FIGURE 4 from Overcoming Xenoantigen Immunity to Enable Cellular Tracking and Gene Regulation with Immune-competent “NoGlow” Mice

<p>NoGlow mice reveal the impact of sex and tissue distribution on immune tolerance. <b>A,</b> Male WT (<i>n</i> = 3), CAG Luc-GFP (<i>n</i> = 3), and NoGlow+ (<i>n</i> = 6) or NoGlow− (<i>n</i> = 10) littermates were vaccinated with an Ad encoding WT GFP-Luc and serum was collected after two weeks for anti-Luc or anti-GFP responses. Unvaccinated naïve WT (<i>n</i> = 2) animals were used as baseline controls. <b>B,</b> Luc staining showing protein expression in mammary epithelium of female MMTV Cre NoGlow+ or NoGlow− littermates. <b>C,</b> Luc staining showing protein expression in prostate epithelium of male Pbsn Cre NoGlow+ or NoGlow− littermates. <b>D,</b> Luc staining demonstrating expression in acini and islets in pancreases of male Pdx1 Cre NoGlow+ or NoGlow− littermates. <b>E–G,</b> MMTV Cre (E; <i>n</i> = 4 NoGlow- F, <i>n</i> = 5 NoGlow+ F, <i>n</i> = 2 NoGlow- M, <i>n</i> = 7 NoGlow+ M, <i>n</i> = 3 naïve), Pbsn Cre (F; <i>n</i> = 6 NoGlow- F, <i>n</i> = 6 NoGlow+ F, <i>n</i> = 4 NoGlow- M, <i>n</i> = 8 NoGlow+ M, <i>n</i> = 1 naïve), or Pdx1 Cre (G; <i>n</i> = 2 NoGlow- F, <i>n</i> = 4 NoGlow+ F, <i>n</i> = 4 NoGlow- M, <i>n</i> = 5 NoGlow+ M, <i>n</i> = 2 naïve) littermates were vaccinated with an Ad encoding WT eGFP and Luc. Serum antibody responses to Luc and GFP were determined by ELISA two weeks postvaccination. <i>P</i> values for A, E, F, G are displayed at 1:50 and determined by two-way ANOVA with Tukey correction. <b>H,</b> Normalized ratio of serum anti-Luc or -GFP antibody responses in independent NoGlow+ versus mean of NoGlow− littermates across two independent experiments demonstrating highest tolerance in CMV Cre and Pdx1 Cre animals. <i>P</i> values were determined by one-way ANOVA with Tukey correction. All <i>P</i> values represent mean ± SEM.</p>

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