Robert D. Pearlstein

Journal Article J Cereb Blood Flow Metab · June 2015 Hepatocyte growth factor (HGF), efficacious in preclinical models of acute central nervous system injury, is burdened by administration of full-length proteins. A multiinstitutional consortium investigated the efficacy of BB3, a small molecule with HGF-lik ... Full text Open Access Link to item Cite

Metalloporphyrins as therapeutic catalytic oxidoreductants in central nervous system disorders.

Journal Article Antioxid Redox Signal · May 20, 2014 SIGNIFICANCE: Metalloporphyrins, characterized by a redox-active transitional metal (Mn or Fe) coordinated to a cyclic porphyrin core ligand, mitigate oxidative/nitrosative stress in biological systems. Side-chain substitutions tune redox properties of met ... Full text Open Access Link to item Cite

Superoxide dismutase mimic, MnTE-2-PyP(5+) ameliorates acute and chronic proctitis following focal proton irradiation of the rat rectum.

Journal Article Redox Biol · 2013 Radiation proctitis, an inflammation and damage to the lower part of colon, is a common adverse event of the radiotherapy of tumors in the abdominal and pelvic region (colon, prostate, cervical). Several Mn(III) porphyrin-based superoxide dismutase mimics ... Full text Link to item Cite

Small ubiquitin-like modifier 1-3 conjugation [corrected] is activated in human astrocytic brain tumors and is required for glioblastoma cell survival.

Journal Article Cancer Sci · January 2013 Small ubiquitin-like modifier (SUMO1-3) constitutes a group of proteins that conjugate to lysine residues of target proteins thereby modifying their activity, stability, and subcellular localization. A large number of SUMO target proteins are transcription ... Full text Link to item Cite

Xenon neuroprotection in experimental stroke: interactions with hypothermia and intracerebral hemorrhage.

Journal Article Anesthesiology · December 2012 BACKGROUND: Xenon has been proven to be neuroprotective in experimental brain injury. The authors hypothesized that xenon would improve outcome from focal cerebral ischemia with a delayed treatment onset and prolonged recovery interval. METHODS: Rats were ... Full text Open Access Link to item Cite

Transient mild hypothermia differentially alters mitotic activity in normal and post-ischemic hippocampal slices from neonatal rats

Journal Article Journal of Thermal Biology · May 1, 2010 The purpose of this study was to determine if mild hypothermia alters mitotic activity in normal and post-ischemic hippocampal slices. (1) Normothermic oxygen-glucose deprivation (OGD 60 min) increased mitotic activity in the hippocampus up to 4d post-OGD. ... Full text Cite

A metalloporphyrin antioxidant alters cytokine responses after irradiation in a prostate tumor model.

Journal Article Radiat Res · April 2010 The goal of this study was to evaluate cytokine secretion capacity in a mouse model of prostate cancer, both with and without metalloporphyrin antioxidant and radiation treatment. C57BL/6 mice with subcutaneous RM-9 tumors were treated daily for 12 days wi ... Full text Link to item Cite

Metalloporphyrin antioxidants ameliorate normal tissue radiation damage in rat brain.

Journal Article Int J Radiat Biol · February 2010 PURPOSE: We examined the effects of manganese (III) meso-tetrakis (diethyl-2-5-imidazole) porphyrin, a metalloporphyrin antioxidant (MPA), on neural tissue radiation toxicity in vivo and on tumour cell radiosensitivity in vitro. MATERIALS AND METHODS: MPA ... Full text Link to item Cite

Long-term neuroprotection from a potent redox-modulating metalloporphyrin in the rat.

Journal Article Free Radic Biol Med · October 1, 2009 Sustained oxidative stress is a known sequel to focal cerebral ischemia. This study examined the effects of treatment with a single dose or sustained infusion of the redox-modulating MnPorphyrin Mn(III)TDE-2-ImP(5+) on outcome from middle cerebral artery o ... Full text Link to item Cite

Thermal vapor bubble and pressure dynamics during infrared laser ablation of tissue

Journal Article Applied Physics Letters · January 19, 2009 Free-electron laser irradiation can superheat tissue water, driving thermal vapor bubbles confined by tissue matrix and leading to mechanical tissue failure (ablation). Acoustic transients propagating from an ablation cavity were recorded with a polarizati ... Full text Cite

Effect of a metalloporphyrin antioxidant (MnTE-2-PyP) on the response of a mouse prostate cancer model to radiation.

Journal Article Anticancer Res · January 2009 BACKGROUND: Metalloporphyrin antioxidants can protect tissues against radiation-induced damage. However, for effective use in radiotherapy as normal tissue radioprotectants, they must not protect the cancer. The major objectives were to evaluate the effect ... Link to item Cite

Technical note: rapid prototyping of 3D grid arrays for image guided therapy quality assurance.

