Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

A comparison of non-toxin vaccine adjuvants for their ability to enhance the immunogenicity of nasally-administered anthrax recombinant protective antigen.

Publication ,  Journal Article
Gwinn, WM; Johnson, BT; Kirwan, SM; Sobel, AE; Abraham, SN; Gunn, MD; Staats, HF
Published in: Vaccine
March 1, 2013

Development of nasal immunization for human use is hindered by the lack of acceptable adjuvants. Although CT is an effective adjuvant, its toxicity will likely prevent its use in nasal vaccines. This study compared non-toxin adjuvants to CT for their ability to induce protective antibody responses with nasal immunization. C3H/HeN and C57BL/6 mice were immunized with rPA formulated with the following adjuvants: CT, IL-1α, LPS, CpG, Pam3CSK4, 3M-019, resiquimod/R848 or c48/80. Serum and nasal wash cytokine concentrations were monitored 6h post-vaccination as biomarkers for acute activation of the innate immune system. Not all of the adjuvants induced significant changes in innate serum or nasal wash cytokines, but when changes were observed, the cytokine signatures were unique for each adjuvant. All adjuvants except Pam3CSK4 induced significantly increased anti-rPA serum IgG titers in both strains of mice, while only IL-1α, c48/80 and CpG enhanced mucosal anti-rPA IgA. Pam3CSK4 was the only adjuvant unable to enhance the induction of serum LeTx-neutralizing antibodies in C3H/HeN mice while c48/80 was the only adjuvant to induce increased serum LeTx-neutralizing antibodies in C57BL/6 mice. Only CT enhanced total serum IgE in C3H/HeN mice while IL-1α enhanced total serum IgE in C57BL/6 mice. The adjuvant influenced antigen-specific serum IgG subclass and T cell cytokine profiles, but these responses did not correlate with the induction of LeTx-neutralizing activity. Our results demonstrate the induction of diverse innate and adaptive immune responses by non-toxin nasal vaccine adjuvants that lead to protective humoral immunity comparable to CT and that these responses may be influenced by the host strain.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Vaccine

DOI

EISSN

1873-2518

Publication Date

March 1, 2013

Volume

31

Issue

11

Start / End Page

1480 / 1489

Location

Netherlands

Related Subject Headings

  • Virology
  • Vaccines, Synthetic
  • Serum
  • Mice, Inbred C57BL
  • Mice, Inbred C3H
  • Mice
  • Immunoglobulin G
  • Immunity, Mucosal
  • Female
  • Cytokines
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Gwinn, W. M., Johnson, B. T., Kirwan, S. M., Sobel, A. E., Abraham, S. N., Gunn, M. D., & Staats, H. F. (2013). A comparison of non-toxin vaccine adjuvants for their ability to enhance the immunogenicity of nasally-administered anthrax recombinant protective antigen. Vaccine, 31(11), 1480–1489. https://doi.org/10.1016/j.vaccine.2013.01.012
Gwinn, William M., Brandi T. Johnson, Shaun M. Kirwan, Ashley E. Sobel, Soman N. Abraham, Michael D. Gunn, and Herman F. Staats. “A comparison of non-toxin vaccine adjuvants for their ability to enhance the immunogenicity of nasally-administered anthrax recombinant protective antigen.Vaccine 31, no. 11 (March 1, 2013): 1480–89. https://doi.org/10.1016/j.vaccine.2013.01.012.
Gwinn WM, Johnson BT, Kirwan SM, Sobel AE, Abraham SN, Gunn MD, et al. A comparison of non-toxin vaccine adjuvants for their ability to enhance the immunogenicity of nasally-administered anthrax recombinant protective antigen. Vaccine. 2013 Mar 1;31(11):1480–9.
Gwinn, William M., et al. “A comparison of non-toxin vaccine adjuvants for their ability to enhance the immunogenicity of nasally-administered anthrax recombinant protective antigen.Vaccine, vol. 31, no. 11, Mar. 2013, pp. 1480–89. Pubmed, doi:10.1016/j.vaccine.2013.01.012.
Gwinn WM, Johnson BT, Kirwan SM, Sobel AE, Abraham SN, Gunn MD, Staats HF. A comparison of non-toxin vaccine adjuvants for their ability to enhance the immunogenicity of nasally-administered anthrax recombinant protective antigen. Vaccine. 2013 Mar 1;31(11):1480–1489.
Journal cover image

Published In

Vaccine

DOI

EISSN

1873-2518

Publication Date

March 1, 2013

Volume

31

Issue

11

Start / End Page

1480 / 1489

Location

Netherlands

Related Subject Headings

  • Virology
  • Vaccines, Synthetic
  • Serum
  • Mice, Inbred C57BL
  • Mice, Inbred C3H
  • Mice
  • Immunoglobulin G
  • Immunity, Mucosal
  • Female
  • Cytokines