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Patrick Joseph Buckley

Patrick Joseph Buckley

Professor Emeritus of Pathology
Pathology
patrick.buckley@duke.edu
Duke Box 3712, Durham, NC 27710
M227 Davison Bldg, Durham, NC 27710
Office hours By arrangement  

Selected Publications

Favorable Trisomies and ETV6-RUNX1 Predict Cure in Low-Risk B-Cell Acute Lymphoblastic Leukemia: Results From Children's Oncology Group Trial AALL0331.

Journal Article J Clin Oncol · May 10, 2021 PURPOSE: Children's Oncology Group (COG) AALL0331 tested whether pegaspargase intensification on a low-intensity chemotherapy backbone would improve the continuous complete remission (CCR) rate in a low-risk subset of children with standard-risk B-acute ly ... Full text Link to item Cite

Outcome in Children With Standard-Risk B-Cell Acute Lymphoblastic Leukemia: Results of Children's Oncology Group Trial AALL0331.

Journal Article J Clin Oncol · February 20, 2020 PURPOSE: Children's Oncology Group (COG) AALL0331 tested whether intensified postinduction therapy that improves survival in children with high-risk B-cell acute lymphoblastic leukemia (ALL) would also improve outcomes for those with standard-risk (SR) ALL ... Full text Link to item Cite

What are the pathology education requirements for all nonpathology ACGME-accredited programs in an academic center?

Journal Article Am J Clin Pathol · September 2012 This study aimed to determine institution-wide graduate medical education (GME) requirements in pathology (exclusive of pathology residency and fellowships) at an academic center. All documents related to residency review committee (RRC) program requiremen ... Full text Link to item Cite

Escalating intravenous methotrexate improves event-free survival in children with standard-risk acute lymphoblastic leukemia: a report from the Children's Oncology Group.

Journal Article Blood · July 14, 2011 Featured Publication Children's Cancer Group-1991 selected 2 components from the Children's Cancer Group studies shown to be effective in high-risk acute lymphoblastic leukemia and examined them in children with National Cancer Institute standard-risk acute B-precursor lymphob ... Full text Link to item Cite

Donor cell-derived leukemias/myelodysplastic neoplasms in allogeneic hematopoietic stem cell transplant recipients: a clinicopathologic study of 10 cases and a comprehensive review of the literature.

Journal Article Am J Clin Pathol · April 2011 We report 10 cases of donor cell leukemia (DCL). All cases except the case of chronic lymphocytic leukemia had anemia, neutropenia, and/or thrombocytopenia when DCL was diagnosed. Eight cases with sex-mismatched hematopoietic stem cell transplant (HCT) sho ... Full text Link to item Cite

Distinct expression patterns of CD123 and CD34 on normal bone marrow B-cell precursors ("hematogones") and B lymphoblastic leukemia blasts.

Journal Article Am J Clin Pathol · October 2009 We compared the expression of CD123, the alpha chain of the interleukin-3 receptor, on normal B-cell precursors in bone marrow ("hematogones") from 75 specimens and on leukemic blasts in 45 newly diagnosed B-acute lymphoblastic leukemias (B-ALL) cases. We ... Full text Link to item Cite

Escalating Dose Intravenous Methotrexate without Leucovorin Rescue during Interim Maintenance Is Superior to Oral Methotrexate for Children with Standard Risk Acute Lymphoblastic Leukemia (SR-ALL): Children’s Oncology Group Study 1991

Journal Article Blood · November 16, 2008 AbstractA substantial number of relapses derive from the NCI standard risk population. CCG-1991 tested two components of the successful COG augmented “BFM” regimen (N Engl J Med1998; 338:1663) in this subset ... Full text Cite

OPPOSING PATTERNS OF CD123/CD34 EXPRESSION IN HEMATOGONES AND PRECURSOR B-ALL BLASTS.

Conference CYTOMETRY PART B-CLINICAL CYTOMETRY · November 1, 2008 Link to item Cite

Safety and efficacy of gemtuzumab ozogamicin in combination with chemotherapy for pediatric acute myeloid leukemia: a report from the Children's Oncology Group.