Journal Article Med Phys · December 2008 Three dimensional grid phantoms offer a number of advantages for measuring imaging related spatial inaccuracies for image guided surgery and radiotherapy. The authors examined the use of rapid prototyping technology for directly fabricating 3D grid phantom ... Full text Link to item Cite

Infrared imaging of trauma patients for detection of acute compartment syndrome of the leg.

Journal Article Crit Care Med · June 2008 OBJECTIVE: Early compartment syndrome is difficult to diagnose, and a delay in the diagnosis can result in amputation or death. Our objective was to explore the potential of infrared imaging, a portable and noninvasive technology, for detecting compartment ... Full text Link to item Cite

Pressure (mechanical) effects in infrared tissue ablation

Journal Article Progress in Biomedical Optics and Imaging Proceedings of SPIE · April 21, 2008 We experimentally demonstrate that the acoustic transients propagating as a result Free-Electron Laser (FEL) ablation in brain tissue exhibit a strong FEL wavelength dependence. These acoustic transients were measured with a time-resolved, polarization qua ... Full text Cite

Radiation and a metalloporphyrin radioprotectant in a mouse prostate tumor model

Journal Article Anticancer Research · September 1, 2007 Background: Antioxidants have the potential to protect normal tissues against radiation-induced damage, but must not protect tumor cells during radiotherapy. The major objectives were to determine whether a metalloporphyrin antioxidant affects prostate tum ... Cite

6450 nm wavelength tissue ablation using a nanosecond laser based on difference frequency mixing and stimulated Raman scattering.

Journal Article Opt Lett · June 1, 2007 A four-stage laser system was developed, emitting at a wavelength of 6450 nm with a 3-5 ns pulse duration, < or = 2 mJ pulse energy, and 1/2 Hz pulse repetition rate. The laser system successfully ablated rat brain tissue, where both the collateral damage ... Full text Link to item Cite

Isoflurane provides long-term protection against focal cerebral ischemia in the rat.

Journal Article Anesthesiology · January 2007 BACKGROUND: Long-term neuroprotection by isoflurane has been questioned. The authors examined factors in experimental models potentially critical to definition of enduring isoflurane neuroprotection. METHODS: Rats were prepared for temporary middle cerebra ... Full text Link to item Cite

NMDA-induced apoptosis in mixed neuronal/glial cortical cell cultures: the effects of isoflurane and dizocilpine.

Journal Article J Neurosurg Anesthesiol · October 2006 In animal models of severe ischemia, it has not been uniformly observed that anesthetics are protective. However, anesthetics have not been evaluated in the presence of a mild excitotoxic insult. We hypothesized that in the presence of a mild excitotoxic i ... Full text Link to item Cite

Selective gamma-aminobutyric acid type A receptor antagonism reverses isoflurane ischemic neuroprotection.

Journal Article Anesthesiology · July 2006 BACKGROUND: Isoflurane provides protection against severe forebrain ischemia in the rat. The authors hypothesized that this is attributable to interaction with the gamma-aminobutyric acid type A (GABAA) receptor resulting in altered time to onset of ischem ... Full text Link to item Cite

Assessing neurocognitive dysfunction in cranial radiotherapy: can cognitive event-related potentials help?

Journal Article Technol Cancer Res Treat · April 2006 Cognitive changes are common sequelae of cancer and cancer treatment, particularly in patients receiving cranial radiotherapy (RT). These effects are typically assessed by subjective clinical examination or using objective neuropsychological tests. Biologi ... Full text Link to item Cite

Effects of a manganese (III) porphyrin catalytic antioxidant in a mouse closed head injury model.

Journal Article Eur J Pharmacol · February 15, 2006 Closed head injury induces cerebral oxidative stress. The efficacy of a Mn (III) porphyrin catalytic antioxidant was assessed in a mouse closed head injury model. Mice were subjected to closed head injury and treated 15 min later with an i.v. bolus of vehi ... Full text Link to item Cite

Isoflurane provides sustained neuroprotection in rats subjected to focal ischemia

Journal Article Journal of Neurosurgical Anesthesiology · January 1, 2006 Abstracts published in the Journal of Neurosurgical Anesthesiology have been reviewed by the organizations or JNA Affiliate Societies at whose meetings the abstracts have been accepted for presentation. These abstracts have not undergone review by the Edit ... Full text Cite

Isoflurane-induced neuronal degeneration: an evaluation in organotypic hippocampal slice cultures.

