Scholars@Duke publication: Depletion of human regulatory T cells.

Depletion of human regulatory T cells.

Publication , Journal Article

Hobeika, AC; Morse, MA; Osada, T; Peplinski, S; Lyerly, HK; Clay, TM

Published in: Methods Mol Biol

2011

Regulatory T cells (Treg) have become increasingly relevant in the study of human disease including cancer. Treg cells have been shown to inhibit anti-tumor immune responses, and elevated Treg levels have been associated with certain types of cancer. Similarly, depletion of Tregs by various methods can also enhance anti-tumor immune responses. We have found a prevalence of Treg in cancer patients when compared to normal volunteers. In addition, we have shown that the depletion of Treg using the IL-2 fusion protein denileukin diftitox decreased Treg function and increased antigen-specific T cell response to a cancer vaccine. These results indicate the potential for combining Treg depletion with anti-cancer vaccines to enhance tumor antigen-specific immune responses and the need to explore the dose and schedule of Treg depletion strategies in optimizing vaccine efforts.

Duke Scholars

Author Amy Claudine Hobeika Surgery, Surgical Sciences

Author Michael Aaron Morse Medicine, Medical Oncology

Author Takuya Osada Surgery, Surgical Sciences

Author Herbert Kim Lyerly Surgery, Surgical Sciences

Published In

Methods Mol Biol

DOI

10.1007/978-1-61737-979-6_14

EISSN

1940-6029

Publication Date

2011

Volume

707

Start / End Page

219 / 231

Location

United States

Related Subject Headings

T-Lymphocytes, Regulatory
Recombinant Fusion Proteins
Neoplasms
Lymphocyte Depletion
Interleukin-2
Humans
Diphtheria Toxin
Developmental Biology
Antigens, Neoplasm
3404 Medicinal and biomolecular chemistry

Citation

APA

Chicago

ICMJE

MLA

NLM

Hobeika, A. C., Morse, M. A., Osada, T., Peplinski, S., Lyerly, H. K., & Clay, T. M. (2011). Depletion of human regulatory T cells. Methods Mol Biol, 707, 219–231. https://doi.org/10.1007/978-1-61737-979-6_14

Hobeika, Amy C., Michael A. Morse, Takuya Osada, Sharon Peplinski, H Kim Lyerly, and Timothy M. Clay. “Depletion of human regulatory T cells.” Methods Mol Biol 707 (2011): 219–31. https://doi.org/10.1007/978-1-61737-979-6_14.

Hobeika AC, Morse MA, Osada T, Peplinski S, Lyerly HK, Clay TM. Depletion of human regulatory T cells. Methods Mol Biol. 2011;707:219–31.

Hobeika, Amy C., et al. “Depletion of human regulatory T cells.” Methods Mol Biol, vol. 707, 2011, pp. 219–31. Pubmed, doi:10.1007/978-1-61737-979-6_14.

Hobeika AC, Morse MA, Osada T, Peplinski S, Lyerly HK, Clay TM. Depletion of human regulatory T cells. Methods Mol Biol. 2011;707:219–231.

Published In

Methods Mol Biol

DOI

10.1007/978-1-61737-979-6_14

EISSN

1940-6029

Publication Date

2011

Volume

707

Start / End Page

219 / 231

Location

United States

Related Subject Headings

T-Lymphocytes, Regulatory
Recombinant Fusion Proteins
Neoplasms
Lymphocyte Depletion
Interleukin-2
Humans
Diphtheria Toxin
Developmental Biology
Antigens, Neoplasm
3404 Medicinal and biomolecular chemistry