Single-step purification of full-length human androgen receptor.
Publication
, Journal Article
Juzumiene, D; Chang, C-Y; Fan, D; Hartney, T; Norris, JD; McDonnell, DP
Published in: Nucl Recept Signal
2005
The full-length human androgen receptor with an N-terminal biotin acceptor peptide tag was overexpressed in Spodoptera frugiperda cells in the presence of 1 microM dihydrotestosterone. Site-specific biotinylation of BAP was achieved in vivo by co-expression of E. coli biotin holoenzyme synthetase. The androgen receptor was purified by single-step affinity chromatography using Streptavidin Mutein Matrix under native conditions. The resultant protein was active, stable, 95% homogeneous, and we obtained sufficient yield for use in functional and structural studies.
Duke Scholars
Published In
Nucl Recept Signal
DOI
EISSN
1550-7629
Publication Date
2005
Volume
3
Start / End Page
e001
Location
United States
Related Subject Headings
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Juzumiene, D., Chang, C.-Y., Fan, D., Hartney, T., Norris, J. D., & McDonnell, D. P. (2005). Single-step purification of full-length human androgen receptor. Nucl Recept Signal, 3, e001. https://doi.org/10.1621/nrs.03001
Juzumiene, Dalia, Ching-yi Chang, Daju Fan, Tanya Hartney, John D. Norris, and Donald P. McDonnell. “Single-step purification of full-length human androgen receptor.” Nucl Recept Signal 3 (2005): e001. https://doi.org/10.1621/nrs.03001.
Juzumiene D, Chang C-Y, Fan D, Hartney T, Norris JD, McDonnell DP. Single-step purification of full-length human androgen receptor. Nucl Recept Signal. 2005;3:e001.
Juzumiene, Dalia, et al. “Single-step purification of full-length human androgen receptor.” Nucl Recept Signal, vol. 3, 2005, p. e001. Pubmed, doi:10.1621/nrs.03001.
Juzumiene D, Chang C-Y, Fan D, Hartney T, Norris JD, McDonnell DP. Single-step purification of full-length human androgen receptor. Nucl Recept Signal. 2005;3:e001.
Published In
Nucl Recept Signal
DOI
EISSN
1550-7629
Publication Date
2005
Volume
3
Start / End Page
e001
Location
United States
Related Subject Headings
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology