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A LGR5 reporter pig model closely resembles human intestine for improved study of stem cells in disease.

Publication ,  Journal Article
Schaaf, CR; Polkoff, KM; Carter, A; Stewart, AS; Sheahan, B; Freund, J; Ginzel, J; Snyder, JC; Roper, J; Piedrahita, JA; Gonzalez, LM
Published in: FASEB J
June 2023

Intestinal epithelial stem cells (ISCs) are responsible for intestinal epithelial barrier renewal; thereby, ISCs play a critical role in intestinal pathophysiology research. While transgenic ISC reporter mice are available, advanced translational studies lack a large animal model. This study validates ISC isolation in a new porcine Leucine Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) reporter line and demonstrates the use of these pigs as a novel colorectal cancer (CRC) model. We applied histology, immunofluorescence, fluorescence-activated cell sorting, flow cytometry, gene expression quantification, and 3D organoid cultures to whole tissue and single cells from the duodenum, jejunum, ileum, and colon of LGR5-H2B-GFP and wild-type pigs. Ileum and colon LGR5-H2B-GFP, healthy human, and murine biopsies were compared by mRNA fluorescent in situ hybridization (FISH). To model CRC, adenomatous polyposis coli (APC) mutation was induced by CRISPR/Cas9 editing in porcine LGR5-H2B-GFP colonoids. Crypt-base, green fluorescent protein (GFP) expressing cells co-localized with ISC biomarkers. LGR5-H2B-GFPhi cells had significantly higher LGR5 expression (p < .01) and enteroid forming efficiency (p < .0001) compared with LGR5-H2B-GFPmed/lo/neg cells. Using FISH, similar LGR5, OLFM4, HOPX, LYZ, and SOX9 expression was identified between human and LGR5-H2B-GFP pig crypt-base cells. LGR5-H2B-GFP/APCnull colonoids had cystic growth in WNT/R-spondin-depleted media and significantly upregulated WNT/β-catenin target gene expression (p < .05). LGR5+ ISCs are reproducibly isolated in LGR5-H2B-GFP pigs and used to model CRC in an organoid platform. The known anatomical and physiologic similarities between pig and human, and those shown by crypt-base FISH, underscore the significance of this novel LGR5-H2B-GFP pig to translational ISC research.

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Published In

FASEB J

DOI

EISSN

1530-6860

Publication Date

June 2023

Volume

37

Issue

6

Start / End Page

e22975

Location

United States

Related Subject Headings

  • Swine
  • Stem Cells
  • Receptors, G-Protein-Coupled
  • Mice
  • Intestines
  • In Situ Hybridization, Fluorescence
  • Ileum
  • Humans
  • Green Fluorescent Proteins
  • Colon
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Schaaf, C. R., Polkoff, K. M., Carter, A., Stewart, A. S., Sheahan, B., Freund, J., … Gonzalez, L. M. (2023). A LGR5 reporter pig model closely resembles human intestine for improved study of stem cells in disease. FASEB J, 37(6), e22975. https://doi.org/10.1096/fj.202300223R
Schaaf, Cecilia R., Kathryn M. Polkoff, Amber Carter, Amy S. Stewart, Breanna Sheahan, John Freund, Joshua Ginzel, et al. “A LGR5 reporter pig model closely resembles human intestine for improved study of stem cells in disease.FASEB J 37, no. 6 (June 2023): e22975. https://doi.org/10.1096/fj.202300223R.
Schaaf CR, Polkoff KM, Carter A, Stewart AS, Sheahan B, Freund J, et al. A LGR5 reporter pig model closely resembles human intestine for improved study of stem cells in disease. FASEB J. 2023 Jun;37(6):e22975.
Schaaf, Cecilia R., et al. “A LGR5 reporter pig model closely resembles human intestine for improved study of stem cells in disease.FASEB J, vol. 37, no. 6, June 2023, p. e22975. Pubmed, doi:10.1096/fj.202300223R.
Schaaf CR, Polkoff KM, Carter A, Stewart AS, Sheahan B, Freund J, Ginzel J, Snyder JC, Roper J, Piedrahita JA, Gonzalez LM. A LGR5 reporter pig model closely resembles human intestine for improved study of stem cells in disease. FASEB J. 2023 Jun;37(6):e22975.

Published In

FASEB J

DOI

EISSN

1530-6860

Publication Date

June 2023

Volume

37

Issue

6

Start / End Page

e22975

Location

United States

Related Subject Headings

  • Swine
  • Stem Cells
  • Receptors, G-Protein-Coupled
  • Mice
  • Intestines
  • In Situ Hybridization, Fluorescence
  • Ileum
  • Humans
  • Green Fluorescent Proteins
  • Colon