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2μ plasmid in Saccharomyces species and in Saccharomyces cerevisiae.

Publication ,  Journal Article
Strope, PK; Kozmin, SG; Skelly, DA; Magwene, PM; Dietrich, FS; McCusker, JH
Published in: FEMS Yeast Res
December 2015

We determined that extrachromosomal 2μ plasmid was present in 67 of the Saccharomyces cerevisiae 100-genome strains; in addition to variation in the size and copy number of 2μ, we identified three distinct classes of 2μ. We identified 2μ presence/absence and class associations with populations, clinical origin and nuclear genotypes. We also screened genome sequences of S. paradoxus, S. kudriavzevii, S. uvarum, S. eubayanus, S. mikatae, S. arboricolus and S. bayanus strains for both integrated and extrachromosomal 2μ. Similar to S. cerevisiae, we found no integrated 2μ sequences in any S. paradoxus strains. However, we identified part of 2μ integrated into the genomes of some S. uvarum, S. kudriavzevii, S. mikatae and S. bayanus strains, which were distinct from each other and from all extrachromosomal 2μ. We identified extrachromosomal 2μ in one S. paradoxus, one S. eubayanus, two S. bayanus and 13 S. uvarum strains. The extrachromosomal 2μ in S. paradoxus, S. eubayanus and S. cerevisiae were distinct from each other. In contrast, the extrachromosomal 2μ in S. bayanus and S. uvarum strains were identical with each other and with one of the three classes of S. cerevisiae 2μ, consistent with interspecific transfer.

Duke Scholars

Published In

FEMS Yeast Res

DOI

EISSN

1567-1364

Publication Date

December 2015

Volume

15

Issue

8

Location

England

Related Subject Headings

  • Saccharomyces
  • Plasmids
  • Microbiology
  • Interspersed Repetitive Sequences
  • Genetic Variation
  • 40 Engineering
  • 31 Biological sciences
  • 10 Technology
  • 09 Engineering
  • 06 Biological Sciences
 

Citation

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Strope, P. K., Kozmin, S. G., Skelly, D. A., Magwene, P. M., Dietrich, F. S., & McCusker, J. H. (2015). 2μ plasmid in Saccharomyces species and in Saccharomyces cerevisiae. FEMS Yeast Res, 15(8). https://doi.org/10.1093/femsyr/fov090
Strope, Pooja K., Stanislav G. Kozmin, Daniel A. Skelly, Paul M. Magwene, Fred S. Dietrich, and John H. McCusker. “2μ plasmid in Saccharomyces species and in Saccharomyces cerevisiae.FEMS Yeast Res 15, no. 8 (December 2015). https://doi.org/10.1093/femsyr/fov090.
Strope PK, Kozmin SG, Skelly DA, Magwene PM, Dietrich FS, McCusker JH. 2μ plasmid in Saccharomyces species and in Saccharomyces cerevisiae. FEMS Yeast Res. 2015 Dec;15(8).
Strope, Pooja K., et al. “2μ plasmid in Saccharomyces species and in Saccharomyces cerevisiae.FEMS Yeast Res, vol. 15, no. 8, Dec. 2015. Pubmed, doi:10.1093/femsyr/fov090.
Strope PK, Kozmin SG, Skelly DA, Magwene PM, Dietrich FS, McCusker JH. 2μ plasmid in Saccharomyces species and in Saccharomyces cerevisiae. FEMS Yeast Res. 2015 Dec;15(8).
Journal cover image

Published In

FEMS Yeast Res

DOI

EISSN

1567-1364

Publication Date

December 2015

Volume

15

Issue

8

Location

England

Related Subject Headings

  • Saccharomyces
  • Plasmids
  • Microbiology
  • Interspersed Repetitive Sequences
  • Genetic Variation
  • 40 Engineering
  • 31 Biological sciences
  • 10 Technology
  • 09 Engineering
  • 06 Biological Sciences