Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel

Regulation of chromatin accessibility and Zic binding at enhancers in the developing cerebellum.

Publication ,  Journal Article
Frank, CL; Liu, F; Wijayatunge, R; Song, L; Biegler, MT; Yang, MG; Vockley, CM; Safi, A; Gersbach, CA; Crawford, GE; West, AE
Published in: Nat Neurosci
May 2015

To identify chromatin mechanisms of neuronal differentiation, we characterized chromatin accessibility and gene expression in cerebellar granule neurons (CGNs) of the developing mouse. We used DNase-seq to map accessibility of cis-regulatory elements and RNA-seq to profile transcript abundance across postnatal stages of neuronal differentiation in vivo and in culture. We observed thousands of chromatin accessibility changes as CGNs differentiated, and verified, using H3K27ac ChIP-seq, reporter gene assays and CRISPR-mediated activation, that many of these regions function as neuronal enhancers. Motif discovery in differentially accessible chromatin regions suggested a previously unknown role for the Zic family of transcription factors in CGN maturation. We confirmed the association of Zic with these elements by ChIP-seq and found, using knockdown, that Zic1 and Zic2 are required for coordinating mature neuronal gene expression patterns. Together, our data reveal chromatin dynamics at thousands of gene regulatory elements that facilitate the gene expression patterns necessary for neuronal differentiation and function.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Nat Neurosci

DOI

EISSN

1546-1726

Publication Date

May 2015

Volume

18

Issue

5

Start / End Page

647 / 656

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Neurons
  • Neurology & Neurosurgery
  • Neurogenesis
  • Nerve Tissue Proteins
  • Molecular Sequence Data
  • Mice
  • Histones
  • Genes, Reporter
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Frank, C. L., Liu, F., Wijayatunge, R., Song, L., Biegler, M. T., Yang, M. G., … West, A. E. (2015). Regulation of chromatin accessibility and Zic binding at enhancers in the developing cerebellum. Nat Neurosci, 18(5), 647–656. https://doi.org/10.1038/nn.3995
Frank, Christopher L., Fang Liu, Ranjula Wijayatunge, Lingyun Song, Matthew T. Biegler, Marty G. Yang, Christopher M. Vockley, et al. “Regulation of chromatin accessibility and Zic binding at enhancers in the developing cerebellum.Nat Neurosci 18, no. 5 (May 2015): 647–56. https://doi.org/10.1038/nn.3995.
Frank CL, Liu F, Wijayatunge R, Song L, Biegler MT, Yang MG, et al. Regulation of chromatin accessibility and Zic binding at enhancers in the developing cerebellum. Nat Neurosci. 2015 May;18(5):647–56.
Frank, Christopher L., et al. “Regulation of chromatin accessibility and Zic binding at enhancers in the developing cerebellum.Nat Neurosci, vol. 18, no. 5, May 2015, pp. 647–56. Pubmed, doi:10.1038/nn.3995.
Frank CL, Liu F, Wijayatunge R, Song L, Biegler MT, Yang MG, Vockley CM, Safi A, Gersbach CA, Crawford GE, West AE. Regulation of chromatin accessibility and Zic binding at enhancers in the developing cerebellum. Nat Neurosci. 2015 May;18(5):647–656.

Published In

Nat Neurosci

DOI

EISSN

1546-1726

Publication Date

May 2015

Volume

18

Issue

5

Start / End Page

647 / 656

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Neurons
  • Neurology & Neurosurgery
  • Neurogenesis
  • Nerve Tissue Proteins
  • Molecular Sequence Data
  • Mice
  • Histones
  • Genes, Reporter