Impact of synchronized anti-PD-1 with Ad-CEA vaccination on inhibition of colon cancer growth.
Aim: The purpose of this study was to determine whether addition of anti-PD-1 antibody increased the immunogenicity and anti-tumor activity of Ad-CEA vaccination in a murine model of colon cancer. Methods: Ad-CEA was administered prior to implantation of MC-38-CEA cells followed by administration of anti-PD-1 antibody. CEA-specific T-cell responses were measured by flow cytometry and ELISPOT. Dynamic co-culture of splenocytes with tumor cells was conducted to analyze anti-tumor activities. Tumor infiltration by lymphocytes was measured by IHC. Tumor volume and overall survival were also recorded. Results: Ad-CEA combined with anti-PD-1 antibody showed greater anti-tumor activity compared with either alone. The combination also increased T-cell infiltration but decreased Tregs. Conclusion: Combining Ad-CEA vaccination with anti-PD-1 antibody enhanced anti-tumor activity and immune responses.
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- Vaccination
- Programmed Cell Death 1 Receptor
- Neoplasms, Experimental
- NIH 3T3 Cells
- Mice
- Humans
- Female
- Colonic Neoplasms
- Cell Line, Tumor
- Carcinoembryonic Antigen
Citation
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Vaccination
- Programmed Cell Death 1 Receptor
- Neoplasms, Experimental
- NIH 3T3 Cells
- Mice
- Humans
- Female
- Colonic Neoplasms
- Cell Line, Tumor
- Carcinoembryonic Antigen