FIGURE 6 from Overcoming Xenoantigen Immunity to Enable Cellular Tracking and Gene Regulation with Immune-competent “NoGlow” Mice
Trotter, TN; Wilson, A; McBane, J; Dagotto, CE; Yang, X-Y; Wei, J-P; Lei, G; Thrash, H; Snyder, JC; Lyerly, HK; Hartman, ZC
<p>NoGlow mice reveal unappreciated metastatic dynamics independent of the primary tumor. <b>A,</b> Tumor growth of triple transgenic (3 ×) B16-F10 cells (10<sup>5</sup>) after subcutaneous implantation into the flank of WT C57Bl/6 (<i>n</i> = 2), CAG-driven Full-body WT GFP/Luc expressing mice (<i>n</i> = 3), CMV cre littermates positive (<i>n</i> = 8) or negative (<i>n</i> = 5) for the NoGlow construct, or SCID beige (<i>n</i> = 5) mice. <b>B,</b> Bioluminescence imaging of lungs at the time of euthanasia for each mouse from A. Robust signal is detected only in NoGlow+ lungs. *Indicates no detectable primary tumor at the end of experiment. <b>C,</b> Bioluminescence intensity of individual lungs from B. NoGlow+ lungs contained significantly more positive Luc signal than SCID, WT, or NoGlow− animals. <b>D,</b> Plot of tumor volume at time of euthanasia (x) by total lung radiance (y) for individual animals from A. <i>P</i> values were determined by one-way ANOVA with Tukey correction. All <i>P</i> values represent mean ± SEM.</p>