Journal Article J Clin Oncol · May 10, 2008 PURPOSE: While gemtuzumab ozogamicin (GTMZ) is commonly used in the treatment of acute myeloid leukemia (AML) in combination with standard chemotherapy agents, the pediatric maximum-tolerated dose (MTD) of GMTZ in combination with chemotherapy has not been ... Full text Link to item Cite

Clinicopathologic findings in high-grade B-cell lymphomas with typical Burkitt morphologic features but lacking the MYC translocation.

Journal Article Am J Clin Pathol · December 2007 In the World Health Organization classification, cases with classical Burkitt morphologic features and a very high proliferation fraction but without the MYC translocation are not clearly designated as a separate entity and are usually categorized as diffu ... Full text Link to item Cite

Morphologic examination of sequential bone marrow biopsies after nonmyeloablative stem cell transplantation complements molecular studies of donor engraftment.

Journal Article Arch Pathol Lab Med · October 2006 CONTEXT: Nonmyeloablative stem cell transplantation (NMSCT) is a mode of immunotherapy increasingly employed in treating hematologic, lymphoid, and solid tumors. Patients are monitored principally by molecular analysis of donor engraftment. OBJECTIVE: To d ... Full text Link to item Cite

Posttransplant lymphoproliferative disorder after umbilical cord blood transplantation in children

Journal Article American Journal of Surgical Pathology · 2006 Reported are 7 cases of posttransplant lymphoproliferative disorder (PTLD) arising in children who received umbilical cord blood transplantation (UCBT). There were 4 females and 3 males with a median age of 3 years (range, 1-16 years). All 7 patients recei ... Full text Cite

The value of fluorescence in situ hybridization and polymerase chain reaction in the diagnosis of B-cell non-Hodgkin lymphoma by fine-needle aspiration.

Journal Article Arch Pathol Lab Med · December 2004 CONTEXT: Molecular genetic analyses have been predicted to improve the diagnostic accuracy of fine-needle aspiration of B-cell non-Hodgkin lymphoma. OBJECTIVE: To determine the value of routine molecular genetic assays, polymerase chain reaction (PCR) and ... Full text Link to item Cite

Dose-intense cyclophosphamide and etoposide for patients with refractory or high-risk non-Hodgkin's lymphoma.

Journal Article Clin Lymphoma · September 2004 A retrospective review was performed on the toxicity and response to one cycle of dose-intense cyclophosphamide/etoposide, followed by consolidation in patients with refractory or previously untreated, high-risk non-Hodgkin's lymphoma (NHL). Fifty-five pat ... Full text Link to item Cite

Diagnostic impact of core-needle biopsy on fine-needle aspiration of non-Hodgkin lymphoma.

Journal Article Diagn Cytopathol · July 2004 Featured Publication We retrospectively reviewed 74 fine-needle aspiration (FNA) cases of presumptive non-Hodgkin lymphoma (NHL). All the cases had cytology and core-needle biopsy and 53 cases had concurrent flow cytometric analysis. FNA (cytology and flow cytometry) and core- ... Full text Link to item Cite

Posttransplant lymphoproliferative disorder following nonmyeloablative allogeneic stem cell transplantation.

Journal Article Am J Surg Pathol · June 2004 Posttransplantation lymphoproliferative disorder (PTLD) is a well-recognized complication of conventional bone marrow/stem cell and solid organ transplantation. However, not much is known about PTLD following the more recently introduced nonmyeloablative a ... Full text Link to item Cite

Cytogenetic and molecular analysis of an unusual case of acute promyelocytic leukemia with a t(15;17;17)(q22;q23;q21).

Journal Article Cancer Genet Cytogenet · August 2003 Featured Publication We present a 52-year-old female with a clinical history of acute myelocytic leukemia, probable acute promyelocytic leukemia (APL). Flow cytometry results were somewhat unusual. Specifically, the promyelocytic population showed partial positivity for antige ... Full text Link to item Cite

Burkitt lymphoma arising in organ transplant recipients: a clinicopathologic study of five cases.

