Synergistic and tunable human gene activation by combinations of synthetic transcription factors.
Publication
, Journal Article
Perez-Pinera, P; Ousterout, DG; Brunger, JM; Farin, AM; Glass, KA; Guilak, F; Crawford, GE; Hartemink, AJ; Gersbach, CA
Published in: Nat Methods
March 2013
Mammalian genes are regulated by the cooperative and synergistic actions of many transcription factors. In this study we recapitulate this complex regulation in human cells by targeting endogenous gene promoters, including regions of closed chromatin upstream of silenced genes, with combinations of engineered transcription activator-like effectors (TALEs). These combinations of TALE transcription factors induced substantial gene activation and allowed tuning of gene expression levels that will broadly enable synthetic biology, gene therapy and biotechnology.
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Published In
Nat Methods
DOI
EISSN
1548-7105
Publication Date
March 2013
Volume
10
Issue
3
Start / End Page
239 / 242
Location
United States
Related Subject Headings
- Transfection
- Transcriptional Activation
- Transcription Factors
- Receptor, erbB-2
- Receptor, ErbB-2
- Real-Time Polymerase Chain Reaction
- Prostate-Specific Antigen
- Promoter Regions, Genetic
- Plasmids
- Luciferases
Citation
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Chicago
ICMJE
MLA
NLM
Perez-Pinera, P., Ousterout, D. G., Brunger, J. M., Farin, A. M., Glass, K. A., Guilak, F., … Gersbach, C. A. (2013). Synergistic and tunable human gene activation by combinations of synthetic transcription factors. Nat Methods, 10(3), 239–242. https://doi.org/10.1038/nmeth.2361
Perez-Pinera, Pablo, David G. Ousterout, Jonathan M. Brunger, Alicia M. Farin, Katherine A. Glass, Farshid Guilak, Gregory E. Crawford, Alexander J. Hartemink, and Charles A. Gersbach. “Synergistic and tunable human gene activation by combinations of synthetic transcription factors.” Nat Methods 10, no. 3 (March 2013): 239–42. https://doi.org/10.1038/nmeth.2361.
Perez-Pinera P, Ousterout DG, Brunger JM, Farin AM, Glass KA, Guilak F, et al. Synergistic and tunable human gene activation by combinations of synthetic transcription factors. Nat Methods. 2013 Mar;10(3):239–42.
Perez-Pinera, Pablo, et al. “Synergistic and tunable human gene activation by combinations of synthetic transcription factors.” Nat Methods, vol. 10, no. 3, Mar. 2013, pp. 239–42. Pubmed, doi:10.1038/nmeth.2361.
Perez-Pinera P, Ousterout DG, Brunger JM, Farin AM, Glass KA, Guilak F, Crawford GE, Hartemink AJ, Gersbach CA. Synergistic and tunable human gene activation by combinations of synthetic transcription factors. Nat Methods. 2013 Mar;10(3):239–242.
Published In
Nat Methods
DOI
EISSN
1548-7105
Publication Date
March 2013
Volume
10
Issue
3
Start / End Page
239 / 242
Location
United States
Related Subject Headings
- Transfection
- Transcriptional Activation
- Transcription Factors
- Receptor, erbB-2
- Receptor, ErbB-2
- Real-Time Polymerase Chain Reaction
- Prostate-Specific Antigen
- Promoter Regions, Genetic
- Plasmids
- Luciferases