. The Pot Fab crystallized in two forms when polyethylene glycol (PEG) 6,000 was used ... Full text Cite