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Circulating MicroRNA Profiling in Non-ST Elevated Coronary Artery Syndrome Highlights Genomic Associations with Serial Platelet Reactivity Measurements.

Publication ,  Journal Article
Becker, KC; Kwee, LC; Neely, ML; Grass, E; Jakubowski, JA; Fox, KAA; White, HD; Gregory, SG; Gurbel, PA; Carvalho, LDP; Becker, RC; Roe, MT ...
Published in: Sci Rep
April 10, 2020

Changes in platelet physiology are associated with simultaneous changes in microRNA concentrations, suggesting a role for microRNA in platelet regulation. Here we investigated potential associations between microRNA and platelet reactivity (PR), a marker of platelet function, in two cohorts following a non-ST elevation acute coronary syndrome (NSTE-ACS) event. First, non-targeted microRNA concentrations and PR were compared in a case (N = 77) control (N = 76) cohort within the larger TRILOGY-ACS trial. MicroRNA significant in this analysis plus CVD-associated microRNAs from the literature were then quantified by targeted rt-PCR in the complete TRILOGY-ACS cohort (N = 878) and compared with matched PR samples. Finally, microRNA significant in the non-targeted & targeted analyses were verified in an independent post NSTE-ACS cohort (N = 96). From the non-targeted analysis, 14 microRNAs were associated with PR (Fold Change: 0.91-1.27, p-value: 0.004-0.05). From the targeted analysis, five microRNAs were associated with PR (Beta: -0.09-0.22, p-value: 0.004-0.05). Of the 19 significant microRNAs, three, miR-15b-5p, miR-93 and miR-126, were consistently associated with PR in the TRILOGY-ACS and independent Singapore post-ACS cohorts, suggesting the measurement of circulating microRNA concentrations may report on dynamic changes in platelet biology following a cardiovascular ischemic event.

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Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

April 10, 2020

Volume

10

Issue

1

Start / End Page

6169

Location

England

Related Subject Headings

  • Singapore
  • Platelet Aggregation
  • Middle Aged
  • Male
  • Humans
  • Gene Expression Profiling
  • Female
  • Circulating MicroRNA
  • Case-Control Studies
  • Blood Platelets
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Becker, K. C., Kwee, L. C., Neely, M. L., Grass, E., Jakubowski, J. A., Fox, K. A. A., … Chan, M. Y. (2020). Circulating MicroRNA Profiling in Non-ST Elevated Coronary Artery Syndrome Highlights Genomic Associations with Serial Platelet Reactivity Measurements. Sci Rep, 10(1), 6169. https://doi.org/10.1038/s41598-020-63263-6
Becker, Kristian C., Lydia Coulter Kwee, Megan L. Neely, Elizabeth Grass, Joseph A. Jakubowski, Keith A. A. Fox, Harvey D. White, et al. “Circulating MicroRNA Profiling in Non-ST Elevated Coronary Artery Syndrome Highlights Genomic Associations with Serial Platelet Reactivity Measurements.Sci Rep 10, no. 1 (April 10, 2020): 6169. https://doi.org/10.1038/s41598-020-63263-6.
Becker KC, Kwee LC, Neely ML, Grass E, Jakubowski JA, Fox KAA, et al. Circulating MicroRNA Profiling in Non-ST Elevated Coronary Artery Syndrome Highlights Genomic Associations with Serial Platelet Reactivity Measurements. Sci Rep. 2020 Apr 10;10(1):6169.
Becker, Kristian C., et al. “Circulating MicroRNA Profiling in Non-ST Elevated Coronary Artery Syndrome Highlights Genomic Associations with Serial Platelet Reactivity Measurements.Sci Rep, vol. 10, no. 1, Apr. 2020, p. 6169. Pubmed, doi:10.1038/s41598-020-63263-6.
Becker KC, Kwee LC, Neely ML, Grass E, Jakubowski JA, Fox KAA, White HD, Gregory SG, Gurbel PA, Carvalho LDP, Becker RC, Magnus Ohman E, Roe MT, Shah SH, Chan MY. Circulating MicroRNA Profiling in Non-ST Elevated Coronary Artery Syndrome Highlights Genomic Associations with Serial Platelet Reactivity Measurements. Sci Rep. 2020 Apr 10;10(1):6169.

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

April 10, 2020

Volume

10

Issue

1

Start / End Page

6169

Location

England

Related Subject Headings

  • Singapore
  • Platelet Aggregation
  • Middle Aged
  • Male
  • Humans
  • Gene Expression Profiling
  • Female
  • Circulating MicroRNA
  • Case-Control Studies
  • Blood Platelets