Journal Article Am J Surg Pathol · June 2003 We report five cases of Burkitt lymphoma arising in organ transplant recipients. There were four men and one woman with a mean age of 35 years. All were solid organ recipients with three renal, one liver, and one double lung transplantation. The time inter ... Full text Link to item Cite

Value of CD23 determination by flow cytometry in differentiating mantle cell lymphoma from chronic lymphocytic leukemia/small lymphocytic lymphoma.

Journal Article Am J Clin Pathol · December 2001 Featured Publication Mantle cell lymphoma (MCL) and chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) share many morphologic and immunophenotypic features. In addition to histomorphologic examination, it is customary to use the absence of CD23 to differentiate ... Full text Link to item Cite

Increased glomerular deposits of von Willebrand factor in chronic, but not acute, rejection of primate renal allografts.

Journal Article Transplantation · September 27, 2000 Featured Publication BACKGROUND: In our previously described primate renal allograft model, T cell ablation leads to long-term graft survival. The role of endothelial cell alteration in chronic rejection was examined in our model. METHODS: Renal transplants were performed in r ... Full text Link to item Cite

Neutrophil gelatinase-associated lipocalin in normal and neoplastic human tissues. Cell type-specific pattern of expression.

Journal Article Histochem J · July 1999 Neutrophil gelatinase-associated lipocalin (NGAL) has recently been identified in myeloperoxidase-negative neutrophil granules. Members of the lipocalin family are thought to bind and transport small lipophilic molecules such as retinoids and roles in cell ... Full text Link to item Cite

Primate renal transplants using immunotoxin.

Journal Article Surgery · August 1998 BACKGROUND: T-lymphocyte depletion 7 days before transplantation with immunotoxin FN 18-CRM9 has resulted in tolerance to subsequent renal allografts. We tested the effect of giving immunotoxin on the day of the transplantation and evaluated its effect on ... Link to item Cite

Analysis of primate renal allografts after T-cell depletion with anti-CD3-CRM9.

Journal Article Transplantation · July 15, 1998 BACKGROUND: FN18-CRM9 is a CD3-specific immunotoxin that is capable of depleting CD3+ T cells. Pretreatment of rhesus monkeys with this agent before transplantation can induce donor-specific tolerance and "split tolerance" to renal allografts. METHODS: Het ... Full text Link to item Cite

Alteration of the CD34+ Tf-1 beta cell line profile in response to long-term exposure to IL-15.

Journal Article Cytokine · May 1997 Interleukin 15 (IL-15) is a cytokine with many functional characteristics that are similar to IL-2. Most of the functional activities that IL-2 and IL-15 support have been evaluated in short-term assays. It was our intention, then, to determine the long-te ... Full text Link to item Cite

Secretion of a large molecular-weight form of insulin-like growth factor by a primary renal tumor.

Journal Article Med Pediatr Oncol · June 1995 A 5-year-old boy with an abdominal mass was found to have a primary renal tumor of poorly identifiable histology. Prior to resection of the tumor, the patient exhibited several episodes of biochemical hypoglycemia. The hypoglycemia did not recur after oper ... Full text Link to item Cite

Lymphoproliferative disease of granular lymphocytes.

Journal Article J Am Acad Dermatol · May 1995 We report a case of lymphoproliferative disease of granular lymphocytes that was associated with cutaneous manifestations. Marker studies of skin and blood in this patient revealed that the circulating and infiltrating cells were negative for CD3 and T-cel ... Full text Link to item Cite

Massive orbital teratoma in the newborn.

Journal Article Ann Plast Surg · November 1993 Featured Publication Teratomas are rare germ cell tumors that comprise approximately 1% of orbital tumors in childhood. Review of the world literature revealed only 51 well-documented patients with true congenital orbital teratomas. We present a newborn girl with a massive orb ... Full text Link to item Cite

Diagnosis of gastrointestinal T-cell lymphomas in routinely processed tissues.

