The homeodomain protein HOXB13 regulates the cellular response to androgens.
HOXB13 is a member of the homeodomain family of sequence-specific transcription factors and, together with the androgen receptor (AR), plays a critical role in the normal development of the prostate gland. We demonstrate here that, in prostate cancer cells, HOXB13 is a key determinant of the response to androgens. Specifically, it was determined that HOXB13 interacts with the DNA-binding domain of AR and inhibits the transcription of genes that contain an androgen-response element (ARE). In contrast, the AR:HOXB13 complex confers androgen responsiveness to promoters that contain a specific HOXB13-response element. Further, HOXB13 and AR synergize to enhance the transcription of genes that contain a HOX element juxtaposed to an ARE. The profound effects of HOXB13 knockdown on androgen-regulated proliferation, migration, and lipogenesis in prostate cancer cells highlight the importance of the observed changes in gene expression.
Duke Scholars
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- Transfection
- Reverse Transcriptase Polymerase Chain Reaction
- Response Elements
- Recombinant Fusion Proteins
- Receptors, Androgen
- RNA, Small Interfering
- Protein Binding
- Prostatic Neoplasms
- Metribolone
- Male
Citation
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Transfection
- Reverse Transcriptase Polymerase Chain Reaction
- Response Elements
- Recombinant Fusion Proteins
- Receptors, Androgen
- RNA, Small Interfering
- Protein Binding
- Prostatic Neoplasms
- Metribolone
- Male