Journal Article Anesth Analg · September 2005 Prolonged exposure of postnatal day (PND) 7 rat pups to anesthetics, which act via N-methyl-D-aspartate antagonism and/or gamma-amino butyric acid enhancement, causes neurodegeneration and persistent behavioral deficits. We studied these findings in vitro ... Full text Link to item Cite

Intrathecal administration of a novel apoE-derived therapeutic peptide improves outcome following perinatal hypoxic-ischemic injury.

Journal Article Neurosci Lett · June 24, 2005 Perinatal hypoxic-ischemic brain injury remains a significant clinical problem for which there remains no adequate therapeutic intervention. Apolipoprotein E (apoE) is a 299 amino acid protein that has been demonstrated to modify functional recovery follow ... Full text Link to item Cite

Apoptosis is not enhanced in primary mixed neuronal/glial cultures protected by isoflurane against N-methyl-D-aspartate excitotoxicity.

Journal Article Anesth Analg · December 2004 Volatile anesthetics reduce acute excitotoxic cell death in primary neuronal/glial cultures. We hypothesized that cells protected by isoflurane against N-methyl-d-aspartate (NMDA)-induced necrosis would instead become apoptotic. Primary mixed neuronal/glia ... Full text Link to item Cite

Hemodilution during cardiopulmonary bypass increases cerebral infarct volume after middle cerebral artery occlusion in rats.

Journal Article Anesth Analg · October 2004 Although the optimal hematocrit during cardiopulmonary bypass (CPB) is not defined, excessive hemodilution may lead to organ ischemia via a reduction in oxygen-carrying capacity uncompensated by autoregulatory and/or rheologic increases in organ blood flow ... Full text Link to item Cite

Intraischemic nitrous oxide alters neither neurologic nor histologic outcome: a comparison with dizocilpine.

Journal Article Anesth Analg · September 2004 N-Methyl-D-aspartate receptor antagonism contributes to the anesthetic action of nitrous oxide (N(2)O). We examined the effects of the N-methyl-D-aspartate antagonists N(2)O and dizocilpine on outcome from filament occlusion of the middle cerebral artery ( ... Full text Link to item Cite

Infrared thermography: a rapid, portable, and accurate technique to detect experimental pneumothorax.

Journal Article J Surg Res · August 2004 RATIONALE AND OBJECTIVE: Pneumothorax (Ptx) is a life-threatening complication that can result from trauma, mechanical ventilation, and invasive procedures. Infrared thermography (IRT), a compact and portable technology, has become highly sensitive. We hyp ... Full text Link to item Cite

Effects of isoflurane versus fentanyl-nitrous oxide anesthesia on long-term outcome from severe forebrain ischemia in the rat.

Journal Article Anesthesiology · May 2004 BACKGROUND: This study examined long-term outcome from severe forebrain ischemia in the rat, as a function of anesthetic given during the ischemic injury. METHODS: Rats were subjected to 10 min of near-complete forebrain ischemia while anesthetized with ei ... Full text Link to item Cite

A no-laminectomy spinal cord compression injury model in mice.

Journal Article J Neurotrauma · May 2004 The purpose of this study was to develop a minimally invasive recovery model of spinal cord injury in the C57Bl/6J mouse. Without laminectomy, the epidural space was exposed by disruption of the T10-T11 interspinous ligament. Perpendicular to the rostral-c ... Full text Link to item Cite

Apolipoprotein E protects against oxidative stress in mixed neuronal-glial cell cultures by reducing glutamate toxicity.

Journal Article Neurochem Int · January 2004 Apolipoprotein E (ApoE) deficiency has been shown to adversely affect outcome after transient cerebral ischemia and head trauma. Since oxidative stress contributes to these injuries, the ability of ApoE to reduce irreversible oxidative damage was studied i ... Full text Link to item Cite

Severe hypotension is not essential for isoflurane neuroprotection against forebrain ischemia in mice.

Journal Article Anesthesiology · November 2003 BACKGROUND: Volatile anesthetics provide protection in experimental models of global cerebral ischemia. To date, all models evaluated have included profound systemic arterial hypotension as a component of the ischemic insult. This study was designed to det ... Full text Link to item Cite

Brain ablation in the rat cerebral cortex using a tunable-free electron laser.

Journal Article Lasers Surg Med · 2003 BACKGROUND AND OBJECTIVES: We used the MARK III free electron laser (FEL) tuned to molecular vibrational absorbance maxima in the infrared (IR) wavelength range of 3.0-6.45 microm to study the effect of these various wavelengths and a power level of 5 mJ/2 ... Full text Link to item Cite

Protective effect of apolipoprotein E-mimetic peptides on N-methyl-D-aspartate excitotoxicity in primary rat neuronal-glial cell cultures.

