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Mast cells augment adaptive immunity by orchestrating dendritic cell trafficking through infected tissues.

Publication ,  Journal Article
Shelburne, CP; Nakano, H; St John, AL; Chan, C; McLachlan, JB; Gunn, MD; Staats, HF; Abraham, SN
Published in: Cell Host Microbe
October 22, 2009
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Mast cells (MCs) are best known for eliciting harmful reactions, mostly after primary immunity has been established. Here, we report that, during footpad infection with E. coli in MC-deficient mice, as compared to their MC-sufficient counterparts, the serum antibody response is significantly diminished and less protective following passive immunization in a urinary tract infection (UTI) model in wild-type mice. MCs were found to recruit large numbers of dendritic cells (DCs) into the infected tissue site, which eventually migrated into draining lymph nodes (DLNs) during a prolonged time course. This pattern of trafficking was facilitated by MC-generated TNF, which increased the expression of E-selectin on local blood vessels. Antibody blockade of E-selectin inhibited DC recruitment into the site of infection and DLNs and consequently impaired the primary humoral immune response. Thus, during infection, resident MCs contribute to the primary protective adaptive response through recruitment of DCs from the circulation into infected sites.

Duke Scholars

Ashley St John
Author Ashley St John Pathology
Michael Dee Gunn
Author Michael Dee Gunn Medicine, Cardiology
Herman Ford Staats
Author Herman Ford Staats Pathology
Soman Ninan Abraham
Author Soman Ninan Abraham Pathology
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Published In

Cell Host Microbe

DOI

10.1016/j.chom.2009.09.004

EISSN

1934-6069

Publication Date

October 22, 2009

Volume

6

Issue

4

Start / End Page

331 / 342

Location

United States

Related Subject Headings

  • Urinary Tract Infections
  • Tumor Necrosis Factor-alpha
  • Soft Tissue Infections
  • Mice, Inbred C57BL
  • Mice
  • Mast Cells
  • Male
  • Immunology
  • Female
  • Escherichia coli Infections
 

Citation

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Shelburne, C. P., Nakano, H., St John, A. L., Chan, C., McLachlan, J. B., Gunn, M. D., … Abraham, S. N. (2009). Mast cells augment adaptive immunity by orchestrating dendritic cell trafficking through infected tissues. Cell Host Microbe, 6(4), 331–342. https://doi.org/10.1016/j.chom.2009.09.004
Shelburne, Christopher P., Hideki Nakano, Ashley L. St John, Cheryl Chan, James B. McLachlan, Michael D. Gunn, Herman F. Staats, and Soman N. Abraham. “Mast cells augment adaptive immunity by orchestrating dendritic cell trafficking through infected tissues.” Cell Host Microbe 6, no. 4 (October 22, 2009): 331–42. https://doi.org/10.1016/j.chom.2009.09.004.
Shelburne CP, Nakano H, St John AL, Chan C, McLachlan JB, Gunn MD, et al. Mast cells augment adaptive immunity by orchestrating dendritic cell trafficking through infected tissues. Cell Host Microbe. 2009 Oct 22;6(4):331–42.
Shelburne, Christopher P., et al. “Mast cells augment adaptive immunity by orchestrating dendritic cell trafficking through infected tissues.” Cell Host Microbe, vol. 6, no. 4, Oct. 2009, pp. 331–42. Pubmed, doi:10.1016/j.chom.2009.09.004.
Shelburne CP, Nakano H, St John AL, Chan C, McLachlan JB, Gunn MD, Staats HF, Abraham SN. Mast cells augment adaptive immunity by orchestrating dendritic cell trafficking through infected tissues. Cell Host Microbe. 2009 Oct 22;6(4):331–342.
Journal cover image

Published In

Cell Host Microbe

DOI

10.1016/j.chom.2009.09.004

EISSN

1934-6069

Publication Date

October 22, 2009

Volume

6

Issue

4

Start / End Page

331 / 342

Location

United States

Related Subject Headings

  • Urinary Tract Infections
  • Tumor Necrosis Factor-alpha
  • Soft Tissue Infections
  • Mice, Inbred C57BL
  • Mice
  • Mast Cells
  • Male
  • Immunology
  • Female
  • Escherichia coli Infections