Skip to main content

Restricted isotype, distinct variable gene usage, and high rate of gp120 specificity of HIV-1 envelope-specific B cells in colostrum compared with those in blood of HIV-1-infected, lactating African women.

Publication ,  Journal Article
Sacha, CR; Vandergrift, N; Jeffries, TL; McGuire, E; Fouda, GG; Liebl, B; Marshall, DJ; Gurley, TC; Stiegel, L; Whitesides, JF; Friedman, J ...
Published in: Mucosal Immunol
March 2015

A successful HIV-1 vaccine must elicit immune responses that impede mucosal virus transmission, though functional roles of protective HIV-1 Envelope (Env)-specific mucosal antibodies remain unclear. Colostrum is a rich source of readily accessible mucosal B cells that may help define the mucosal antibody response contributing to prevention of postnatal HIV-1 transmission. To examine the HIV-1 Env-specific colostrum B-cell repertoire, single B cells were isolated from 17 chronically HIV-infected, lactating women, producing 51 blood and 39 colostrum HIV-1 Env-specific B-cell antibodies. All HIV-1 Env-specific colostrum-derived antibodies were immunoglobulin (Ig)G1 isotype and had mean heavy chain complementarity-determining region 3 (CDR3) lengths and mutation frequencies similar to those isolated from blood. However, variable heavy chain (VH) gene subfamily 1(∼)69 usage was higher among colostrum than blood HIV-1 Env-reactive antibodies (49% vs. 20%, P=0.006, Fisher's exact test). Additionally, more HIV-1 Env-specific colostrum antibodies were gp120 specific than those isolated from blood (44% vs. 16%, P=0.005, Fisher's exact test). One cross-compartment HIV-1 Env-specific clonal B-cell lineage was identified. These unique characteristics of colostrum B-cell antibodies suggest selective homing of HIV-1-specific IgG1-secreting memory B cells to the mammary gland and have implications for targeting mucosal B-cell populations by vaccination.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Mucosal Immunol

DOI

EISSN

1935-3456

Publication Date

March 2015

Volume

8

Issue

2

Start / End Page

316 / 326

Location

United States

Related Subject Headings

  • Viral Load
  • Somatic Hypermutation, Immunoglobulin
  • Phenotype
  • Mutation Rate
  • Lactation
  • Infectious Disease Transmission, Vertical
  • Immunophenotyping
  • Immunology
  • Immunologic Memory
  • Immunoglobulin Variable Region
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Sacha, C. R., Vandergrift, N., Jeffries, T. L., McGuire, E., Fouda, G. G., Liebl, B., … Permar, S. R. (2015). Restricted isotype, distinct variable gene usage, and high rate of gp120 specificity of HIV-1 envelope-specific B cells in colostrum compared with those in blood of HIV-1-infected, lactating African women. Mucosal Immunol, 8(2), 316–326. https://doi.org/10.1038/mi.2014.69
Sacha, C. R., N. Vandergrift, T. L. Jeffries, E. McGuire, G. G. Fouda, B. Liebl, D. J. Marshall, et al. “Restricted isotype, distinct variable gene usage, and high rate of gp120 specificity of HIV-1 envelope-specific B cells in colostrum compared with those in blood of HIV-1-infected, lactating African women.Mucosal Immunol 8, no. 2 (March 2015): 316–26. https://doi.org/10.1038/mi.2014.69.
Sacha CR, Vandergrift N, Jeffries TL, McGuire E, Fouda GG, Liebl B, Marshall DJ, Gurley TC, Stiegel L, Whitesides JF, Friedman J, Badiabo A, Foulger A, Yates NL, Tomaras GD, Kepler TB, Liao HX, Haynes BF, Moody MA, Permar SR. Restricted isotype, distinct variable gene usage, and high rate of gp120 specificity of HIV-1 envelope-specific B cells in colostrum compared with those in blood of HIV-1-infected, lactating African women. Mucosal Immunol. 2015 Mar;8(2):316–326.

Published In

Mucosal Immunol

DOI

EISSN

1935-3456

Publication Date

March 2015

Volume

8

Issue

2

Start / End Page

316 / 326

Location

United States

Related Subject Headings

  • Viral Load
  • Somatic Hypermutation, Immunoglobulin
  • Phenotype
  • Mutation Rate
  • Lactation
  • Infectious Disease Transmission, Vertical
  • Immunophenotyping
  • Immunology
  • Immunologic Memory
  • Immunoglobulin Variable Region