Journal Article Neuroscience · 2003 Apolipoprotein E (apoE) is a 34-kD protein with multiple biological properties. Recent clinical and preclinical observations implicate a role for apoE in modifying the response of the brain to focal and global ischemia. One mechanism by which apoE might ex ... Full text Link to item Cite

Apolipoprotein E expression and behavioral toxicity of high charge, high energy (HZE) particle radiation.

Journal Article J Radiat Res · December 2002 UNLABELLED: Apolipoprotein E (apoE) is a lipid binding protein that plays an important role in tissue repair following brain injury. In the present studies, we have investigated whether apoE affects the behavioral toxicity of high charge, high energy (HZE) ... Full text Link to item Cite

Simvastatin increases endothelial nitric oxide synthase and ameliorates cerebral vasospasm resulting from subarachnoid hemorrhage.

Journal Article Stroke · December 2002 BACKGROUND AND PURPOSE: Endothelial nitric oxide synthase (eNOS) activity is decreased after subarachnoid hemorrhage (SAH). Simvastatin increases eNOS activity. We hypothesized that simvastatin would increase eNOS protein and ameliorate SAH-induced cerebra ... Full text Link to item Cite

A catalytic antioxidant (AEOL 10150) attenuates expression of inflammatory genes in stroke.

Journal Article Free Radic Biol Med · October 15, 2002 Oxidative stress is a major source of injury from cerebral ischemia and reperfusion. We hypothesized that a catalytic antioxidant AEOL 10150 [manganese (III) meso-tetrakis (di-N-ethylimidazole) porphyrin] would attenuate changes in brain gene expression in ... Full text Link to item Cite

Effects of metalloporphyrin catalytic antioxidants in experimental brain ischemia.

Journal Article Free Radic Biol Med · October 1, 2002 Reactive oxygen species play a role in the response of brain to ischemia. The effects of metalloporphyrin catalytic antioxidants (AEOL 10113 and AEOL 10150) were examined after murine middle cerebral artery occlusion (MCAO). Ninety minutes after reperfusio ... Full text Link to item Cite

Apolipoprotein E protects against NMDA excitotoxicity.

Journal Article Neurobiol Dis · October 2002 Preclinical and clinical evidence implicates a role for endogenous apolipoprotein E in modifying the response of the brain to focal and global ischemia. To investigate whether apoE modulates the neuronal response to glutamate excitotoxicity, we exposed pri ... Full text Link to item Cite

Attenuation of cerebral vasospasm after subarachnoid hemorrhage in mice overexpressing extracellular superoxide dismutase.

Journal Article Stroke · September 2002 BACKGROUND AND PURPOSE: Subarachnoid hemorrhage (SAH) increases production of vascular extracellular superoxide anion (*O2-). We examined whether overexpression of murine extracellular superoxide dismutase (EC-SOD) alters SAH-induced cerebral vasospasm, ox ... Full text Link to item Cite

Allopregnanolone attenuates N-methyl-D-aspartate-induced excitotoxicity and apoptosis in the human NT2 cell line in culture.

Journal Article Neurosci Lett · August 2, 2002 Progesterone modulates gamma-aminobutyric acid and excitatory amino acid neurotransmitter systems and has neuroprotective properties in models of hypoxia-ischemia. This study examined the in vitro effects of allopregnanolone, the active progesterone metabo ... Full text Link to item Cite

Mouse model of subarachnoid hemorrhage associated cerebral vasospasm: methodological analysis.

Journal Article Neurol Res · July 2002 The transgenic mouse has been used to study subarachnoid hemorrhage (SAH) induced delayed cerebral vasospasm (DCV). Methodological parameters have not been analyzed to validate this model and associated neurological deficits have not been described. We int ... Full text Link to item Cite

Pharmacological correction of hypothermic P(50) shift does not alter outcome from focal cerebral ischemia in rats.

Journal Article Am J Physiol Heart Circ Physiol · May 2002 Hypothermia decreases the arterial PO(2) at which hemoglobin is 50% saturated (P(50)), increasing hemoglobin O(2)-binding affinity. We used RSR13, a synthetic allosteric modifier of hemoglobin that increases P(50), to study the role of altered hemoglobin O ... Full text Link to item Cite

Exposure of the dorsal root ganglion in rats to pulsed radiofrequency currents activates dorsal horn lamina I and II neurons.

Journal Article Neurosurgery · April 2002 OBJECTIVE: Application of pulsed radiofrequency (RF) currents to the dorsal ganglion has been reported to produce long-term relief of spinal pain without causing thermal ablation. The present study was undertaken to identify spinal cord neurons activated b ... Full text Link to item Cite

Effects of volatile anesthetics on N-methyl-D-aspartate excitotoxicity in primary rat neuronal-glial cultures.

