Skip to main content
construction release_alert
The Scholars Team is working with OIT to resolve some issues with the Scholars search index
cancel

The human thymic microenvironment during organ culture.

Publication ,  Journal Article
Markert, ML; Watson, TJ; Kaplan, I; Hale, LP; Haynes, BF
Published in: Clin Immunol Immunopathol
January 1997

Cultured human thymic tissue has been transplanted into many patients with T cell dysfunction; however, little is known about the effect of in vitro culture on thymic tissue. Human postnatal thymic organ cultures were established in vitro to study the growth potential of the thymic epithelium and the expression of intracellular and surface antigens with time in culture. Marked depletion of bone marrow-derived cells was observed within 3 weeks of initiation of organ cultures although some viable CD3+ cells could still be detected. Thymic epithelial cells in in vitro explants continued to express MHC class I and class II antigens as well as cytokeratins. Thymic epithelial cells within cultured thymic organ slices maintained their postnatal growth potential, in that cytokeratin-positive epithelial monolayers could be established in vitro from these thymic slices up to 12 weeks after initiation of organ culture. Thus, thymic explants remained viable in culture and could potentially be used to reconstitute immunity in T cell deficient patients.

Duke Scholars

Published In

Clin Immunol Immunopathol

DOI

ISSN

0090-1229

Publication Date

January 1997

Volume

82

Issue

1

Start / End Page

26 / 36

Location

United States

Related Subject Headings

  • Time Factors
  • Thymus Gland
  • T-Lymphocytes
  • Organ Culture Techniques
  • Leukocyte Common Antigens
  • Keratins
  • Immunology
  • Humans
  • Epithelium
  • Epithelial Cells
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Markert, M. L., Watson, T. J., Kaplan, I., Hale, L. P., & Haynes, B. F. (1997). The human thymic microenvironment during organ culture. Clin Immunol Immunopathol, 82(1), 26–36. https://doi.org/10.1006/clin.1996.4266
Markert, M. L., T. J. Watson, I. Kaplan, L. P. Hale, and B. F. Haynes. “The human thymic microenvironment during organ culture.Clin Immunol Immunopathol 82, no. 1 (January 1997): 26–36. https://doi.org/10.1006/clin.1996.4266.
Markert ML, Watson TJ, Kaplan I, Hale LP, Haynes BF. The human thymic microenvironment during organ culture. Clin Immunol Immunopathol. 1997 Jan;82(1):26–36.
Markert, M. L., et al. “The human thymic microenvironment during organ culture.Clin Immunol Immunopathol, vol. 82, no. 1, Jan. 1997, pp. 26–36. Pubmed, doi:10.1006/clin.1996.4266.
Markert ML, Watson TJ, Kaplan I, Hale LP, Haynes BF. The human thymic microenvironment during organ culture. Clin Immunol Immunopathol. 1997 Jan;82(1):26–36.

Published In

Clin Immunol Immunopathol

DOI

ISSN

0090-1229

Publication Date

January 1997

Volume

82

Issue

1

Start / End Page

26 / 36

Location

United States

Related Subject Headings

  • Time Factors
  • Thymus Gland
  • T-Lymphocytes
  • Organ Culture Techniques
  • Leukocyte Common Antigens
  • Keratins
  • Immunology
  • Humans
  • Epithelium
  • Epithelial Cells