Journal Article J Clin Gastroenterol · July 1993 Featured Publication Diagnosis of primary gastrointestinal T-cell lymphomas is often problematic because lymphoma may not be suspected clinically or on resection, and tissue may not be frozen for immunophenotyping. Furthermore, ulceration and inflammation and the polymorphous ... Full text Link to item Cite

Idiopathic retroperitoneal fibrosis is a macrophage-rich process. Implications for its pathogenesis and treatment.

Journal Article Am J Surg Pathol · May 1993 Featured Publication Idiopathic retroperitoneal fibrosis (IRF) is an uncommon disease of obscure etiology and pathobiology. Using sections of frozen and paraffin-embedded tissue, an immunohistochemical technique, and antibodies to a variety of macrophage- and lymphocyte-associ ... Full text Link to item Cite

Macrophages (histiocytes) in various reactive and inflammatory conditions express different antigenic phenotypes.

Journal Article Hum Pathol · December 1992 The purpose of this study was to determine whether human tissue macrophages (M phi s) in various inflammatory/reactive conditions express different immunophenotypes. Using a large panel of monoclonal antibodies to monocyte/M phi-related antigens and a froz ... Full text Link to item Cite

Mantle zone lymphoma in a gastric glomus tumor.

Journal Article Cancer · November 1, 1992 Featured Publication A 61-year-old man had an ileocolectomy for resection of an obstructing lesion of the terminal ileum, which proved to be a mantle zone variant of intermediate lymphocytic lymphoma. At laparotomy, an intramural nodule in the gastric antrum was observed; on r ... Full text Link to item Cite

Morphologic, biochemical, and cytogenetic studies of bone marrow and circulating blood cells in painters exposed to ethylene glycol ethers.

Journal Article Environ Res · October 1992 In a previous cross-sectional survey, up to 15% of shipyard painters were found to have mild anemia or granulocytopenia, mostly acquired since employment. Environmental studies had suggested a possible etiologic role for ethylene glycol ethers, solvents to ... Full text Link to item Cite

Correlations of unique clinical, immunotypic, and histologic findings in cutaneous gamma/delta T-cell lymphoma.

Journal Article J Am Acad Dermatol · May 1992 Cutaneous T-cell lymphoma is a malignancy of T cells that express a clone-specific heterodimer T-cell receptor for antigen. The second recognized case of an epidermotropic malignancy of T-cells expressing gamma/delta T-cell receptor-expressing cells is rep ... Full text Link to item Cite

Histiocytes in familial and infection-induced/idiopathic hemophagocytic syndromes may exhibit phenotypic differences.

Journal Article Pediatr Pathol · 1992 Featured Publication Familial hemophagocytic syndrome (FHS) and infection-associated hemophagocytic syndrome (IAHS) usually present with fever, pancytopenia, hepatosplenomegaly, signs of hepatic dysfunction, bleeding diathesis, and neurological manifestations. FHS is almost un ... Full text Link to item Cite

Phenotypic subpopulations of macrophages and dendritic cells in human spleen.

Journal Article Scanning Microsc · March 1991 Featured Publication Using immunohistochemical techniques and a large number of monoclonal antibodies, the presence and distribution of phenotypic subpopulations of macrophages (MOs) and dendritic cells in human spleen were assessed. The results of this study show that differe ... Link to item Cite

Infection of T but not B lymphocytes by cytomegalovirus in lymph node. An immunophenotypic study.

Journal Article Am J Surg Pathol · January 1991 A 45-year-old black man presented with fever, night sweats, weight loss, and cervical lymphadenopathy. Biopsy of an enlarged node showed partial effacement of the nodal architecture by numerous cells with typical cytomegalovirus (CMV) inclusions. These wer ... Full text Link to item Cite

Partial splenectomy for symptomatic splenic hamartoma.

