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Overexpression of TNNI3K, a cardiac-specific MAPKKK, promotes cardiac dysfunction.

Publication ,  Journal Article
Tang, H; Xiao, K; Mao, L; Rockman, HA; Marchuk, DA
Published in: J Mol Cell Cardiol
January 2013

Cardiac troponin I-interacting kinase (TNNI3K) is a cardiac-specific kinase whose biological function remains largely unknown. We have recently shown that TNNI3K expression greatly accelerates cardiac dysfunction in mouse models of cardiomyopathy, indicating an important role in modulating disease progression. To further investigate TNNI3K kinase activity in vivo, we have generated transgenic mice expressing both wild-type and kinase-dead versions of the human TNNI3K protein. Importantly, we show that the increased TNNI3K kinase activity induces mouse cardiac remodeling, and its kinase activity promotes accelerated disease progression in a left-ventricular pressure overload model of mouse cardiomyopathy. Using an in vitro kinase assay and proteomics analysis, we show that TNNI3K is a dual-function kinase with Tyr and Ser/Thr kinase activity. TNNI3K expression induces a series of cellular and molecular changes, including a reduction of sarcomere length and changes in titin isoform composition, which are indicative of cardiac remodeling. Using antisera to TNNI3K, we show that TNNI3K protein is located at the sarcomere Z disc. These combined data suggest that TNNI3K mediates cell signaling to modulate cardiac response to stress.

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Published In

J Mol Cell Cardiol

DOI

EISSN

1095-8584

Publication Date

January 2013

Volume

54

Start / End Page

101 / 111

Location

England

Related Subject Headings

  • Ventricular Remodeling
  • Ventricular Dysfunction, Left
  • Stress, Physiological
  • Signal Transduction
  • Sarcomeres
  • Protein Serine-Threonine Kinases
  • Protein Processing, Post-Translational
  • Protein Kinases
  • Protein Isoforms
  • Phosphorylation
 

Citation

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Tang, H., Xiao, K., Mao, L., Rockman, H. A., & Marchuk, D. A. (2013). Overexpression of TNNI3K, a cardiac-specific MAPKKK, promotes cardiac dysfunction. J Mol Cell Cardiol, 54, 101–111. https://doi.org/10.1016/j.yjmcc.2012.10.004
Tang, Hao, Kunhong Xiao, Lan Mao, Howard A. Rockman, and Douglas A. Marchuk. “Overexpression of TNNI3K, a cardiac-specific MAPKKK, promotes cardiac dysfunction.J Mol Cell Cardiol 54 (January 2013): 101–11. https://doi.org/10.1016/j.yjmcc.2012.10.004.
Tang H, Xiao K, Mao L, Rockman HA, Marchuk DA. Overexpression of TNNI3K, a cardiac-specific MAPKKK, promotes cardiac dysfunction. J Mol Cell Cardiol. 2013 Jan;54:101–11.
Tang, Hao, et al. “Overexpression of TNNI3K, a cardiac-specific MAPKKK, promotes cardiac dysfunction.J Mol Cell Cardiol, vol. 54, Jan. 2013, pp. 101–11. Pubmed, doi:10.1016/j.yjmcc.2012.10.004.
Tang H, Xiao K, Mao L, Rockman HA, Marchuk DA. Overexpression of TNNI3K, a cardiac-specific MAPKKK, promotes cardiac dysfunction. J Mol Cell Cardiol. 2013 Jan;54:101–111.
Journal cover image

Published In

J Mol Cell Cardiol

DOI

EISSN

1095-8584

Publication Date

January 2013

Volume

54

Start / End Page

101 / 111

Location

England

Related Subject Headings

  • Ventricular Remodeling
  • Ventricular Dysfunction, Left
  • Stress, Physiological
  • Signal Transduction
  • Sarcomeres
  • Protein Serine-Threonine Kinases
  • Protein Processing, Post-Translational
  • Protein Kinases
  • Protein Isoforms
  • Phosphorylation