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Assessing cardiac injury in mice with dual energy-microCT, 4D-microCT, and microSPECT imaging after partial heart irradiation.

Publication ,  Journal Article
Lee, C-L; Min, H; Befera, N; Clark, D; Qi, Y; Das, S; Johnson, GA; Badea, CT; Kirsch, DG
Published in: Int J Radiat Oncol Biol Phys
March 1, 2014

PURPOSE: To develop a mouse model of cardiac injury after partial heart irradiation (PHI) and to test whether dual energy (DE)-microCT and 4-dimensional (4D)-microCT can be used to assess cardiac injury after PHI to complement myocardial perfusion imaging using micro-single photon emission computed tomography (SPECT). METHODS AND MATERIALS: To study cardiac injury from tangent field irradiation in mice, we used a small-field biological irradiator to deliver a single dose of 12 Gy x-rays to approximately one-third of the left ventricle (LV) of Tie2Cre; p53(FL/+) and Tie2Cre; p53(FL/-) mice, where 1 or both alleles of p53 are deleted in endothelial cells. Four and 8 weeks after irradiation, mice were injected with gold and iodinated nanoparticle-based contrast agents, and imaged with DE-microCT and 4D-microCT to evaluate myocardial vascular permeability and cardiac function, respectively. Additionally, the same mice were imaged with microSPECT to assess myocardial perfusion. RESULTS: After PHI with tangent fields, DE-microCT scans showed a time-dependent increase in accumulation of gold nanoparticles (AuNp) in the myocardium of Tie2Cre; p53(FL/-) mice. In Tie2Cre; p53(FL/-) mice, extravasation of AuNp was observed within the irradiated LV, whereas in the myocardium of Tie2Cre; p53(FL/+) mice, AuNp were restricted to blood vessels. In addition, data from DE-microCT and microSPECT showed a linear correlation (R(2) = 0.97) between the fraction of the LV that accumulated AuNp and the fraction of LV with a perfusion defect. Furthermore, 4D-microCT scans demonstrated that PHI caused a markedly decreased ejection fraction, and higher end-diastolic and end-systolic volumes, to develop in Tie2Cre; p53(FL/-) mice, which were associated with compensatory cardiac hypertrophy of the heart that was not irradiated. CONCLUSIONS: Our results show that DE-microCT and 4D-microCT with nanoparticle-based contrast agents are novel imaging approaches complementary to microSPECT for noninvasive assessment of the change in myocardial vascular permeability and cardiac function of mice in whom myocardial injury develops after PHI.

Duke Scholars

Published In

Int J Radiat Oncol Biol Phys

DOI

EISSN

1879-355X

Publication Date

March 1, 2014

Volume

88

Issue

3

Start / End Page

686 / 693

Location

United States

Related Subject Headings

  • X-Ray Microtomography
  • Tomography, Emission-Computed, Single-Photon
  • Radiation Injuries, Experimental
  • Organotechnetium Compounds
  • Organophosphorus Compounds
  • Oncology & Carcinogenesis
  • Nanoparticles
  • Mice
  • Heart Ventricles
  • Heart
 

Citation

APA
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ICMJE
MLA
NLM
Lee, C.-L., Min, H., Befera, N., Clark, D., Qi, Y., Das, S., … Kirsch, D. G. (2014). Assessing cardiac injury in mice with dual energy-microCT, 4D-microCT, and microSPECT imaging after partial heart irradiation. Int J Radiat Oncol Biol Phys, 88(3), 686–693. https://doi.org/10.1016/j.ijrobp.2013.11.238
Lee, Chang-Lung, Hooney Min, Nicholas Befera, Darin Clark, Yi Qi, Shiva Das, G Allan Johnson, Cristian T. Badea, and David G. Kirsch. “Assessing cardiac injury in mice with dual energy-microCT, 4D-microCT, and microSPECT imaging after partial heart irradiation.Int J Radiat Oncol Biol Phys 88, no. 3 (March 1, 2014): 686–93. https://doi.org/10.1016/j.ijrobp.2013.11.238.
Lee C-L, Min H, Befera N, Clark D, Qi Y, Das S, et al. Assessing cardiac injury in mice with dual energy-microCT, 4D-microCT, and microSPECT imaging after partial heart irradiation. Int J Radiat Oncol Biol Phys. 2014 Mar 1;88(3):686–93.
Lee, Chang-Lung, et al. “Assessing cardiac injury in mice with dual energy-microCT, 4D-microCT, and microSPECT imaging after partial heart irradiation.Int J Radiat Oncol Biol Phys, vol. 88, no. 3, Mar. 2014, pp. 686–93. Pubmed, doi:10.1016/j.ijrobp.2013.11.238.
Lee C-L, Min H, Befera N, Clark D, Qi Y, Das S, Johnson GA, Badea CT, Kirsch DG. Assessing cardiac injury in mice with dual energy-microCT, 4D-microCT, and microSPECT imaging after partial heart irradiation. Int J Radiat Oncol Biol Phys. 2014 Mar 1;88(3):686–693.
Journal cover image

Published In

Int J Radiat Oncol Biol Phys

DOI

EISSN

1879-355X

Publication Date

March 1, 2014

Volume

88

Issue

3

Start / End Page

686 / 693

Location

United States

Related Subject Headings

  • X-Ray Microtomography
  • Tomography, Emission-Computed, Single-Photon
  • Radiation Injuries, Experimental
  • Organotechnetium Compounds
  • Organophosphorus Compounds
  • Oncology & Carcinogenesis
  • Nanoparticles
  • Mice
  • Heart Ventricles
  • Heart