Journal Article J Pediatr Surg · December 1990 Lower pole splenectomy with preservation of residual splenic function was successfully performed in a 3-year-old boy with a symptomatic splenic hamartoma. Following resection, several of the patient's constitutional symptoms resolved. This is the first rep ... Full text Link to item Cite

Primary tumor of spleen with morphologic features of malignant fibrous histiocytoma. Immunohistochemical evidence for a macrophage origin.

Journal Article Am J Surg Pathol · November 1990 Featured Publication We report a case of primary splenic malignant fibrous histiocytoma that occurred in a 41-year-old man. Adjacent to the tumor there was a large calcified intrasplenic cyst. Despite splenectomy, postoperative radiation, and systemic chemotherapy, the patient ... Full text Link to item Cite

Immunophenotypic characterization of sinus histiocytosis with massive lymphadenopathy (Rosai-Dorfman disease).

Journal Article Semin Diagn Pathol · February 1990 Featured Publication Histochemical and immunohistochemical studies have been reported in only a few cases of sinus histiocytosis with massive lymphadenopathy (SHML) to date. These indicate that SHML cells belong to the macrophage/histiocyte family, but their exact origin is st ... Link to item Cite

Elevated soluble interleukin-2 receptor in childhood hemophagocytic histiocytic syndromes.

Journal Article Blood · June 1989 The serum of children with untreated hemophagocytic syndromes contains elevated levels (23,600 to 75,200 U/mL) of soluble interleukin-2 receptor (SIL2R) that returns toward normal with clinical improvement. These levels are in excess of levels previously r ... Link to item Cite

The phenotype of human placental macrophages and its variation with gestational age.

Journal Article Am J Pathol · December 1988 The antigenic phenotype of human villous stromal macrophages (M phi s) from first and third trimester placentas was analyzed using a large number of monoclonal antibodies (MAbs) to monocyte (Mo)/M phi-associated cell membrane determinants. The purpose of t ... Link to item Cite

Sonographic tissue pattern in neuroblastoma.

Journal Article AJR Am J Roentgenol · March 1988 Featured Publication Full text Link to item Cite

The immunologic approach to analysis of malignant lymphoma. Mantle zone lymphoma of the ileocecal region.

Journal Article J Clin Gastroenterol · October 1987 Featured Publication We studied an unusual type of lymphoma of the ileocecal region using an established protocol that combines morphologic, flow cytometric, and immunohistochemical analyses. This lymphoma grew as multiple submucosal nodules, some of which had coalesced into a ... Full text Link to item Cite

Human spleen contains phenotypic subsets of macrophages and dendritic cells that occupy discrete microanatomic locations.

Journal Article Am J Pathol · September 1987 Featured Publication Macrophages (M phi s) are an important component of the immune response and mediate numerous other functions. Phenotypic and functional subsets of circulating monocytes have been described, but few similar studies have analyzed M phi s in human tissues. By ... Link to item Cite

Thymoma and myasthenia gravis in a 4-year-old child. Case report and review of the literature.

Journal Article Cancer · December 1, 1985 Featured Publication Thymomas are exceedingly rare in the first 20 years of life, and only a few well-documented cases are present in the literature. Thymomas in adults are commonly associated with other diseases, the most frequent being myasthenia gravis (MG). However, this a ... Full text Link to item Cite

Human splenic sinusoidal lining cells express antigens associated with monocytes, macrophages, endothelial cells, and T lymphocytes.

Journal Article J Immunol · April 1985 Featured Publication The antigenic and functional properties of splenic sinusoidal lining cells (SLC) have not been studied extensively. Some investigators have suggested that SLC are actively phagocytic, and thus part of the mononuclear phagocyte system. Others dispute this a ... Link to item Cite

Developmental cystic renal neoplasms in children. Diagnostic imaging characteristics.

Journal Article Comput Radiol · 1985 Featured Publication We report diagnostic imaging and histopathologic findings in four children with different cystic renal neoplasms. The features discerned with computed tomography (CT), as well as ultrasound, correlated well with gross histologic findings, although a defini ... Full text Link to item Cite

Monoclonal antibodies to T-helper/inducer and T-suppressor/cytotoxic lymphocyte subsets recognize antigens on splenic sinusoidal lining cells.

