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John Matthew Velkey

Assistant Professor of the Practice of Medical Education in Cell Biology
Cell Biology

Selected Publications

Body and Brain: Anatomy of team-based learning in a preclinical science course

Conference AMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY · March 1, 2015 Link to item Cite

Expression of Neurogenin 1 in Mouse Embryonic Stem Cells Directs the Differentiation of Neuronal Precursors and Identifies Unique Patterns of Down-stream Gene Expression.

Journal Article Dev Dyn · May 2013 COVER PHOTOGRAPH: Neuronal differentiation of tet-inducible Neurogenin1-expressing ES cells 3 days after transgene induction, stained with antibodies recognizing Sox3 (green) and neuron-specific class III b-tubulin (amber); nuclei stained with Hoechst 3325 ... Full text Link to item Cite

Expression of Neurogenin 1 in mouse embryonic stem cells directs the differentiation of neuronal precursors and identifies unique patterns of down-stream gene expression.

Journal Article Dev Dyn · March 2013 BACKGROUND: Delineating the cascades of growth and transcription factor expression that shape the developing nervous system will improve our understanding of its molecular histogenesis and suggest strategies for cell replacement therapies. In the current i ... Full text Link to item Cite

BDNF profoundly and specifically increases KCNQ4 expression in neurons derived from embryonic stem cells.

Journal Article Stem Cell Res · January 2013 Neurons resembling the spiral ganglion neurons (SGNs) of the auditory nerve can be generated from embryonic stem cells through induced overexpression of the transcription factor Neurogenin-1 (Neurog1). While recapitulating this developmental pathway produc ... Full text Link to item Cite

Combining topographical and genetic cues to promote neuronal fate specification in stem cells.

Journal Article Biomacromolecules · November 12, 2012 There is little remedy for the devastating effects resulting from neuronal loss caused by neural injury or neurodegenerative disease. Reconstruction of damaged neural circuitry with stem cell-derived neurons is a promising approach to repair these defects, ... Full text Link to item Cite

Netrin-1-mediated axon guidance in mouse embryonic stem cells overexpressing neurogenin-1.

Journal Article Stem Cells Dev · October 10, 2012 Stem cell therapy holds great promise for treating neurodegenerative disease, but major barriers to effective therapeutic strategies remain. A complete understanding of the derived phenotype is required for predicting cell response once introduced into the ... Full text Link to item Cite

Intraspinal transplantation of neurogenin-expressing stem cells generates spinal cord neural progenitors.

Journal Article Neurobiol Dis · April 2012 Embryonic stem (ES) cells and their derivatives are an important resource for developing novel cellular therapies for disease. Controlling proliferation and lineage selection, however, are essential to circumvent the possibility of tumor formation and faci ... Full text Link to item Cite

Glutamatergic neuronal differentiation of mouse embryonic stem cells after transient expression of neurogenin 1 and treatment with BDNF and GDNF: in vitro and in vivo studies.

Journal Article J Neurosci · November 26, 2008 Differentiation of the pluripotent neuroepithelium into neurons and glia is accomplished by the interaction of growth factors and cell-type restricted transcription factors. One approach to obtaining a particular neuronal phenotype is by recapitulating the ... Full text Link to item Cite

Manipulation of OCT4 levels in human embryonic stem cells results in induction of differential cell types.

Journal Article Exp Biol Med (Maywood) · November 2007 To fully understand self-renewal and pluripotency and their regulation in human embryonic stem cells (hESCs), it is necessary to generate genetically modified cells and analyze the consequences of elevated and reduced expression of genes. Genes expressed i ... Full text Link to item Cite

Gene silencing using RNA interference in embryonic stem cells.

Journal Article Methods Mol Biol · 2006 Pluripotent embryonic stem (ES) cells are an important model system to examine gene expression and lineage segregation during differentiation. One powerful approach to target and inhibit gene expression, RNAi, has been applied to ES cells with the goal of ... Full text Link to item Cite

Oct4 RNA interference induces trophectoderm differentiation in mouse embryonic stem cells.

Journal Article Genesis · September 2003 We examined whether suppression of Oct4 via RNA interference (RNAi) would affect embryonic stem (ES) cell lineage choice. Cells were transfected with plasmids containing an independently expressed reporter gene and an RNA polymerase type III promoter to co ... Full text Link to item Cite

Development of the coronary blood supply: changing concepts and current ideas.

Journal Article Anat Rec · August 15, 2002 Earlier views of the development of the coronary vasculature included angiogenic budding and growth of arteries from the aortic sinuses and veins from the coronary sinus. The current concept begins with the establishment of the epicardium from the proepica ... Full text Link to item Cite

Apoptosis during coronary artery orifice development in the chick embryo.

Journal Article Anat Rec · March 1, 2001 Previous studies regarding the development of proximal segments of the coronary arteries in the chick have demonstrated that these vessels do not develop as angiogenic outgrowths from the aorta. Rather, the proximal segments of the coronary arteries arise ... Full text Link to item Cite