Journal Article Anesthesiology · September 2001 BACKGROUND: Volatile anesthetics are known to ameliorate experimental ischemic brain injury. A possible mechanism is inhibition of excitotoxic cascades induced by excessive glutamatergic stimulation. This study examined interactions between volatile anesth ... Full text Link to item Cite

Neuroprotection from delayed postischemic administration of a metalloporphyrin catalytic antioxidant.

Journal Article J Neurosci · July 1, 2001 Reactive oxygen species contribute to ischemic brain injury. This study examined whether the porphyrin catalytic antioxidant manganese (III) meso-tetrakis (N-ethylpyridinium-2-yl)porphyrin (MnTE-2-PyP(5+)) reduces oxidative stress and improves outcome from ... Full text Link to item Cite

Extracellular superoxide dismutase overexpression improves behavioral outcome from closed head injury in the mouse.

Journal Article J Neurotrauma · June 2001 Oxidative stress is known to play an important role in the response of brain to traumatic insults. We tested the hypothesis that increased extracellular superoxide dismutase (EC-SOD) expression can reduce injury in a mouse model of closed head injury. Neur ... Full text Link to item Cite

Absence of direct antioxidant effects from volatile anesthetics in primary mixed neuronal-glial cultures.

Journal Article Anesthesiology · February 2001 BACKGROUND: Volatile anesthetics decrease ischemic brain injury. Mechanisms for this protection remain under investigation. The authors hypothesized that volatile anesthetics serve as antioxidants in a neuronal-glial cell culture system. METHODS: Primary c ... Full text Link to item Cite

Periischemic cerebral blood flow (CBF) does not explain beneficial effects of isoflurane on outcome from near-complete forebrain ischemia in rats.

Journal Article Anesthesiology · October 2000 BACKGROUND: Isoflurane improves outcome from near-complete forebrain ischemia in rats compared with fentanyl-nitrous oxide (N2O). Sympathetic ganglionic blockade with trimethaphan abolishes this beneficial effect. To evaluate whether anesthesia-related dif ... Full text Link to item Cite

Anesthetic effects on cerebral metabolic rate predict histologic outcome from near-complete forebrain ischemia in the rat.

Journal Article Anesthesiology · August 2000 BACKGROUND: Although reduction of cerebral metabolic rate is thought to contribute to anesthetic neuroprotection, histologic evidence to support this concept has not been provided. In this study, histologic outcome was evaluated in rats subjected to differ ... Full text Link to item Cite

The effects of anesthetics on stress responses to forebrain ischemia and reperfusion in the rat.

Journal Article Anesth Analg · July 2000 UNLABELLED: Rats exposed to forebrain ischemia have reduced injury when anesthetized with isoflurane versus fentanyl + N(2)O. The protection caused by isoflurane is reversed by trimethaphan. We hypothesized that these anesthetic-dependent effects on ischem ... Full text Link to item Cite

A comparison of strain-related susceptibility in two murine recovery models of global cerebral ischemia.

Journal Article Brain Res · June 16, 2000 Genetically engineered mice are increasingly important in stroke research. The strains on which these constructs are built are known to have inherent differential sensitivities to ischemic insults. This has been largely attributed to differences in vascula ... Full text Link to item Cite

Mice overexpressing extracellular superoxide dismutase have increased resistance to global cerebral ischemia.

Journal Article Exp Neurol · June 2000 Transgenic mice, which exhibit a fivefold increase in brain parenchymal extracellular superoxide dismutase (EC-SOD) activity, were used to investigate the role of EC-SOD in global ischemic brain injury. Halothane-anesthetized normothermic wild-type (n = 22 ... Full text Link to item Cite

Effect of intracerebral norepinephrine depletion on outcome from severe forebrain ischemia in the rat.

Journal Article Brain Res · November 20, 1999 Manipulations of plasma catecholamine concentrations influence outcome from ischemic brain insults. It has been suggested that these effects are mediated by influences on brain catecholamine concentrations. This study examined whether major changes in brai ... Full text Link to item Cite

Pre-ischemic depletion of brain norepinephrine decreases infarct size in normothermic rats exposed to transient focal cerebral ischemia.

Journal Article Neurosci Lett · November 19, 1999 This study examined the importance of brain norepinephrine concentration on outcome from a focal ischemic insult. Fasted temperature-controlled male Wistar rats pretreated with DSP-4, (N-(chloroethyl)-N-ethyl-2-bromobenzylamine), to selectively deplete bra ... Full text Link to item Cite

Extracellular superoxide dismutase deficiency worsens outcome from focal cerebral ischemia in the mouse.