Journal Article Am J Clin Pathol · August 1984 Featured Publication Most of the monoclonal antibodies (MoAbs) to T-lymphocyte subsets are, with few exceptions thought to recognize antigens (Ags) unique to T-cells. The authors examined the distribution of cells reacting with MoAbs to the helper/inducer (OKT4, anti-Leu-3a,b) ... Full text Link to item Cite

Some fixation reagents reduce or abolish the detectability of Ia-antigen and HLA-DR on cells.

Journal Article J Immunol Methods · February 24, 1984 Featured Publication The effect of paraformaldehyde (PF), glutaraldehyde (GT), methanol (ME), ethanol (ET) and acetone (AC) fixation on the detectability of Ia antigens on murine and rat peritoneal exudate (PE) and resident peritoneal (RP) macrophages (M phi), and on detectabi ... Full text Link to item Cite

Isolation of human splenic macrophages and lymphocytes by countercurrent centrifugal elutriation.

Journal Article J Immunol Methods · February 10, 1984 Featured Publication Certain tissues, such as the spleen, are rich sources of mononuclear phagocytes (MP); however, separating the phagocytes from tissues and removing the contaminating cells have been difficult. We report here a method for the extraction and purification of h ... Full text Link to item Cite

Acromegaly and Cushing's syndrome associated with a foregut carcinoid tumor.

Journal Article J Clin Endocrinol Metab · October 1981 Featured Publication We report an 18-yr-old youth with a metastatic foregut carcinoid tumor, Cushing's syndrome, and hypersomatotropic gigantism. Administration of cyproheptadine caused a dramatic fall in urinary cortisol excretion and plasma ACTH levels associated with clinic ... Full text Link to item Cite

Fatal cytomegalovirus bronchiolitis in a patient with Nezelof's syndrome.

Journal Article Pediatrics · January 1980 Featured Publication A 4-year-old girl who had received a fetal thymus gland by intraperitoneal transplantation 41 months previously sustained acute, fatal bronchiolitis due to culture-proven cytomegalovirus despite the fact that a specific antibody response to this organism w ... Link to item Cite

Multiple and specific initiation of T4 DNA replication.

Journal Article J Virol · July 1973 Featured Publication Partially replicated T4 DNA molecules (PRM) whose parental or progeny DNA was labeled with bromodeoxyuridine BUdR was analyzed by gradual shearing followed by CsCl banding of the sheared product. Analysis of PRM containing 18-mum replicated DNA showed that ... Full text Link to item Cite

In vivo production of an RNA-DNA copolymer after infection of Escherichia coli by bacteriophage T4.

Journal Article Proc Natl Acad Sci U S A · November 1972 Featured Publication An RNA-DNA copolymer was isolated from Escherichia coli infected with bacteriophage T4. The RNA and DNA are covalently linked, and in the same polynucleotide strand. The DNA of the copolymer hybridizes specifically to the left strand of phage T4 DNA. The c ... Full text Link to item Cite

Early intracellular events in the replication of bacteriophage T4 deoxyribonucleic acid. VI. Newly synthesized proteins in the T4 protein-deoxyribonucleic acid complex.

Journal Article J Virol · April 1970 Shortly after infection of Escherichia coli B with T4 phage, the phage deoxyribonucleic acid (DNA) can be isolated as a fast-sedimenting, proteinaceous complex. Formation of the complex is inhibited by the addition of chloramphenicol between 3 and 4 min af ... Full text Link to item Cite

Pigment production from tryptophan by an Achromobacter species.

Journal Article J Bacteriol · December 1965 Featured Publication Duerre, John A. (University of North Dakota, Grand Forks), and Patrick J. Buckley. Pigment production from tryptophan by an Achromobacter species. J. Bacteriol. 90:1686-1691. 1965.-A microorganism was isolated from the soil near the University of North Dak ... Full text Link to item Cite