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Comprehensive characterization of pathogenic synovial fluid extracellular vesicles from knee osteoarthritis.

Publication ,  Journal Article
Zhang, X; Ma, S; Naz, SI; Jain, V; Soderblom, EJ; Aliferis, C; Kraus, VB
Published in: Clin Immunol
December 2023

Synovial fluid (SF) extracellular vesicles (EVs) play a pathogenic role in osteoarthritis (OA). However, the surface markers, cell and tissue origins, and effectors of these EVs are largely unknown. We found that SF EVs contained 692 peptides that were positively associated with knee radiographic OA severity; 57.4% of these pathogenic peptides were from 46 proteins of the immune system, predominantly the innate immune system. CSPG4, BGN, NRP1, and CD109 are the major surface markers of pathogenic SF EVs. Genes encoding surface marker CSPG4 and CD109 were highly expressed by chondrocytes from damaged cartilage, while VISG4, MARCO, CD163 and NRP1 were enriched in the synovial immune cells. The frequency of CSPG4+ and VSIG4+ EV subpopulations in OA SF was high. We conclude that pathogenic SF EVs carry knee OA severity-associated proteins and specific surface markers, which could be developed as a new source of diagnostic biomarkers or therapeutic targets in OA.

Duke Scholars

Published In

Clin Immunol

DOI

EISSN

1521-7035

Publication Date

December 2023

Volume

257

Start / End Page

109812

Location

United States

Related Subject Headings

  • Synovial Fluid
  • Peptides
  • Osteoarthritis, Knee
  • Immunology
  • Humans
  • Extracellular Vesicles
  • Biomarkers
  • 3204 Immunology
  • 1107 Immunology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Zhang, X., Ma, S., Naz, S. I., Jain, V., Soderblom, E. J., Aliferis, C., & Kraus, V. B. (2023). Comprehensive characterization of pathogenic synovial fluid extracellular vesicles from knee osteoarthritis. Clin Immunol, 257, 109812. https://doi.org/10.1016/j.clim.2023.109812
Zhang, Xin, Sisi Ma, Syeda Iffat Naz, Vaibhav Jain, Erik J. Soderblom, Constantin Aliferis, and Virginia Byers Kraus. “Comprehensive characterization of pathogenic synovial fluid extracellular vesicles from knee osteoarthritis.Clin Immunol 257 (December 2023): 109812. https://doi.org/10.1016/j.clim.2023.109812.
Zhang X, Ma S, Naz SI, Jain V, Soderblom EJ, Aliferis C, et al. Comprehensive characterization of pathogenic synovial fluid extracellular vesicles from knee osteoarthritis. Clin Immunol. 2023 Dec;257:109812.
Zhang, Xin, et al. “Comprehensive characterization of pathogenic synovial fluid extracellular vesicles from knee osteoarthritis.Clin Immunol, vol. 257, Dec. 2023, p. 109812. Pubmed, doi:10.1016/j.clim.2023.109812.
Zhang X, Ma S, Naz SI, Jain V, Soderblom EJ, Aliferis C, Kraus VB. Comprehensive characterization of pathogenic synovial fluid extracellular vesicles from knee osteoarthritis. Clin Immunol. 2023 Dec;257:109812.
Journal cover image

Published In

Clin Immunol

DOI

EISSN

1521-7035

Publication Date

December 2023

Volume

257

Start / End Page

109812

Location

United States

Related Subject Headings

  • Synovial Fluid
  • Peptides
  • Osteoarthritis, Knee
  • Immunology
  • Humans
  • Extracellular Vesicles
  • Biomarkers
  • 3204 Immunology
  • 1107 Immunology