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APOE genotype-specific differences in human and mouse macrophage nitric oxide production.

Publication ,  Journal Article
Colton, CA; Needham, LK; Brown, C; Cook, D; Rasheed, K; Burke, JR; Strittmatter, WJ; Schmechel, DE; Vitek, MP
Published in: J Neuroimmunol
February 2004
Published version (DOI) Link to item

Individuals expressing an APOE4 genotype demonstrate increased Alzheimer's disease (AD) neuropathology and a decreased onset age. The APOE4 gene may act by modulating the CNS immune response. Using human monocyte-derived macrophages (MDM), we show a significantly greater increase in NO production during immune activation in MDM from APOE4 AD patients compared to normal, age-matched individuals or to AD patients with an APOE 3/3 genotype. Microglia and peritoneal macrophages from APOE4 targeted replacement mice demonstrate a similar increase in NO compared to the APOE3 targeted replacement mice. The enhanced macrophage responsiveness and the increased production of NO in APOE4 AD patients may predispose the CNS to an increased potential for nitration and nitrosation, consistent with the redox imbalance and neuroinflammatory state seen in AD.

Duke Scholars

Carol Anne Colton
Author Carol Anne Colton Neurology, Behavioral Neurology
James Robert Burke
Author James Robert Burke Neurology, Behavioral Neurology
Warren James Strittmatter
Author Warren James Strittmatter Neurology, Behavioral Neurology
Michael P. Vitek
Author Michael P. Vitek Neurology, Behavioral Neurology

Published In

J Neuroimmunol

DOI

10.1016/j.jneuroim.2003.10.015

ISSN

0165-5728

Publication Date

February 2004

Volume

147

Issue

1-2

Start / End Page

62 / 67

Location

Netherlands

Related Subject Headings

  • Species Specificity
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Nitric Oxide Synthase Type II
  • Nitric Oxide Synthase
  • Nitric Oxide
  • Neurology & Neurosurgery
  • Monocytes
  • Middle Aged
  • Microglia
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Colton, C. A., Needham, L. K., Brown, C., Cook, D., Rasheed, K., Burke, J. R., … Vitek, M. P. (2004). APOE genotype-specific differences in human and mouse macrophage nitric oxide production. J Neuroimmunol, 147(1–2), 62–67. https://doi.org/10.1016/j.jneuroim.2003.10.015
Colton, Carol A., Leila K. Needham, Candice Brown, Danielle Cook, Karima Rasheed, James R. Burke, Warren J. Strittmatter, Donald E. Schmechel, and Michael P. Vitek. “APOE genotype-specific differences in human and mouse macrophage nitric oxide production.” J Neuroimmunol 147, no. 1–2 (February 2004): 62–67. https://doi.org/10.1016/j.jneuroim.2003.10.015.
Colton CA, Needham LK, Brown C, Cook D, Rasheed K, Burke JR, et al. APOE genotype-specific differences in human and mouse macrophage nitric oxide production. J Neuroimmunol. 2004 Feb;147(1–2):62–7.
Colton, Carol A., et al. “APOE genotype-specific differences in human and mouse macrophage nitric oxide production.” J Neuroimmunol, vol. 147, no. 1–2, Feb. 2004, pp. 62–67. Pubmed, doi:10.1016/j.jneuroim.2003.10.015.
Colton CA, Needham LK, Brown C, Cook D, Rasheed K, Burke JR, Strittmatter WJ, Schmechel DE, Vitek MP. APOE genotype-specific differences in human and mouse macrophage nitric oxide production. J Neuroimmunol. 2004 Feb;147(1–2):62–67.
Journal cover image

Published In

J Neuroimmunol

DOI

10.1016/j.jneuroim.2003.10.015

ISSN

0165-5728

Publication Date

February 2004

Volume

147

Issue

1-2

Start / End Page

62 / 67

Location

Netherlands

Related Subject Headings

  • Species Specificity
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Nitric Oxide Synthase Type II
  • Nitric Oxide Synthase
  • Nitric Oxide
  • Neurology & Neurosurgery
  • Monocytes
  • Middle Aged
  • Microglia