FIGURE 2 from Overcoming Xenoantigen Immunity to Enable Cellular Tracking and Gene Regulation with Immune-competent “NoGlow” Mice

<p>Mutant GFP and luciferase induce immune responses comparable with wild-type (WT) proteins. <b>A,</b> WT male Balb/c mice were vaccinated with Ad encoding WT or mutant copies of GFP (ΔT64; <i>n</i> = 5/group) and serum antibody responses were measured after 3 weeks. Mice showed equivalent anti-GFP responses whether vaccinated with WT or mutant GFP. <b>B,</b> WT female C57Bl/6 mice were vaccinated with Ad-GFP-Luc WT or Ad-Luc mutant (G315A; <i>n</i> = 5/group) and serum antibody responses were measured after 3 weeks revealing similar levels of anti-Luc antibodies. <i>P</i> values for A, B are at 1:50 dilution by two-way ANOVA with Tukey multiple comparisons test. <b>C,</b> Male WT Balb/c mice were vaccinated with Ad encoding WT or mutant GFP (ΔT64) or Luc (H245R) and anti-GFP or -Luc T-cell responses in the spleens were detected after 3 weeks via ELISpot. Both WT and mutant forms of GFP and Luc were able to elicit robust T-cell responses compared with mice naïve for the respective antigen. <i>P</i> values were determined by two-way ANOVA with Tukey <i>post hoc</i> analysis. <b>D,</b> E0771 GFP-Luc cells were implanted in MFPs of female WT C57Bl/6 mice and were vaccinated with Ad encoding WT GFP, mutant GFP (ΔT64), WT Luc, mutant Luc (ΔH245), or LacZ control (<i>n</i> = 5 per group) after three days. Compared with LacZ control vaccination, GFP WT/mutant, or Luc WT/mutant vaccines resulted in a comparable reduction in tumor growth. <i>P</i> values are at time of euthanasia by two-way ANOVA with Tukey multiple comparisons test. All <i>P</i> values represent mean ± SEM.</p>

Duke Scholars

Author Herbert Kim Lyerly Surgery, Surgical Sciences

Author Joshua Clair Snyder Surgery, Surgical Sciences

Author Zachary Conrad Hartman Surgery, Surgical Sciences