Journal Article Neurosci Lett · May 21, 1999 The role of endogenous extracellular superoxide dismutase (EC-SOD) was examined in a murine model of transient focal cerebral ischemia. Homozygous EC-SOD deficient (EC-SOD-/-; n = 18) and wild type (EC-SOD+/+; n = 19) littermates were anesthetized with hal ... Full text Link to item Cite

Regional metabolic changes in the pedunculopontine nucleus of unilateral 6-hydroxydopamine Parkinson's model rats.

Journal Article Brain Res · May 15, 1999 The pedunculopontine nucleus (PPN) located in the mesopontine tegmentum is innervated by descending projections from nuclei in the basal ganglia. The present study was performed to determine whether nigrostriatal dopaminergic neuron degeneration is associa ... Full text Link to item Cite

Effects of RSR13, a synthetic allosteric modifier of hemoglobin, alone and in combination with dizocilpine, on outcome from transient focal cerebral ischemia in the rat.

Journal Article Brain Res · May 1, 1999 This study examined the effect of a pharmacologically induced rightward shift in the partial pressure of oxygen at which 50% of hemoglobin is saturated (P50) on outcome from transient focal cerebral ischemia in the rat. Halothane anesthetized rats (n=20 pe ... Full text Link to item Cite

Apolipoprotein E deficiency worsens outcome from global cerebral ischemia in the mouse.

Journal Article Stroke · May 1999 BACKGROUND AND PURPOSE: Apolipoprotein E (apoE) has been found relevant in a variety of central nervous system disorders. This experiment examined the effect of endogenous murine apoE on selective neuronal necrosis resulting from a transient forebrain isch ... Full text Link to item Cite

Effects of isoflurane, ketamine, and fentanyl/N2O on concentrations of brain and plasma catecholamines during near-complete cerebral ischemia in the rat.

Journal Article Anesth Analg · April 1999 UNLABELLED: We postulated that adrenergic responses to global cerebral ischemia are anesthetic-dependent and similar in both brain and arterial blood. Rats were anesthetized with isoflurane (1.4%), ketamine (1 mg x kg(-1) x min(-1)), or fentanyl (25 microg ... Full text Link to item Cite

Characterization of a recovery global cerebral ischemia model in the mouse.

Journal Article J Neurosci Methods · April 1, 1999 Transgenic/knockout murine variants allow roles of specific proteins to be studied in cerebral ischemia. Because of the size of mice, however, study of prolonged recovery from global ischemia has been limited. This project characterized an adaptation of th ... Full text Link to item Cite

Sympathetic ganglionic blockade masks beneficial effect of isoflurane on histologic outcome from near-complete forebrain ischemia in the rat.

Journal Article Anesthesiology · March 1999 BACKGROUND: Isoflurane-anesthetized rats have better outcome from global cerebral ischemia than rats anesthetized with fentanyl and nitrous oxide. The authors wanted to determine whether circulating catecholamine concentrations depend on the anesthetic age ... Full text Link to item Cite

Effects of postischemic halothane administration on outcome from transient focal cerebral ischemia in the rat.

Journal Article J Neurosurg Anesthesiol · January 1999 This study examined the effect of prolonged postischemic halothane administration on outcome from transient focal cerebral ischemia in rats. Conscious normothermic rats were subjected to 75 minutes of filament middle cerebral artery occlusion (MCAO). Anima ... Full text Link to item Cite

Mice overexpressing extracellular superoxide dismutase have increased resistance to focal cerebral ischemia.

Journal Article Neuroscience · January 1999 Transgenic mice, which had been transfected with the human extracellular superoxide dismutase gene, causing an approximate five-fold increase in brain parenchymal extracellular superoxide dismutase activity, were used to investigate the role of extracellul ... Full text Link to item Cite

The effects of aprotinin on outcome from cerebral ischemia in the rat.

Journal Article Anesth Analg · January 1999 UNLABELLED: The administration of aprotinin has been associated with a reduction in cardiac surgery-related stroke. Intrinsic neuroprotective properties of this drug have not been evaluated in laboratory outcome models of cerebral ischemia. The purpose of ... Full text Link to item Cite

Critical care applications of infrared imaging

Journal Article Critical Care Medicine · January 1, 1999 Introduction: Vascular access is a fundamental component of caring for critically ill children and adults. Although a straightforward procedure, establishing vascular access can be very difficult and time consuming, especially in the sickest patients, who ... Full text Cite

Regional CBF in apolipoprotein E-deficient and wild type mice during focal cerebral ischemia.

