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Three-dimensional diffractive acoustic tomography.

Publication ,  Journal Article
Menozzi, L; Vu, T; Canning, AJ; Rawtani, H; Taboada, C; Abi Antoun, ME; Ma, C; Delia, J; Nguyen, VT; Cho, S-W; Chen, J; Charity, T; Xu, Y ...
Published in: Nat Commun
January 29, 2025

Acoustically probing biological tissues with light or sound, photoacoustic and ultrasound imaging can provide anatomical, functional, and/or molecular information at depths far beyond the optical diffusion limit. However, most photoacoustic and ultrasound imaging systems rely on linear-array transducers with elevational focusing and are limited to two-dimensional imaging with anisotropic resolutions. Here, we present three-dimensional diffractive acoustic tomography (3D-DAT), which uses an off-the-shelf linear-array transducer with single-slit acoustic diffraction. Without jeopardizing its accessibility by general users, 3D-DAT has achieved simultaneous 3D photoacoustic and ultrasound imaging with optimal imaging performance in deep tissues, providing near-isotropic resolutions, high imaging speed, and a large field-of-view, as well as enhanced quantitative accuracy and detection sensitivity. Moreover, powered by the fast focal line volumetric reconstruction, 3D-DAT has achieved 50-fold faster reconstruction times than traditional photoacoustic imaging reconstruction. Using 3D-DAT on small animal models, we mapped the distribution of the biliverdin-binding serpin complex in glassfrogs, tracked gold nanoparticle accumulation in a mouse tumor model, imaged genetically-encoded photoswitchable tumors, and investigated polyfluoroalkyl substances exposure on developing embryos. With its enhanced imaging performance and high accessibility, 3D-DAT may find broad applications in fundamental life sciences and biomedical research.

Duke Scholars

Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

January 29, 2025

Volume

16

Issue

1

Start / End Page

1149

Location

England

Related Subject Headings

  • Ultrasonography
  • Transducers
  • Tomography
  • Serpins
  • Photoacoustic Techniques
  • Mice
  • Imaging, Three-Dimensional
  • Gold
  • Animals
  • Acoustics
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Menozzi, L., Vu, T., Canning, A. J., Rawtani, H., Taboada, C., Abi Antoun, M. E., … Yao, J. (2025). Three-dimensional diffractive acoustic tomography. Nat Commun, 16(1), 1149. https://doi.org/10.1038/s41467-025-56435-3
Menozzi, Luca, Tri Vu, Aidan J. Canning, Harshal Rawtani, Carlos Taboada, Marie Elise Abi Antoun, Chenshuo Ma, et al. “Three-dimensional diffractive acoustic tomography.Nat Commun 16, no. 1 (January 29, 2025): 1149. https://doi.org/10.1038/s41467-025-56435-3.
Menozzi L, Vu T, Canning AJ, Rawtani H, Taboada C, Abi Antoun ME, et al. Three-dimensional diffractive acoustic tomography. Nat Commun. 2025 Jan 29;16(1):1149.
Menozzi, Luca, et al. “Three-dimensional diffractive acoustic tomography.Nat Commun, vol. 16, no. 1, Jan. 2025, p. 1149. Pubmed, doi:10.1038/s41467-025-56435-3.
Menozzi L, Vu T, Canning AJ, Rawtani H, Taboada C, Abi Antoun ME, Ma C, Delia J, Nguyen VT, Cho S-W, Chen J, Charity T, Xu Y, Tran P, Xia J, Palmer GM, Vo-Dinh T, Feng L, Yao J. Three-dimensional diffractive acoustic tomography. Nat Commun. 2025 Jan 29;16(1):1149.

Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

January 29, 2025

Volume

16

Issue

1

Start / End Page

1149

Location

England

Related Subject Headings

  • Ultrasonography
  • Transducers
  • Tomography
  • Serpins
  • Photoacoustic Techniques
  • Mice
  • Imaging, Three-Dimensional
  • Gold
  • Animals
  • Acoustics