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Antibody to the gp120 V1/V2 loops and CD4+ and CD8+ T cell responses in protection from SIVmac251 vaginal acquisition and persistent viremia.

Publication ,  Journal Article
Gordon, SN; Doster, MN; Kines, RC; Keele, BF; Brocca-Cofano, E; Guan, Y; Pegu, P; Liyanage, NPM; Vaccari, M; Cuburu, N; Buck, CB; Ferrari, G ...
Published in: J Immunol
December 15, 2014

The human papillomavirus pseudovirions (HPV-PsVs) approach is an effective gene-delivery system that can prime or boost an immune response in the vaginal tract of nonhuman primates and mice. Intravaginal vaccination with HPV-PsVs expressing SIV genes, combined with an i.m. gp120 protein injection, induced humoral and cellular SIV-specific responses in macaques. Priming systemic immune responses with i.m. immunization with ALVAC-SIV vaccines, followed by intravaginal HPV-PsV-SIV/gp120 boosting, expanded and/or recruited T cells in the female genital tract. Using a stringent repeated low-dose intravaginal challenge with the highly pathogenic SIVmac251, we show that although these regimens did not demonstrate significant protection from virus acquisition, they provided control of viremia in a number of animals. High-avidity Ab responses to the envelope gp120 V1/V2 region correlated with delayed SIVmac251 acquisition, whereas virus levels in mucosal tissues were inversely correlated with antienvelope CD4(+) T cell responses. CD8(+) T cell depletion in animals with controlled viremia caused an increase in tissue virus load in some animals, suggesting a role for CD8(+) T cells in virus control. This study highlights the importance of CD8(+) cells and antienvelope CD4(+) T cells in curtailing virus replication and antienvelope V1/V2 Abs in preventing SIVmac251 acquisition.

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Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

December 15, 2014

Volume

193

Issue

12

Start / End Page

6172 / 6183

Location

United States

Related Subject Headings

  • Viremia
  • Viral Envelope Proteins
  • Vagina
  • Vaccines, DNA
  • Vaccination
  • T-Lymphocyte Subsets
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Simian Acquired Immunodeficiency Syndrome
  • SAIDS Vaccines
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Gordon, S. N., Doster, M. N., Kines, R. C., Keele, B. F., Brocca-Cofano, E., Guan, Y., … Franchini, G. (2014). Antibody to the gp120 V1/V2 loops and CD4+ and CD8+ T cell responses in protection from SIVmac251 vaginal acquisition and persistent viremia. J Immunol, 193(12), 6172–6183. https://doi.org/10.4049/jimmunol.1401504
Gordon, Shari N., Melvin N. Doster, Rhonda C. Kines, Brandon F. Keele, Egidio Brocca-Cofano, Yongjun Guan, Poonam Pegu, et al. “Antibody to the gp120 V1/V2 loops and CD4+ and CD8+ T cell responses in protection from SIVmac251 vaginal acquisition and persistent viremia.J Immunol 193, no. 12 (December 15, 2014): 6172–83. https://doi.org/10.4049/jimmunol.1401504.
Gordon SN, Doster MN, Kines RC, Keele BF, Brocca-Cofano E, Guan Y, et al. Antibody to the gp120 V1/V2 loops and CD4+ and CD8+ T cell responses in protection from SIVmac251 vaginal acquisition and persistent viremia. J Immunol. 2014 Dec 15;193(12):6172–83.
Gordon, Shari N., et al. “Antibody to the gp120 V1/V2 loops and CD4+ and CD8+ T cell responses in protection from SIVmac251 vaginal acquisition and persistent viremia.J Immunol, vol. 193, no. 12, Dec. 2014, pp. 6172–83. Pubmed, doi:10.4049/jimmunol.1401504.
Gordon SN, Doster MN, Kines RC, Keele BF, Brocca-Cofano E, Guan Y, Pegu P, Liyanage NPM, Vaccari M, Cuburu N, Buck CB, Ferrari G, Montefiori D, Piatak M, Lifson JD, Xenophontos AM, Venzon D, Robert-Guroff M, Graham BS, Lowy DR, Schiller JT, Franchini G. Antibody to the gp120 V1/V2 loops and CD4+ and CD8+ T cell responses in protection from SIVmac251 vaginal acquisition and persistent viremia. J Immunol. 2014 Dec 15;193(12):6172–6183.

Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

December 15, 2014

Volume

193

Issue

12

Start / End Page

6172 / 6183

Location

United States

Related Subject Headings

  • Viremia
  • Viral Envelope Proteins
  • Vagina
  • Vaccines, DNA
  • Vaccination
  • T-Lymphocyte Subsets
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Simian Acquired Immunodeficiency Syndrome
  • SAIDS Vaccines