Journal Article Neuroreport · August 3, 1998 Apolipoprotein E-(apoE) deficient mice exhibit hypercholesterolemia, accelerated atherosclerosis and increased infarct size after middle cerebral artery occlusion (MCAO). This study examined whether worsened ischemic outcome is attributable to effects of a ... Full text Link to item Cite

Effects of a synthetic allosteric modifier of hemoglobin oxygen affinity on outcome from global cerebral ischemia in the rat.

Journal Article Stroke · August 1998 BACKGROUND AND PURPOSE: Neuronal injury results from an insufficient supply of oxygen to the brain. This experiment examined whether a pharmacologically induced rightward shift of the partial pressure of oxygen at which 50% of hemoglobin is saturated (P50) ... Full text Link to item Cite

Differential effects of anesthetic agents on outcome from near-complete but not incomplete global ischemia in the rat.

Journal Article Anesthesiology · August 1998 BACKGROUND: It has been postulated that anesthetic agents that reduce cerebral metabolic rate will protect the brain against ischemia when electroencephalographic (EEG) activity is persistent, but will provide no protection when ischemia is severe enough t ... Full text Link to item Cite

Relative neuroprotective effects of dizocilpine and isoflurane during focal cerebral ischemia in the rat.

Journal Article Anesth Analg · July 1998 UNLABELLED: Both dizocilpine (MK-801) and isoflurane antagonize glutamatergic neurotransmission. In this study, we examined the relative neuroprotective effects of these drugs administered in equianesthetic doses before the onset of focal cerebral ischemia ... Full text Link to item Cite

Interactions between hypothermia and the latency to ischemic depolarization: implications for neuroprotection.

Journal Article Anesthesiology · May 1998 BACKGROUND: The authors postulated that hypothermic neuroprotection can be attributed to a delayed onset of ischemic depolarization. METHODS: Halothane-anesthetized rats were prepared for near-complete forebrain ischemia. Direct current (DC) potential micr ... Full text Link to item Cite

Neuroprotective effects of NMDA receptor glycine recognition site antagonism: dependence on glycine concentration.

Journal Article J Neurochem · May 1998 High-affinity NMDA receptor glycine recognition site antagonists protect brain tissue from ischemic damage. The neuroprotective effect of 5-nitro-6,7-dichloro-2,3-quinoxalinedione (ACEA 1021), a selective NMDA receptor antagonist with nanomolar affinity fo ... Full text Link to item Cite

Apolipoprotein E isoform-specific differences in outcome from focal ischemia in transgenic mice.

Journal Article J Cereb Blood Flow Metab · April 1998 Apolipoprotein E (apoE), a 34-KD glycosylated lipid-binding protein, is expressed as three common isoforms in humans (E2, E3, or E4). Clinical evidence suggests that the apoE genotype (APOE) may be a risk factor for poor outcome after acute central nervous ... Full text Link to item Cite

Effect of halothane in cortical cell cultures exposed to N-methyl-D-aspartate.

Journal Article Neurochem Res · January 1998 In vivo studies have shown potent protection by volatile anesthetic agents against cerebral ischemic insults. Volatile agents have also been shown to antagonize glutamatergic neurotransmission at the N-methyl-D-aspartate (NMDA) receptor. This study examine ... Full text Link to item Cite

Effects of NMDA receptor glycine recognition site antagonism on cerebral metabolic rate for glucose and cerebral blood flow in the conscious rat.

Journal Article Brain Res · January 1, 1998 Glycine is a requisite cofactor for glutamatergic activation of the N-methyl-D-aspartate (NMDA) receptor. Antagonism of glutamate at the NMDA receptor has been shown to cause substantial changes in regional cerebral metabolic rate for glucose utilization ( ... Full text Link to item Cite

Modulation of cerebral blood flow is not the mechanism of neuroprotection in transgenic mice over expressing human extracellular superoxide dismutase (EC-SOD)

Journal Article Critical Care Medicine · January 1, 1998 Introduction: Transgenic mice over expressing human EC-SOD (5-fold increase in brain) that have received a 90 min filament occlusion of the middle cerebral artery (MCAO) have a 27% decrease in infarct volume compared to wild type controls (1). Superoxide ( ... Full text Cite

Comparison of the effects of propofol and pentobarbital on neurologic outcome and cerebral infarct size after temporary focal ischemia in the rat.

Journal Article Anesthesiology · November 1997 BACKGROUND: Although propofol is known to have effects on cerebral physiology similar to the barbiturates, a direct comparison of the relative effects of these drugs on outcome from cerebral ischemia has not been performed. The authors postulated that pent ... Full text Link to item Cite

High-dose fentanyl does not adversely affect outcome from forebrain ischemia in the rat.

