Skip to main content

Allo-Specific Humoral Responses: New Methods for Screening Donor-Specific Antibody and Characterization of HLA-Specific Memory B Cells.

Publication ,  Journal Article
Song, S; Manook, M; Kwun, J; Jackson, AM; Knechtle, SJ; Kelsoe, G
Published in: Front Immunol
2021

Antibody-mediated allograft rejection (AMR) causes more kidney transplant failure than any other single cause. AMR is mediated by antibodies recognizing antigens expressed by the graft, and antibodies generated against major histocompatibility complex (MHC) mismatches are especially problematic. Most research directed towards the management of clinical AMR has focused on identifying and characterizing circulating donor-specific HLA antibody (DSA) and optimizing therapies that reduce B-cell activation and/or block antibody secretion by inhibiting plasmacyte survival. Here we describe a novel set of reagents and techniques to allow more specific measurements of MHC sensitization across different animal transplant models. Additionally, we have used these approaches to isolate and clone individual HLA-specific B cells from patients sensitized by pregnancy or transplantation. We have identified and characterized the phenotypes of individual HLA-specific B cells, determined the V(D)J rearrangements of their paired H and L chains, and generated recombinant antibodies to determine affinity and specificity. Knowledge of the BCR genes of individual HLA-specific B cells will allow identification of clonally related B cells by high-throughput sequence analysis of peripheral blood mononuclear cells and permit us to re-construct the origins of HLA-specific B cells and follow their somatic evolution by mutation and selection.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Front Immunol

DOI

EISSN

1664-3224

Publication Date

2021

Volume

12

Start / End Page

705140

Location

Switzerland

Related Subject Headings

  • beta 2-Microglobulin
  • V(D)J Recombination
  • Specific Pathogen-Free Organisms
  • Species Specificity
  • Skin Transplantation
  • RNA, Guide, CRISPR-Cas Systems
  • Models, Animal
  • Mice, Inbred C57BL
  • Mice
  • Macaca mulatta
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Song, S., Manook, M., Kwun, J., Jackson, A. M., Knechtle, S. J., & Kelsoe, G. (2021). Allo-Specific Humoral Responses: New Methods for Screening Donor-Specific Antibody and Characterization of HLA-Specific Memory B Cells. Front Immunol, 12, 705140. https://doi.org/10.3389/fimmu.2021.705140
Song, Shengli, Miriam Manook, Jean Kwun, Annette M. Jackson, Stuart J. Knechtle, and Garnett Kelsoe. “Allo-Specific Humoral Responses: New Methods for Screening Donor-Specific Antibody and Characterization of HLA-Specific Memory B Cells.Front Immunol 12 (2021): 705140. https://doi.org/10.3389/fimmu.2021.705140.
Song S, Manook M, Kwun J, Jackson AM, Knechtle SJ, Kelsoe G. Allo-Specific Humoral Responses: New Methods for Screening Donor-Specific Antibody and Characterization of HLA-Specific Memory B Cells. Front Immunol. 2021;12:705140.
Song, Shengli, et al. “Allo-Specific Humoral Responses: New Methods for Screening Donor-Specific Antibody and Characterization of HLA-Specific Memory B Cells.Front Immunol, vol. 12, 2021, p. 705140. Pubmed, doi:10.3389/fimmu.2021.705140.
Song S, Manook M, Kwun J, Jackson AM, Knechtle SJ, Kelsoe G. Allo-Specific Humoral Responses: New Methods for Screening Donor-Specific Antibody and Characterization of HLA-Specific Memory B Cells. Front Immunol. 2021;12:705140.

Published In

Front Immunol

DOI

EISSN

1664-3224

Publication Date

2021

Volume

12

Start / End Page

705140

Location

Switzerland

Related Subject Headings

  • beta 2-Microglobulin
  • V(D)J Recombination
  • Specific Pathogen-Free Organisms
  • Species Specificity
  • Skin Transplantation
  • RNA, Guide, CRISPR-Cas Systems
  • Models, Animal
  • Mice, Inbred C57BL
  • Mice
  • Macaca mulatta