Skip to main content

Oxidative stress-induced expression and modulation of Phosphatase of Regenerating Liver-1 (PRL-1) in mammalian retina.

Publication ,  Journal Article
Yu, L; Kelly, U; Ebright, JN; Malek, G; Saloupis, P; Rickman, DW; McKay, BS; Arshavsky, VY; Bowes Rickman, C
Published in: Biochim Biophys Acta
September 2007

The phosphatase of regenerating liver-1, PRL-1, gene was detected in a screen for foveal cone photoreceptor-associated genes. It encodes a small protein tyrosine phosphatase that was previously immunolocalized to the photoreceptors in primate retina. Here we report that in cones and cone-derived cultured cells both PRL-1 activity and PRL-1 gene expression are modulated under oxidative stress. Oxidation reversibly inhibited the phosphatase activity of PRL-1 due to the formation of an intramolecular disulfide bridge between Cys104 within the active site and another conserved Cys, Cys49. This modulation was observed in vitro, in cell culture and in isolated retinas exposed to hydrogen peroxide. The same treatment caused a rapid increase in PRL-1 expression levels in cultured cells which could be blocked by the protein translation inhibitor, cycloheximide. Increased PRL-1 expression was also observed in living rats subjected to constant light exposure inducing photooxidative stress. We further demonstrated that both oxidation and overexpression of PRL-1 upon oxidative stress are greatly enhanced by inhibition of the glutathione system responsible for cellular redox regulation. These findings suggest that PRL-1 is a molecular component of the photoreceptor's response to oxidative stress acting upstream of the glutathione system.

Duke Scholars

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

September 2007

Volume

1773

Issue

9

Start / End Page

1473 / 1482

Location

Netherlands

Related Subject Headings

  • Swine
  • Reverse Transcriptase Polymerase Chain Reaction
  • Retinal Cone Photoreceptor Cells
  • Retina
  • Reducing Agents
  • Recombinant Proteins
  • Rats, Sprague-Dawley
  • Rats
  • Protein Tyrosine Phosphatases
  • Protein Synthesis Inhibitors
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Yu, L., Kelly, U., Ebright, J. N., Malek, G., Saloupis, P., Rickman, D. W., … Bowes Rickman, C. (2007). Oxidative stress-induced expression and modulation of Phosphatase of Regenerating Liver-1 (PRL-1) in mammalian retina. Biochim Biophys Acta, 1773(9), 1473–1482. https://doi.org/10.1016/j.bbamcr.2007.06.005
Yu, Ling, Una Kelly, Jessica N. Ebright, Goldis Malek, Peter Saloupis, Dennis W. Rickman, Brian S. McKay, Vadim Y. Arshavsky, and Catherine Bowes Rickman. “Oxidative stress-induced expression and modulation of Phosphatase of Regenerating Liver-1 (PRL-1) in mammalian retina.Biochim Biophys Acta 1773, no. 9 (September 2007): 1473–82. https://doi.org/10.1016/j.bbamcr.2007.06.005.
Yu L, Kelly U, Ebright JN, Malek G, Saloupis P, Rickman DW, et al. Oxidative stress-induced expression and modulation of Phosphatase of Regenerating Liver-1 (PRL-1) in mammalian retina. Biochim Biophys Acta. 2007 Sep;1773(9):1473–82.
Yu, Ling, et al. “Oxidative stress-induced expression and modulation of Phosphatase of Regenerating Liver-1 (PRL-1) in mammalian retina.Biochim Biophys Acta, vol. 1773, no. 9, Sept. 2007, pp. 1473–82. Pubmed, doi:10.1016/j.bbamcr.2007.06.005.
Yu L, Kelly U, Ebright JN, Malek G, Saloupis P, Rickman DW, McKay BS, Arshavsky VY, Bowes Rickman C. Oxidative stress-induced expression and modulation of Phosphatase of Regenerating Liver-1 (PRL-1) in mammalian retina. Biochim Biophys Acta. 2007 Sep;1773(9):1473–1482.

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

September 2007

Volume

1773

Issue

9

Start / End Page

1473 / 1482

Location

Netherlands

Related Subject Headings

  • Swine
  • Reverse Transcriptase Polymerase Chain Reaction
  • Retinal Cone Photoreceptor Cells
  • Retina
  • Reducing Agents
  • Recombinant Proteins
  • Rats, Sprague-Dawley
  • Rats
  • Protein Tyrosine Phosphatases
  • Protein Synthesis Inhibitors