Journal Article J Neurosurg Anesthesiol · October 1997 Fentanyl citrate has properties, including agonism of the mu-opioid receptor and proconvulsant activity, that theoretically could pose adverse effects in ischemic brain. This study examined the effects of high-dose fentanyl on outcome in rats subjected to ... Full text Link to item Cite

Glycine antagonism does not block ischemic spontaneous depolarization in the rat.

Journal Article Neuroreport · March 24, 1997 This study examined the effect of glycine recognition site antagonism (ACEA 1021) on the incidence of spontaneous depolarizations in the penumbra of a focal ischemic lesion. Rats were administered either vehicle (n = 7), ACEA 1021 (n = 7) or dizocilpine (n ... Full text Link to item Cite

Neuroprotective effect of NMDA receptor glycine recognition site antagonism persists when brain temperature is controlled.

Journal Article J Cereb Blood Flow Metab · February 1997 Several lines of inquiry have indicated that glycine plays an important role in both glutamatergic neurotransmission and pathophysiology of cerebral ischemia. However, subacute outcome trials demonstrating the efficacy of glycine antagonists as neuroprotec ... Full text Link to item Cite

Concentrations of indoleamine metabolic intermediates in the ventricular cerebrospinal fluid of advanced Parkinson's patients with severe postural instability and gait disorders.

Journal Article J Neural Transm (Vienna) · 1997 Postural instability and gait disorders (PIGD) are the primary causes of disability in many but not all advanced Parkinson's disease (PD) patients. We have measured the concentrations of serotonin, 5-hydroxytryptophan (5-HTP), 5-hydroxy-3-indoleacetic acid ... Full text Link to item Cite

Hypothermia reduces the propensity of cortical tissue to propagate direct current depolarizations in the rat.

Journal Article Neurosci Lett · October 25, 1996 Both spreading depression (SD) and spontaneous cortical ischemic depolarizations are known to be sensitive to brain temperature. What is unknown is whether this temperature effect is caused by altered sensitivity of cortical tissue to the initiating stimul ... Full text Link to item Cite

Acute changes in intracranial pressure and pressure-volume index after forebrain ischemia in normoglycemic and hyperglycemic rats.

Journal Article Stroke · August 1996 BACKGROUND AND PURPOSE: Hyperglycemia enhances the deleterious effect of global cerebral ischemia. One possible explanation is that increased anaerobic glycolysis leads to exaggeration of intracellular acidosis and increased postischemic edema. To examine ... Full text Link to item Cite

Electroencephalographic burst suppression is not required to elicit maximal neuroprotection from pentobarbital in a rat model of focal cerebral ischemia.

Journal Article Anesthesiology · June 1996 BACKGROUND: Barbiturates have previously been demonstrated to reduce focal cerebral ischemic brain damage. However, the dose of drug required to elicit maximal neuroprotection has not been defined. The authors' hypothesized that doses of pentobarbital subs ... Full text Link to item Cite

Halothane reduces focal ischemic injury in the rat when brain temperature is controlled.

Journal Article Anesthesiology · May 1995 BACKGROUND: Previous work has demonstrated that rats anesthetized with halothane during focal cerebral ischemia have better histologic and neurologic outcome than do rats undergoing the same insult when awake. The purpose of this experiment was to determin ... Full text Link to item Cite

Halothane reduces focal ischemic injury in the rat when brain temperature is controlled

Journal Article Anesthesiology · January 1, 1995 Cite

Reversible uncoupling of oxidative phosphorylation at low oxygen tension.

Journal Article Proc Natl Acad Sci U S A · October 1983 The stoichiometry of oxidative phosphorylation at low oxygen tension (less than 3 torr; O2 less than 5 microM) has been measured in rat liver mitochondria. In a steady-state model in which respiration rate was experimentally controlled by either oxygen or ... Full text Link to item Cite

Neurogenic control of cerebral blood flow in the pathoetiology of cerebral vasospasm

Journal Article Surgical Forum · January 1, 1983 Cite

Effects of hyperbaric oxygen therapy on long-tract neuronal conduction in the acute phase of spinal cord injury.

Journal Article J Neurosurg · October 1981 To study the acute effects of hyperbaric oxygen ventilation (HBO) on long-tract function following spinal cord trauma, the authors employed a technique for monitoring spinal cord evoked potentials (SCEP) as an objective measure of translesion neuronal cond ... Full text Link to item Cite

Cerebral cortical microfluorometry at isosbestic wavelengths for correction of vascular artifact.

Journal Article Science · August 17, 1979 Microfluorometric measurements of cerebral cortical mitochondrial respiration in vivo are obscured by hemodynamic and oximetric artifacts. Isosbestic fluorometry provides appropriate correction for these vascular phenomena and permits simultaneous evaluati ... Full text Link to item Cite