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Redirecting Vesicular Transport to Improve Nonviral Delivery of Molecular Cargo.

Publication ,  Journal Article
Mao, M; Chang, C-C; Pickar-Oliver, A; Cervia, LD; Wang, L; Ji, J; Liton, PB; Gersbach, CA; Yuan, F
Published in: Adv Biosyst
August 2020

Cell engineering relies heavily on viral vectors for the delivery of molecular cargo into cells due to their superior efficiency compared to nonviral ones. However, viruses are immunogenic and expensive to manufacture, and have limited delivery capacity. Nonviral delivery approaches avoid these limitations but are currently inefficient for clinical applications. This work demonstrates that the efficiency of nonviral delivery of plasmid DNA, mRNA, Sleeping Beauty transposon, and ribonucleoprotein can be significantly enhanced through pretreatment of cells with the nondegradable sugars (NDS), such as sucrose, trehalose, and raffinose. The enhancement is mediated by the incorporation of the NDS into cell membranes, causing enlargement of lysosomes and formation of large (>500 nm) amphisome-like bodies (ALBs). The changes in subcellular structures redirect transport of cargo to ALBs rather than to lysosomes, reducing cargo degradation in cells. The data indicate that pretreatment of cells with NDS is a promising approach to improve nonviral cargo delivery in biomedical applications.

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Published In

Adv Biosyst

DOI

ISSN

2366-7478

Publication Date

August 2020

Volume

4

Issue

8

Start / End Page

e2000059

Location

Germany

Related Subject Headings

  • Trehalose
  • Sucrose
  • Ribonucleoproteins
  • Raffinose
  • RNA, Small Interfering
  • RNA, Messenger
  • Plasmids
  • Microtubule-Associated Proteins
  • Lysosomes
  • Humans
 

Citation

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ICMJE
MLA
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Mao, M., Chang, C.-C., Pickar-Oliver, A., Cervia, L. D., Wang, L., Ji, J., … Yuan, F. (2020). Redirecting Vesicular Transport to Improve Nonviral Delivery of Molecular Cargo. Adv Biosyst, 4(8), e2000059. https://doi.org/10.1002/adbi.202000059
Mao, Mao, Chun-Chi Chang, Adrian Pickar-Oliver, Lisa D. Cervia, Liangli Wang, Jing Ji, Paloma B. Liton, Charles A. Gersbach, and Fan Yuan. “Redirecting Vesicular Transport to Improve Nonviral Delivery of Molecular Cargo.Adv Biosyst 4, no. 8 (August 2020): e2000059. https://doi.org/10.1002/adbi.202000059.
Mao M, Chang C-C, Pickar-Oliver A, Cervia LD, Wang L, Ji J, et al. Redirecting Vesicular Transport to Improve Nonviral Delivery of Molecular Cargo. Adv Biosyst. 2020 Aug;4(8):e2000059.
Mao, Mao, et al. “Redirecting Vesicular Transport to Improve Nonviral Delivery of Molecular Cargo.Adv Biosyst, vol. 4, no. 8, Aug. 2020, p. e2000059. Pubmed, doi:10.1002/adbi.202000059.
Mao M, Chang C-C, Pickar-Oliver A, Cervia LD, Wang L, Ji J, Liton PB, Gersbach CA, Yuan F. Redirecting Vesicular Transport to Improve Nonviral Delivery of Molecular Cargo. Adv Biosyst. 2020 Aug;4(8):e2000059.
Journal cover image

Published In

Adv Biosyst

DOI

ISSN

2366-7478

Publication Date

August 2020

Volume

4

Issue

8

Start / End Page

e2000059

Location

Germany

Related Subject Headings

  • Trehalose
  • Sucrose
  • Ribonucleoproteins
  • Raffinose
  • RNA, Small Interfering
  • RNA, Messenger
  • Plasmids
  • Microtubule-Associated Proteins
  • Lysosomes
  • Humans