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Anatomical and functional cardiac PCCT imaging pipeline for characterization of Apolipoprotein E mouse models

Publication ,  Conference
Allphin, AJ; Clark, DP; Badea, A; Badea, CT
Published in: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
January 1, 2022

Non-invasive imaging strategies are essential in understanding the factors associated with the ongoing rise in cardiovascular disease (CVD) and Alzheimer’s disease (AD), and the possible interactions between these diseases. Our scientific premise is based on the role of the APOE gene where APOE4 is considered a risk factor for CVD and AD. This study incorporates the use of novel apolipoprotein E mouse models with a humanized innate immune system, through mNos2 KO and presence of the human NOS2 gene (APOE4/HN and APOE3/HN), together with the more traditional APOE (-/-) mice. We have imaged these models for CVD and AD with a cardiac photon-counting CT (PCCT) imaging pipeline to characterize their cardiac anatomy and function. The pipeline, consisting of contrast enhanced in vivo PCCT imaging, accurate intrinsic cardiac gating, temporally resolved multi-energy iterative reconstruction, spectral decomposition, 3D cardiac segmentation, and ex vivo, high-resolution PCCT imaging allowed for quantitative analysis and comparison of cardiac function as well as identification of anatomical irregularities such as calcified aortic plaques. Our analysis finds no statistically significant differences in cardiac functional metrics or the aortic diameter in these APOE mouse models. Future work will focus on optimizing the image reconstruction to reduce the computation time and on using staining for ex vivo PCCT imaging of the vascular system in these models.

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Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

Publication Date

January 1, 2022

Volume

12036
 

Citation

APA
Chicago
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Allphin, A. J., Clark, D. P., Badea, A., & Badea, C. T. (2022). Anatomical and functional cardiac PCCT imaging pipeline for characterization of Apolipoprotein E mouse models. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 12036). https://doi.org/10.1117/12.2611806
Allphin, A. J., D. P. Clark, A. Badea, and C. T. Badea. “Anatomical and functional cardiac PCCT imaging pipeline for characterization of Apolipoprotein E mouse models.” In Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 12036, 2022. https://doi.org/10.1117/12.2611806.
Allphin AJ, Clark DP, Badea A, Badea CT. Anatomical and functional cardiac PCCT imaging pipeline for characterization of Apolipoprotein E mouse models. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2022.
Allphin, A. J., et al. “Anatomical and functional cardiac PCCT imaging pipeline for characterization of Apolipoprotein E mouse models.” Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 12036, 2022. Scopus, doi:10.1117/12.2611806.
Allphin AJ, Clark DP, Badea A, Badea CT. Anatomical and functional cardiac PCCT imaging pipeline for characterization of Apolipoprotein E mouse models. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2022.

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

Publication Date

January 1, 2022

Volume

12036