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Differential expression of immune related genes in high-grade ovarian serous carcinoma.

Publication ,  Journal Article
Siamakpour-Reihani, S; Cobb, LP; Jiang, C; Zhang, D; Previs, RA; Owzar, K; Nixon, AB; Alvarez Secord, A
Published in: Gynecol Oncol
March 2020

OBJECTIVE: To identify novel immunologic targets and biomarkers associated with overall survival (OS) in high-grade serous ovarian cancer (HGSC). METHODS: In this retrospective study, microarray data from 51 HGSC specimens were analyzed (Affymetrix HG-U133A). A panel of 183 immune/inflammatory response related genes linked to 279 probe sets was constructed a priori and screened. Associations between gene expression and OS were assessed using logrank tests. Multiple testing was addressed within the False Discovery Rate (FDR) framework. For external validation, TCGA Ovarian dataset and five GSE publicly available HGSC datasets were evaluated. RESULTS: In Duke data, 110 probe sets linked to 83 immunologic/inflammatory-related genes were differentially expressed in tumors from long versus short-term HGSC survivors (adjusted p < 0.05). In TCGA, concordant with the results from the Duke discovery cohort, high expression of one probe (IL6R) demonstrated a consistent significance and concordant association with higher expression in long-term HGSC survivors (Duke q-value = 0.022) and improved OS in the TCGA dataset (p-value = 0.015, HR = 0.8). Thirteen genes in GSE14764 (N = 4) and GSE26712 (N = 9) datasets had significant p-values and consistent concordant with Duke Data. Despite the significant associations of gene expression and OS in the individual GSE datasets, in the GSE meta-analysis no genes were consistently concordant and significantly associated with survival. CONCLUSIONS: Evaluation of IL6R expression may be warranted based on higher expression in long-term survivors and association with improved survival in advanced HGSC. The other candidate genes may also be of worthy of further exploration to enhance immuno-oncology drug discovery.

Duke Scholars

Published In

Gynecol Oncol

DOI

EISSN

1095-6859

Publication Date

March 2020

Volume

156

Issue

3

Start / End Page

662 / 668

Location

United States

Related Subject Headings

  • Retrospective Studies
  • Receptors, Interleukin-6
  • Ovarian Neoplasms
  • Oncology & Carcinogenesis
  • Oligonucleotide Array Sequence Analysis
  • Neoplasm Grading
  • Middle Aged
  • Humans
  • Gene Expression
  • Female
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Siamakpour-Reihani, S., Cobb, L. P., Jiang, C., Zhang, D., Previs, R. A., Owzar, K., … Alvarez Secord, A. (2020). Differential expression of immune related genes in high-grade ovarian serous carcinoma. Gynecol Oncol, 156(3), 662–668. https://doi.org/10.1016/j.ygyno.2019.12.019
Siamakpour-Reihani, Sharareh, Lauren Patterson Cobb, Chen Jiang, Dadong Zhang, Rebecca A. Previs, Kouros Owzar, Andrew B. Nixon, and Angeles Alvarez Secord. “Differential expression of immune related genes in high-grade ovarian serous carcinoma.Gynecol Oncol 156, no. 3 (March 2020): 662–68. https://doi.org/10.1016/j.ygyno.2019.12.019.
Siamakpour-Reihani S, Cobb LP, Jiang C, Zhang D, Previs RA, Owzar K, et al. Differential expression of immune related genes in high-grade ovarian serous carcinoma. Gynecol Oncol. 2020 Mar;156(3):662–8.
Siamakpour-Reihani, Sharareh, et al. “Differential expression of immune related genes in high-grade ovarian serous carcinoma.Gynecol Oncol, vol. 156, no. 3, Mar. 2020, pp. 662–68. Pubmed, doi:10.1016/j.ygyno.2019.12.019.
Siamakpour-Reihani S, Cobb LP, Jiang C, Zhang D, Previs RA, Owzar K, Nixon AB, Alvarez Secord A. Differential expression of immune related genes in high-grade ovarian serous carcinoma. Gynecol Oncol. 2020 Mar;156(3):662–668.
Journal cover image

Published In

Gynecol Oncol

DOI

EISSN

1095-6859

Publication Date

March 2020

Volume

156

Issue

3

Start / End Page

662 / 668

Location

United States

Related Subject Headings

  • Retrospective Studies
  • Receptors, Interleukin-6
  • Ovarian Neoplasms
  • Oncology & Carcinogenesis
  • Oligonucleotide Array Sequence Analysis
  • Neoplasm Grading
  • Middle Aged
  • Humans
  • Gene Expression
  • Female